This paper introduces professor WU Rongzu's clinical experience in using Fuyang Tongmi Decoction （扶阳通秘汤， FTD） to treat irritable bowel syndrome with constipation （IBS-C）. It is believed that yang qi depletion， water cold， earth dampness， and wood constraint are the key pathogenesis.The treatment principle is warming water， drying the earth and venting wood， with the basic formula FTD adjusted according to the symptoms. This approach aims to transport the qi movement of the middle jiao （焦） and support the recovery of intestinal function of directing turbidity downward， providing a treatment strategy for IBS-C caused by yang deficiency.

Objective:The whole genome of one porcine sapelovirus (PSV) strain from mosquitoes captured in Weishan county, Shandong province was sequenced and analyzed its genetic characteristics.Methods:Mosquitoes collected in pigpen in Weishan county of Shandong province in 2021 were homogenized and inoculated on C6/36 and BHK21 cell lines for virus isolation. Virus RNA was extracted from the positive culture and the whole genome was sequenced by next generation sequencing. Phylogenetic analysis, similarity comparison and recombination analysis were conducted via MEGA 7.0.26, BioEdit 7.0.9.0 and Simplot 3.5.1.Results:A PSV strain 265/SD was isolated from Culex tritaeniorhynchus. The clear cytopathic effects characterized by rounding, shrinkage and detachment of cells were observed on BHK21 cells. The whole genome of this study strain was 7 558 nt in length. Phylogenetic analysis based on VP1 sequences indicated that strain 265/SD belonged to traditional genotype. Different positions of 265/SD in the VP1 and 3D phylogenetic trees suggested the recombinant potential. Similarity plot revealed the existence of recombination but the exact origins remained unknown. The similarity analysis of VP1 regions showed that strain 265/SD had low nucleotide identity (77.3%) and amino acid identity (80.3%) with another strain SD/CHN/2016 from Shandong province. Conclusions:Our data enriched the whole genome information of PSV in Shandong province and provided scientific basis for prevention and control of PSV.

Objective:The whole genome of one porcine sapelovirus (PSV) strain from mosquitoes captured in Weishan county, Shandong province was sequenced and analyzed its genetic characteristics.Methods:Mosquitoes collected in pigpen in Weishan county of Shandong province in 2021 were homogenized and inoculated on C6/36 and BHK21 cell lines for virus isolation. Virus RNA was extracted from the positive culture and the whole genome was sequenced by next generation sequencing. Phylogenetic analysis, similarity comparison and recombination analysis were conducted via MEGA 7.0.26, BioEdit 7.0.9.0 and Simplot 3.5.1.Results:A PSV strain 265/SD was isolated from Culex tritaeniorhynchus. The clear cytopathic effects characterized by rounding, shrinkage and detachment of cells were observed on BHK21 cells. The whole genome of this study strain was 7 558 nt in length. Phylogenetic analysis based on VP1 sequences indicated that strain 265/SD belonged to traditional genotype. Different positions of 265/SD in the VP1 and 3D phylogenetic trees suggested the recombinant potential. Similarity plot revealed the existence of recombination but the exact origins remained unknown. The similarity analysis of VP1 regions showed that strain 265/SD had low nucleotide identity (77.3%) and amino acid identity (80.3%) with another strain SD/CHN/2016 from Shandong province. Conclusions:Our data enriched the whole genome information of PSV in Shandong province and provided scientific basis for prevention and control of PSV.

Lateral Ligament, Ankle/injuries* ; Ankle Joint

New threats posed by the emerging circulating variants of SARS-CoV-2 highlight the need to find conserved neutralizing epitopes for therapeutic antibodies and efficient vaccine design. Here, we identified a receptor-binding domain (RBD)-binding antibody, XG014, which potently neutralizes β-coronavirus lineage B (β-CoV-B), including SARS-CoV-2, its circulating variants, SARS-CoV and bat SARSr-CoV WIV1. Interestingly, antibody family members competing with XG014 binding show reduced levels of cross-reactivity and induce antibody-dependent SARS-CoV-2 spike (S) protein-mediated cell-cell fusion, suggesting a unique mode of recognition by XG014. Structural analyses reveal that XG014 recognizes a conserved epitope outside the ACE2 binding site and completely locks RBD in the non-functional "down" conformation, while its family member XG005 directly competes with ACE2 binding and position the RBD "up". Single administration of XG014 is effective in protection against and therapy of SARS-CoV-2 infection in vivo. Our findings suggest the potential to develop XG014 as pan-β-CoV-B therapeutics and the importance of the XG014 conserved antigenic epitope for designing broadly protective vaccines against β-CoV-B and newly emerging SARS-CoV-2 variants of concern.
Angiotensin-Converting Enzyme 2 ; Antibodies, Neutralizing ; Antibodies, Viral ; COVID-19 ; Epitopes ; Humans ; SARS-CoV-2/genetics* ; Spike Glycoprotein, Coronavirus/genetics*

