Objective To investigate the expressions of long non-coding RNA antisense non-cod-ing RNA in the INK4 locus(lncRNA ANRIL)and microRNA-181b-5p(miR-181b-5p)in the serum of patients with colon cancer complicated with type 2 diabetes mellitus(T2DM)and their correlations with lymph node metastasis.Methods A total of 117 patients with colon cancer complicated with T2DM in the hospital from May 2019 to August 2022 were selected as colon cancer with T2DM group and divided into lymph node metastasis group(n=56)and no lymph node metastasis group(n=61)according to the lymph node metastasis status.Additionally,117 healthy volunteers with physical ex-aminations in the same period were selected as control group.Reverse transcription real-time quantita-tive PCR was used to detect the relative expression levels of serum lncRNA ANRIL and miR-181b-5p.Logistic regression analysis was conducted to explore the factors influencing lymph node metastasis in patients with colon cancer complicated with T2DM.Receiver operating characteristic(ROC)curve was plotted to analyze the diagnostic values of serum lncRNA ANRIL and miR-181b-5p levels for lymph node metastasis in patients with colon cancer complicated with T2DM.Pearson correlation a-nalysis was used to investigate the correlation between serum lncRNA ANRIL and miR-181b-5p ex-pression in patients with colon cancer complicated with T2DM.Results Compared with the control group,patients in the colon cancer with T2DM group had significant increased serum lncRNA ANRIL level and decreased miR-181b-5p level(P＜0.01).Compared with the no lymph node metastasis group,patients in the lymph node metastasis group had significant increased serum lncRNA ANRIL level and decreased miR-181b-5p level(P＜0.01).There were significant differences in TNM stage,degree of differentiation,and glycated hemoglobin(HbA1C)between the lymph node metas-tasis group and the no lymph node metastasis group(P＜0.05).History of diabetes,TNM stage,degree of differentiation,HbA1 C,and lncRNA ANRIL were influencing factors for lymph node me-tastasis(P＜0.05),while miR-181b-5p was a protective factor against lymph node metastasis(P＜0.05).There was a negative correlation between serum lncRNA ANRIL and miR-181b-5p levels(r=-0.440,P＜0.001).The ROC curve result showed that the areas under the curve(AUCs)for the diagnosis of lymph node metastasis by serum lncRNA ANRIL,miR-181b-5p,and their com-bination were 0.800,0.837 and 0.930 respectively.The combined diagnostic value was significant-ly higher than that of lncRNA ANRIL(Z=2.956,P=0.003)and miR-181b-5p(Z=2.117,P=0.034)alone.Conclusion Patients with colon cancer complicated with T2DM have increased ser-um lncRNA ANRIL level and decreased miR-181b-5p level,and both two indexes are correlated with lymph node metastasis.

ObjectiveTo establish a method for the analysis of oligosaccharides in Atractylodes lancea rhizome based on ultra-performance liquid chromatography tandem quadrupole-time-of-flight mass spectrometry （UPLC-Q-TOF-MS） and a method for the quantification of oligosaccharides in A. lancea rhizome based on UPLC-evaporative light scattering detector （ELSD）， and to investigate the oligosaccharide characteristics of A. lancea rhizome from different habitats. MethodUPLC-Q-TOF-MS was used to identify the oligosaccharides in A. lancea rhizome with the mobile phase of 0.1% ammonia acetonitrile solution （A）-0.1% ammonia solution （B） for gradient elution （0-0.5 min， 98%A； 0.5-2.0 min， 98%-89%A； 2.0-2.5 min， 89%-86%A； 2.5-5.5 min， 86%-80%A； 5.5-6.5 min， 80%-72%A； 6.5-9.5 min， 72%-63%A； 9.5-14.0 min， 63%-50%A； 14.0-16.0 min， 50%A； 16.0-16.5 min， 50%-98%A； 16.5-20 min， 98%A）， the column temperature of 60 ℃ and the flow rate of 0.2 mL·min^-1. Electrospray ionization （ESI） was used to collect data in negative ion mode and the detection range was m/z 50-1 500. The qualitative analysis of oligosaccharides was accomplished by retention time， relative molecular weight， primary and secondary MS information of characteristic fragment ions in combination with reference substance information. UPLC-ELSD was employed to determine the contents of nine oligosaccharides in A. lancea rhizome with the mobile phase of 0.1% ammonia acetonitrile solution （A）-0.1% ammonia solution （B） for gradient elution （0-1 min， 98%-75%A； 1-7 min， 75%-70%A； 7-18 min， 70%-55%A； 18-23 min， 55%A； 23-23.5 min， 55%-98%A； 23.5-28 min， 98%A）， the drift tube temperature of ELSD was set at 50 ℃. Principal component analysis （PCA） and orthogonal partial least squares-discriminant analysis （OPLS-DA） were used to analyze the classification and differential components between A. lancea rhizome from different habitats. ResultA total of 24 oligosaccharides， containing 11 pairs of isomers， were identified from A. lancea rhizome. Among them， compared with samples from Anhui， Chongqing， Nanjing and Shaanxi， the contents of kestose（GF2）， 1F-fructofuranosylnystose （GF4）， kestohexose （GF5）， fructo-oligosaccharide DP10 （GF9） in samples from Maoshan were statistically significant （P<0.05）， and the total mass fraction of sucrose （GF1）-GF9 reached 16.47%. The peak area ratio of fructose-fructose oligosaccharide to its isomer sucrose-fructose oligosaccharide was greater than 1 in samples from Maoshan. ConclusionThe types and contents of oligosaccharides in A. lancea rhizome vary greatly among different habitats， and the peak area ratio of fructose-fructose oligosaccharide to sucrose-fructose oligosaccharide >1 may be one of the geoherb characteristics of A. lancea rhizome， which can provide a reference for the development， utilization and quality control of this herb.

