Objective:To explore the configurational factors influencing the professional commitment of Master of Nursing Specialist (MNS) students and identify effective paths to enhance their professional commitment.Methods:A convenience sample of MNS students from six hospitals in Jiangsu Province, Shanghai City, and Anhui Province was surveyed between December 2023 and October 2024. Data were collected using a general information questionnaire, the Nurses' Professional Commitment Scale (NPCS), the Connor-Davidson Resilience Scale (CD-RISC-10), the Multidimentional Perceived Social Support (MSPSS), and the Vocational Outcome Expectation Scale (VOE-R). Fuzzy-set Qualitative Comparative Analysis (fsQCA) was used to explore the configurational influences on professional commitment.Results:A total of 332 questionnaires were distributed, and 302 valid responses were obtained (effective response rate: 90.1%). The mean NPCS score among respondents was (89.30±22.42), indicating a upper-middle level of professional commitment. The fsQCA results revealed that the frequency of night shift rotation was a necessary condition affecting professional commitment. A total of three distinct configurations (paths) were identified that can enhance the professional commitment of MNS students.Conclusions:The professional commitment of MNS students is influenced by multiple interacting factors. It is recommended that hospitals, universities, and nursing educators develop targeted interventions based on these identified configurations to improve professional commitment.

Objective To study and compare the myopia control effects of orthokeratology(OK)lenses with a small treatment zone and peripheral defocus spectacle lenses in children aged 8-12 years.Methods A prospective study was made on 120 myopic children(120 myopic eyes were all right eyes)aged 8-12 years who visited the Optometry Clinic of the First Affiliated Hospital of Baotou Medical College between September 2022 and April 2023.The patients were randomly divided into a single vision lens(SVL)group,a highly aspherical lenslets(HAL)group,a 6.2 mm-BOZD OK group and a 5.0 mm-BOZD OK group,with 30 cases(30 eyes)in each group.After 12 months of follow-up,the spherical equivalent(SE),axial length(AL),cylinder diopter and their variations were compared among the four groups at different time points before and after treatment.Results There were no significant differences in SE and AL among the four groups be-fore and 3 months after treatment(all P＞0.05).After 6 months of treatment,there were no statistically significant differ-ences in SE among the four groups(P＞0.05),but significant differences were observed in AL among the four groups(P＜0.05).Pairwise comparisons revealed that the AL in the 5.0 mm-BOZD OK group was significantly lower than that in the SVL group(P＜0.05).There was no significant difference between each two of the other groups(all P＞0.05).After 12 months of treatment,significant differences in SE and AL were observed among the four groups(all P＜0.05).Pairwise comparisons revealed that compared with the SVL group,the HAL,6.2 mm-BOZD OK,and 5.0 mm-BOZD OK groups ex-hibited a statistically significant increase in SE and decrease in AL(all P＜0.05).However,there was no significant differ-ence in SE and AL among HAL,6.2 mm-BOZD OK,and 5.0 mm-BOZD OK groups(all P＞0.05).After 12 months of treat-ment,there were significant differences in the changes of SE and AL among the four groups(all P＜0.05).The changes of SE and AL in HAL,6.2 mm-BOZD OK,and 5.0 mm-BOZD OK groups were significantly less than those in the SVL group(all P＜0.05).The changes of SE and AL in the 5.0 mm-BOZD OK group were significantly less than those in HAL and 6.2 mm-BOZD OK groups(all P＜0.05).There was no significant difference in the changes of SE and AL between HAL and 6.2 mm-BOZD OK groups(both P＞0.05).There were no significant differences in cylinder diopter among the four groups be-fore and 12 months after treatment.In the SVL group,there was no significant difference in cylinder diopter before and 12 months after treatment.The cylinder diopters of HAL,6.2 mm-BOZD OK and 5.0 mm-BOZD OK groups were significantly decreased 12 months after treatment,compared with those before treatment.Conclusion HAL,6.2 mm-BOZD OK len-ses and 5.0 mm-BOZD OK lenses can effectively control the progression of myopia in children aged 8-12 years.Among them,5.0 mm-BOZD OK lenses exhibit the most significant control effect,while HAL and 6.2 mm-BOZD OK lenses have similar myopia control effects.

