Objective To investigate the antitumor effect of genetically engineered bacterial vesicles loaded with the photosensitizer Ce6.Methods Genetic engineering techniques were used to overexpress interferon α(IFNα)in Escherichia coli Nissle 1917(EcN),and the corresponding bacterial vesicles(BVs-IFNα)were extracted.The photosensitizer Ce6 was loaded onto the surface of the bacterial vesicles via ultrasonication to obtain BVs-IFNα@Ce6 nanoparticles.The BVs-IFNα@Ce6 complex was characterized using dynamic light scattering,nanoparticle tracking technology,confocal imaging,and ultraviolet absorption spectroscopy.The obtained BVs-IFNα@Ce6 nanoparticles were co-cultured with mouse melanoma cells(B16F10)and mouse bone marrow-derived dendritic cells(BMDC).The efficacy of photodynamic therapy was assessed using the CCK-8 method,and its ability to induce immune activation was verified using flow cytometry.Results The BVs-IFNα@Ce6 nanoparticles were successfully prepared with a stable particle size of around 180 nm.Under red light irradiation,BVs-IFNα@Ce6 exhibited significant cytotoxicity against B16F10 tumor cells and effectively promoted the expressions of immune-related molecules MHC-II,CD80/CD86 on the surface of BMDC cells,demonstrating its remarkable ability to induce immune activation.Conclusion The BVs-IFNα@Ce6 nanoparticles possess dual functions of enhancing photodynamic therapy efficacy and activating antitumor immunity,showing potential for clinical translation.

Objective To investigate the antitumor effect of genetically engineered bacterial vesicles loaded with the photosensitizer Ce6.Methods Genetic engineering techniques were used to overexpress interferon α(IFNα)in Escherichia coli Nissle 1917(EcN),and the corresponding bacterial vesicles(BVs-IFNα)were extracted.The photosensitizer Ce6 was loaded onto the surface of the bacterial vesicles via ultrasonication to obtain BVs-IFNα@Ce6 nanoparticles.The BVs-IFNα@Ce6 complex was characterized using dynamic light scattering,nanoparticle tracking technology,confocal imaging,and ultraviolet absorption spectroscopy.The obtained BVs-IFNα@Ce6 nanoparticles were co-cultured with mouse melanoma cells(B16F10)and mouse bone marrow-derived dendritic cells(BMDC).The efficacy of photodynamic therapy was assessed using the CCK-8 method,and its ability to induce immune activation was verified using flow cytometry.Results The BVs-IFNα@Ce6 nanoparticles were successfully prepared with a stable particle size of around 180 nm.Under red light irradiation,BVs-IFNα@Ce6 exhibited significant cytotoxicity against B16F10 tumor cells and effectively promoted the expressions of immune-related molecules MHC-II,CD80/CD86 on the surface of BMDC cells,demonstrating its remarkable ability to induce immune activation.Conclusion The BVs-IFNα@Ce6 nanoparticles possess dual functions of enhancing photodynamic therapy efficacy and activating antitumor immunity,showing potential for clinical translation.

Femoral neck fracture (FNF) in the elderly patients is currently a major health challenge worldwide, with excessive consumption of medical resources, high incidence of complications as well as suboptimal outcome and prognosis. Hip joint arthroplasty (HJA) has been the mainstream treatment for FNF in the elderly, but the conventional surgical approaches and techniques are still confronted with a series of bottlenecks such as dislocation, limp and limb length discrepancy. In recent years, direct anterior approach (DAA) for HJA (DAA-HJA) has been a major new choice in the field of joint replacement, which achieves improved clinical effectiveness of HJA in the treatment of elderly FNF, due to the fact that DAA approach involves the neuromuscular interface and accords with the idea of soft tissue retention and enhanced recovery after surgery. However, there is still a lack of unified understanding of standard technique and procedure of DAA-HJA in the treatment of elderly FNF. Therefore, relevant experts from the Hip Joint Group of Chinese Orthopedics Association of Chinese Medical Association, Youth Arthrology Group of Orthopedic Committee of PLA, Orthopedic Committee of Chongqing Medical Association, Branch of Orthopedic Surgeons of Chongqing Medical Doctor Association and Sport Medicine Committee of Chongqing Medical Association were organized to formulate the " Chinese expert consensus on the technical standard of direct anterior hip arthroplasty for elderly femoral neck fracture ( version 2023)" based on evidence-based medicine. This consensus mainly proposed 13 recommendations covering indications, surgical plans, prosthesis selections, surgical techniques and processes, and postoperative management of DAA-HJA in elderly patients with FNF, aiming to promote standardized, systematic and patient-specific diagnosis and treatment to improve the functional prognosis of the patients.

