Objective:To investigate the effect of miRNA-34b/c-5p on the expression of neurokinin 1 receptor-truncated(NK1R-Tr)in breast cancer and the effect of miRNA-34b/c-5p and NK1R-Tr on the migration and invasion abilities of breast cancer cells.Methods:Real-time fluorescence quantitative PCR(RT-PCR)was used to detect the expression of miRNA-34b/c-5p and NK1R-Tr in 50 breast can-cer specimens that were collected at Tianjin Medical University Cancer Institute&Hospital from February to May 2013.Western blot analysis was performed to detect the expression of miRNA-34b/c-5p and NK1R-Tr in breast cancer cell lines MDA-MB-231 and MCF-7. Scratch and Transwell assays were carried out to explore the effects of miRNA-34b/c-5p and NK1R-Tr on the migration and invasion abilities of MDA-MB-231 cells.Results:The expression of miRNA-34b/c-5p and NK1R-Tr in breast cancer tissues and cells were signifi-cantly negatively correlated.The relative expression of NK1R-Tr in breast cancer patients with lymph node metastasis was 5.75,and the relative expression of NK1R-Tr in patients with non-lymph node metastasis was 4.29.The relative expression was significantly dif-ferent between the two groups(P=0.026).Overexpression of miRNA-34b/c-5p and knockdown of NK1R-Tr could significantly inhibit the migration and invasion abilities of MDA-MB-231 cells(all P<0.001).Conclusions:The expression of miRNA-34b/c-5p and NK1R-Tr was significantly negatively correlated.MiRNA-34b/c-5p and NK1R-Tr might be potential therapeutic targets for inhibiting the invasion of breast cancer cells.

Objective To investigate and analyze the anomaly characteristics of metabolic indexes of the active flying personnel with different BMI and different age and to provide the basis for health management of the flying personnel.Methods The cross-sectional study was applied to the collected data of 339 flying personnel who were hospitalized or in outpatient health examination in the 454th Hospital of PLA.The data included height,weight,blood pressure (BP),the serum levels of fasting plasma glucose (FPG),triglyceride (TG),total cholesterol (TC),high-density lipoprotein cholesterol (HDL-C),low-density lipoprotein cholesterol (LDL-C) and uric acid (UA).Body mass index (BMI) of the flying personnel was calculated.The flying personnel were divided into 2 BMI groups (BMI＜25 kg/m2 and BMI≥25 kg/m2) and 3 age groups (＜35 yr,35-45 yr,and ＞45 yr).The differences of all the indexes between different BMI and age groups were analyzed.Results The abnormal rate of the indexes in flying personnel respectively was BMI:42.77 ％,TG:22.71％,UA:8.26％,BP:7.67％,HDL-C:7.08％,LDL-C:6.49％,TC:4.42％ and FPG:1.77％.The abnormal rates of BP and TG in BMI≥25 kg/m2 group (13.79％,32.41％) were higher than those in BMI＜25 kg/m2 group (3.09％,15.46％) (x2 =13.417,13.580,P＜0.01).The average serum level of FPG,TG,TC and LDL-C in BMI≥25 kg/m2 group [(5.20-k0.56),(1.55±0.92),(4.86±0.84),(3.09±0.69) mmol/L] were higher than those in BMI＜25 kg/m2 group [(5.02±0.40),(1.28± 1.17),(4.67±0.87),(2.85±0.73) mmol/L],but the average serum level of HDL-C [(1.20±0.25) mmol/L] was lower than that in BMI＜25 kg/m2 group [(1.33±0.31) mmol/L] (t=2.028-4.058,P＜0.01 or P＜0.05).There was significant difference on the abnormal rates of FPG,TG and BMI among 3 age groups (x2 =7.158-12.408,P＜0.01 or 0.05).The abnormal rate of FPG (8.33％) in ＞45 yr group was higher than that in 35-45 yr group(0.00％) (P＜0.05).The abnormal rate of TG in ＜35 yr group (17.34％) was lower than that in other 2 groups (37.50％,26.76％) (P＜0.05).The abnormal rate of BMI in 35-45 yr group (52.82％) was higher than that in ＜35 yr group (33.53％) (P＜0.05).There were significant differences on the average serum level of FPG,TG and BMI in 3 age groups (F=3.343-3.801,P＜0.05).The average level of FPG in ages ＞45 yr group [(5.34±0.90) mmol/L] was higher than that in other 2 age groups [(5.07±0.44),(5.08±0.42) mmol/L,(P＜0.05)].The level of TG in ＞45 yr group [(1.86± 1.40) mmol/L] was higher than that in ＜35 yr group [(1.29±1.16) mmol/L] (P＜0.05).The BMI in 35-45 yr group [(25.01±2.18)kg/m2] was higher than that in ＜35 yr group [(24.38± 2.23)kg/m2] (P ＜0.05).Conclusions Overweight or obesity is a common problem in flying personnel.Overweight or obesity is closely related to metabolic disturbance.Attention should be paid to the health management and education of flying personnel,and the treatment plan should be adopted according to each patient's own situation.Promoting healthy lifestyle,learning to relieve psychological pressure,paying attention to weight control and taking corresponding active prevention measures are recommended to flying personnel.