No abstract available.
Arthritis, Rheumatoid* ; Humans

This study aimed at exploring a fast method to accurately identify the medicinal insect Vespa mandarinia Smith from its adulterants using DNA barcode and COI sequences.The extracted DNAs from V.mandarinia and its adulterants V.soror were amplified by polymerase chain reaction (PCR) and sequenced bilaterally based on COI barcode sequence investigation.The information of the COI sequences of V.mandarinia and V.soror were gathered from GenBank.All the sequences were compared and analyzed,and their intraspecific and interspecific genetic distances were calculated using MEGA 6.06.In addition,the phylogenetic tree was established with neighbor-joining (NJ) method.As a result,the COI sequences of V.mandarinia and V.soror were successfully amplified.The minimum interspecific distance between V.mandarinia and its adulterants was 0.152 ± 0.017,being considerably larger than the maximal intraspecific distance between V.mandarinia,0.009±0.004.The constructed phylogenetic tree showed an independent branch for each species.It was concluded that the DNA barcode based on COI sequence can efficiently identify V.mandarinia and its adulterants.This study provided an innovative tool for the quality control and market regulation of Chinese materia medica,securing the safe medication of V.mandarinia.

Objective To observe the effect of swallowing training exercise for promoting rehabilitation of swallowing dysfunction after skull base tumor surgery. Methods We averagedly divided 86 patients with swallowing dysfunction into experimental group and control randomly, and the patinets of control group received routine treatment and care, the patients of experimental group not only on the basis of routine treatment and but also received a self-designed swallowing training exercise. Results The effective rate of swallowing function rehabilitation was 100. 0% in the experimental group while it was 65. 1% in the control group (U=360. 5,P<0. 01). The length of hospitalization in the experimental group was lower than that of the control group. Conclusions Swallowing training exercise can promote functional recovery, shorten hospital stay and improve the quality of life of patients.

Objective To investigate the feasibility of spatiotemporal image correlation(STIC)to assess left ventricular volume in normal fetuses.Methods The volume data acquisition of 162 normal fetuses ranging between 22 and 38 weeks of gestation was achieved by automatic sweep scan.The virtual organ computer-aided analysis(VOCAL Ⅱ)was then used to evaluate end-systolic and end-diastolic volumes of left ventricle and compared with that obtained by M-mode Teichholz formula and 2D biplane Simpson's method.Results Twenty-six fetuses with poor STIC image and 2 twin pregnancies were excluded,the study subjects consisted of 134 singleton fetuses.The highest concordance correlation(r =0.968,for EDV,r =0.956 for ESV;P =0.000)was noted between 2D biplane Simpson' s and STIC VOCAL Ⅱ values with a small bias(0.282 ml for EDV,0.117 ml for ESV).The Bland-Altman analysis showed that STIC VOCAL Ⅱ had the best agreement than M-mode Teichholz formula and 2D biplane Simpson's method.The result showed that there were significant difference between Mmode Teichholz formula and Simpson's method in EDV and ESV(P＜0.05).There were significant differences between M-mode Teichholz formula and STIC VOCAL Ⅱ(P＜0.05).There were no significant differences between STIC VOCAL Ⅱ and Simpson' s method in EDV and ESV.Conclusions There is a good agreement between left ventricular volumes measured either by STIC VOCAL Ⅱ or by 2D biplane Simpson's method.STIC is a novel technique that can be more accurate than conventional Methods in quantification of fetal left ventricular volumes.All the volume data acquisition is achieved by postprocessing facilities.STIC promises to become a new method for left ventricular volume quantification.

Objective To evaluate the clinical significance and cardiac function of fetuses with intracardiac hyperechogenic focus(ICEF) by fetal echocardiography. Methods Fetal echocardiograms were performed on 1291 fetuses for evaluation of fetal heart structures and rhythms. Echocardiographic characteristics of ICEF were investigated. Fetal systolic and diastolic functions of 399 fetuses with an isolated ICEF were compared with 128 normal fetuses using conventional two-dimensional, M-mode, pulsed Doppler echocardiography and myocardial Doppler tissue imaging(DTI). Results The prevalence of ICEF was 34. 7% (448/1291). Twenty-four(5.4%) cases were found with cardiovascular malformations. There was 1 (0.22%) case of ICEF fetus associated with chromosomal abnormality (trisomy 21 ). Comprehensive fetal echocardiographic data analysis showed no significant differences in heart dimensions, global cardiac functions,and myocardial performances in the two groups( P ＞0.05). Conclusions An isolated ICEF in the fetal heart is not associated with increased risk for chromosome abnormalities. Fetuses with ICEF do not indicate impaired left ventricular systolic or diastolic function, therefore having no hemodynamic significance.