OBJECTIVE This study evaluates the role of Gelation metnacrylate(GelMA)hydrogel loaded with hPT H(3-34)(29-34)short peptide in the healing of chronic infectious skin wounds in diabetic rats.METHODS Ex-perimental rats were divided into control group,GelMA group and GelM A+hPTH(3-34)(29-34)group,with 15 rats in each group.Diabetic rat models were prepared,and chronic infectious skin wounds were constructed.Differences in wound area changes,wound healing-related indicator[type Ⅰ collagen α1 chain(COL1A1),α-smooth muscle actin(α-SMA),platelet endothelial cell adhesion molecule-1(CD31),matrix metalloproteinase-9(MMP9)and cyclooxygenase-2(Cox2)]expression and migration ability of fibroblasts in vitro were compared a-mong the groups.RESULTS Compared with the control group,the wound area of rats in the GelMA group de-creased on Day 7 and Day 14(P＜0.05),and the wound area of rats in the GelM A+hPTH(3-34)(29-34)group decreased on Day 3,Day 7 and Day 14(P＜0.05).Compared with the GelMA group,the wound area of rats in the GelM A+hPTH(3-34)(29-34)group decreased on Day 3,Day 7 and Day 14(P＜0.05).Compared with the control group,on Day 7 after modeling,the expression of COL1A1,α-SMA and CD31 in rats was upregulated,and in the GelM A+hPTH(3-34)(29-34)group showed a greater increase than in the GelMA group(P＜0.05),while the expression of MMP9 and Cox2 was downregulated,and in the GelMA+hPTH(3-34)(29-34)group showed a greater decrease than in the GelMA group(P＜0.05).Compared with the control group,on Day 7 af-ter modeling,the relative activity of NLRP3,IL-6,TNF-α,IL-1β and Caspase-1 in rats decreased,and in the GelM A+hPTH(3-34)(29-34)group showed a greater decrease than in the GelMA group(P＜0.05).On Day 7 after modeling,the relative scratch distance of fibroblasts derived from the wound tissue in the GelMA+h PT H(3-34)(29-34)group was greater than that in the GelMA group,which was greater than that in the control group(P＜0.05).CONCLUSIONS The GelMA hydrogel loaded with hPTH(3-34)(29-34)short peptide can effectively accelerate the wound healing process and enhance tissue repair and structural reconstruction by inhibiting the activ-ity of the NLRP3 inflammasome pathway,demonstrating its potential to improve wound healing efficiency.

OBJECTIVE This study evaluates the role of Gelation metnacrylate(GelMA)hydrogel loaded with hPT H(3-34)(29-34)short peptide in the healing of chronic infectious skin wounds in diabetic rats.METHODS Ex-perimental rats were divided into control group,GelMA group and GelM A+hPTH(3-34)(29-34)group,with 15 rats in each group.Diabetic rat models were prepared,and chronic infectious skin wounds were constructed.Differences in wound area changes,wound healing-related indicator[type Ⅰ collagen α1 chain(COL1A1),α-smooth muscle actin(α-SMA),platelet endothelial cell adhesion molecule-1(CD31),matrix metalloproteinase-9(MMP9)and cyclooxygenase-2(Cox2)]expression and migration ability of fibroblasts in vitro were compared a-mong the groups.RESULTS Compared with the control group,the wound area of rats in the GelMA group de-creased on Day 7 and Day 14(P＜0.05),and the wound area of rats in the GelM A+hPTH(3-34)(29-34)group decreased on Day 3,Day 7 and Day 14(P＜0.05).Compared with the GelMA group,the wound area of rats in the GelM A+hPTH(3-34)(29-34)group decreased on Day 3,Day 7 and Day 14(P＜0.05).Compared with the control group,on Day 7 after modeling,the expression of COL1A1,α-SMA and CD31 in rats was upregulated,and in the GelM A+hPTH(3-34)(29-34)group showed a greater increase than in the GelMA group(P＜0.05),while the expression of MMP9 and Cox2 was downregulated,and in the GelMA+hPTH(3-34)(29-34)group showed a greater decrease than in the GelMA group(P＜0.05).Compared with the control group,on Day 7 af-ter modeling,the relative activity of NLRP3,IL-6,TNF-α,IL-1β and Caspase-1 in rats decreased,and in the GelM A+hPTH(3-34)(29-34)group showed a greater decrease than in the GelMA group(P＜0.05).On Day 7 after modeling,the relative scratch distance of fibroblasts derived from the wound tissue in the GelMA+h PT H(3-34)(29-34)group was greater than that in the GelMA group,which was greater than that in the control group(P＜0.05).CONCLUSIONS The GelMA hydrogel loaded with hPTH(3-34)(29-34)short peptide can effectively accelerate the wound healing process and enhance tissue repair and structural reconstruction by inhibiting the activ-ity of the NLRP3 inflammasome pathway,demonstrating its potential to improve wound healing efficiency.

Objective To compare the difference of dose distribution between inverse planning simulated annealing (IPSA) and hybrid inverse treatment planning and optimization (HIPO) in 3D brachytherapy plan of cervical cancer,and to provide evidence for selection of reverse planning optimization method for cervical cancer brachytherapy.Methods From Dec 2016 to May 2017,totally 43 cases of patients with cervical cancer radical surgery were selected.Original IPSA brachytherapy treatment plan optimization was applied to all cases.Based on the information of original image,IPSA and HIPO plans were established according to the same initial conditions.Parameters of Dg0,D100,V100％,Homogeneity Index (HI),and conformal index (CI) of the bladder,rectum and sigmoid D2 cm3 data for High-Risk Clinical Target Volume (HR-CTV) were assessed.Results There was no statistically significant difference in D90,D100 and CI for HR-CTV between the two groups.But the V100％ of HR-CTV in HIPO group was significantly higher than that in IPSA group [(87.72 ±0.49)％ vs.(85.01 ± 0.55)％,t =2.54,P ＜0.05].Furthermore,HI in HIPO group was (0.51 ±0.08),which was higher than that in IPSA group (0.42 ± 0.06),and the difference was statistically significant (t =3.02,P ＜ 0.05).Compared with IPSA,bladder D2 cm3 and rectum D2 cm3 [(3.04 ± 0.37) Gy] for HIPO plan were lower [(3.42 ± 0.17) Gy vs.(3.57 ± 0.28) Gy,(3.04 ± 0.37) Gy vs.(3.57 ± 0.28) Gy],which had reached statistical significance (t =0.27,0.19,P ＜ 0.05).There was no statistical significance in the D2 cm3 dose of sigmoid.Conclusions In the treatment of cervical cancer,better target area HI and less irradiated dose of bladder and rectum can be obtained by HIPO optimization than IPSA optimization.