Objective The oxidative damage of DNA can be caused by excessive levels of Reactive oxygen species(ROS).Monitoring of DNA oxidative damage enables effective evaluation of ROS damage effects.Although the detection of DNA damage effects based on microbial sensor allows quantitative analysis of oxidative damage,the ROS clearance mechanism existed in bacterial will affect the sensitive of detection.The work of this article is to knockout the key genes of ROS clearance mechanism and construct an antioxidant gene-knock-out microbial sensor.The microbial sensor can realize sensitive monitoring of DNA damage effects and then evaluates the damage effects of cells by ROS.Methods The antioxidant damage genes of bacterial ahpCF,katE and katG were knocked out by λ-Red homologous recombination and antioxidant gene-knockout microbial sensor was constructed.The nalidixic acid sodium salt and UV irradiation were used to characterize the performance for monitoring of DNA damage effects.Results The antioxidant gene-knockout microbial sensors ΔahpC,ΔahpCF/ΔkatEG and ΔahpCF/ΔkatE/ΔkatG were constructed successfully.The results showed that the microbial sensor ΔahpCF/ΔkatE/ΔkatGl had the highest sensitive of damage effects and the limit of detection for nalidixic acid sodium salt was 0.40 μmol/L.In addition,1.80 min of UV irradiation(254 nm)was sufficient to induce a significant fluorescent expression effect in the engineered bacteria.Conclusion In this article,antioxidant gene-knockout microbial sensors had been constructed to realize active and sensitive monitoring of DNA damage effects such as DNA damage reagents and UV irradiation.The sensors could provide an active,effective,and sensitive potential monitoring method for future evaluation of radiation effects in space.

Financial and accounting supervision has been incorporated into the national supervision system,giving it a new political position and a new positioning for supervision and governance work.With the continuous promotion of high-quality development in public hospitals,financial and accounting supervision needs to break through the"supervision on finance and accounting"situation and strengthen its governance attributes.It conducts literature review and analysis on the connotation,extension,and differentiation of financial and accounting supervision,as well as the construction of the financial and accounting supervision system in public hospitals.It explores the implementation and development of financial and accounting supervision in public hospitals,with the aim of providing guidance for financial and accounting supervision to fully leverage its diverse governance functions and further deepen the high-quality development of hospitals.

Financial and accounting supervision has been incorporated into the national supervision system,giving it a new political position and a new positioning for supervision and governance work.With the continuous promotion of high-quality development in public hospitals,financial and accounting supervision needs to break through the"supervision on finance and accounting"situation and strengthen its governance attributes.It conducts literature review and analysis on the connotation,extension,and differentiation of financial and accounting supervision,as well as the construction of the financial and accounting supervision system in public hospitals.It explores the implementation and development of financial and accounting supervision in public hospitals,with the aim of providing guidance for financial and accounting supervision to fully leverage its diverse governance functions and further deepen the high-quality development of hospitals.

Financial and accounting supervision has been incorporated into the national supervision system,giving it a new political position and a new positioning for supervision and governance work.With the continuous promotion of high-quality development in public hospitals,financial and accounting supervision needs to break through the"supervision on finance and accounting"situation and strengthen its governance attributes.It conducts literature review and analysis on the connotation,extension,and differentiation of financial and accounting supervision,as well as the construction of the financial and accounting supervision system in public hospitals.It explores the implementation and development of financial and accounting supervision in public hospitals,with the aim of providing guidance for financial and accounting supervision to fully leverage its diverse governance functions and further deepen the high-quality development of hospitals.

Financial and accounting supervision has been incorporated into the national supervision system,giving it a new political position and a new positioning for supervision and governance work.With the continuous promotion of high-quality development in public hospitals,financial and accounting supervision needs to break through the"supervision on finance and accounting"situation and strengthen its governance attributes.It conducts literature review and analysis on the connotation,extension,and differentiation of financial and accounting supervision,as well as the construction of the financial and accounting supervision system in public hospitals.It explores the implementation and development of financial and accounting supervision in public hospitals,with the aim of providing guidance for financial and accounting supervision to fully leverage its diverse governance functions and further deepen the high-quality development of hospitals.

