Objective To compare postoperative major adverse events in patients with triple-vessel disease undergoing complete versus incomplete revascularization during coronary artery bypass grafting (CABG). Methods A retrospective analysis was conducted on patient data from Tianjin Chest Hospital who underwent primary isolated CABG surgery between 2019 and 2020. Patients were divided into a complete revascularization group and an incomplete revascularization group based on the revascularization status. Inverse probability of treatment weighting (IPTW) was used for risk adjustment. Results A total of 1 419 patients were included in the study, with 1 086 (76.5%) undergoing complete revascularization. IPTW analysis showed that complete revascularization could reduce the incidence of major adverse cardiovascular and cerebrovascular events [HR=0.596, 95%CI (0.404, 0.880), P=0.010] and angina [HR=0.560, 95%CI (0.377, 0.823), P=0.004]. Conclusion In patients with multivessel coronary artery disease, complete revascularization may be associated with improved patient outcomes.

Objective: To analyze the epidemiological characteristics and disease types of extrapulmonary tuberculosis in Ningbo City, Zhejiang Province from 2015 to 2024, so as to provide evidence for optimizing regional tuberculosis prevention and control strategies. Methods: Data on extrapulmonary tuberculosis cases in Ningbo City from 2015 to 2024 were collected through the Ningbo Infectious Disease Surveillance and Early Warning Platform, including gender, age, and disease type. Descriptive analysis was conducted to examine the temporal, regional, and population distributions as well as the distribution of disease types of extrapulmonary tuberculosis in Ningbo City from 2015 to 2024. The trend chi-square test was used to analyze the temporal trends in the incidence and proportion of extrapulmonary tuberculosis. Results: From 2015 to 2024, a total of 11 023 cases of extrapulmonary tuberculosis were reported in Ningbo City, accounting for 25.17% of tuberculosis cases. The proportion showed an increasing trend (P<0.05), with an annual increase rate of 2.15%. The average annual incidence of extrapulmonary tuberculosis was 12.77/105, showing a decreasing trend (P<0.05), with an annual decline rate of 3.18%. In the second quarter, a higher number of extrapulmonary tuberculosis cases were reported, with 3 108 cases, accounting for 28.20%. Beilun District, Yuyao City, and Fenghua District had relatively high average annual incidence of extrapulmonary tuberculosis, at 16.43/105, 15.28/105, and 14.97/105, respectively. The average annual incidence of extrapulmonary tuberculosis in males was lower than in females (10.73/105 vs. 14.93/105, P<0.05). The annual average incidence of extrapulmonary tuberculosis was relatively high among cases aged 65-<75 years, 75-<85 years, and ≥85 years, respectively. The 15-<25 years age group exhibited a higher number of cases of tuberculous peripheral lymphadenopathy, with 253 cases, accounting for 34.90%. The proportion of osteoarticular tuberculosis cases among extrapulmonary tuberculosis cases increased with advancing age (P<0.05). Conclusions From 2015 to 2024, the incidence of extrapulmonary tuberculosis in Ningbo City showed a decreasing trend, while its proportion among all tuberculosis cases showed an increasing trend. Beilun District, Yuyao City, and Fenghua District were high-incidence areas, and females and the elderly were high-risk population. Tuberculous peripheral lymphadenopathy was highly prevalent among young adults, while osteoarticular tuberculosis was highly prevalent among the elderly.

Objective: To investigate the epidemiological and spatio-temporal characteristics of influenza in Jiaxing City, Zhejiang Province, so as to provide insights into perfecting the prevention and control strategies of influenza. Methods: Data of influenza in Jiaxing City from 2019 to 2023 were collected from the Chinese Disease Prevention and Control Information System. Population data of the same period were collected from the Zhejiang Health Information Network Reporting System. The epidemiological characteristics of influenza were analyzed using descriptive analysis. Vector map information was collected from the Open Street Map, and the spatio-temporal clustering characteristics of influenza were analyzed using spatial autocorrelation and spatio-temporal scanning. Results: A total of 181 501 cases of influenza were reported in Jiaxing City from 2019 to 2023, with an average annual reported incidence of 653.93/105. The majority of cases were aged 5 to <15 years (59 785 cases, 32.94%). The majority of the occupations were students (78 239 cases, 43.11%) and pre-school children (33 715 cases, 18.58%). The county (city, district) with the highest reported incidence was Haining City (1 451.70/105), and the town (street) with the highest reported incidence was Chang'an Town (1 932.78/105). Spatial autocorrelation analysis showed that the incidence of influenza in Jiaxing City from 2019 to 2023 had positive spatial correlations (all Moran's I>0, all P<0.05), with a high-high clustering in the southern region. Spatio-temporal scanning analysis showed that there was a spatio-temporal clustering of influenza in Jiaxing City from 2019 to 2023, with the southern region being the primary-type clustering area and the period between November and January of the following year being the clustering time. Conclusion There was a significant spatio-temporal clustering of influenza in Jiaxing City from 2019 to 2023, with winter being the peak season and the southern region being the primary area.