Objective To investigate and analyze the anomaly characteristics of metabolic indexes of the active flying personnel with different BMI and different age and to provide the basis for health management of the flying personnel.Methods The cross-sectional study was applied to the collected data of 339 flying personnel who were hospitalized or in outpatient health examination in the 454th Hospital of PLA.The data included height,weight,blood pressure (BP),the serum levels of fasting plasma glucose (FPG),triglyceride (TG),total cholesterol (TC),high-density lipoprotein cholesterol (HDL-C),low-density lipoprotein cholesterol (LDL-C) and uric acid (UA).Body mass index (BMI) of the flying personnel was calculated.The flying personnel were divided into 2 BMI groups (BMI＜25 kg/m2 and BMI≥25 kg/m2) and 3 age groups (＜35 yr,35-45 yr,and ＞45 yr).The differences of all the indexes between different BMI and age groups were analyzed.Results The abnormal rate of the indexes in flying personnel respectively was BMI:42.77 ％,TG:22.71％,UA:8.26％,BP:7.67％,HDL-C:7.08％,LDL-C:6.49％,TC:4.42％ and FPG:1.77％.The abnormal rates of BP and TG in BMI≥25 kg/m2 group (13.79％,32.41％) were higher than those in BMI＜25 kg/m2 group (3.09％,15.46％) (x2 =13.417,13.580,P＜0.01).The average serum level of FPG,TG,TC and LDL-C in BMI≥25 kg/m2 group [(5.20-k0.56),(1.55±0.92),(4.86±0.84),(3.09±0.69) mmol/L] were higher than those in BMI＜25 kg/m2 group [(5.02±0.40),(1.28± 1.17),(4.67±0.87),(2.85±0.73) mmol/L],but the average serum level of HDL-C [(1.20±0.25) mmol/L] was lower than that in BMI＜25 kg/m2 group [(1.33±0.31) mmol/L] (t=2.028-4.058,P＜0.01 or P＜0.05).There was significant difference on the abnormal rates of FPG,TG and BMI among 3 age groups (x2 =7.158-12.408,P＜0.01 or 0.05).The abnormal rate of FPG (8.33％) in ＞45 yr group was higher than that in 35-45 yr group(0.00％) (P＜0.05).The abnormal rate of TG in ＜35 yr group (17.34％) was lower than that in other 2 groups (37.50％,26.76％) (P＜0.05).The abnormal rate of BMI in 35-45 yr group (52.82％) was higher than that in ＜35 yr group (33.53％) (P＜0.05).There were significant differences on the average serum level of FPG,TG and BMI in 3 age groups (F=3.343-3.801,P＜0.05).The average level of FPG in ages ＞45 yr group [(5.34±0.90) mmol/L] was higher than that in other 2 age groups [(5.07±0.44),(5.08±0.42) mmol/L,(P＜0.05)].The level of TG in ＞45 yr group [(1.86± 1.40) mmol/L] was higher than that in ＜35 yr group [(1.29±1.16) mmol/L] (P＜0.05).The BMI in 35-45 yr group [(25.01±2.18)kg/m2] was higher than that in ＜35 yr group [(24.38± 2.23)kg/m2] (P ＜0.05).Conclusions Overweight or obesity is a common problem in flying personnel.Overweight or obesity is closely related to metabolic disturbance.Attention should be paid to the health management and education of flying personnel,and the treatment plan should be adopted according to each patient's own situation.Promoting healthy lifestyle,learning to relieve psychological pressure,paying attention to weight control and taking corresponding active prevention measures are recommended to flying personnel.

