Objective:To identify the risk factors for deep vein thrombosis (DVT) in lower limbs after total hip arthroplasty (THA) in elderly patients.Methods:Medical records of elderly patients, aged ≥ 65 yr, of American Society of Anesthesiologists Physical Status classification Ⅰ-Ⅳ, who underwent unilateral THA from June 2013 to May 2019, were retrospectively collected. Postoperative screening for DVT was performed using Doppler ultrasound, and the patients were divided into non-DVT group and DVT group based on the results. Logistic regression analysis was then employed to identify the risk factors associated with DVT.Results:The results of logistic regression analysis revealed that female, preoperative C-reaction protein concentration>8 mg/L, intraoperative hypotension, and postoperative transfer to intensive care unit were independent risk factors for DVT after THA in elderly patients ( P<0.05). Conclusions:Female, preoperative C-reaction protein concentration>8 mg/L, intraoperative hypotension, and postoperative transfer to intensive care unit are independent risk factors for DVT in lower limbs following THA in elderly patients.

Objective:To identify the risk factors for deep vein thrombosis (DVT) in lower limbs after total hip arthroplasty (THA) in elderly patients.Methods:Medical records of elderly patients, aged ≥ 65 yr, of American Society of Anesthesiologists Physical Status classification Ⅰ-Ⅳ, who underwent unilateral THA from June 2013 to May 2019, were retrospectively collected. Postoperative screening for DVT was performed using Doppler ultrasound, and the patients were divided into non-DVT group and DVT group based on the results. Logistic regression analysis was then employed to identify the risk factors associated with DVT.Results:The results of logistic regression analysis revealed that female, preoperative C-reaction protein concentration>8 mg/L, intraoperative hypotension, and postoperative transfer to intensive care unit were independent risk factors for DVT after THA in elderly patients ( P<0.05). Conclusions:Female, preoperative C-reaction protein concentration>8 mg/L, intraoperative hypotension, and postoperative transfer to intensive care unit are independent risk factors for DVT in lower limbs following THA in elderly patients.

Single-cell genomics provides substantial resources for dissecting cellular heterogeneity and cancer evolution. Unfortunately, classical DNA amplification-based methods have low throughput and introduce coverage bias during sample preamplification. We developed a single-cell DNA library preparation method without preamplification in nanolitre scale (scDPN) to address these issues. The method achieved a throughput of up to 1800 cells per run for copy number variation (CNV) detection. Also, our approach demonstrated a lower level of amplification bias and noise than the multiple displacement amplification (MDA) method and showed high sensitivity and accuracy for cell line and tumor tissue evaluation. We used this approach to profile the tumor clones in paired primary and relapsed tumor samples of hepato-cellular carcinoma (HCC). We identified three clonal subpopulations with a multitude of aneuploid alterations across the genome. Furthermore, we observed that a minor clone of the primary tumor containing additional alterations in chro-mosomes 1q, 10q, and 14q developed into the dominant clone in the recurrent tumor, indicating clonal selection during recurrence in HCC. Overall, this approach provides a comprehensive and scalable solution to understand genome hetero-geneity and evolution.