Objective:To investigate the role and underlying mechanism of protein arginine methyltransferase 1 (PRMT1) and its inhibitor in alkali burn-induced corneal neovascularization (CNV).Methods:Seventy-two SPF-grade C57BL/6 mice were randomly divided into a normal group and 1 day post-modeling, 4 days post-modeling, and 7 days post-modeling groups to establish an alkali burn-induced CNV model and determine the optimal time point for analysis.Another 90 mice were randomly assigned to five groups: alkali burn group, dimethyl sulfoxide (DMSO) group, PRMT1 inhibitor group, fibroblast growth factor 2 (FGF2) inhibitor group, and PRMT1 inhibitor combined with FGF2 group to evaluate the role of PRMT1 in CNV.Human umbilical vein endothelial cells (HUVECs) and murine macrophage-like RAW264.7 cells were used to establish a hypoxia/reoxygenation (H/R)-induced in vitro model to mimic the ischemic microenvironment.Cells were assigned to the following groups: control group, H/R group, H/R+ DMSO group, H/R+ si-NC group, H/R+ si-PRMT1 group, H/R+ si-FGF2 group, H/R+ PRMT1 inhibitor group, and H/R+ PRMT1 inhibitor+ FGF2 group.Corneal opacity and CNV areas were assessed by slit-lamp microscopy.Corneal structural changes and inflammatory cell count were determined by hematoxylin and eosin staining.PRMT1-positive cell count was determined by immunohistochemistry and the expression of PRMT1, CD31, vascular endothelial growth factor (VEGF), F4/80, CD206, and inducible nitric oxide synthase (iNOS) was assessed by immunofluorescence staining.The expression levels of macrophage markers, including F4/80, iNOS, CD206, interleukin-10 (IL-10), and arginase-1 (Arg-1), were quantified by real-time quantitative PCR and Western blot.Cell proliferation, migration, and angiogenic capacity were evaluated by functional assays including the CCK-8 assay, wound healing assay, Transwell migration assay, and tube formation assay.The research process followed the relevant regulations of the Visual and Ophthalmology Association, and the research plan was approved by the Laboratory Animal Committee of Wuhan University (No.20220504A). Results:Compared with the normal group, the 7 days post-modeling group showed significantly increased corneal opacity scores and CNV area, upregulated VEGF expression, and increased inflammatory cells (all P<0.05).The number of PRMT1-positive cells in the alkali burn group was (39.67±3.51) cells/visual field, which was significantly higher than (3.33±0.58) cells/visual field in the normal group ( t=17.68, P<0.01).Both mRNA and protein expression levels of PRMT1 and FGF2 were significantly elevated in the alkali burn group compared with the normal group (all P<0.01).Compared with the alkali burn group, the PRMT1 inhibitor group showed reduced corneal opacity scores, decreased CNV area, fewer inflammatory cells, and lower expression levels of PRMT1, FGF2, VEGF, Arg-1, IL-10 proteins, as well as CD206 mRNA (all P<0.05).Cell viability, migration distance, migration number, and tubes formed were significantly increased in the H/R group compared with the control group, significantly reduced in the H/R+ si-PRMT1 and H/R+ PRMT1 inhibitor groups compared with the H/R group and significantly increased in H/R+ PRMT1 inhibitor+ FGF2 group than in H/R+ PRMT1 inhibitor group (all P<0.05).Compared with the H/R group, the H/R+ PRMT1 inhibitor group exhibited reduced expression of FGF2, VEGFA, p-PI3K, and p-Akt, while those were upregulated in the H/R+ PRMT1 inhibitor+ FGF2 group compared with the H/R+ PRMT1 inhibitor group (all P<0.05).The proportions of CD206-positive cells in the H/R, H/R+ DMSO, H/R+ PRMT1 inhibitor, and H/R+ PRMT1 inhibitor+ FGF2 groups were all significantly higher than those in the control group, and significantly higher in the H/R, H/R+ DMSO, and H/R+ PRMT1 inhibitor+ FGF2 groups compared with the H/R+ PRMT1 inhibitor group (all P<0.05).Compared with the alkali burn group, the FGF2 inhibitor group, PRMT1 inhibitor group, and PRMT1 inhibitor+ FGF2 group all showed reduced corneal opacity scores, CNV area, and decreased number of VEGFA-, CD206-, and F4/80-positive cells, with the above indicators being lower in the PRMT1 inhibitor group compared with the FGF2 inhibitor and PRMT1 inhibitor+ FGF2 groups and higher in PRMT1 inhibitor+ FGF2 group than in the FGF2 inhibitor group (all P<0.05).Compared with the alkali burn group, the PRMT1 inhibitor group had decreased protein expression levels of FGF2, p-PI3K, p-Akt, CD31, VEGFA and Arg-1, with higher protein expression levels in the PRMT1 inhibitor+ FGF2 group than in the PRMT1 inhibitor group (all P<0.05). Conclusions:PRMT1 may regulate macrophage activation and anti-inflammatory polarization via the FGF2/PI3K/Akt signaling pathway, thereby promoting the occurrence and development of CNV.Targeted inhibition of PRMT1 may serve as an effective therapeutic strategy for CNV.

Objective:To investigate the basic information of diagnostic and therapeutic nuclear medicine resources and practices in Henan province in 2022, and to assess the equity of their distribution, and to compare them with the 1998 survey result, so as to provide the reference for the allocation of nuclear medicine resources in Henan province.Methods:On-site investigations were conducted on medical institutions in Henan province that carry out diagnostic and therapeutic nuclear medicine practices. By using a combination of Lorenz curve, Gini coefficient and Theil index, the fairness of nuclear medicine resources and practices were assessed based on population distribution in Henan province in 1998 and 2022.Results:In 2022, there were 54 medical institutions in Henan province that carried out diagnostic and therapeutic nuclear medicine practices, with 67 items of nuclear medicine imaging and supporting equipment, and 690 nuclear medicine staff members involved. A total of 200 938 diagnostic clinical nuclear medicine imaging practices were performed throughout the year, with a diagnostic frequency of 2.04 per 1 000 population. Various types of therapeutic nuclear medicine practices were performed on 29 044 individuals, with a frequency of 0.29 per 1 000 population. The Gini coefficients for the distribution of diagnostic nuclear medicine practices, nuclear medicine staff, and practice frequency based on population distribution in Henan province in 1998 were 0.489, 0.505, and 0.526, respectively, and the Theil indices were 0.091 8, 0.172 1, and 0.139 7, respectively. The Gini coefficients for the distribution of nuclear diagnostic medicine equipment, nuclear medicine staff, and practice frequency based on population distribution in Henan province in 2022 were 0.244, 0.246, and 0.363, respectively, and the Theil indices were 0.086 8, 0.145 3, and 0.159 9, respectively.Conclusions:While clinical nuclear medicine in Henan province is rapidly developing, the distribution of various types of nuclear medicine resources and practices is becoming increasingly balanced. However, there still exists a certain degree of imbalance. Additionally, this also demonstrates a correlation between the resource distribution and regional economic development. The rationality of resource allocation needs further optimization.

Thoracic anesthesia with spontaneous respiration represents a form of precision anesthesia meticulously customized to individual patients.Considering the more stringent requirements this anesthesia approach imposes on the regulation of respiratory function,the writing group of the"Consensus of Experts on the Management of Thoracic Anesthesia with Spontaneous Respiration"has formulated elaborate guidelines regarding indications and contraindications,preoperative evaluation,anesthesia implementation,common complications,and treatment strategies.This was accomplished by referencing relevant domestic and international literature and integrating it with actual clinical requirements.The objective is to standardize the rational application of this anesthesia method.