Objective: To investigate the epidemiological characteristics of pertussis in Jiaxing City from 2004 to 2023 and spatio-temporal clustering characteristics from 2022 to 2023, so as to provide insights into formulation of pertussis control measures. Methods: Data of pertussis cases in Jiaxing City from 2004 to 2023 were collected through the Infectious Disease Report Information System of Chinese Disease Prevention and Control Information System. The epidemiological characteristics of pertussis cases in Jiaxing City from 2004 to 2023 were descriptively analyzed, and the spatio-temporal clustering characteristics from 2022 to 2023 were analyzed using spatio-temporal scanning. Results: A total of 478 pertussis cases were reported in Jiaxing City from 2004 to 2023, with an average annual reported incidence of 0.53/105. The reported incidence showed an upward trend from 2004 to 2023 (P<0.05), with the highest in 2022 (3.17/105). Higher incidence of pertussis was reported in June to August (149 cases, 31.17%) and November to December (112 cases, 23.43%). There was no statistically significant difference in the reported incidence between males and females (0.56/105 vs. 0.50/105, P>0.05). The cases aged under one year accounted for the highest proportion, with 199 cases (41.63%). Haining City (0.68/105), Jiashan County (0.64/105) and Tongxiang City (0.60/105) ranked the top three in the reported incidence of pertussis. Spatio-temporal scanning analysis showed that from 2022 to 2023, the primary clustering area of pertussis was centered in Daqiao Town of Nanhu District, covering 27 towns (streets) in Nanhu District, Jiashan County, Xiuzhou District and Pinghu City, and the clustering time was from November to December, 2023. Conclusions The reported incidence of pertussis was at a low level in Jiaxing City, but showed an upward trend from 2004 to 2023. The incidence of pertussis was higher among infants under one year of age, peaked in June to August and November to December, and was concentrated in Nanhu District and its surrounding areas.

Objective To observe the value of conventional ultrasound(US)and contrast-enhanced ultrasound(CEUS)for diagnosing partially cystic papillary thyroid carcinomas(PCPTC).Methods Thirty patients with PCPTC(32 nodules)and 50 patients with benign partially cystic thyroid nodules(PCTN)(52 nodules)were retrospectively enrolled.US and CEUS manifestations were compared between groups,and the efficacy of US and US combined with CEUS for diagnosing PCPTC were analyzed.Results Significant differences of margin,solid part echo,liquid part location,microcalcification and blood flow distribution of the nodules showed with US,as well as enhancement phase,enhancement intensity,enhancement uniformity,peripheral high-enhancement ring and regression mode of lesions shown on CEUS were found between groups(all P＜0.05).The area under the curve(AUC)of US combined with CEUS for diagnosing PCPTC was 0.942,significantly higher than that of US alone(0.750,P＜0.001).Conclusion US had certain value for diagnosing PCPTC.Combining with CEUS could effectively improve diagnostic efficacy of US for PCPTC.

