Objectives:To evaluate the imaging effects of the novel sympathetic nerve imaging agent 18F-FPMBBG in healthy volunteers and heart failure patients.Methods:Four healthy volunteers and four heart failure patients were selected to undergo 18F-FPMBBG positron emission tomography/computed tomography(PET/CT)dynamic imaging,the radioactivity distribution characteristics of 18F-FPMBBG in the heart and adjacent organs of the two groups were observed,and the uptake of 18F-FPMBBG by the left ventricular myocardium was compared in the two groups.Results:No adverse effects were observed in all subjects after intravenous injection of 18F-FPMBBG.In healthy volunteers,the heart uptake was rapid and stable,lung uptake was very low,and the blood pool and liver clearance were fast.The heart/liver uptake ratios at 30,60,and 90 minutes after injection were 2.33±0.81,3.29±0.90 and 3.80±1.07,respectively.The average standard uptake value(SUVmean)of 18F-FPMBBG in the heart failure group was significantly lower than that in the healthy volunteer group(P=0.003).The washout rate(WR)was significantly higher in the heart failure group([16.53±2.76]%vs.[3.88±4.51]%,P=0.003).Conclusions:18F-FPMBBG showed good imaging and diagnostic effects in the preliminary imaging of healthy subjects and heart failure patients,and it has the potential to become an ideal cardiac sympathetic nerve imaging agent.

Objective:To explore the feasibility and clinical application value of deep inspiration breath-hold SPECT pulmonary perfusion imaging (DBQ).Methods:Twenty-eight patients (15 males, 13 females, age (38±18) years) who had pulmonary diseases and visited China-Japan Friendship Hospital between September 2023 and February 2024 were prospectively included, including 18 pulmonary embolism cases, 5 chronic obstructive pulmonary disease cases, and 5 pulmonary interstitial fibrosis cases. All patients underwent DBQ, traditional pulmonary perfusion imaging (Q), and low-dose CT (LDCT). The acquisition parameters of DBQ were determined through National Electrical Manufactures Association (NEMA) International Electrotechnical Commission (IEC) phantom model research. The radioactive counts, three-dimensional registration error, and heterogeneity of DBQ and Q were compared using paired t test, while the comparison of detection rates for pulmonary embolism lesions was performed using McNemar test. Results:The radioactive counts of DBQ and Q were (3.6±0.6)×10 6 and (4.5±0.7)×10 6( t=2.48, P=0.018), respectively. In 18 patients with pulmonary embolism, compared with Q-LDCT, DBQ-LDCT detected 8 more lesions, thus increasing the detection rare (41 vs 33; χ2=8.69, P=0.004). Among the 28 patients, the registration errors between DBQ and LDCT vs Q and LDCT were (3.1±0.4) vs (8.2±0.8)mm ( t=3.82, P<0.001), respectively. The heterogeneity of DBQ was significantly different from that of Q (0.74±0.15 vs 0.85±0.11; t=2.38, P=0.029). Conclusion:DBQ shows better image quality and diagnostic effect than traditional perfusion imaging.

Objective:To explore the feasibility and clinical application value of deep inspiration breath-hold SPECT pulmonary perfusion imaging (DBQ).Methods:Twenty-eight patients (15 males, 13 females, age (38±18) years) who had pulmonary diseases and visited China-Japan Friendship Hospital between September 2023 and February 2024 were prospectively included, including 18 pulmonary embolism cases, 5 chronic obstructive pulmonary disease cases, and 5 pulmonary interstitial fibrosis cases. All patients underwent DBQ, traditional pulmonary perfusion imaging (Q), and low-dose CT (LDCT). The acquisition parameters of DBQ were determined through National Electrical Manufactures Association (NEMA) International Electrotechnical Commission (IEC) phantom model research. The radioactive counts, three-dimensional registration error, and heterogeneity of DBQ and Q were compared using paired t test, while the comparison of detection rates for pulmonary embolism lesions was performed using McNemar test. Results:The radioactive counts of DBQ and Q were (3.6±0.6)×10 6 and (4.5±0.7)×10 6( t=2.48, P=0.018), respectively. In 18 patients with pulmonary embolism, compared with Q-LDCT, DBQ-LDCT detected 8 more lesions, thus increasing the detection rare (41 vs 33; χ2=8.69, P=0.004). Among the 28 patients, the registration errors between DBQ and LDCT vs Q and LDCT were (3.1±0.4) vs (8.2±0.8)mm ( t=3.82, P<0.001), respectively. The heterogeneity of DBQ was significantly different from that of Q (0.74±0.15 vs 0.85±0.11; t=2.38, P=0.029). Conclusion:DBQ shows better image quality and diagnostic effect than traditional perfusion imaging.

