Chronic heart failure （CHF） is a major global public health problem， and myocardial infarction is one of its main causes. The mouse model of heart failure induced by coronary artery ligation is widely used in the study of CHF， while the TCM syndrome attributes of this model have not yet been clarified. According to the theory of correspondence between prescriptions and syndromes， the method of syndrome identification by prescription efficacy is an important means of current syndrome research of animal models. This method deduces the syndrome characteristics of animal models through prescription efficacy. Taking the four basic syndrome elements of Qi， blood， Yin and Yang as the classification reference， this study used coronary artery ligation to construct a mouse model of CHF and treated the model with four representative TCM injections with the effects of replenishing Qi， warming Yang， nourishing Yin， and activating blood and enalapril. Echocardiography， tongue color parameters， histopathology， serum N-terminal pro-brain natriuretic peptide （NT-proBNP） and cardiac troponin Ⅰ （cTnⅠ） levels， and systematically explored the TCM syndrome attributes of this model. The results showed that the coronary ligation model presented an obvious cardiac function decline， myocardial fibrosis， infarct size expansion， and purple dark tongue， which were consistent with the basic syndrome characteristics of blood stasis in CHF. Danhong injection had significant effects of improving the cardiac function， alleviating myocardial fibrosis， and reducing serum NT-proBNP and cTnⅠ levels. Huangqi Injection and Shenfu injection can improve the cardiac function and tongue color parameters， with limited effects. The effect of Shenmai injection group was not obvious. This study verifies that the established model conforms to blood stasis syndrome through the method of syndrome identification by prescription efficacy， which provides an experimental basis for the study of TCM syndrome mechanism of CHF.

Objective To analyze the research status and hotspots of Poria by means of bibliometrics and visualization methods;To provide reference for scientific research and clinical practice.Methods The research literature about Poria included in CNKI,Wanfang Data,VIP,CBM,Web of Science core collection and PubMed database was retrieved from 2004 to 2023.NoteExpress3.6.0.9155 software was used to de-duplicate,and Excel2016,CiteSpace 6.2.R4 and VOSviewer 1.6.18.0 software were used to analyze the annual number of publications,institutions,journals,authors and keywords,and visual display was demonstrated.Results Totally 3 861 Chinese papers and 477 English papers were included after screening,and the annual number of papers gradually increased.The institutions that published the most Chinese and English papers were Jiangsu Kangyuan Pharmaceutical Co.,LTD.and Chinese Academy of Sciences;the authors that publish the most Chinese and English papers were Wang Zhenzhong and Chen Lin;the journals that published the most Chinese and English papers were Zhong Cao Yao and Journal of Ethnopharmacology.Keyword analysis showed higher frequency of uterine fibroids,clinical efficacy,polysaccharide and antioxidant activity.20 clusters and 36 emergent keywords were generated.Conclusion The research focus in this field is on the pharmacological components and mechanism,clinical application and data mining of Poria.

Objective:To investigate the efficacy of lightroom therapy on depressive mood and sleep problems in patients with depression, and the potential effects on physiological indices related to circadian rhythms.Methods:From October 2021 to July 2023, 54 patients with acute-phase depression hospitalized in the Mental Health Center of the First Hospital of Hebei Medical University were recruited. The participants were randomly assigned to either medication combined with the bright light therapy group (bright light group, n=36) or medication combined with the dim light therapy group (dim light group, n=18). Both groups received light therapy for 2 weeks, at 10 000 lx in the bright light group and 300 lx in the dim light group. Both groups received 30 minutes of light therapy from 7:30-8:00 a.m daily over two weeks, followed up for 1 week post-treatment. The Hamilton Depression Rating Scale (HAMD 17) was used to assess patients′ depressive symptoms, and the Pittsburgh Sleep Quality Index (PSQI) was used to assess patients′ sleep quality at baseline, at the end of every week. The 32-Item Hypomania Checklist (HCL-32) was used at the end of week 2 to assess the risk of manic switching after treatment. Daily measurements of body temperature, heart rate, and blood pressure were taken before and after light therapy, along with recording adverse events related to the therapy. Paired t- tests were used to compare changes in physiological indicators before and after treatment, and repeated measures ANOVA was applied to compare clinical symptom changes between the two groups. Results:Thirty-one and fifteen patients completed this study in the bright light and dim light groups, respectively, with no statistically significant difference in dropout rates( P>0.05). There were significant interaction effects between the time and group for HAMD 17 and PSQI score( F=5.51,4.11, both P<0.05). Both groups showed significant reductions in HAMD 17 and PSQI scores at baseline, week 1, week 2, and week 3 ( P<0.001). In the bright light group, body temperature increased significantly post-treatment on days 1-4, day 7, and day 12 (all P<0.05). Heart rate elevated on day 5 ( P<0.05).Systolic blood pressure decreased on days 4, 5, 11, and 12 compared to the pre-treatment baseline(all P<0.05). In the dim light group, systolic blood pressure increased on day 11 ( P<0.05). Diastolic blood pressure in the bright light group decreased on days 1, 5, and 6( P<0.05). No serious adverse events, vision loss, ocular structural changes occurred in either group. No hypomania or mania episodes were observed. The incidence of adverse events did not differ significantly ( P>0.05). Conclusion:Medication combined with indoor bright light is more effective than the combination of dim light for depressive symptoms and sleep problems in patients with depression. Patients receiving bright light also may exhibit a higher body temperature, accelerated heart rate, and reduced blood pressure.