Objective:To construct four recombinant hemagglutinin (HA) antigens from seasonal influenza viruses and evaluate their immunogenicity in mouse models.Methods:HA coding sequences from four seasonal influenza virus strains Wisconsin (H1N1), Darwin (H3N2), Austria (B/Victoria lineage, BV) and Phuket (B/Yamagata lineage, BY) were optimized and synthesized, and then used to construct four recombinant plasmids. Recombinant baculoviruses were obtained through transformation and transfection. The expression of recombinant HA antigens was identified by SDS-PAGE and Western blot. The recombinant HA antigens were purified by nickel column affinity chromatography and intramuscularly administered to BALB/c mice after formulation with Al(OH) 3 or AddaVax adjuvant. Humoral immune responses were assessed by indirect ELISA and hemagglutination inhibition test, while cellular immune responses were evaluated by ELISPOT. Microneutralization test was used to detect the titers of serum antibodies in mice. Statistical analysis was performed using t test or non-parametric rank sum test. Results:PCR amplification and agarose gel electrophoresis confirmed the correct construction of the recombinant bacmids. Western blot showed verified the successful expression of the four recombinant antigens (H1-HA, H3N2-HA, BV-HA, and BY-HA). SDS-PAGE results showed that the purity of all four recombinant HA antigens exceeded 95%. After three-dose immunization, the total IgG levels in mice immunized with the recombinant H1N1-HA, H3N2-HA, or BV-HA formulated with AddaVax adjuvant were higher than those in the corresponding groups immunized with the same recombinant antigen alone (all P<0.05). The secretion levels of IFN-γ, IL-2, and IL-4 in the group receiving the mixture of all four recombinant HA antigens formulated with AddaVax adjuvant were higher than those in the group immunized with a commercial quadrivalent split influenza vaccine (all P<0.01). Results of the microneutralization test showed that the antibody titer in the quadrivalent split influenza vaccine group was 1∶225, whereas the titer in the group immunized with the mixture of four recombinant HA antigens formulated with AddaVax adjuvant could reach up to 1∶1 200. Conclusions:In this study, four recombinant seasonal influenza virus HA antigens are successfully expressed and demonstrated good immunogenicity in mice when formulated AddaVax adjuvant.

Alzheimer's disease(AD), a neurodegenerative disorder associated with aging, is the most prevalent form of dementia.As the aging population continues to expand, AD presents significant health and caregiving challenges for families and society, making it a pressing international public health concern.In recent years, numerous countries have implemented dementia prevention and treatment strategies that emphasize community-based comprehensive approaches.Currently, the community-based AD prevention and treatment model in China is still in the exploratory phase, with community efforts lacking organization.In alignment with China's action plan for advancing dementia prevention and treatment, and to achieve the strategic objective of "healthy aging, " this consensus is based on the principle of three-level prevention and is tailored to the characteristics of AD disease progression.It aims to develop a comprehensive prevention and treatment strategy for AD that is suitable for communities in China, providing technical guidance and support to establish a scientific basis for formulating community AD prevention and treatment models.

Objective:To integrate implicit templates with deep learning techniques, a novel neural network, the tooth-deformable deep implicit network (T-DDIN), was constructed to achieve high-precision shape completion of tooth defects in a personalized manner.Methods:A total of 550 intraoral scan models were collected from patients treated at the Department of Orthodontics and Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University (500 for training and 50 for testing), between March 2022 and March 2024. T-DDIN reconstructed defective tooth morphology using an implicit template and a latent encoding prediction network. During model evaluation, Class Ⅱ cavity defects and occlusal wear defects were simulated in the test set. Morphological restoration was performed using both traditional computer aided design (CAD) methods and the T-DDIN deep learning approach. The two methods were compared based on three-dimensional deviation, occlusal adjustment volumes, cusp angle deviation, and restoration time.Results:The T-DDIN group demonstrated significantly lower three-dimensional deviation for Class Ⅱ cavity defects and occlusal wear restoration [(0.14±0.05) and (0.16±0.09) mm], occlusal adjustment volumes [(0.44±0.03) and (0.49±0.03) mm 3], and difference value of the tooth cusp angles (5.69°±1.90° and 6.04°±0.53°) compared to the traditional CAD group (both P<0.001). No significant differences were observed within the T-DDIN group between the two defect types in terms of three-dimensional deviation ( P=0.098) or occlusal adjustment volume ( P=0.154) or difference value of the tooth cusp angles ( P=0.196). However, in the traditional CAD group, three-dimensional deviation, occlusal adjustment volume and difference value of the tooth cusp angles was significantly higher in occlusal wear restorations than in Class Ⅱ cavity defects restorations ( P<0.001). The T-DDIN group, which involved Class Ⅱ cavity defects and occlusal wear, demonstrated significantly less recovery time of morphology (37.2±7.7) and (39.4±6.2) s compared to the traditional CAD group ( P<0.001). Conclusions:T-DDIN demonstrated superior stability and accuracy in morphological reconstruction for various types of dental defects while significantly reducing restoration time.