Objective:To explore the configurational factors influencing the professional commitment of Master of Nursing Specialist (MNS) students and identify effective paths to enhance their professional commitment.Methods:A convenience sample of MNS students from six hospitals in Jiangsu Province, Shanghai City, and Anhui Province was surveyed between December 2023 and October 2024. Data were collected using a general information questionnaire, the Nurses' Professional Commitment Scale (NPCS), the Connor-Davidson Resilience Scale (CD-RISC-10), the Multidimentional Perceived Social Support (MSPSS), and the Vocational Outcome Expectation Scale (VOE-R). Fuzzy-set Qualitative Comparative Analysis (fsQCA) was used to explore the configurational influences on professional commitment.Results:A total of 332 questionnaires were distributed, and 302 valid responses were obtained (effective response rate: 90.1%). The mean NPCS score among respondents was (89.30±22.42), indicating a upper-middle level of professional commitment. The fsQCA results revealed that the frequency of night shift rotation was a necessary condition affecting professional commitment. A total of three distinct configurations (paths) were identified that can enhance the professional commitment of MNS students.Conclusions:The professional commitment of MNS students is influenced by multiple interacting factors. It is recommended that hospitals, universities, and nursing educators develop targeted interventions based on these identified configurations to improve professional commitment.

Objective:To investigate the role and underlying mechanism of protein arginine methyltransferase 1 (PRMT1) and its inhibitor in alkali burn-induced corneal neovascularization (CNV).Methods:Seventy-two SPF-grade C57BL/6 mice were randomly divided into a normal group and 1 day post-modeling, 4 days post-modeling, and 7 days post-modeling groups to establish an alkali burn-induced CNV model and determine the optimal time point for analysis.Another 90 mice were randomly assigned to five groups: alkali burn group, dimethyl sulfoxide (DMSO) group, PRMT1 inhibitor group, fibroblast growth factor 2 (FGF2) inhibitor group, and PRMT1 inhibitor combined with FGF2 group to evaluate the role of PRMT1 in CNV.Human umbilical vein endothelial cells (HUVECs) and murine macrophage-like RAW264.7 cells were used to establish a hypoxia/reoxygenation (H/R)-induced in vitro model to mimic the ischemic microenvironment.Cells were assigned to the following groups: control group, H/R group, H/R+ DMSO group, H/R+ si-NC group, H/R+ si-PRMT1 group, H/R+ si-FGF2 group, H/R+ PRMT1 inhibitor group, and H/R+ PRMT1 inhibitor+ FGF2 group.Corneal opacity and CNV areas were assessed by slit-lamp microscopy.Corneal structural changes and inflammatory cell count were determined by hematoxylin and eosin staining.PRMT1-positive cell count was determined by immunohistochemistry and the expression of PRMT1, CD31, vascular endothelial growth factor (VEGF), F4/80, CD206, and inducible nitric oxide synthase (iNOS) was assessed by immunofluorescence staining.The expression levels of macrophage markers, including F4/80, iNOS, CD206, interleukin-10 (IL-10), and arginase-1 (Arg-1), were quantified by real-time quantitative PCR and Western blot.Cell proliferation, migration, and angiogenic capacity were evaluated by functional assays including the CCK-8 assay, wound healing assay, Transwell migration assay, and tube formation assay.The research process followed the relevant regulations of the Visual and Ophthalmology Association, and the research plan was approved by the Laboratory Animal Committee of Wuhan University (No.20220504A). Results:Compared with the normal group, the 7 days post-modeling group showed significantly increased corneal opacity scores and CNV area, upregulated VEGF expression, and increased inflammatory cells (all P<0.05).The number of PRMT1-positive cells in the alkali burn group was (39.67±3.51) cells/visual field, which was significantly higher than (3.33±0.58) cells/visual field in the normal group ( t=17.68, P<0.01).Both mRNA and protein expression levels of PRMT1 and FGF2 were significantly elevated in the alkali burn group compared with the normal group (all P<0.01).Compared with the alkali burn group, the PRMT1 inhibitor group showed reduced corneal opacity scores, decreased CNV area, fewer inflammatory