Objective:To observe the clinical efficacy and influencing factors of hyperbaric oxygen (HBO) in the treatment of spine and spinal cord injuries.Methods:A total of 150 patients with spinal cord injuries admitted to Qingyang People’s Hospital from May 2017 to May 2019 were randomly divided into hyperbaric oxygen (HBO) group and control group, with 75 cases in each group, according to random number table method. The patients in both groups were given basic conventional treatment, and HBO treatment was added to the patients in the HBO group. The clinical efficacy and Frankel grades of neurological function before and after treatment were observed. The data of age, injury site, injury reason, injury severity, starting time of the postoperative HBO treatment, and times of HBO treatment in the HBO group were collected and analyzed by Logistic regression analysis for studying the risk factors affecting HBO clinical efficacy.Results:The total effective rate of the HBO group (88.00%) was significantly higher than that of the control group (61.33%) ( P < 0.01); after treatment, the proportion of Grade A patients in the HBO group (2.67%) was significantly lower than that of the control group (13.33%), while the proportion of Grade E patients (28.00%) in the HBO group was significantly higher than that of the control group (12.00%), with statistically significant difference ( P < 0.05). The injury severity, the starting time of postoperative HBO treatment, and the times of HBO treatment were the influencing factors of the HBO clinical efficacy for the patients with spine and spinal cord injuries ( P<0.05 or P<0.01). Logistic regression analysis showed that the starting time of postoperative HBO treatment was the risk factor of the HBO clinical efficacy ( P < 0.05). Conclusion:HBO can achieve an obvious efficacy in treating spine and spinal cord injuries by significantly promoting the recovery of neurological function. The starting time of postoperative HBO treatment is a risk factor of the HBO treatment of spine and spinal cord injuries. It suggests that HBO treatment should be carried out within 8 hours after operation, which can be more conducive to the recovery of neurological function.

Objective:To observe the clinical efficacy and influencing factors of hyperbaric oxygen (HBO) in the treatment of spine and spinal cord injuries.Methods:A total of 150 patients with spinal cord injuries admitted to Qingyang People’s Hospital from May 2017 to May 2019 were randomly divided into hyperbaric oxygen (HBO) group and control group, with 75 cases in each group, according to random number table method. The patients in both groups were given basic conventional treatment, and HBO treatment was added to the patients in the HBO group. The clinical efficacy and Frankel grades of neurological function before and after treatment were observed. The data of age, injury site, injury reason, injury severity, starting time of the postoperative HBO treatment, and times of HBO treatment in the HBO group were collected and analyzed by Logistic regression analysis for studying the risk factors affecting HBO clinical efficacy.Results:The total effective rate of the HBO group (88.00%) was significantly higher than that of the control group (61.33%) ( P < 0.01); after treatment, the proportion of Grade A patients in the HBO group (2.67%) was significantly lower than that of the control group (13.33%), while the proportion of Grade E patients (28.00%) in the HBO group was significantly higher than that of the control group (12.00%), with statistically significant difference ( P < 0.05). The injury severity, the starting time of postoperative HBO treatment, and the times of HBO treatment were the influencing factors of the HBO clinical efficacy for the patients with spine and spinal cord injuries ( P<0.05 or P<0.01). Logistic regression analysis showed that the starting time of postoperative HBO treatment was the risk factor of the HBO clinical efficacy ( P < 0.05). Conclusion:HBO can achieve an obvious efficacy in treating spine and spinal cord injuries by significantly promoting the recovery of neurological function. The starting time of postoperative HBO treatment is a risk factor of the HBO treatment of spine and spinal cord injuries. It suggests that HBO treatment should be carried out within 8 hours after operation, which can be more conducive to the recovery of neurological function.

AIM To observe the changes of plasma neuropeptide Y (NPY) levels in patients with essential hypertension(EH) taking Compound Qishao Jiangya Tablets (Radixet Rhizoma Notoginseng, Radix Paeoniae Alba, Rhizoma Gastrodiae, etc) and to investigate the significance of NPY in the pathogenesis of hypertension and the relationship between NPY and platelet disnormal function. METHODS The plasma NPY concentrations of 60 patients with EH and 20 normal ones were determined by radioimmunoassay, at the same time detected platelet aggregation rate and fibrinogen were detected. RESULTS The levels of plasma NPY in patients with EH were higher than that in normal control group. The NPY levels, the platelet aggregation rate, fibrinogen in the three periods of hypertension (period Ⅰ,Ⅱ, Ⅲ) were all significantly different and were increased with the severity of hypertension. CONCLUSION NPY may participate in the pathophysiological course of hypertension, and the plasma NPY, the platelet aggregation rate of the patients with EH were correlative. The plasma NPY should be dynamscally monitored in order to determine the disease degree. During the treatment for EH attention should be taken to decrease the platelet aggregation rate.