OBJECTIVE To explore the subtypes of human papillomavirus(HPV)infection and expressions of ser-um microribonucleic acid(miR)-21,cyclooxygenase-2(COX-2)mRNA,human leukocyte antigen-G(HLA-G)and squamous cell carcinoma antigen(SCC-Ag)in the patients with high-grade cervical lesions.METHODS Totally 51 high-grade cervical lesions patients with HPV infection who were treated in Ganzhou Maternal and Child Health Hospital from Jan.2022 to Jan.2024 were randomly selected as the infection group,52 high-grade cervical lesions patients who did not have HPV infection were chosen as the no infection group,meanwhile,54 healthy people who received physical examination were assigned as the healthy group.The subtypes of HPV infections and the types of infections were observed.The levels of serum miR-21,COX-2 mRNA,HLA-G and SCC-Ag were com-pared among the groups.The values of the indexes in diagnosis of HPV infection in the patients with high-grade cervical lesions were analyzed.RESULTS Totally 75 case-times of HPV infections were detected in the infection group;the patients with HPV16 infection or HPV18 infection were dominant among the patients with single sub-type of HPV infection.The patients with mixed infections of HPV16,HPV52 and HPV58 were dominant among the patients with multiple subtypes of HPV infection.There were significant differences in the levels of serum miR-21,COX-2 mRNA,HLA-G and SCC-Ag among the infection group,the no infection group and the healthy group(P＜0.05);the levels of the four indexes of the infection group were higher than those of the no infection group and the healthy group(P＜0.05);the levels of the four indexes of the no infection group were higher than those of the healthy group(P＜0.05).The area under the curve(AUC)value of the joint detection of the four in-dexes was higher than that of the single detection in diagnosis of the HPV infection in the patients with high-grade cervical lesions(P＜0.05),the sensitivity of the joint detection was 92.16％,with the specificity 92.32％.CONCLUSIONS The HPV genotype HPV16 is most common among the high-grade cervical lesions patients with HPV infection and is dominant among the patients with single and multiple subtypes of HPV infections.The miR-21,COX-2 mRNA,HLA-G and SCC-A are associated with the high-grade cervical lesions and the high-grade cer-vical lesions with HPV infection.The patients show high expressions of the four indexes.The joint detection of the four indexes has high value in diagnosis of the HPV infection in the patients with high-grade cervical lesions.

In 2022， Hainan provincial government launched the project for the prevention and control of viral hepatitis with the goals of a hepatitis B screening rate of 90%， a diagnostic rate of 90%， and a treatment rate of 80% among people aged 18 years and above by the year 2025， and the main intervention measures include population-based prevention， case screening， antiviral therapy， and health management. As of December 31， 2024， a total of 6.875 million individuals in the general population had been screened for hepatitis B， with a screening rate of 95.6%. A total of 184 710 individuals with positive HBsAg were identified， among whom 156 772 were diagnosed through serological reexamination， resulting in a diagnostic rate of 84.9%. A total of 50 742 patients with chronic hepatitis B were identified， among whom 42 921 had hepatitis B-specific health records established for health management， with a file establishment rate of 84.6%. A total of 31 553 individuals received antiviral therapy， with a treatment rate of 62.2%. A total of 2.503 million individuals at a high risk of hepatitis C were screened， among whom 4 870 tested positive for HCV antibody and 3 858 underwent HCV RNA testing， resulting in a diagnostic rate of 79.2%， and 1 824 individuals with positive HCV RNA were identified， among whom 1 194 received antiviral therapy， with a treatment rate of 65.5%. In addition， 159 301 individuals with negative HBsAg and anti-HBs and an age of 20 — 40 years were inoculated with hepatitis B vaccine free of charge. Through the implementation of the project for the prevention and control of viral hepatitis， a large number of hepatitis patients have been identified， treated， and managed in the province within a short period of time， which significantly accelerates the efforts to eliminate the crisis of viral hepatitis.

