Objective: To investigate the potential protective effect of Shexiang Tongxin dropping pills (STDP) on ischemia-reperfusion injury and its underlying mechanisms in improving endothelial cell function in coronary microvascular disease (CMVD). Methods: A rat model of myocardial ischemia-reperfusion injury with CMVD was established using ligation and reperfusion of the left anterior descending artery. The effect of STDP (21.6 mg/kg) on cardiac function was evaluated using echocardiography, hematoxylin-eosin staining, and Evans blue staining. The effects of STDP on the microvascular endothelial barrier were assessed based on nitric oxide production, endothelial nitric oxide synthase expression, structural variety of tight junctions (TJs), and the expression of zonula occludens-1 (ZO-1), claudin-5, occludin, and vascular endothelial (VE)-cadherin proteins. The mechanisms of STDP (50 and 100 ng/mL) were evaluated by examining the expression of sphingosine 1-phosphate receptor 2 (S1PR2), Ras Homolog family member A (RhoA), and Rho-associated coiled-coil-containing protein kinase (ROCK) proteins and the distribution of ZO-1, VE-cadherin, and F-actin proteins in an oxygen and glucose deprivation/reoxygenation model. Results: The administration of STDP on CMVD rat model significantly improved cardiac and microvascular endothelial cell barrier functions (all P < .05). STDP enhanced the structural integrity of coronary microvascular positioning and distribution by clarifying and completing TJs and increasing the expression of ZO-1, occludin, claudin-5, and VE-cadherin in vivo (all P < .05). The S1PR2/RhoA/ROCK pathway was inhibited by STDP in vitro, leading to the regulation of endothelial cell TJs, adhesion junctions, and cytoskeletal morphology. Conclusion STDP showed protective effects on cardiac impairment and microvascular endothelial barrier injury in CMVD model rats induced by myocardial ischemia-reperfusion injury through the modulation of the S1PR2/RhoA/ROCK pathway.

Metastasis serves as an indicator of malignancy and is a biological characteristic of carcinomas. Epithelial-mesenchymal transition (EMT) plays a key role in the promotion of tumor invasion and metastasis and in the enhancement of tumor cell aggressiveness. Prostaglandin E synthase 3 (p23) is a cochaperone for heat shock protein 90 (HSP90). Our previous study showed that p23 is an HSP90-independent transcription factor in cancer-associated inflammation. The effect and mechanism of action of p23 on lung cancer metastasis are tested in this study. By utilizing cell models in vitro and mouse tail vein metastasis models in vivo, the results provide solid evidence that p23 is critical for promoting lung cancer metastases by regulating downstream CXCL1 expression. Rather than acting independently, p23 forms a complex with RNA-binding motif protein 14 (RBM14) to facilitate EMT progression in lung cancer. Therefore, our study provides evidence for the potential role of the RBM14-p23-CXCL1-EMT axis in the metastasis of lung cancer.

Financial and accounting supervision has been incorporated into the national supervision system,giving it a new political position and a new positioning for supervision and governance work.With the continuous promotion of high-quality development in public hospitals,financial and accounting supervision needs to break through the"supervision on finance and accounting"situation and strengthen its governance attributes.It conducts literature review and analysis on the connotation,extension,and differentiation of financial and accounting supervision,as well as the construction of the financial and accounting supervision system in public hospitals.It explores the implementation and development of financial and accounting supervision in public hospitals,with the aim of providing guidance for financial and accounting supervision to fully leverage its diverse governance functions and further deepen the high-quality development of hospitals.

Financial and accounting supervision has been incorporated into the national supervision system,giving it a new political position and a new positioning for supervision and governance work.With the continuous promotion of high-quality development in public hospitals,financial and accounting supervision needs to break through the"supervision on finance and accounting"situation and strengthen its governance attributes.It conducts literature review and analysis on the connotation,extension,and differentiation of financial and accounting supervision,as well as the construction of the financial and accounting supervision system in public hospitals.It explores the implementation and development of financial and accounting supervision in public hospitals,with the aim of providing guidance for financial and accounting supervision to fully leverage its diverse governance functions and further deepen the high-quality development of hospitals.