Objectives:To evaluate the imaging effects of the novel sympathetic nerve imaging agent 18F-FPMBBG in healthy volunteers and heart failure patients.Methods:Four healthy volunteers and four heart failure patients were selected to undergo 18F-FPMBBG positron emission tomography/computed tomography(PET/CT)dynamic imaging,the radioactivity distribution characteristics of 18F-FPMBBG in the heart and adjacent organs of the two groups were observed,and the uptake of 18F-FPMBBG by the left ventricular myocardium was compared in the two groups.Results:No adverse effects were observed in all subjects after intravenous injection of 18F-FPMBBG.In healthy volunteers,the heart uptake was rapid and stable,lung uptake was very low,and the blood pool and liver clearance were fast.The heart/liver uptake ratios at 30,60,and 90 minutes after injection were 2.33±0.81,3.29±0.90 and 3.80±1.07,respectively.The average standard uptake value(SUVmean)of 18F-FPMBBG in the heart failure group was significantly lower than that in the healthy volunteer group(P=0.003).The washout rate(WR)was significantly higher in the heart failure group([16.53±2.76]%vs.[3.88±4.51]%,P=0.003).Conclusions:18F-FPMBBG showed good imaging and diagnostic effects in the preliminary imaging of healthy subjects and heart failure patients,and it has the potential to become an ideal cardiac sympathetic nerve imaging agent.

Objective:To investigate the current application of blood purification in the treatment of acute poisoning within Jiangsu Province and to evaluate the impact of extracorporeal blood purification on the clinical outcomes of critically poisoned patients.Methods:This multicenter, cross-sectional real-world observational study followed patients presenting with poisoning to the emergency departments of nine hospitals in Jiangsu Province between June 2015 and May 2019. Data were collected on demographic characteristics, vital signs within the first hour of emergency presentation, treatment modalities, length of hospital stay, and survival outcomes. Clinical data from patients who underwent extracorporeal blood purification were compared with those who did not, using the Wilcoxon rank-sum test and Chi-square test.Results:A total of 4 178 poisoning cases were included between June 2015 and May 2019. Among them, 21.7% (908/4 178) received blood purification therapy, while 78.3% (3 270/4 178) did not. Hemoperfusion (90.4%) was the most frequently employed method, followed by continuous renal replacement therapy (CRRT) (4.4%). In combined blood purification modalities, 4.8% underwent hemoperfusion combined with CRRT, 0.1% received hemoperfusion with plasma exchange, and another 0.1% underwent hemoperfusion combined with both CRRT and plasma exchange. Among patients who underwent blood purification, pesticide poisoning was the most prevalent (76.3%), with the most common toxic agents being paraquat (23.7%), dichlorvos (8.7%), methamidophos (5.2%), omethoate (4.0%), and glyphosate (3.7%). Compared to the non-blood purification group, patients in the blood purification group were more likely to present within the first hour with a low Glasgow Coma Scale (GCS) score (3-8) (22.6% vs. 9.7%, P <0.05), low mean arterial pressure (8.0% vs. 3.2%, P <0.05), longer hospital stays [5(3,9) days vs. 2(1,4) days, P <0.05] and a higher in-hospital mortality rate (21.1% vs. 5.3%, P <0.05). Follow-up via telephone 28 days after discharge revealed a survival rate of 78.9%, with a mortality rate of 21.1% in the blood purification group. Conclusions:Hemoperfusion is the most commonly utilized blood purification technique for treating poisoning in Jiangsu Province, with pesticides being the primary toxic agents treated. Although the mortality rate is higher in the blood purification group, the intervention may still contribute to improved patient outcomes.

