Objective To investigate the correlation between peripheral blood CD14+CD16+monocytes and IgG N-glycosyl levels and disease activity in patients with systemic lupus erythematosus(SLE).Methods A total of 109 SLE patients admitted to Xingtai Cental Hospital from August 2021 to November 2024 were retrospectively selected as the study objects.According to SLE disease activity index(SLE-DAI),the patients were divided into active group(n=52)and stable group(n=57).In addition,56 patients who underwent physical examination during the same period were selected as the control group.Clinical data of patients were collected,CD14+CD16+mononuclear cells were detected by flow cytometry(FCM),and IgG N-glycosyl levels were detected by hydrophilic interaction chromatography-mass spectrometry.Logistic regression analysis was performed to analyze the influencing factors of SLE-DAI,and multicollinearity test[variance inflation factor(VIF)]was performed for independent variables.The prediction model of disease activity was constructed.The effectiveness of the predictive model was evaluated by describing the recviver operator characteristic(ROC)curve and calculating the area under the curve(AUC)value.Hosmer-Lemeshow goodness-of-fit test predicted the calibration degree of the model.Results The levels of WBC,Hb,PLT,ALB,complement C3 and complement C4 in control group were higher than those in SLE group(t=8.917～22.171),and the levels of CRP were lower than those in SLE group(t=-17.359),with differences were statistical significance(all P<0.05).The CRP level and the proportion of CD14+CD16+mononuclear cells in the active group were higher than those in the stable group,and the differences were statistically significant(t=5.449,11.112,all P<0.05).The IgG glycosylation characteristics of galactosylation,sialylation and N-acetylglucosamine modification were lower than those in the stable group,and the differences were statistical significance(Z=-2.432～-0.158,all P<0.05).Spearman correlation analysis showed that the proportion of CD14+CD16+monocytes was significantly negatively correlated with IgG galactosylation,sialylation level and bisection N-acetylglucosamine modification(r=-0.656,-0.531,-0.608,all P<0.01).CD14+CD16+monocyte ratio was positively correlated with SLE-DIA(r=0.581,all P<0.01).IgG galactosylation,sialylation levels and bisection N-acetylglucosamine modification were negatively correlated with SLE-DIA(r=-0.645,-0.609,-0.503,all P<0.01).Logistic regression analysis showed that CRP>8.21mg/L,CD14+CD16+≥16.17%,sialylation<22.05%and isotropic N-acetylglucosamine modification<16.53%were independent risk factors for disease activity in SLE patients(Wald χ2=4.471～12.811,all P<0.05).The VIF values of the above independent variables were all less than 10.By establishing the Logistic regression prediction model and drawing the ROC curve,the AUC value for diagnosing SLE disease activity was 0.821(95%CI:0.733～0.905),the sensitivity,specificity and the Yodon index were 85.37%,75.67%,0.677,respectively.and the P values of Hosmer-Lemeshow goodness-of-fit test models were 0.568,respectively.Conclusion The proportion of CD14+CD16+monocytes in peripheral blood of SLE patients increase significantly,and the level of IgG glycosylation characteristics decrease,both of which are correlated with SLE-DIA.The predictive model constructed based on the two had a good ability to distinguish SLE-DIA from inactive state,with high sensitivity and moderate specificity conducive to early clinical recognition,and the model fitting effect is good.SLE-DIA can be evaluated more accurately.

Objective To develop a model for visualizing the risk of hemorrhagic transformation(HT)in acute ischemic stroke(AIS)patients after alteplase thrombolysis based on routine labo-ratory data.Methods A total of 252 AIS patients receiving alteplase thrombolysis in our hospital from January 2021 to January 2024 were enrolled,and then divided into HT group and non-HT group according to developing HT or not within 24 h after thrombolysis.The influencing factors for HT in AIS patients after alteplase thrombolysis were analyzed,and then a model of visualizing the risk was conducted and its predictive performance was verified.Results HT occurred in 52 out of 252 AIS patients(20.63％).The HT group had significantly higher ratio of hypertension,higher NIHSS score at admission,elevated neutrophil count,and increased D-dimer(D-D)and to-tal bilirubin(TBIL)levels,whereas lower uric acid(UA)and serum potassium levels when com-pared with the non-HT group(P＜0.05,P＜0.01).Multivariate logistic regression analysis identi-fied neutrophils,D-D,and TBIL as independent risk factors for HT(OR=2.753,95％CI:1.399-5.417,P=0.003;OR=1.987,95％CI:1.322-2.986,P=0.001;OR=2.121,95％CI:1.392-3.230,P=0.000),while UA and serum potassium were protective factors(OR=0.417,95％CI:0.202-0.860,P=0.027;OR=0.160,95％CI:0.028-0.911,P=0.039).The AUC value of the constructed model in predicting HT in AIS patients after alteplase thrombolysis was 0.920(95％CI:0.880-0.950),with a sensitivity of 96.15％and a specificity of 80.50％.Hosmer-Lemeshow test(x2=1.888,P=0.169)confirmed the model had good fit,and Bootstrap internal validation yielded a C-index of 0.921.Conclusion Neutrophils,D-D,and TBIL are risk factors,while UA and serum potassium are protective factors for HT in AIS patients following alteplase thrombolysis.Our developed model of risk visualization demonstrates robust predictive performance.