OBJECTIVE To explore the subtypes of human papillomavirus(HPV)infection and expressions of ser-um microribonucleic acid(miR)-21,cyclooxygenase-2(COX-2)mRNA,human leukocyte antigen-G(HLA-G)and squamous cell carcinoma antigen(SCC-Ag)in the patients with high-grade cervical lesions.METHODS Totally 51 high-grade cervical lesions patients with HPV infection who were treated in Ganzhou Maternal and Child Health Hospital from Jan.2022 to Jan.2024 were randomly selected as the infection group,52 high-grade cervical lesions patients who did not have HPV infection were chosen as the no infection group,meanwhile,54 healthy people who received physical examination were assigned as the healthy group.The subtypes of HPV infections and the types of infections were observed.The levels of serum miR-21,COX-2 mRNA,HLA-G and SCC-Ag were com-pared among the groups.The values of the indexes in diagnosis of HPV infection in the patients with high-grade cervical lesions were analyzed.RESULTS Totally 75 case-times of HPV infections were detected in the infection group;the patients with HPV16 infection or HPV18 infection were dominant among the patients with single sub-type of HPV infection.The patients with mixed infections of HPV16,HPV52 and HPV58 were dominant among the patients with multiple subtypes of HPV infection.There were significant differences in the levels of serum miR-21,COX-2 mRNA,HLA-G and SCC-Ag among the infection group,the no infection group and the healthy group(P＜0.05);the levels of the four indexes of the infection group were higher than those of the no infection group and the healthy group(P＜0.05);the levels of the four indexes of the no infection group were higher than those of the healthy group(P＜0.05).The area under the curve(AUC)value of the joint detection of the four in-dexes was higher than that of the single detection in diagnosis of the HPV infection in the patients with high-grade cervical lesions(P＜0.05),the sensitivity of the joint detection was 92.16％,with the specificity 92.32％.CONCLUSIONS The HPV genotype HPV16 is most common among the high-grade cervical lesions patients with HPV infection and is dominant among the patients with single and multiple subtypes of HPV infections.The miR-21,COX-2 mRNA,HLA-G and SCC-A are associated with the high-grade cervical lesions and the high-grade cer-vical lesions with HPV infection.The patients show high expressions of the four indexes.The joint detection of the four indexes has high value in diagnosis of the HPV infection in the patients with high-grade cervical lesions.

Objective:To assess the prognostic impact of frailty on elderly inpatients with heart failure.Methods:This prospective cohort study enrolled 121 in elderly patients with heart failure from Beijing Hospital, the General Hospital of the People's Liberation Army, and Beijing Tsinghua Changgung Hospital between September 2018 and April 2019.Patients were assessed for frailty using the Fried frailty phenotype and categorized into frail and non-frail groups.Follow-ups were conducted at 3-, 6-, and 12-months post-enrollment through clinic visits or phone calls to record adverse events.Composite endpoints include all-cause mortality and rehospitalization duo to deterioration of heart failure.Results:The study included 121 patients with an average age of 78.0±7.4 years, of whom 71(58.7%)were male and 57(47.1%)were classified as frail.Compared to the non-frail group, the frail group had lower estimated glomerular filtration rates[49.5±20.7 ml/(min·1.73m 2) vs.(64.0±27.1)ml/(min·1.73m 2)], lower scores in Basic Activities of Daily Living[5.0(4.0, 6.0) vs.6.0(5.0, 6.0)], Instrumental Activities of Daily Living[2.0(1.3, 7.8) vs.7.0(5.0, 8.0)], and Mini-Mental State Examination[26.0(16.0, 28.0) vs.27.0(22.3, 29.0)], all P<0.05.They also experienced longer hospital stays[10.5(6.0, 18.8)days vs.8.0(6.0, 11.8)days, P=0.008].During the follow-up period, the incidence of composite endpoint events was significantly higher in the frail group(43.9% vs.25.0%, P=0.029).Kaplan-Meier survival analysis demonstrated that the one-year incidence of composite endpoint events was significantly higher in the frail group( P=0.013).Multivariable Cox regression analysisindicated that frailty was an independent risk factor for composite endpoint events( HR=2.201, 95% CI: 1.089-4.447, P=0.028). Conclusions:Frailty is an independent risk factor for poor outcomes in elderly hospitalized patients with heart failure and should be considered a crucial factor in clinical assessment and treatment strategies.