Objective:To investigate the effects of dexmedetomidine on short-term prognosis in elderly patients with gastric cancer after laparoscopic radical gastrectomy.Methods:A total of 60 elderly patients with gastric cancer admitted to Shanxi Provincial Cancer Hospital from June 2018 to September 2019 were selected as the research objects. The patients were randomly divided into the observation group and the control group according to random number method, 30 cases in each group. All patients underwent laparoscopic radical gastrectomy. The patients in the control group received routine intravenous anesthesia, and the patients in the observation group were assisted with intravenous infusion of dexmedetomidine on the basis of anesthesia in the control group. The general situation during and after surgery, hemodynamics and postoperative delirium of patients in the two groups were observed and compared.Results:The anal exhaust time and hospitalization time in the observation group was shorter than that in the control group [(72±10) h vs. (79±13) h, t = 8.384, P = 0.031; (11.0±1.7) d vs. (12.9±1.9) d, t = 7.404, P = 0.022]. While the time of abdominal drainage was (8.4±2.8) d and (8.6±1.6) d, respectively in the observation group and the control group, and the difference was not statistically significant ( t = 0.958, P = 1.092); the volume of abdominal drainage was (534±50) ml and (545± 55) ml, respectively, and the difference was not statistically significant ( t = 0.847, P = 1.202). Compared with the preoperative baseline, the mean arterial pressure (MAP) in the two groups was stable during the operation. At extubation, the MAP in the control group [(104±5) mmHg, 1 mmHg = 0.133 kPa] was higher than that in the observation group [(92±7) mmHg] ( t = 7.383, P < 0.01); in the control group, the MAP at extubation was also higher compared with the preoperative MAP [(92±9) mmHg] ( P < 0.05). The heart rate in the observation group was lower than that before surgery and the same period in the control group.The incidence rate of delirium and suspicious delirium was 3% (1/30) and 7% (2/30) in the observation group, 10% (3/30) and 13% (4/30) in the control group within 7 d after surgery, and there were statistically significant differences in delirium and suspicious delirium between the two groups ( χ2 = 9.394, 7.485, all P < 0.05). Conclusion:Dexmedetomidine can reduce the incidence of delirium in elderly patients with gastric cancer after laparoscopic radical gastrectomy, and make the hemodynamics more stable and recovery faster.

Objective:To analyze the anesthesia effect of closed-loop target-controlled infusion system in esophageal cancer surgery.Methods:A total of 78 patients with esophageal cancer who underwent surgery in Shanxi Provincial Cancer Hospital from February to October 2019 were selected and divided into observation group and control group by random number table method, with 39 cases in each group. The patients in the observation group were anesthetized by closed-loop target-controlled infusion of propofol, while the propofol target-controlled infusion concentration of patients in the control group was manually controlled by anesthesiologists. The dosage of propofol, the time from drug discontinuation to recovery, the time of stay in post-anesthesia care unit (PACU), the incidence of hypoxemia after entering PACU and the incidence of anesthesia related complications were analyzed and compared between the two groups.Results:The dosage of propofol in the observation group was lower than that in the control group [(978±163) mg vs. (1 307±278) mg], and the difference was statistically significant ( t = 9.234, P = 0.028). The time from drug discontinuation to recovery in the observation group was shorter than that in the control group [(9.7±1.2) min vs. (13.2±2.6) min], and the difference was statistically significant ( t = 9.895, P = 0.025). The time of stay in PACU in the observation group was shorter than that in the control group [(15.6±2.4) min vs. (19.5±3.0) min], and the difference was statistically significant ( t = 9.536, P = 0.026). The incidence of hypoxemia after entering the PACU in the observation group was lower than that in the control group [7.69% (3/39) vs. 23.08% (9/39)], and the difference was statistically significant ( χ2 = 10.271, P = 0.016). The total incidence of postoperative anesthesia-related complications in the observation group was lower than that in the control group [10.26% (4/39) vs. 23.08% (9/39)], and the difference was statistically significant ( χ2 = 8.353, P = 0.021). Conclusion:The application of closed-loop target-controlled infusion system can effectively reduce the dosage of propofol, shorten the recovery time of patients and the time of stay in PACU, reduce the incidence of hypoxemia after entering PACU and the incidence of postoperative anesthesia related complications, which is worthy of clinical application.

OBJECTIVE: To investigate the effect of hyperthermia on the expression of PANDAR, LncRNA-p21 and ST8SIA genes in the human lung adenocarcinoma A549 cells. METHODS: A549 cells were randomly divided into 4 groups. The A549 cells in control group were cultured at 37 ℃; the cells at experimental groups were cultured at 40, 42 or 44 ℃ respectively. The cells in these 4 groups were incubated for 1 hour, and the levels of PANDAR, LncRNA-p21 and ST8SIA genes were analyzed by real-time fluorescence quantitative polymerase chain reaction. RESULTS: In cells cultured at 40, 42 or 44 ℃ experimental groups, the relative expression of PANDAR gene was lower than that of control group(P<0.05). In cells cultured at 44 ℃ experimental group, the relative expression of PANDAR gene was lower than that of the 40 and 42 ℃ experimental groups(P<0.05). There was no significant change in the relative expression of LncRNA-p21 and ST8SIA genes among the four groups(P>0.05). CONCLUSION: Hyperthermia decrease the expression of PANDAR gene in A549 cells.

Adenomatous Polyposis Coli ; diagnosis ; genetics ; surgery ; Colonic Neoplasms ; diagnosis ; genetics ; surgery ; Family Health ; Female ; Humans ; Male ; Middle Aged ; Pedigree ; Treatment Outcome