Objective: To analyze the epidemiological characteristics of hand, foot and mouth disease (HFMD) among children aged 0-9 years in Guangzhou, so as to provide reference basis for further exploring scientifically effective prevention and control measures. Methods: Reported cases of HFMD in children aged 0-9 years in Guangzhou City from 2013-2025 were selected from China disease prevention and control information system, and the incidence in different years was analyzed descriptively; the trends of the composition ratio and incidence in various age groups were analyzed by using the Joinpoint regression model, spatial autocorrelation analyses were performed at the district level, and the seasonal autoregressive integrated moving average model (SARIMA) was applied to predict annual incidence rate for 2026. Results: From 2013 to 2025, the average annual reported incidence rate of HFMD among children aged 0-9 years in Guangzhou was 2 513.98/100 000, showing a fluctuating trend. Peaks of incidence occurred from May to July and from September to October. The male to female ratio was 1.49∶1. The average annual incidence rate was 4 743.99/100 000 in the 0-1 years age group, 3 298.19/100 000 in the 2-5 years age group, and 544.22/ 100 000 in the 6-9 years age group. Joinpoint regression analysis showed a decreasing trend in the proportion of the 0-1 years age group (APC= -6.02 %, t =-3.77) and an increasing trend in the proportion of the 6-9 years age group (APC=16.39%, t =5.67) (both P < 0.05 ). No significant global spatial clustering was observed at the district level; however, local scattered clusters were identified in certain areas. Using the SARIMA(2,0,0)(2,0,0) 12 model, the predicted annual incidence rate of HFMD among children aged 0-9 years in Guangzhou in 2026 was 2 680.56/100 000. Conclusions From 2013 to 2025, the annual incidence rate of HFMD among children aged 0-9 years in Guangzhou exhibited a fluctuating trend; the highest incidence rate is observed in the 0-1 years age group, while the proportion of cases in the 6-9 years age group increase year by year;and local spatial clusters of abnormal distribution are identified. It is essential to enhance risk monitoring and health education in key populations and areas to mitigate the risk of infection in children.

Cataract is the world's leading cause of blindness, and surgery is the most effective treatment for cataract. With the development of femtosecond laser technology and ophthalmic surgical equipment, the application of femtosecond laser systems in cataract surgery is becoming increasingly widespread. It can be used in cataract surgery for corneal incisions, anterior capsulotomy, lens fragmentation, arcuate incisions and other key operations. Compared to traditional surgery, femtosecond laser assisted cataract surgery(FLACS)offers significant advantages in precision, safety and postoperative visual outcomes. Its clinical benefits have garnered growing recognition among ophthalmologists. However, the key technologies and high-precision equipment for FLACS remain predominantly controlled by Western countries. In China, the research in this field began later. This article reviews the technological advancements in FLACS, with a focus on femtosecond laser technology, optical coherence tomography(OCT), artificial intelligence, and clinical application progress. The objective is to provide theoretical foundations and practical insights for the development of ophthalmic medical technology in China.

Objective To establish human colorectal cancer(CRC)organoids and to evaluate the efficacy of chem-otherapy drugs.Methods Patient-derived CRC cells were cultured to form organoids.The CRC organoids and origi-nal tissues were stained with molecular markers of CRC immunohishtochemically.CRC organoids were used to test drug sensitivity and different concentrations of chemotherapy drugs 5-fluorouracil,oxaliplatin and irinotecan were given respectively;Organoid activity before and after drug treatment was measured by 3D cell viability assay.Results The patient-derived organoids(PDO)from 5 CRC tissues were successfully established.The expression of CK20,Ki67 and Villin proteins was similar in organoids and in original tumor.The organoids retained histologcial features similar to those of the original tumors.Different PDO showed differential sensitivity to different chemothera-py drugs.Conclusions CRC-PDO can dispaly their different sensitivities to different chemotherapy drugs,and could provide valuble reference for personalized treatment for CRC patients.