convalescents, and to screen for broad-spectrum neutralizing antibodies against the SARS-CoV-2 RBD. Methods Using biotinylated RBD as a molecular probe, flow cytometry was employed to perform single-cell sorting of B cells from peripheral blood mononuclear cells (PBMCs) of convalescents. The obtained B cells were lysed and subjected to reverse transcription, followed by nested PCR amplification of the heavy and light chains of antibodies was conducted using random primers. The amplified products were cloned into corresponding expression vectors, and the respective matched heavy-light chain plasmids were co-transfected into 293F cells for expression. Monoclonal antibodies were then purified using Protein A column chromatography. Neutralization experiments were conducted with the wild-type (WT) pseudovirus, and antibodies with IC50<0.1 μg/mL were selected for further testing of neutralizing breadth and potency against the wild-type (WT), Beta variant (B.1.351), Delta variant (B.1.617.2), and currently prevalent pseudovirus strains (XBB, BA.5, BF.7). Results A total of 21 RBD-specific monoclonal B cells were obtained from two recovered patients, resulting in the isolation of 13 pairs of antibody light/heavy chains. Nine antibodies were successfully expressed, with P1-A1, P1-B6, and P1-B9 exhibiting IC50 values below 0.1 μg/mL against the pseudovirus of the wild-type strain (WT). Specifically, P1-B6 effectively neutralized the wild-type strain (WT), Beta variant (B.1.351), and Delta variant (B.1.617.2), with IC50 values reaching 0.01 μg/mL. P1-B9 demonstrated effective neutralization against the wild-type strain (WT), Beta variant (B.1.351), Delta variant (B.1.617.2), and Gamma variant (P.1) pseudoviruses, with IC50 values of 0.42 μg/mL, 0.63 μg/mL, 0.28 μg/mL, and 2.50 μg/mL, respectively. Additionally, P1-B6 exhibited good neutralization against BA.5 and BF.7 pseudoviruses, with IC50 values of 0.06 μg/mL and 0.09 μg/mL, respectively. Conclusions Infection with the SARS-CoV-2 WT strain can induce the generation of neutralizing antibodies with broad-spectrum activity. Generating these broadly neutralizing antibodies does not require an excessively high somatic hypermutation. The obtained antibodies can be used as candidates for SARS-CoV-2 diagnosis and prevention.

Objective:To evaluate the performance of our self-developed deep learning (DL) model which was designed to automatically classify the knee X-ray images into one non-surgical category and 4 surgical categories, including non-surgical knees (NSK), high tibial osteotomy (HTO), total knee arthroplasty (TKA), unicompartmental knee arthroplasty (UKA), and tibial plateau fracture fixation with an internal fixation plate (TPFF).Methods:The knee X-ray images were collected of the patients who had undergone knee joint surgery at Department of Orthopaedics, Fuzhou Second General Hospital from January 2017 to December 2022. On the Baidu EasyDL AI platform, a multi-class object recognition DL model was built using the You Only Look Once (YOLO) algorithm. The model was trained on a dataset of 1,281 knee anteroposterior X-ray images (including NSK, HTO, TKA, UKA, and TPFF) to generate a DL model which was able to automatically recognize and classify the knee X-ray images. The reliability of the model classification performance was evaluated by analyzing the 5 indicators [accuracy, sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV)] using a test set of 450 knee anteroposterior X-ray images with the above 5 categories. The receiver operating characteristic curve was plotted and the area under the curve (AUC) was calculated to further quantify the classification performance of the model.Results:In the test set, on the whole, the model achieved an accuracy of 97.0%, a sensitivity of 92.4%, a specificity of 98.1%, a PPV of 92.4%, and a NPV of 98.1%, an AUC of 0.947, indicating a high reliability in classifying various categories. The model showed a best performance for TKA, with the 5 indicators being 99.1%, 99.0%, 99.1%, 97.1% and 99.7%. The model showed a slightly lower sensitivity for TPFF and HTO (87.0% and 86.0%, respectively).Conclusion:A successful DL model has been developed which can automatically classify the knee X-ray images into non-surgical and surgical categories due to its satisfactory performance, particularly in accuracy and AUC.