Objective:To study the correlation between the acute-phase characteristics of motor evoked potential (MEP) and severities of spinal cord injury in patients with acute cervical hyperextension injury and central cord syndrome (CCS).Methods:Retrospectively analyzed were the data of 45 patients with acute cervical hyperextension injury and CCS (observation group) who had been admitted to Department of Orthopedics, Tongji Hospital Affiliated to Tongji University from December 2018 to July 2021 and 20 healthy controls. Examination of transcranial magnetic stimulation-induced MEP was performed in patients with CCS and healthy controls using a magpro x100 magnetic stimulator, and recording was conducted in bilateral abductor pollicis brevis (APB). The characteristics of MEP waveform latency, amplitude and motor threshold were described and compared between the healthy control and observation groups; the correlations were analyzed between the MEP latency and the severity of spinal cord injury [American Spinal Injury Association (ASIA) total score and motor function of Upper Extremity Motor Subscores (UEMS)] in the observation group. According to different MEP-induced states, the patients in the observation group were divided into a resting group ( n=19), a facilitation group ( n=18), and a no-waveform group ( n=8). The severity of spinal cord injury (ASIA total score) and the functional independence of the spinal cord (SCIM-Ⅲ score) were compared among the 3 groups to analyze the correlation between the MEP-induced state and the severity of spinal cord injury (ASIA total score). Results:The observation group had a significantly longer MEP latency [(30.16±6.32) ms], a significantly smaller amplitude [(0.54±0.30) mV] and a significantly higher motor threshold [(65%±11%)] than the healthy control group (all P<0.05). The MEP latency in the observation group was significantly correlated with ASIA total score ( r=-0.730, P<0.001) and UEMS ( r=-0.740, P<0.001). The ASIA total score and SCIM-Ⅲ score were significantly different among the 3 groups ( P<0.05), and the MEP-induced state was significantly correlated with the severity of spinal cord injury (ASIA total score) ( r=0.668, P<0.001). Conclusions:In patients with acute cervical hyperextension injury and CCS, the MEP latency is prolonged, the amplitude lowered, and the motor threshold enhanced. The MEP latency is strongly correlated with the severity of spinal cord injury and upper limb motor function. The MEP-induced state is also closely related to the severity of spinal cord injury.

Leucine rich glioma inactivated 1 (LGI1) is a protein which is identified as the target involving in autoimmune encephalitis. Seizures and cognitive declines are two main symptoms of LGI1-antibody encephalitis. However, autonomic dysfunction symptoms are not prominent as seizures and cognitive defection and are easily overlooked by physicians. We reported a case with LGI1-antibody encephalitis whose onset symptoms were autonomic dysfunction including sweating, orthostatic hypotension. The features of this case was described in detail and the related literatures were reviewed in order to enhance the knowledge of the disease.

Objective: To investigate the cumulative and sensitive period effects of family adversity on the outcome of psychopathological symptoms,so as to provide evidence for the prevention and intervention of adverse events. Methods: A total of 710 adolescents were recruited from local schools in rural area of Fuyang,Anhui Province in Dec. 2017 by using the convenience sampling method. The Adverse Childhood Experiences Questionnaire was used to assess family adversity. The MacArthur Health & Behavior Questionnaire was used to assess internalizing and externalizing symptoms. Multiple linear regression model was used to analyze the association between number and time of family adversity and psychopathological symptoms. Results: Persistent family adversity was associated with increased internalizing symptoms [(β(95%CI)=0.35(0.15-0.54)] and increased externalizing symptoms [β(95%CI)=0.16(0.01-0.32)]. 2 and ≥3 family adversities were associated with increased internalizing symptoms[β(95%CI)=0.20(0.04-0.36),0.42(0.24-0.60)]and increased externalizing symptoms[β(95%CI)=0.14(0.01-0.26),0.23(0.09-0.37)]. In childhood family adversity group,2 and ≥3 family adversities were associated with increased internalizing symptoms [β(95%CI)=0.23(0.06-0.41),0.34(0.11-0.58)] and increased externalizing symptoms [β(95%CI)=0.17(0.02-0.31),0.21(0.02-0.39)]. In persistent family adversity group,≥3 family adversities were associated with increased internalizing symptoms[β(95%CI)=0.56(0.31-0.82)] and increased externalizing symptoms [β(95%CI)=0.24(0.02-0.45)]. Adolescence family adversity was not associated with psychopathological symptoms. Conclusion The cumulative family adversity may increase the risk of psychopathological symptoms,and that childhood may be the sensitive period for family adversity to cause psychopathological symptoms.