Objective:To analyze the effects and mechanisms of ginseng polysaccharide in myocardial fibrosis and endothelial function in coronary heart disease model rats based on mitogen activated extracellular signal regulated kinase (MEK) and extracellular signal regulated kinase (ERK) signaling pathways.Methods:Totally 60 SD rats were divided into control group (10 rats) and model group (50 rats). A coronary heart disease model was established in modeling rats. The successfully modeled rats were divided into model group, drug control group, ginseng polysaccharide high- and low-dosage groups, with 10 rats in each group. The drug control group was orally administered with 2 mg/kg Lipitor solution, while the ginseng polysaccharide low- and high-dosage groups were orally administered with 100 and 200 mg/kg ginseng polysaccharide solution. The control group and model group were orally administered with equal volumes of physiological saline solution once a day for one week. HE staining and Masson staining were used to observe the morphological changes and pathological morphology of myocardial tissue. ELISA was used to detect the levels of NO, eNOS, ET-1, ADMA, TNF-α, CRP, and IL-6. Immunochemiluminescence was used to detect the expression levels of CK-MB and cTnl. Real time fluorescence quantification was used to identify the expression of MEK and ERK. Western blot was used to detect the expression of MEK, ERK, p-MEK, and p-ERK in myocardial tissue.Results:Compared with the model group, the levels of serum NO and eNOS increased in the drug control group and ginseng polysaccharide low- and high-dosage groups ( P<0.05), while the levels of ET-1, ADMA, TNF-α, CRP, IL-6, CK-MB, and cTnl decreased ( P<0.05), and the mRNA and protein expressions of MEK and ERK in myocardial tissue decreased ( P<0.05). Conclusions:Ginseng polysaccharides can improve the degree of myocardial fibrosis in rats, alleviate endothelial function and myocardial injury. Its mechanism may be related to the inhibition of the MEK/ERK signaling pathway.

Objectives:To evaluate the effect of extended single prone positioning ventilation on survival and weaning rate of acute respiratory distress syndrome (ARDS) patients supported by VV-ECMO.Methods:ARDS patients supported by VV-ECMO admitted to Jinhua Central Hospital, the Fourth Affiliated Hospital of Zhejiang University School of Medicine and the First Hospital of Jiaxing from September 2014 to May 2025 were retrospectively enrolled into the study. The clinical data, ECMO and ventilator related parameters and outcomes of the patients were collected. The patients were divided into the extended prone positioning group and prone positioning group according to whether the duration of prone position ventilation was greater than 24 h. The clinical data of the two groups were compared to explore the effects on 30-day survival in-hospital survival and ECMO withdraw rate of these patients. Multivariate logistic regression analysis was used to explore the relationship between the duration of single prone position ventilation and the success of ECMO weaning, 30-day survival and hospital survival.Results:Total of 163 ARDS patients supported by VV-ECMO receiving prone positioning ventilation were included in study, 64 in extended prone positioning group and 72 in prone positioning group. The 30-day survival (54.7% vs. 52.8%) in-hospital survival (51.6% vs. 48.6%) and ECMO withdraw rate (57.8% vs. 61.1%) between the two groups were not statistically different ( P>0.05) as well as the duration of ECMO support [12(10,15)d vs. 11(10,13)d] the duration of ventilation [16(13,18)d vs. 16(12,18)d] the duration of ICU stay [26(15,32)d vs. 26(19,29)d] and the duration of hospital stay [32(15,42)d vs. 34(28,35)d]. Logistic regression analysis revealed that the duration of each prone position ventilation was not associated with successful weaning ( OR=0.979, 95% CI:0.952-1.006), 30-day survival ( OR=1.015, 95% CI: 0.975-1.056) and hospital survival ( OR=1.014, 95% CI: 0.974-1.055) even after adjusting for the severity of illness, age, and type of pneumonia. Conclusions:For ARDS patients supported by VV-ECMO, extended single prone positioning ventilation for more than 24 hours neither increase 30-day survival in-hospital survival and successful ECMO weaning rate, nor shorten ECMO support duration.