[Objective] To explore the cellular composition and gene expression characteristics of adrenocortical adenoma (ACA) based on single-nuclear RNA sequencing. [Methods] The postoperative resection samples of 2 ACA cases treated at our hospital during Jul.and Aug.2022 were collected.The cellular composition was isolated and identified with single-nuclear RNA sequencing, the adrenal cortical subgroups were subdivided with specific cortical cell subgroup markers, and the characteristic gene expression profile was studied. [Results] The main components of ACA were adrenal cortical cells (78.62%), endothelial cells (1.91%), fibroblasts (3.09%), macrophages (14.34%), and T cells (2.05%). Subdivision of cortical cells revealed a group of undefined cells (1.08%), in addition to zona glomerulosa (ZG) cells (1.77%), zona fasciculata (ZF) cells (94.98%), and zona reticularis (ZR) cells (2.17%). These undefined cells were characterized by high expression of intercellular adhesion molecule 1, growth arrest and DNA damage inducing proteins β, tumor necrosis factor α inducing protein 3 and CD36 molecules.According to correlation analysis of the gene expression, these undefined cells were similar to the ZF cells.DEGs enrichment analysis indicated that the metabolic process enrichment index was the highest in biological processes.Reactome GO enrichment analysis revealed that the immune system cytokine signaling response was the most significant.KEGG signaling pathway analysis showed that Th17 cell differentiation pathway was the mostly enriched.DEGs were found to be most closely related to viral infection by DO enrichment analysis. [Conclusion] This study reveals for the first time the gene expression profile characteristics of cell subset in ACA, which can provide reference to explore the mechanism of ACA.

Financial and accounting supervision has been incorporated into the national supervision system,giving it a new political position and a new positioning for supervision and governance work.With the continuous promotion of high-quality development in public hospitals,financial and accounting supervision needs to break through the"supervision on finance and accounting"situation and strengthen its governance attributes.It conducts literature review and analysis on the connotation,extension,and differentiation of financial and accounting supervision,as well as the construction of the financial and accounting supervision system in public hospitals.It explores the implementation and development of financial and accounting supervision in public hospitals,with the aim of providing guidance for financial and accounting supervision to fully leverage its diverse governance functions and further deepen the high-quality development of hospitals.

Financial and accounting supervision has been incorporated into the national supervision system,giving it a new political position and a new positioning for supervision and governance work.With the continuous promotion of high-quality development in public hospitals,financial and accounting supervision needs to break through the"supervision on finance and accounting"situation and strengthen its governance attributes.It conducts literature review and analysis on the connotation,extension,and differentiation of financial and accounting supervision,as well as the construction of the financial and accounting supervision system in public hospitals.It explores the implementation and development of financial and accounting supervision in public hospitals,with the aim of providing guidance for financial and accounting supervision to fully leverage its diverse governance functions and further deepen the high-quality development of hospitals.

Financial and accounting supervision has been incorporated into the national supervision system,giving it a new political position and a new positioning for supervision and governance work.With the continuous promotion of high-quality development in public hospitals,financial and accounting supervision needs to break through the"supervision on finance and accounting"situation and strengthen its governance attributes.It conducts literature review and analysis on the connotation,extension,and differentiation of financial and accounting supervision,as well as the construction of the financial and accounting supervision system in public hospitals.It explores the implementation and development of financial and accounting supervision in public hospitals,with the aim of providing guidance for financial and accounting supervision to fully leverage its diverse governance functions and further deepen the high-quality development of hospitals.

Financial and accounting supervision has been incorporated into the national supervision system,giving it a new political position and a new positioning for supervision and governance work.With the continuous promotion of high-quality development in public hospitals,financial and accounting supervision needs to break through the"supervision on finance and accounting"situation and strengthen its governance attributes.It conducts literature review and analysis on the connotation,extension,and differentiation of financial and accounting supervision,as well as the construction of the financial and accounting supervision system in public hospitals.It explores the implementation and development of financial and accounting supervision in public hospitals,with the aim of providing guidance for financial and accounting supervision to fully leverage its diverse governance functions and further deepen the high-quality development of hospitals.