Objective To evaluate the clinical efficacy of Jiannao Kaiqiao Acupuncture combined with sensory integration training(SIT)in children with autism spectrum disorder(ASD)based on the brain-gut interaction theory.Methods A total of 135 children diagnosed with ASD at the Shenzhen Longgang Maternal and Child Health Hospital from March 2021 to March 2024 were enrolled.The patients were randomly divided into an observation group and a control group,with 68 patients in each group.The control group was given SIT treatment,while the observation group was treated with Jiannao Kaiqiao Acupuncture plus SIT,both groups were treated for 3 months.The changes in Childhood Autism Rating Scale(CARS)scores,Autism Behavior Checklist(ABC)scores,developmental quotient(DQ),serum neurofilament light chain(NFL),S100 calcium-binding protein β(S100β),and gut microbiota(Lactobacillus,Enterococcus,Bifidobacterium,Escherichia coli)were observed.Results(1)Both groups showed significant improvements in CARS,ABC,and DQ scores(P＜0.05),the observation group showed superior improvements compared to the control group,the difference being statistically significant(P＜0.05).(2)Serum NFL and S100β levels improved in both groups(P＜0.05),the observation group showed superior improvements compared to the control group,the difference being statistically significant(P＜0.05).(3)Gut microbiota composition(Lactobacillus,Enterococcus,Bifidobacterium,Escherichia coli)significantly normalized in both groups(P＜0.05),the observation group showed superior improvements compared to the control group,the difference being statistically significant(P＜0.05).(4)The total efficacy rate was 94.06％(66/68)in the observation group versus 85.07％(57/67)in the control group,the observation group showed superior improvements compared to the control group,the difference being statistically significant(P＜0.05).Conclusion Jiannao Kaiqiao Acupuncture combined with SIT effectively enhances cognitive function,alleviates clinical symptoms,modulates serum NFL/S100β levels,and restores gut microbiota balance in children with ASD.

Objective To analyze the research status and hotspots of Poria by means of bibliometrics and visualization methods;To provide reference for scientific research and clinical practice.Methods The research literature about Poria included in CNKI,Wanfang Data,VIP,CBM,Web of Science core collection and PubMed database was retrieved from 2004 to 2023.NoteExpress3.6.0.9155 software was used to de-duplicate,and Excel2016,CiteSpace 6.2.R4 and VOSviewer 1.6.18.0 software were used to analyze the annual number of publications,institutions,journals,authors and keywords,and visual display was demonstrated.Results Totally 3 861 Chinese papers and 477 English papers were included after screening,and the annual number of papers gradually increased.The institutions that published the most Chinese and English papers were Jiangsu Kangyuan Pharmaceutical Co.,LTD.and Chinese Academy of Sciences;the authors that publish the most Chinese and English papers were Wang Zhenzhong and Chen Lin;the journals that published the most Chinese and English papers were Zhong Cao Yao and Journal of Ethnopharmacology.Keyword analysis showed higher frequency of uterine fibroids,clinical efficacy,polysaccharide and antioxidant activity.20 clusters and 36 emergent keywords were generated.Conclusion The research focus in this field is on the pharmacological components and mechanism,clinical application and data mining of Poria.