Objective To discuss the short-term currative effect of horizontal osteotomy across injured vertebral forⅤdegree old frac-ture-dislocations of thoracolumbar spine.Methods The data of 4 patients who received horizontal osteotomy across injured vertebral internal fixation in our hospital from April 2010 to October 2012 were retrospectively analyzed,of which 1 case with T8-9 fracture dislocation,2 cases with T10-11 fracture dislocation,1 case with T11-12 fracture dislocation.The Franke1 classification of all patients were grade A.Results All 4 cases were achieved completely fracture reduction and followed-up for 2-14 months(an average of 7.4 months),who could get sitting posi-tion with the support of brace 1 week after operation.The postoperative follow-up indicated that all 4 cases got fusion,but there was no improve-ment for Franke1 classification.Conclusion The method of horizontal osteotomy across injured vertebral forⅤdegree old fracture-dislocations of thoracolumbar spine has good short-term clinical effect,with the advantages of shorter operation time,less bleeding and good reduction effect.

Objective:To determine the expression of the full-length (NK1R-FL) and truncated (NK1R-Tr) neurokinin-1 receptor (NK1R) and the neurokinin-2 receptor (NK2R) in breast cancer tissues and cell lines, as well as to study the effects of the NK1R and NK2R antagonists on the growth of breast cancer cells. Methods:Immunohistochemistry and Western blot assays were used to detect NK1R, NK1R-FL, and NK2R expression in clinical samples of primary breast cancer tissue, benign lesions, and normal breast tissue, as well as in different breast cancer cell lines. Cell proliferation and soft agar growth tests were performed on cells treated with the NK1R and NK2R antagonists to study the ectopic overexpression of NK1R-FL and NK1R-Tr in breast cancer cell lines. Results:Total NK1R expression was detected in the breast cancer tissues, benign lesions, and normal breast tissues. Compared with the normal breast epithe-lia and benign breast lesions, the expression levels of NK1R-FL and NK2R decreased in the carcinoma. These changes were also relat-ed to the carcinoma type, histological grade, lymph node metastasis, HER2 and Ki-67 expression, and estrogen and progesterone recep-tors in breast cancer. The expression levels of NK1R-FL and NK2R were high in the HBL-100 breast cell lines of para-neoplastic tis-sues, but NK1R-Tr expression was low. The MDA-MB-231, T-47D, and MCF-7 cells only expressed NK1R-Tr. NK1R-Tr or NK1R-FL overexpression caused the decreased inhibition rate or increased levels of the NK1R and NK2R antagonists in the breast cancer cells. Conclusion:NK1R-FL and NK2R are co-expressed in normal cells. NK1R-Tr is highly expressed in breast cancer cells and exerts nega-tive feedback to regulate NK1R-FL and NK2R expression in all cells, especially cancer cells.

Objective To investigate the effect of dexmedetomidine combined anesthesia on postoperative delirium in the elderly patients undergoing hip joint replacement.Methods One hundred and sixty ASA Ⅱ or Ⅲ patients,aged 75-85 yr,with a body mass index of 20-25 kg/m2,scheduled for elective total hip joint replacement under general anesthesia,were randomly divided into 2 groups (n =80 each):sevoflurane-fentanyl control group (group C) and dexmedetomidine-sevoflurane-fentanyl group (group D).Dexmedetomidine was intravenously infused at 0.2 μg· kg-1 · h-1 until the end of operation after a loading dose of 0.6μg/kg over 15 min before induction of anesthesia in group D,while the equal volume of normal saline was infused in group C.Anesthesia was induced with iv injection of fentanyl 2-4 μg/kg and etomidate 0.1-0.3 mg/kg at 5 min after the end of administration of the loading dose.Tracheal intubation was facilitated with cisatracurium 0.15 mg/kg and the patients were mechanically ventilated.Anesthesia was maintained with target-controlled inhalation of sevoflurane (the end-tidal concentration 1％-3％) and intermittent iv boluses of fentanyl 0.05 mg.BIS was maintained at 40-60 during operation.The consumption of etomidate,fentanyl,and sevoflurane,agitation during emergence from anesthesia,and postoperative delirium within 24 h after operation were recorded.Results Compared with group C,the consumption of etomidate,fentanly,and sevoflurane was significantly reduced and the incidences of agitation during emergence from anesthesia and postoperative delirium within 24 h after operation were significantly decreased in group D (P ＜0.05).Conclusion Dexmedetomidine combined anesthesia can reduce the occurrence of postoperative delirium in the elderly patients undergoing hip joint replacement.