Renal foreign-body is rare in clinical practice. This article reports a case of 12-year-old male patient who presented with intermittent right lumbar stabbing pain for one year. Physical and laboratory examinations showed no significant abnormalities. Enhanced CT and three-dimensional angiography indicated a metal object in the anterior superior pole of the right kidney. The patient had a history of consuming vermicelli frequently over the past year. Considering the medical history and clinical examinations, it was hypothesized that a foreign body had penetrated the duodenum and lodged into the kidney. A foreign body extraction was performed via 3D laparoscopy, confirming the object to be a 25 mm long metallic needle. The patient was discharged on the third postoperative day. Follow-up at six months showed the patient to be in good condition.

Objective:To explore application effect of traceability management model of a quality control in the management of medical equipment in the department of burns and plastic surgery. Methods:The model architecture of software as a service (SAAS) was adopted,and the data layer,application layer,database operation abstraction layer,and client layer were established. The key traceability points for quality control were analyzed,and the traceability management model of quality control was constructed. A total of 50 commonly used medical equipment in clinical use in the department of burns and plastic surgery at the Second Affiliated Hospital of Air Force Medical University from November 2021 to October 2023 were selected. Equipment use management from November 2021 to October 2022 was conducted using the conventional management method (conventional management). Equipment use management from November 2022 to October 2023 was conducted using the traceability management model of quality control (quality traceability management). A self-made questionnaire was used to investigate the satisfaction of 60 personnel involved in the use,repair,and maintenance of equipment. The quality scores for equipment cleaning and disinfection management,and the evaluation scores for equipment management defects were compared between the two management methods. Results:The scores of equipment cleaning quality,sterilization quality,packaging quality and distribution quality of using the traceability management method of quality were (95.51±3.34),(96.82±4.08),(95.37±3.64) and (97.29±3.22),respectively,all of which were higher than those of the conventional management method,and the differences were statistically significant (t=12.024,9.549,12.156,14.414,P<0.05),respectively. The defect scores of the equipment using the traceability management method of quality were (0.34±0.01) points,(0.12±0.04) points and (0.46±0.14) points,respectively,which were lower than those of the conventional management method,and he differences were statistically significant (t=14.638,13.889,16.968,P<0.05). The satisfaction rate of the 60 managers involved in the use of the equipment was 98.33％(59/60),which was higher than that of the conventional management method,and the difference was statistically significant (x2=5.886,P<0.05). Conclusion:The application of the traceability management model of quality control to the management of medical equipment in the department of burn and plastic surgery can enhance the efficiency of equipment use and operator satisfaction,and improve the quality of equipment management.

Objective:To explore application effect of traceability management model of a quality control in the management of medical equipment in the department of burns and plastic surgery. Methods:The model architecture of software as a service (SAAS) was adopted,and the data layer,application layer,database operation abstraction layer,and client layer were established. The key traceability points for quality control were analyzed,and the traceability management model of quality control was constructed. A total of 50 commonly used medical equipment in clinical use in the department of burns and plastic surgery at the Second Affiliated Hospital of Air Force Medical University from November 2021 to October 2023 were selected. Equipment use management from November 2021 to October 2022 was conducted using the conventional management method (conventional management). Equipment use management from November 2022 to October 2023 was conducted using the traceability management model of quality control (quality traceability management). A self-made questionnaire was used to investigate the satisfaction of 60 personnel involved in the use,repair,and maintenance of equipment. The quality scores for equipment cleaning and disinfection management,and the evaluation scores for equipment management defects were compared between the two management methods. Results:The scores of equipment cleaning quality,sterilization quality,packaging quality and distribution quality of using the traceability management method of quality were (95.51±3.34),(96.82±4.08),(95.37±3.64) and (97.29±3.22),respectively,all of which were higher than those of the conventional management method,and the differences were statistically significant (t=12.024,9.549,12.156,14.414,P<0.05),respectively. The defect scores of the equipment using the traceability management method of quality were (0.34±0.01) points,(0.12±0.04) points and (0.46±0.14) points,respectively,which were lower than those of the conventional management method,and he differences were statistically significant (t=14.638,13.889,16.968,P<0.05). The satisfaction rate of the 60 managers involved in the use of the equipment was 98.33％(59/60),which was higher than that of the conventional management method,and the difference was statistically significant (x2=5.886,P<0.05). Conclusion:The application of the traceability management model of quality control to the management of medical equipment in the department of burn and plastic surgery can enhance the efficiency of equipment use and operator satisfaction,and improve the quality of equipment management.