Financial and accounting supervision has been incorporated into the national supervision system,giving it a new political position and a new positioning for supervision and governance work.With the continuous promotion of high-quality development in public hospitals,financial and accounting supervision needs to break through the"supervision on finance and accounting"situation and strengthen its governance attributes.It conducts literature review and analysis on the connotation,extension,and differentiation of financial and accounting supervision,as well as the construction of the financial and accounting supervision system in public hospitals.It explores the implementation and development of financial and accounting supervision in public hospitals,with the aim of providing guidance for financial and accounting supervision to fully leverage its diverse governance functions and further deepen the high-quality development of hospitals.

Financial and accounting supervision has been incorporated into the national supervision system,giving it a new political position and a new positioning for supervision and governance work.With the continuous promotion of high-quality development in public hospitals,financial and accounting supervision needs to break through the"supervision on finance and accounting"situation and strengthen its governance attributes.It conducts literature review and analysis on the connotation,extension,and differentiation of financial and accounting supervision,as well as the construction of the financial and accounting supervision system in public hospitals.It explores the implementation and development of financial and accounting supervision in public hospitals,with the aim of providing guidance for financial and accounting supervision to fully leverage its diverse governance functions and further deepen the high-quality development of hospitals.

Financial and accounting supervision has been incorporated into the national supervision system,giving it a new political position and a new positioning for supervision and governance work.With the continuous promotion of high-quality development in public hospitals,financial and accounting supervision needs to break through the"supervision on finance and accounting"situation and strengthen its governance attributes.It conducts literature review and analysis on the connotation,extension,and differentiation of financial and accounting supervision,as well as the construction of the financial and accounting supervision system in public hospitals.It explores the implementation and development of financial and accounting supervision in public hospitals,with the aim of providing guidance for financial and accounting supervision to fully leverage its diverse governance functions and further deepen the high-quality development of hospitals.

Financial and accounting supervision has been incorporated into the national supervision system,giving it a new political position and a new positioning for supervision and governance work.With the continuous promotion of high-quality development in public hospitals,financial and accounting supervision needs to break through the"supervision on finance and accounting"situation and strengthen its governance attributes.It conducts literature review and analysis on the connotation,extension,and differentiation of financial and accounting supervision,as well as the construction of the financial and accounting supervision system in public hospitals.It explores the implementation and development of financial and accounting supervision in public hospitals,with the aim of providing guidance for financial and accounting supervision to fully leverage its diverse governance functions and further deepen the high-quality development of hospitals.

[Objective] To explore the cellular composition and gene expression characteristics of adrenocortical adenoma (ACA) based on single-nuclear RNA sequencing. [Methods] The postoperative resection samples of 2 ACA cases treated at our hospital during Jul.and Aug.2022 were collected.The cellular composition was isolated and identified with single-nuclear RNA sequencing, the adrenal cortical subgroups were subdivided with specific cortical cell subgroup markers, and the characteristic gene expression profile was studied. [Results] The main components of ACA were adrenal cortical cells (78.62%), endothelial cells (1.91%), fibroblasts (3.09%), macrophages (14.34%), and T cells (2.05%). Subdivision of cortical cells revealed a group of undefined cells (1.08%), in addition to zona glomerulosa (ZG) cells (1.77%), zona fasciculata (ZF) cells (94.98%), and zona reticularis (ZR) cells (2.17%). These undefined cells were characterized by high expression of intercellular adhesion molecule 1, growth arrest and DNA damage inducing proteins β, tumor necrosis factor α inducing protein 3 and CD36 molecules.According to correlation analysis of the gene expression, these undefined cells were similar to the ZF cells.DEGs enrichment analysis indicated that the metabolic process enrichment index was the highest in biological processes.Reactome GO enrichment analysis revealed that the immune system cytokine signaling response was the most significant.KEGG signaling pathway analysis showed that Th17 cell differentiation pathway was the mostly enriched.DEGs were found to be most closely related to viral infection by DO enrichment analysis. [Conclusion] This study reveals for the first time the gene expression profile characteristics of cell subset in ACA, which can provide reference to explore the mechanism of ACA.