Objective: To explore the correlation of health literacy and mobile phone use dependence with psychopathological symptoms in middle school students. Methods: 22 628 middle school students in Shenyang, Bengbu, Xinxiang, Ulanqab, Chongqing and Yangjiang were enrolled by multistage cluster sampling method from November 2015 to January 2016. Chinese Adolescent Interactive Health Literacy Questionnaire (CAIHLQ), Self-rating Questionnaire for Adolescent Problematic Mobile Phone Use (SQAPMPU) and Multidimensional Sub-health Questionnaire of Adolescents (MSQA) were applied to acquire basic characteristics, health literacy, mobile phone use dependence and psychopathological symptoms of subjects. Subjects were classified into three groups, low level (<P₂₅), medium level (P₂₅-P₇₅) and high level (>P₇₅), according to the percentile of the questionnaire score. Multivariate logistic regression model was used to analyze the correlation of health literacy, mobile phone with psychopathological symptoms. Results: The students were (15.4±1.8) years old with 10 990 boys (48.6%). The score of health literacy of students were (104.1±18.7) points. The rate of mobile phone use dependence was 25.4% (5 752/22 628) and the rate of psychopathological symptoms was 29.1% (6 581/22 628). Compared with high health literacy level, medium and low health literacy levels were related to psychopathological symptoms, with OR (95%CI) about 2.30 (2.10-2.52) and 5.40 (4.89-5.97), respectively. Compared with mobile phone use independence, mobile phone use dependence was related to psychopathological symptoms, with OR (95%CI) about 3.60(3.37-3.85). The highest rate of psychopathological symptoms occurred in students with mobile phone use dependence and low health literacy level [68.0% (1 345/1 977)], with OR (95%CI) about 19.59 (17.07-22.48). Conclusion Health literacy and mobile phone use dependence are related factors of psychopathological symptoms in middle school students.

Objective: To explore the association between co-consumption of takeaway fast foods and sodas with depressive symptoms among Chinese adolescents, and to provide a reference for reducing the occurence of depression among adolescents. Methods: A multi-center population-based survey was conducted in 32 schools in 4 provinces across China. A total of 14 500 adolescents completed eligible questionnaires. Depressive symptoms were assessed by using Children’s Depression Inventory(CDI), while consumption of takeaway fast foods and sodas was collected using the semi-quantitative Food Frequency Questionnaire(FFQ). Results: 27.26%(3 952/14 500) of adolescents were reported of depressive symptoms. The low, middle, and high-frequency consumption of takeaway fast foods (a OR=1.12, 1.73, 1.56, P<0.05) and sodas (a OR=1.64, 2.17, 3.54, P<0.01) were associated with depressive symptoms, and dose-response relationships were observed in all association(P<0.01). Meanwhile, positive additive interactions were observed in the association(a OR=2.46, P<0.01). The relative excess risk, attribution ratio and the interaction index of synergy was 0.45(0.12-0.77), 0.18(0.06-0.30) and 1.44(1.10-1.89), respectively. Conclusion Co-consumption of takeaway fast foods and sodas significantly associates with depressive symptoms by synergistic effect among Chinese adolescents.

Objective: The present study aimed to determine the prevalence of childhood abuse and examined its correlation with inflammatory factor IL-6 level in middle schools students. Methods: A total of 911 junior and high school students from a middle school in Shenyang were enrolled in this study to investigate the experience of childhood abuse and its association with IL-6 level in fasting blood samples in December 2017. Results: The prevalence of childhood maltreatment was 21.0%, the prevalence of physical, emotional and sexual abuse was 21.8%, 20.3% and 9.5%, respectively. Physical abuse, emotional abuse and total abuse were associated with high levels of IL-6 in junior high school boys, χ2 values were 3.88, 6.78, and 9.10, respectively (P<0.05). There was no significant correlation between abuse experiences with IL-6 levels among junior high school girls and senior high school students. Regression analysis showed that physical abuse, emotional abuse and total abuse were positively associated with IL-6 concentration among junior high school boys(OR=2.23, 3.49, 1.58, P<0.05). Conclusion Physical and emotional abuse in childhood associates with the increase of IL-6 level among junior school boys. Abnormal inflammatory factor level might be potential mechanism linking childhood abuse with adverse health outcomes.