Objective To develop a model for visualizing the risk of hemorrhagic transformation(HT)in acute ischemic stroke(AIS)patients after alteplase thrombolysis based on routine labo-ratory data.Methods A total of 252 AIS patients receiving alteplase thrombolysis in our hospital from January 2021 to January 2024 were enrolled,and then divided into HT group and non-HT group according to developing HT or not within 24 h after thrombolysis.The influencing factors for HT in AIS patients after alteplase thrombolysis were analyzed,and then a model of visualizing the risk was conducted and its predictive performance was verified.Results HT occurred in 52 out of 252 AIS patients(20.63％).The HT group had significantly higher ratio of hypertension,higher NIHSS score at admission,elevated neutrophil count,and increased D-dimer(D-D)and to-tal bilirubin(TBIL)levels,whereas lower uric acid(UA)and serum potassium levels when com-pared with the non-HT group(P＜0.05,P＜0.01).Multivariate logistic regression analysis identi-fied neutrophils,D-D,and TBIL as independent risk factors for HT(OR=2.753,95％CI:1.399-5.417,P=0.003;OR=1.987,95％CI:1.322-2.986,P=0.001;OR=2.121,95％CI:1.392-3.230,P=0.000),while UA and serum potassium were protective factors(OR=0.417,95％CI:0.202-0.860,P=0.027;OR=0.160,95％CI:0.028-0.911,P=0.039).The AUC value of the constructed model in predicting HT in AIS patients after alteplase thrombolysis was 0.920(95％CI:0.880-0.950),with a sensitivity of 96.15％and a specificity of 80.50％.Hosmer-Lemeshow test(x2=1.888,P=0.169)confirmed the model had good fit,and Bootstrap internal validation yielded a C-index of 0.921.Conclusion Neutrophils,D-D,and TBIL are risk factors,while UA and serum potassium are protective factors for HT in AIS patients following alteplase thrombolysis.Our developed model of risk visualization demonstrates robust predictive performance.