cells, and lower expression levels of PRMT1, FGF2, VEGF, Arg-1, IL-10 proteins, as well as CD206 mRNA (all P<0.05).Cell viability, migration distance, migration number, and tubes formed were significantly increased in the H/R group compared with the control group, significantly reduced in the H/R+ si-PRMT1 and H/R+ PRMT1 inhibitor groups compared with the H/R group and significantly increased in H/R+ PRMT1 inhibitor+ FGF2 group than in H/R+ PRMT1 inhibitor group (all P<0.05).Compared with the H/R group, the H/R+ PRMT1 inhibitor group exhibited reduced expression of FGF2, VEGFA, p-PI3K, and p-Akt, while those were upregulated in the H/R+ PRMT1 inhibitor+ FGF2 group compared with the H/R+ PRMT1 inhibitor group (all P<0.05).The proportions of CD206-positive cells in the H/R, H/R+ DMSO, H/R+ PRMT1 inhibitor, and H/R+ PRMT1 inhibitor+ FGF2 groups were all significantly higher than those in the control group, and significantly higher in the H/R, H/R+ DMSO, and H/R+ PRMT1 inhibitor+ FGF2 groups compared with the H/R+ PRMT1 inhibitor group (all P<0.05).Compared with the alkali burn group, the FGF2 inhibitor group, PRMT1 inhibitor group, and PRMT1 inhibitor+ FGF2 group all showed reduced corneal opacity scores, CNV area, and decreased number of VEGFA-, CD206-, and F4/80-positive cells, with the above indicators being lower in the PRMT1 inhibitor group compared with the FGF2 inhibitor and PRMT1 inhibitor+ FGF2 groups and higher in PRMT1 inhibitor+ FGF2 group than in the FGF2 inhibitor group (all P<0.05).Compared with the alkali burn group, the PRMT1 inhibitor group had decreased protein expression levels of FGF2, p-PI3K, p-Akt, CD31, VEGFA and Arg-1, with higher protein expression levels in the PRMT1 inhibitor+ FGF2 group than in the PRMT1 inhibitor group (all P<0.05). Conclusions:PRMT1 may regulate macrophage activation and anti-inflammatory polarization via the FGF2/PI3K/Akt signaling pathway, thereby promoting the occurrence and development of CNV.Targeted inhibition of PRMT1 may serve as an effective therapeutic strategy for CNV.

Objective:To investigate the basic information of diagnostic and therapeutic nuclear medicine resources and practices in Henan province in 2022, and to assess the equity of their distribution, and to compare them with the 1998 survey result, so as to provide the reference for the allocation of nuclear medicine resources in Henan province.Methods:On-site investigations were conducted on medical institutions in Henan province that carry out diagnostic and therapeutic nuclear medicine practices. By using a combination of Lorenz curve, Gini coefficient and Theil index, the fairness of nuclear medicine resources and practices were assessed based on population distribution in Henan province in 1998 and 2022.Results:In 2022, there were 54 medical institutions in Henan province that carried out diagnostic and therapeutic nuclear medicine practices, with 67 items of nuclear medicine imaging and supporting equipment, and 690 nuclear medicine staff members involved. A total of 200 938 diagnostic clinical nuclear medicine imaging practices were performed throughout the year, with a diagnostic frequency of 2.04 per 1 000 population. Various types of therapeutic nuclear medicine practices were performed on 29 044 individuals, with a frequency of 0.29 per 1 000 population. The Gini coefficients for the distribution of diagnostic nuclear medicine practices, nuclear medicine staff, and practice frequency based on population distribution in Henan province in 1998 were 0.489, 0.505, and 0.526, respectively, and the Theil indices were 0.091 8, 0.172 1, and 0.139 7, respectively. The Gini coefficients for the distribution of nuclear diagnostic medicine equipment, nuclear medicine staff, and practice frequency based on population distribution in Henan province in 2022 were 0.244, 0.246, and 0.363, respectively, and the Theil indices were 0.086 8, 0.145 3, and 0.159 9, respectively.Conclusions:While clinical nuclear medicine in Henan province is rapidly developing, the distribution of various types of nuclear medicine resources and practices is becoming increasingly balanced. However, there still exists a certain degree of imbalance. Additionally, this also demonstrates a correlation between the resource distribution and regional economic development. The rationality of resource allocation needs further optimization.