Objective:To investigate the efficacy of lightroom therapy on depressive mood and sleep problems in patients with depression, and the potential effects on physiological indices related to circadian rhythms.Methods:From October 2021 to July 2023, 54 patients with acute-phase depression hospitalized in the Mental Health Center of the First Hospital of Hebei Medical University were recruited. The participants were randomly assigned to either medication combined with the bright light therapy group (bright light group, n=36) or medication combined with the dim light therapy group (dim light group, n=18). Both groups received light therapy for 2 weeks, at 10 000 lx in the bright light group and 300 lx in the dim light group. Both groups received 30 minutes of light therapy from 7:30-8:00 a.m daily over two weeks, followed up for 1 week post-treatment. The Hamilton Depression Rating Scale (HAMD 17) was used to assess patients′ depressive symptoms, and the Pittsburgh Sleep Quality Index (PSQI) was used to assess patients′ sleep quality at baseline, at the end of every week. The 32-Item Hypomania Checklist (HCL-32) was used at the end of week 2 to assess the risk of manic switching after treatment. Daily measurements of body temperature, heart rate, and blood pressure were taken before and after light therapy, along with recording adverse events related to the therapy. Paired t- tests were used to compare changes in physiological indicators before and after treatment, and repeated measures ANOVA was applied to compare clinical symptom changes between the two groups. Results:Thirty-one and fifteen patients completed this study in the bright light and dim light groups, respectively, with no statistically significant difference in dropout rates( P>0.05). There were significant interaction effects between the time and group for HAMD 17 and PSQI score( F=5.51,4.11, both P<0.05). Both groups showed significant reductions in HAMD 17 and PSQI scores at baseline, week 1, week 2, and week 3 ( P<0.001). In the bright light group, body temperature increased significantly post-treatment on days 1-4, day 7, and day 12 (all P<0.05). Heart rate elevated on day 5 ( P<0.05).Systolic blood pressure decreased on days 4, 5, 11, and 12 compared to the pre-treatment baseline(all P<0.05). In the dim light group, systolic blood pressure increased on day 11 ( P<0.05). Diastolic blood pressure in the bright light group decreased on days 1, 5, and 6( P<0.05). No serious adverse events, vision loss, ocular structural changes occurred in either group. No hypomania or mania episodes were observed. The incidence of adverse events did not differ significantly ( P>0.05). Conclusion:Medication combined with indoor bright light is more effective than the combination of dim light for depressive symptoms and sleep problems in patients with depression. Patients receiving bright light also may exhibit a higher body temperature, accelerated heart rate, and reduced blood pressure.

Objective To analyze the research situation and trend of Ligustri Lucidi Fructus through bibliometric method;To provide reference for related research.Methods The literature on Ligustri Lucidi Fructus included in CNKI,VIP,Wanfang Data,CBM,Web of Science and PubMed from January 1,1994 to August 13,2023 was retrieved.NoteExpress 3.6.0.9155 software was used to manage and screen the literature.The publication time trend.CiteSpace 6.2.R4 and VOSviewer 1.6.18.0 software was used to analyze the authors,source journals,research institutions and keywords,and the knowledge map was drawn.Results A total of 795 Chinese papers and 175 English papers were included,and the annual number of published papers fluctuated.Capital Medical University and Hong Kong Polytechnic University published more papers in Chinese and English respectively.The authors with more papers were Liu Renhui and Wong Mansau respectively.The most published journals were Chinese Journal of Experimental Traditional Medical Formulae,Phytotherapy Research,Journal of Ethnopharmacology.The high frequency keywords included oleanolic acid,specnuezhenide,processing,antioxidant,extraction technology and rat.24 clusters and 44 emergent keywords were generated.Conclusion The research focus in this field includes the extraction and purification of oleanolic acid and specnuezhenide,the compatibility of Ligustri Lucidi Fructus with Chinese herbs such as Epimedii Folium and Polygoni Cuspidati Rhizoma et Radix,the effects and mechanism of delaying aging and treating cancer and other diseases,and cultivation and quality control.

Objective:To compare the real-world efficacy and safety profile of tenofovir amibufenamid (TMF) and tenofovir alafenamide (TAF) tablets in the treatment of patients with chronic hepatitis B (CHB).Methods:This retrospective study included patients with chronic hepatitis B who received TMF and TAF antiviral treatment at the Infectious Disease Outpatient Department of the First Affiliated Hospital of Zhengzhou University from January 2021 to December 2023. The primary and secondary outcome was to study the patient HBV DNA conversion rate (<20 IU/ml), alanine aminotransferase (ALT) normalization rate, renal function, and lipid levels of patients at 48 weeks of treatment. The comparison of data between measurement data groups was differentiated using a t-test and Mann-Whitney U test. The inter-group comparison rate in count data was performed using the χ2 test or Fisher's exact probability. Results:A total of 440 cases were enrolled, including 220 in the TMF group (63 treatment-na?ve and 157 treatment-experienced) and 220 cases in the TAF group (61 treatment-na?ve and 159 treatment-experienced). In terms of efficacy, the HBV DNA seroconversion rates in the TMF group and TAF group were 90.5% and 85.2% ( P=0.372), respectively, while the ALT normalization rates were 92.1% and 88.5% ( P=0.505), respectively, at 48 weeks of treatment. The HBV DNA-negative conversion rate for the newly treated patients was 99.4% and 98.7%, respectively ( P=1.000), while the rates of ALT normalization were 94.9% and 92.3%, respectively ( P=0.863). In terms of safety profile, the serum creatinine level was lower in the TMF group than that in the TAF group at 48 weeks of treatment [TMF group 66.5 (56.3, 78.3) μmol/L, TAF group 70.6 (60.7, 77.8) μmol/L, Z=-2.282, P=0.022]. However, there was no statistically significant difference in other renal function and tubular function related indicators between the two groups of patients ( P>0.05). The serum high-density lipoprotein levels were higher in the TMF group than those in the TAF group [TMF 1.4 (1.1, 1.6) mmol/L vs. TAF group 1.3 (1.1, 1.6) mmol/L, Z=-2.204, P=0.027] at 48 weeks of treatment. However, there was no statistically significant difference in other blood lipid indicators between the two groups of patients ( P>0.05). Conclusion:There is no statistically significant difference in efficacy and safety profiles between TMF and TAF at 48 weeks in the treatment of patients with chronic hepatitis B, and the overall safety profile is favorable.