Objective To investigate the correlation between peripheral blood CD14+CD16+monocytes and IgG N-glycosyl levels and disease activity in patients with systemic lupus erythematosus(SLE).Methods A total of 109 SLE patients admitted to Xingtai Cental Hospital from August 2021 to November 2024 were retrospectively selected as the study objects.According to SLE disease activity index(SLE-DAI),the patients were divided into active group(n=52)and stable group(n=57).In addition,56 patients who underwent physical examination during the same period were selected as the control group.Clinical data of patients were collected,CD14+CD16+mononuclear cells were detected by flow cytometry(FCM),and IgG N-glycosyl levels were detected by hydrophilic interaction chromatography-mass spectrometry.Logistic regression analysis was performed to analyze the influencing factors of SLE-DAI,and multicollinearity test[variance inflation factor(VIF)]was performed for independent variables.The prediction model of disease activity was constructed.The effectiveness of the predictive model was evaluated by describing the recviver operator characteristic(ROC)curve and calculating the area under the curve(AUC)value.Hosmer-Lemeshow goodness-of-fit test predicted the calibration degree of the model.Results The levels of WBC,Hb,PLT,ALB,complement C3 and complement C4 in control group were higher than those in SLE group(t=8.917～22.171),and the levels of CRP were lower than those in SLE group(t=-17.359),with differences were statistical significance(all P<0.05).The CRP level and the proportion of CD14+CD16+mononuclear cells in the active group were higher than those in the stable group,and the differences were statistically significant(t=5.449,11.112,all P<0.05).The IgG glycosylation characteristics of galactosylation,sialylation and N-acetylglucosamine modification were lower than those in the stable group,and the differences were statistical significance(Z=-2.432～-0.158,all P<0.05).Spearman correlation analysis showed that the proportion of CD14+CD16+monocytes was significantly negatively correlated with IgG galactosylation,sialylation level and bisection N-acetylglucosamine modification(r=-0.656,-0.531,-0.608,all P<0.01).CD14+CD16+monocyte ratio was positively correlated with SLE-DIA(r=0.581,all P<0.01).IgG galactosylation,sialylation levels and bisection N-acetylglucosamine modification were negatively correlated with SLE-DIA(r=-0.645,-0.609,-0.503,all P<0.01).Logistic regression analysis showed that CRP>8.21mg/L,CD14+CD16+≥16.17%,sialylation<22.05%and isotropic N-acetylglucosamine modification<16.53%were independent risk factors for disease activity in SLE patients(Wald χ2=4.471～12.811,all P<0.05).The VIF values of the above independent variables were all less than 10.By establishing the Logistic regression prediction model and drawing the ROC curve,the AUC value for diagnosing SLE disease activity was 0.821(95%CI:0.733～0.905),the sensitivity,specificity and the Yodon index were 85.37%,75.67%,0.677,respectively.and the P values of Hosmer-Lemeshow goodness-of-fit test models were 0.568,respectively.Conclusion The proportion of CD14+CD16+monocytes in peripheral blood of SLE patients increase significantly,and the level of IgG glycosylation characteristics decrease,both of which are correlated with SLE-DIA.The predictive model constructed based on the two had a good ability to distinguish SLE-DIA from inactive state,with high sensitivity and moderate specificity conducive to early clinical recognition,and the model fitting effect is good.SLE-DIA can be evaluated more accurately.

Objective:To explore the clinical application of a new type of medical drainage device designed to measure the intra-abdominal pressure of ICU patients.Methods:The 65 patients with severe acute pancreatitis treated in our hospital from April to September 2018 were selected as the experimental group; the 54 patients with severe acute pancreatitis treated from October 2017 to March 2018 were selected as the control group. Patients in the control group used traditional drainage bags to measure intra-abdominal pressure, while patients in the experimental group used a new-designed medical drainage device to measure intra-abdominal pressure. Compare the cost of consumables used for the first time in the experimental group with the control group, the incidence of acupuncture injuries, the incidence of urethral leakage, the incidence of rupture of the catheter balloon, and the satisfaction of the nurses. The 32 patients admitted from January to March 2018 in the control group were measured again using a new drainage device after the abdominal pressure measurement operation was stopped in order to compare the accuracy of the two methods.Results:The per capita consumable cost of the experimental group was 5.71 yuan, 0 cases of needle stick injury, 0 case of catheter leakage, which were lower than the control group (22.36 yuan, 1 case, 7 cases), the difference was significant ( P<0.05). The nurse operation satisfaction score was 13.85±0.93, which was higher than the control group (10.00±1.05). The difference was statistically significant ( t value was -20.323, P<0.05). Conclusion:In the operation of intra-abdominal pressure measurement, the use of a new type of medical drainage device can ensure the accuracy of the measurement, reduce the cost of consumables, needle stick injuries and the incidence of urinary catheter leakage, and improve nurse operation satisfaction.

OBJECTIVETo observe the protective effect of combined administration of blood-activating drug and sedative drug on myocardial injury of acute myocardial infarction (AMI) rats.

METHODThe acute myocardial infarction (AMI) model was established by occluding the left descending coronary artery of Wistar rats. These rats were further divided into four groups (n = 15 per group): the sham-operated group, the AMI model group, the blood-activating drug group and the combined administration group.

