Objective:To investigate the short-term transplantation effect of different vitrification sollutions in order to optimize the cryopreservation protocol on human ovarian tissue.Methods:Ovarian tissues came from 4 patients who were treated surgically due to gynecological diseases in Peking University Shenzhen Hospital from March 2018 to December 2019. The size of 5-10 mm×10 mm×1 mm ovarian tissues were distributed to fresh control group and four experimental groups (G1, G2, G3 and G4). After equilibrium and thawing, the frozen ovarian tissues were grafted into chorioallantoic membrane (CAM). The transplanted ovarian tissues were collected 5 d after transplantation. The morphology of follicles was assessed by HE, the expression of CD105 protein was detected by immunohistochemistry and the microvascular density was recorded. The expression of Bcl-2 and Bax proteins was detected by Western blotting.Results:There was a significantly difference in the percentage of morphologically normal primordial follicles (MNPF) among G1 [63.2% (24/38)], G2 [68.3% (28/41)], G3 [61.9% (26/42)], G4 [69.6% (32/46)] and fresh control groups [82.8% (82/99), P=0.044]. Compared with fresh control group, the percentages of MNPF were statistically lower in the treatment groups (all P<0.05). The CD105-microvascular density (MVD) in fresh control group, G1, G2, G3 and G4 groups were 6.51±1.30/mm 2, 11.10±1.62/mm 2, 13.04±1.84/mm 2, 9.11±1.09/mm 2, 11.28±1.62/mm 2, respectively. The CD105-MVD in four treatment groups after transplantation was significantly higher than that of fresh control group ( P<0.001, P<0.001, P=0.022, P<0.001), and the CD105-MVD of G1, G2 and G4 groups was higher than that of G3 group ( P=0.024, P<0.001, P=0.034). The ratio of Bcl-2/Bax in G1, G2, G3, G4 groups and fresh control group were 0.71±0.37, 0.84±0.29, 0.45±0.18, 0.84±0.29 and 0.44±0.21, respectively. The ratio of Bcl-2/Bax of G2 group was statistically higher than that of fresh control group and G3 group ( P=0.025, P=0.038). Conclusion:After 5 d of transplantation into CAM, the percentage of normal primordial follicles is lower. Acorrding to the higher vascularization and anti-apoptotic ability, G2 and G4 are the optimal vitrification protocol for human ovarian tissue cryopreservation.

Objective:To investigate the short-term transplantation effect of different vitrification sollutions in order to optimize the cryopreservation protocol on human ovarian tissue.Methods:Ovarian tissues came from 4 patients who were treated surgically due to gynecological diseases in Peking University Shenzhen Hospital from March 2018 to December 2019. The size of 5-10 mm×10 mm×1 mm ovarian tissues were distributed to fresh control group and four experimental groups (G1, G2, G3 and G4). After equilibrium and thawing, the frozen ovarian tissues were grafted into chorioallantoic membrane (CAM). The transplanted ovarian tissues were collected 5 d after transplantation. The morphology of follicles was assessed by HE, the expression of CD105 protein was detected by immunohistochemistry and the microvascular density was recorded. The expression of Bcl-2 and Bax proteins was detected by Western blotting.Results:There was a significantly difference in the percentage of morphologically normal primordial follicles (MNPF) among G1 [63.2% (24/38)], G2 [68.3% (28/41)], G3 [61.9% (26/42)], G4 [69.6% (32/46)] and fresh control groups [82.8% (82/99), P=0.044]. Compared with fresh control group, the percentages of MNPF were statistically lower in the treatment groups (all P<0.05). The CD105-microvascular density (MVD) in fresh control group, G1, G2, G3 and G4 groups were 6.51±1.30/mm 2, 11.10±1.62/mm 2, 13.04±1.84/mm 2, 9.11±1.09/mm 2, 11.28±1.62/mm 2, respectively. The CD105-MVD in four treatment groups after transplantation was significantly higher than that of fresh control group ( P<0.001, P<0.001, P=0.022, P<0.001), and the CD105-MVD of G1, G2 and G4 groups was higher than that of G3 group ( P=0.024, P<0.001, P=0.034). The ratio of Bcl-2/Bax in G1, G2, G3, G4 groups and fresh control group were 0.71±0.37, 0.84±0.29, 0.45±0.18, 0.84±0.29 and 0.44±0.21, respectively. The ratio of Bcl-2/Bax of G2 group was statistically higher than that of fresh control group and G3 group ( P=0.025, P=0.038). Conclusion:After 5 d of transplantation into CAM, the percentage of normal primordial follicles is lower. Acorrding to the higher vascularization and anti-apoptotic ability, G2 and G4 are the optimal vitrification protocol for human ovarian tissue cryopreservation.

Objective To standardize an expert consensus on pulmonary rehabilitation nursing of chronic respiratory diseases. Methods On the basis of literature review, 5 experts were interviewed and 19 experts were consulted to establish the primary and secondary themes. 10 nursing experts in this field were invited to conduct peer review on the draft consensus to modify and improve the consensus content. Results After expert consultations，14 first-level themes and 40 second-level themes were established. The value of Ca (judgment coefficient),Cs (familiarity coefficient) and Cr (authority coefficient) were 0.895,0.839 and 0.867 ,which indicated the expert consultation have high authority and credibility. The Kendall coefficients of first-round expert consultation were 0.121 and 0.151, and they were 0.205 and 0.149 in the second round (P<0.05).In the first-round expert consultation, the coefficients of variation of the first and second themes were 0.197 and 0.200,and in the second round ,they were 0.202 and 0.237. They were all less than 0.25 , indicating that the expert's judgment results were relatively consistent. Conclusion As this consensus was developed based on 19 clinical nursing professionals from across the country, it is scientific and authoritative. This consensus can not only benefit to clinical nursing practice, but also lay the foundation for the development of guideline, and it still needs further theoretical and empirical research verification.

Objective? To explore the effects of family participation in the respiratory rehabilitation of the patients with chronic obstructive pulmonary disease (COPD) in terms of their treatment compliance and outcomes. Methods? Totally 80 COPD patients admitted in The First Affiliated Hospital of Guangzhou Medical University from March 2016 to August 2017 were selected and divided into the control group (n=40) and the observation group (n=40) according to the random number table. Patients in the control group received conventional respiratory rehabilitation intervention, while the patients had their family members participated in the rehabilitation process in the observation group. Treatment compliance and effects were compared between the two groups. Results? The compliance of the observation group totaled 97.50%, while that of the control group totaled 87.50% (χ2=7.207, P＜ 0.05). After 3 months' intervention, the walking distance within 6 min of the observation group was (278.72±45.89) m; the PEF pred% was (93.46±13.47)%; and FEV1% was (87.42±14.71)%, better than those of the control group (t=4.127,7.156,3.859;P＜ 0.05). Conclusions? Family participation in respiratory rehabilitation process can improve the compliance of COPD patients significantly, thus improve the treatment effects, which is worth promoting in clinical practice.