Objective To understand the research situation of animal model of chronic heart failure(CHF)based on visualization software and bibliometrics methods,and to explore the research hotspots of animal models of CHF and guide the design of animal experiments and scientific research.Methods Literature related to animal model of CHF included in Web of Science core collection from January 1,2001 to October 10,2022 were retrieved.After reading the full text and obtaining the final included literature,VOSviewer and CiteSpace software were used to analyze the contents of institutions,journals and co-cited journals,authors and co-cited authors,keywords.Results A total of 961 papers were included,and the number of published papers increased steadily.The United States and China are the main research countries.Johns Hopkins University and Harvard University are major research institutions.The most frequently published and cited journals are AM J PHYSIOL-HEART C and CIRCULATION,etc..Schultz Harold D and Sabbah Hani N are more influential in author selection."Myocardial infarction","cardiac hypertrophy",and"rat"are the most frequent keywords,10 clusters and 18 emergent words were formed.Conclusion The research in this field is numerous,high quality but scattered.Commonly used animal models are rodents and dogs.The main modeling methods are surgery and drugs.The main pathological mechanisms are mainly myocardial hypertrophy,oxidative stress,and myocardial fibrosis.

Objective:To investigate the efficacy and safety of deep transcranial magnetic stimulation (dTMS) as an adjunctive treatment for depression.Methods:From May 2021 to June 2022, 56 patients aged 18-60 who met the diagnostic criteria in the International Classification of Diseases (ICD-10) for depression were recruited from the Mental Health Center of the First Hospital of Hebei Medical University.According to the random number table method, they were divided into dTMS combined with the drug treatment group (dTMS group, n=28), and the repetitive transcranial magnetic stimulation(rTMS) combined with the drug treatment group (rTMS group, n=28). Both groups received dTMS or rTMS based on drug therapy, 5 times weekly for 2 weeks in total. At the baseline and the end of 2nd week of treatment, the Hamilton Depression Rating Scale (HAMD 17) and Hamilton Anxiety Rating Scale (HAMA) were used to assess patients′ depression and anxiety symptoms, and the 32-Item Hypomania Checklist (HCL-32) was used to assess the risk of mania after treatment.The reductions on HAMD 17 and HAMA were the primary assessment, and the treatment response rate in HAMD 17, adverse events and the risk of switching to mania were also assessed. The efficacy was compared between the two groups by using independent samples t-test and chi-square test. Results:There was no significant difference in baseline HAMD 17 and HAMA scores between the two groups ( P>0.05). At the end of 2nd week of the treatment, The HAMD 17 reduction and the reduction rate were higher in the dTMS group than in the rTMS group( t=3.27, P=0.02; t=2.11, P=0.04). The HAMA reduction and the reduction rate showed a trend favouring the dTMS group, but the difference was insignificant ( t=1.03, P=0.31; t=0.76, P=0.45). The treatment response rates on the HAMD 17 assessment were 52.2%(12/23) and 38.5% (10/26) in the dTMS and rTMS groups, respectively. However, the difference was not statistically significant (χ 2=0.93, P=0.34). The overall incidence of adverse events was 14.3% (4/28) in the dTMS group and 7.1% (2/28) in the rTMS group, with no significant differences between the two groups (χ 2=0.19, P=0.67). All subjective discomforts during the treatment automatically resolved within three days, and no mania was reported. Conclusion:As the adjunctive treatment, both TMS protocols are safe and effective in relieving the depressive and anxious symptoms of depressive disorder, and the adjunctive dTMS seems better in treating depressive symptoms than rTMS.