Objective To study and compare the myopia control effects of orthokeratology(OK)lenses with a small treatment zone and peripheral defocus spectacle lenses in children aged 8-12 years.Methods A prospective study was made on 120 myopic children(120 myopic eyes were all right eyes)aged 8-12 years who visited the Optometry Clinic of the First Affiliated Hospital of Baotou Medical College between September 2022 and April 2023.The patients were randomly divided into a single vision lens(SVL)group,a highly aspherical lenslets(HAL)group,a 6.2 mm-BOZD OK group and a 5.0 mm-BOZD OK group,with 30 cases(30 eyes)in each group.After 12 months of follow-up,the spherical equivalent(SE),axial length(AL),cylinder diopter and their variations were compared among the four groups at different time points before and after treatment.Results There were no significant differences in SE and AL among the four groups be-fore and 3 months after treatment(all P＞0.05).After 6 months of treatment,there were no statistically significant differ-ences in SE among the four groups(P＞0.05),but significant differences were observed in AL among the four groups(P＜0.05).Pairwise comparisons revealed that the AL in the 5.0 mm-BOZD OK group was significantly lower than that in the SVL group(P＜0.05).There was no significant difference between each two of the other groups(all P＞0.05).After 12 months of treatment,significant differences in SE and AL were observed among the four groups(all P＜0.05).Pairwise comparisons revealed that compared with the SVL group,the HAL,6.2 mm-BOZD OK,and 5.0 mm-BOZD OK groups ex-hibited a statistically significant increase in SE and decrease in AL(all P＜0.05).However,there was no significant differ-ence in SE and AL among HAL,6.2 mm-BOZD OK,and 5.0 mm-BOZD OK groups(all P＞0.05).After 12 months of treat-ment,there were significant differences in the changes of SE and AL among the four groups(all P＜0.05).The changes of SE and AL in HAL,6.2 mm-BOZD OK,and 5.0 mm-BOZD OK groups were significantly less than those in the SVL group(all P＜0.05).The changes of SE and AL in the 5.0 mm-BOZD OK group were significantly less than those in HAL and 6.2 mm-BOZD OK groups(all P＜0.05).There was no significant difference in the changes of SE and AL between HAL and 6.2 mm-BOZD OK groups(both P＞0.05).There were no significant differences in cylinder diopter among the four groups be-fore and 12 months after treatment.In the SVL group,there was no significant difference in cylinder diopter before and 12 months after treatment.The cylinder diopters of HAL,6.2 mm-BOZD OK and 5.0 mm-BOZD OK groups were significantly decreased 12 months after treatment,compared with those before treatment.Conclusion HAL,6.2 mm-BOZD OK len-ses and 5.0 mm-BOZD OK lenses can effectively control the progression of myopia in children aged 8-12 years.Among them,5.0 mm-BOZD OK lenses exhibit the most significant control effect,while HAL and 6.2 mm-BOZD OK lenses have similar myopia control effects.

Thoracic anesthesia with spontaneous respiration represents a form of precision anesthesia meticulously customized to individual patients.Considering the more stringent requirements this anesthesia approach imposes on the regulation of respiratory function,the writing group of the"Consensus of Experts on the Management of Thoracic Anesthesia with Spontaneous Respiration"has formulated elaborate guidelines regarding indications and contraindications,preoperative evaluation,anesthesia implementation,common complications,and treatment strategies.This was accomplished by referencing relevant domestic and international literature and integrating it with actual clinical requirements.The objective is to standardize the rational application of this anesthesia method.