Objective To explore the fluctuation characteristics of long-term doctor's order workload in pharmacy intravenous admixture services (PIVAS) and build a daily workload fluctuation prediction model and provide reference for the adjustment of PIVAS work mode. Methods Daily workload data of long-term doctor’s orders from PIVAS in the East Campus of Zhongshan Hospital affiliated to Fudan University from July 2020 to June 2021 were selected , and the time series analysis method was used to analyze the workload fluctuation characteristics and a prediction model was established. The accuracy of the model was verified by fitting parameters and prediction results. Results The fluctuation of PIVAS long-term doctor's daily workload data had the characteristics of periodicity, short-term slow rise and irregular variation. The Winters multiplier model was used to fit the series with R² = 0.777, the significance value of Ljung-Box statistic value (P value) was 0.060, and the mean absolute error percentage between the fitted and actual values was 4.45%, indicating that the model fitting accuracy was high. The average relative deviation between the predicted and actual results was 3.81%, indicating that the model prediction was effective. Conclusion The model constructed in this study could be used for the analysis and prediction of long-term doctor's orders workload of PIVAS. However, because the workload of doctor's orders has fluctuations such as periodicity and irregular changes, it is necessary to adjust the working model according to the fluctuation characteristics of the workload and the prediction results to ensure the efficient operation of PIVAS.

Objective To explore the effects of holistic nursing and humanistic care concept on polysomnography ( PSG) of the patients with obstructive sleep apnea hydroplane syndromes ( SAHS) .Methods One hundred patients with SAHS who underwent PSG were divided into the observation group ( that received holistic nursing and humanistic care ) and the control group ( that received routine nursing care ) .The success rate and the cause of failure in the child patients of the 2 groups were recorded for the study .The Anxiety Scale ( SAS) , Depression Scale ( SDS) scores and satisfaction rate were recorded before and after intervention .Results The success rate of the observation group (91.67%) was higher than that of the control group (71.43%).The causes of failure for the mo-nitoring of the observation group included failure of the SpO 2 signal , cease of nasal airflow , disconnection of electrode and inadequate sleep time.After intervention, the SDS scores and SAS scores of the observation group were lower than those of the control group .The rate of nursing satisfaction for the observation group (90%) was higher than that of the control group (P<0.05).Conclusion The application of holistic nursing and humanistic care in sleep apnea hypopnea syndrome patients with polysomnography could improve the success rate of monitoring in child patients , alleviate sleepiness and anxiety and improve the rate of nursing satisfaction .For this rea-son, it is worth further popularization .

Four different strains of Leptospira were subcultured in CGM in contrast with in Korthof medium for three years. The antigenic components of these Leptospira grown in the two medium were analysed by the methods of MAT, CIE and SDS-PAGE. The results showed that:1, the antigenic components of Leptospira were very complex and had more than twenty bands stained with Coomassie brilIiant blue in SDS-FAGE pattern; 2. the antigenicity of Leptospira subcultured in CGM for many generations was relative stability and the same as that in Korthof medium.