RESULTCompared with the sham-operated group, the AMI model group showed significant decrease in ejection fraction (EF) and fractional shortening (FS) (P < 0.001) and notable increase in the left ventricular end-diastolic internal diameter (LVIDd) and left ventricular end-systolic internal diameter (LVIDs) (P < 0.01), with the infarct area of left ventricular front wall up to about 70%-90%. Besides, tissue was severely replaced by collagen deposition and fibrosis, the sarcomeres disorganized and mitochondrial abnormalized. Compared with the AMI model group, the blood-activating drug group and the combined administration group showed significant increase in the values of EF and FS (P < 0.05 or P < 0.01) and obvious reduction in LVIDd and LVIDs (P < 0.05 or P < 0.01), with the infarction area of left ventricular front wall up to about 40%-60%. The collagen deposition and myocardium fibrosis, the disorganized sarcomeres and mitochondrial abnormalities relieved significantly. And compared with blood-activating drug group, the combined administration group demonstrated further increase in the values of EF and FS and further decrease in LVIDd and LVIDs (P < 0.05), the collagen deposition and myocardium fibrosis, the disorganized sarcomeres and mitochondrial abnormalities relieved even more in Huoxue plus Anshen prescription group.

CONCLUSIONThe combined administration of blood-activating drug and sedative drug can further improve cardiac structure and function after myocardial ischemia infarction and have an obvious synergistic effect which may be related to sedative drug's effect of resisting lipid peroxide, stabling myocardial cell membrane and mitochondrial membrane and relieving cardiac cell injury.

Animals ; Drugs, Chinese Herbal ; therapeutic use ; Hypnotics and Sedatives ; therapeutic use ; Lipid Peroxidation ; drug effects ; Male ; Myocardial Infarction ; drug therapy ; pathology ; Rats ; Rats, Wistar

OBJECTIVE: To determine the regulation effect of bone morphogenetic protein-4 (BMP-4) on the proliferation and differentiation of rat hepatic precursor cells. METHODS: We used Noggin (200 ng/mL) as the function blocking control of BMP-4, and the hepatic precursor cells of WB-F344 were treated with recombinant BMP-4 at 50 ng/mL at different time points. The proliferation of WB-F344 cells were tested by methyl thiazolyl tetrazolium (MTT) colorimetric assay. The ultrastructural characters of differentiated WB-F344 cells regulated by BMP-4 were observed under a transmission electron microscope. RT-PCR was used to examine mRNA expression of specific molecular markers for different cellular phenotypes potentially differentiated from the WB-F344 cells. RESULTS: At different time points, the absorbance values in the BMP-4 treatment groups were higher than those in the control groups of Noggin and blank treatment (P<0.01). The WB-F344 cells treated with BMP-4 exhibited typical ultrastructural characters of well-differentiated epithelial cells. The hepatocyte mRNA markers were more significantly promoted in the differentiated WB-F344 cells in the BMP-4 treatment group than those in the other 2 control groups. CONCLUSION BMP-4 can promote the proliferation and directional differentiation towards hepatocytes of rat hepatic precursor cells of WB-F344.
Animals ; Bone Morphogenetic Protein 4 ; antagonists & inhibitors ; genetics ; physiology ; Carrier Proteins ; pharmacology ; Cell Differentiation ; Cell Line ; Cell Proliferation ; Hepatocytes ; cytology ; Rats ; Recombinant Proteins ; Stem Cells ; cytology

OBJECTIVE: To determine the treatment effects of transplanted hepatic progenitor cells (WB-F344 cells) combined with heparin on the acute liver injury in SD rats. METHODS: A total of 2*10(7) hepatic stem cells (WB-F344) infected with GFP lentivirus and 8 μL heparin were transplanted through the spleen in SD rats with acute liver injury, which was induced by an intraperitoneal injection of CCl4. The liver and spleen tissues underwent fluorescence examination 1 day after the transplantation. The liver functions were tested, and the liver tissues were histopathologically examined on the 3rd, 7th, 14th, and 28th day of the cell transplantation. RESULTS: The transfected WB-F344 cells expressed GFP 3 days after the lentivirus infection and were found in the rat liver 1 day after the WB-F344 transplantation. The liver function and histopathological recovery of the liver tissues in the group of WB-F344 transplantation were better than those of the control group (P<0.05). CONCLUSION Transplantation of hepatic stem cells combined with heparin can promote the liver recovery in rats with acute liver injury induced by CCl4.
Animals ; Carbon Tetrachloride ; Carbon Tetrachloride Poisoning ; Heparin ; therapeutic use ; Hepatocytes ; transplantation ; Liver Failure, Acute ; chemically induced ; therapy ; Male ; Rats ; Rats, Sprague-Dawley ; Stem Cell Transplantation ; methods

0.10), but more significant after the causes of DIC were corrected( P