Objective To evaluate the effect of dexmedetomidine on intraoperative wake-up test in patients undergoing scoliosis surgery.Methods Sixty ASA Ⅰ patients,aged 13-18 yr,scheduled for elective scoliosis surgery,were randomly divided into 2 groups (n =30 each) ∶ sufentanil group (group S) and dexmedetomidine combined with sufentanil group (group DS).Anesthesia was induced with sufentanil,etomidate and cisatracurium,and maintained with target-controlled inhalation of sevoflurane combined with target-controlled infusion of sufentanil in both groups.In group DS,dexmedetomidine 0.2 μg·kg-1 · h-1 was infused during operation,while the equal volume of normal saline was given instead in group S.Before the wake-up test,cisatracurium infusion was stopped,the end-tidal concentration of sevoflurane was adjusted to 0,and the target effect-site concentration of sufentanil was adjusted to 0.1 and 0.08 ng/ml in groups S and DS,respectively.The wake-up test was performed 5 min later.The wake-up time and bucking/restlessness and cardiovascular events during the wake-up test were recorded.The occurrence of intraoperative awareness was recorded in the patients who were followed up after operation.Results No cardiovascular events occurred during the wake-up test in group DS.The wake-up time was significantly shorter and the incidences of cardiovascular events and bucking/restlessness were significantly lower in group DS than in group S (P ＜ 0.05).No intraoperative awareness occurred in the patients.Conclusion Dexme-detomidine can be used for intraoperative wake-up test in patients undergoing scoliosis surgery,with shorter wakeup time,stable hemodymamics and fewer adverse reactions.

Objective To investigate the risk factors for postoperative delirium in patients undergoing spine operation under total intravenous anesthesia. Methods Two hundred patients undergoing elective spine operation were included in this study. Patients' perioperative informations were recorded. The diagnosis of delirium was based on Confusion Assessment Method (CAM). The patients were divided into 2 groups according to the occurrence of delirium within 3 days after operation: postoperative delirium group and no postoperative delirium group. Multivariate logistic regression was used to analyze the risk factors for postoperative delirium. Results Postoperative delirium developed in 39 patients during the first three postoperative days. The incidence of postoperative delirium was 19.5%. The logistic stepwise regression analysis indicated that the risk factors for delirium included age, intraoperative blood loss and alcohol abuse. Conclusion Age, large amount of intraoperative blood loss and alcohol abuse are risk factors for postoperative delirium in patients undergoing spine operation under total intravenous anesthesia.

Objective To investigate the risk factors for postoperative delirium in patients undergoing noncardiac surgery. Methods From September-December 2009, 480 patients undergoing elective noncardiac surgery were included in this study. The criteria for enrolment of the patients in this study were (1) age ≥ 65 yr,(2) age ＜ 65 yr if associated with coronary artery disease, hypertension, diabetes mellitus, brain injury, cerebral hemorrhage, cerebral infarct, emphysema, chronic bronchitis; duration of operation ≥ 3 h and intraoperative blood loss was expected to exceed 20% of blood volume. The diagnosis of delirium was based on Confusion Assessment Method (CAM). Multivariate logistic regression was used to analyze the risk factors for postoperative delirium.Results Postoperative delirium developed in 79 patients during the first three postoperative days. The incidence of postoperative delirium was 16.5%. The logistic stepwise regression analysis indicated that the risk factors for delirium included advanced age, method of anesthesia, long operation, postoperative pain, emphysema and alcholic. Conclusion Age, method of anesthesia, long operation, postoperative pain, emphysema and alcholic are risk factors for postoperative delirium in patients undergoing noncardiac surgery.

Objective To investigate the effect of propofol anesthesia on stress response and immune function of laproscopic cholecystectomy.Methods Corticosterone,glucose,complement concentrations in plasma of 20 ASAⅠ～Ⅱ patients under propofol combined anesthesia undergoing elective laproscopic cholecystectomy,were respectively determined before anesthesia,operation,gallbladder ablation and after de-intubation.Results After anesthesia,blood glucose increased while C3 and CH50 decreased gradually(P