OBJECTIVE: To observe the effect of the direct stimulation of acupuncture at extraocular muscle attachment point on acquired extraocular muscle palsy. METHODS: Thirteen patients with acquired extraocular muscle palsy were treated with acupuncture directly at extraocular muscle (paralytic muscle) attachment point. Firstly, the intraocular conjunctival sac drops of topical anesthetic (procaine hydrochloride eye drops) were administered, 0.2 mL each time, once every 10 minutes, for a total of 3 times. Acupuncture was delivered immediately after the third drop. The sterile acupuncture needle for single use, 0.25 mm×25 mm, was inserted at the anatomical location of the corneal limbal attachment of paralytic extraocular muscle, with an angle of 10° to 15° formed between the needle tip and extraocular muscle, and a depth of 0.3 mm to 0.5 mm. Pivoted by the needle tip, the eyeball was moved passively towards the direction of normal action of orbital muscle, 30 to 50 times until the patient felt soreness of the eyeball; afterwards, the needle was removed. After acupuncture, levofloxacin eye drops were administered once (0.2 mL) at the affected eye. The treatment was given twice a week, and completed when diplopia disappeared. Before and after treatment, the diplopia and the synoptophore circumference were observed respectively. RESULTS: After 7 to 24 (15.46±5.56) times of direct stimulation with acupuncture at extraocular muscle attachment point, the symptoms of diplopia disappeared in 13 patients, the eye position restored to orthophoria, and the circumference of synoptophore was reduced to be (4.04±0.82)° from (19.38±3.98)° detected before treatment (P<0.05). CONCLUSION Acupuncture directly at extraocular muscle attachment can attenuate diplopia and improve ocular muscle function in patients with acquired extraocular muscle palsy.
Humans ; Acupuncture Therapy ; Male ; Female ; Middle Aged ; Adult ; Oculomotor Muscles/physiopathology* ; Aged ; Acupuncture Points ; Ophthalmoplegia/physiopathology*

OBJECTIVE To explore the therapeutic effect of hearing aids on chronic subjective tinnitus with moderate to severe sensorineural hearing loss.METHODS Ninety-five patients were enrolled in this prospective study.Pure tone audiometry,acoustic immittance and tinnitus were performed before hearing aids were worn.The tinnitus handicap inventory(THI),hospital anxiety and depression scale(HADS)and visual analogue scale(VAS)were completed before and after 3 and 6 months after wearing the device.SPSS 22 was used for statistical analysis.RESULTS After 3 months and 6 months of hearing aid wearing,THI,HADS and VAS scores of the patients were significantly decreased,and there were significant differences before and after wearing the hearing aid(P<0.05).The THI score of patients in the catastrophic group decreased the most,reaching 44.5 points.Age,gender,duration of tinnitus,the side of tinnitus and the degree of hearing loss have no effect on the treatment effect of hearing aids.CONCLUSION Hearing aids can not only improve the hearing of patients,but also reduce tinnitus,relieve anxiety and depression.

The Ehlers-Danlos syndrome(EDS)is a rare inherent connective tissue disorder.The prev-alence of EDS in the population is estimated at one out of ten thousand to one out of a hundred thousand.The vascular EDS(vEDS)are rare among the subtypes but are the worst in prognosis.The article reports a case of vEDS admitted to the hospital.The patient was a young man complaining of a sudden onset of aphasia in right hemiparalysis and severe left abdominal pain for unknown reasons.The diagnosis was made after the genetic testing.The patient suffered from vEDS.Then,the multi-disciplinary team(MDT)made a treatment plan tailored to this young patient.The complexity in classification and delusive presentations of the EDS make the correct diagnosis very challenging.This article hopes to report this case and to share the experiences to the bet-ter understanding of this disease.

Objective To establish a gas chromatography for simultaneous determination of camphor residue and borneolum content in Qingchang Suppository. Methods Gas chromatograph method was used. The chromatographic column was Agilent capillary column(30 m×0.25 mm×0.25 µm). The column temperature was 140 ℃. The sample injection temperature was 250 ℃. The FID detector temperature was 250 ℃. Results Camphor，borneol and isoborneol content showed good linear in the extent of 0.0299~1.497(r=1.000), 0.0205~1.025(r=1.000), 0.0097~0.4830 µg (r=1.000). RSDs of precision，stability and repeatability test results were less than 2%. The recovery was 99.7%, 101.0%, 102.5%. Conclusion This method is simple and quick with accurate result, which could be used for the content determination of Borneol in Qingchang Suppository.

Hospitals need to explore based on their own specifics how to establish a modern hospital governance system and how a lean hospital administration level be achieved. This paper introduced the practice and exploration of modern hospital governance system construction in Beijing Tsinghua Changgung Hospital as follows. The hospital is run by a board of administration featuring co-administration by both Beijing Municipal Hospital Administration Center and Tsinghua University; A hospital charter is developed and agreed upon to regulate decision-making, day-to-day operations and supervision; The hospital president′s responsibility is under the leadership of the hospital′s Communist Party Committee; Benefiting from best practices of Taiwan Changgung′s medical system, the hospital′s specialized medical teams and professional administrative teams operate in cooperative governance, and under professional management model and a lean operation management system. The practice and exploration of Beijing Tsinghua Changgung Hospital can serve as references for building and perfecting a modern hospital governance system.

Objective:To investigate the clinical effect of posterior tibial artery perforator propeller flap in repairing soft tissue injury of medial malleolus.Methods:From June 2016 to March 2019, five male patients with ankle trauma were treated in Ningbo Ninth Hospital, aged 30-64 years (average 45 years). There were 3 cases of right and 2 cases of left. Causes: traffic injury in 2 cases, crush injury in 3 cases. There were 4 cases with fracture, 1 case with plate and bone exposure, 2 cases with local infection and 1 case with skin avulsion. Preoperative soft tissue defect area: 2.0 cm × 3.0 cm-4.0 cm × 6.0 cm. After debridement, the posterior tibial artery perforator propeller flap was designed to repair the wound. During the operation, the target perforator was searched between the deep fascia superficial flexor longus and digitorum flexor longus, and the perforator pedicle was fully exposed carrying a small amount of fascial tissue and the length of the pedicle ≥ 2 cm. After the skin flap was completely cut from the superficial layer of the deep fascia, the skin flap was rotated to cover the wound. After confirmation of good blood supply of the skin flap, the wound was loosely sutured, and the donor site was directly sutured or covered with skin grafts. Routine fluid rehydration, anti-infection, anti-spasm, and anti-thrombosis treatments were performed postoperatively. The ankle joint was raised in a neutral position and immobilized with plaster. The temperature, color, tension and reflux of the skin flap were monitored, and rehabilitation exercises were gradually carried out. The patient’s postoperative repair effect was observed.Results:All the flaps survived. The distance between the perforator and the medial malleolus was about 5.5-8.5 cm. The area of the flap was 4.0 cm × 6.5 cm-4.0 cm × 10.0 cm. The donor sites were sutured directly except one case. No flap related complications occurred during the follow-up period of 3-16 months (average of 12 months). No obvious scar hyperplasia was observed in flaps, which was similar to the adjacent skin characteristics, ankle joint movement could be achieved, and the skin flap could restore the protective feeling.Conclusions:The propeller flap with perforating branch of posterior tibial artery characterizes simple operation, similar soft tissue characteristics, stable and reliable blood supply, less damage to the donor and recipient sites, and less complications. It is one of the ideal methods to repair the soft tissue defects of the medial malleolus.

Objective:To investigate the clinical effect of posterior tibial artery perforator propeller flap in repairing soft tissue injury of medial malleolus.Methods:From June 2016 to March 2019, five male patients with ankle trauma were treated in Ningbo Ninth Hospital, aged 30-64 years (average 45 years). There were 3 cases of right and 2 cases of left. Causes: traffic injury in 2 cases, crush injury in 3 cases. There were 4 cases with fracture, 1 case with plate and bone exposure, 2 cases with local infection and 1 case with skin avulsion. Preoperative soft tissue defect area: 2.0 cm × 3.0 cm-4.0 cm × 6.0 cm. After debridement, the posterior tibial artery perforator propeller flap was designed to repair the wound. During the operation, the target perforator was searched between the deep fascia superficial flexor longus and digitorum flexor longus, and the perforator pedicle was fully exposed carrying a small amount of fascial tissue and the length of the pedicle ≥ 2 cm. After the skin flap was completely cut from the superficial layer of the deep fascia, the skin flap was rotated to cover the wound. After confirmation of good blood supply of the skin flap, the wound was loosely sutured, and the donor site was directly sutured or covered with skin grafts. Routine fluid rehydration, anti-infection, anti-spasm, and anti-thrombosis treatments were performed postoperatively. The ankle joint was raised in a neutral position and immobilized with plaster. The temperature, color, tension and reflux of the skin flap were monitored, and rehabilitation exercises were gradually carried out. The patient’s postoperative repair effect was observed.Results:All the flaps survived. The distance between the perforator and the medial malleolus was about 5.5-8.5 cm. The area of the flap was 4.0 cm × 6.5 cm-4.0 cm × 10.0 cm. The donor sites were sutured directly except one case. No flap related complications occurred during the follow-up period of 3-16 months (average of 12 months). No obvious scar hyperplasia was observed in flaps, which was similar to the adjacent skin characteristics, ankle joint movement could be achieved, and the skin flap could restore the protective feeling.Conclusions:The propeller flap with perforating branch of posterior tibial artery characterizes simple operation, similar soft tissue characteristics, stable and reliable blood supply, less damage to the donor and recipient sites, and less complications. It is one of the ideal methods to repair the soft tissue defects of the medial malleolus.