Objective To establish and validate the reference interval for serum anti-Müllerian hormone(AMH)in healthy women of childbearing age in Shenzhen using an indirect method based on mathematical statistics.Methods Collected the AMH data for women aged 21～50 in outpatient and physical examination populations in Shenzhen Maternity and Child Health Care Hospital from 2017 to 2023.Grouping by age range of five,first performed normality tested on each group of data,and used the interquartile range method to remove outliers for non-normal data.Then established reference intervals for AMH in different age groups through indirect method(Hoffmann method)and verified them.Compared with the reference interval of the reagent instructions and analyzed the correlation be-tween serum AMH levels and age.Results The correlation coefficient between serum AMH and age in women of childbearing age was-0.642,and the difference was statistically significant(P<0.001).The distribution of AMH levels among women in six age groups was compared,and the difference was statistically significant(H=28 392.655,P<0.05),and AMH levels showed a decreasing trend with age.The reference range of serum AMH at ages 21～25,26～30,31～35,36～40,41～45 and 46～50 yesrs were 0.92～11.30,0.68～9.43,0.38～7.51,0.12～6.93,<4.42 and<1.83ng/ml,respectively,and all reference intervals in each group had been validated.Compared to the age groups provided by the manufacturer,the age groups in this study are more refined,the reference range was narrower,and the interpretation of clinical and laboratory data was more accurate.Conclusion This study used Hoffmann's indirect method for the first time to establish a reference range for AMH in women aged 21～50 years in Shenzhen.The reference interval established is more in line with the actual situation and is a simple and reliable acquisition mode,suitable for promotion and wide application in clinical labo-ratories.

Objective To establish and validate the reference interval for serum anti-Müllerian hormone(AMH)in healthy women of childbearing age in Shenzhen using an indirect method based on mathematical statistics.Methods Collected the AMH data for women aged 21～50 in outpatient and physical examination populations in Shenzhen Maternity and Child Health Care Hospital from 2017 to 2023.Grouping by age range of five,first performed normality tested on each group of data,and used the interquartile range method to remove outliers for non-normal data.Then established reference intervals for AMH in different age groups through indirect method(Hoffmann method)and verified them.Compared with the reference interval of the reagent instructions and analyzed the correlation be-tween serum AMH levels and age.Results The correlation coefficient between serum AMH and age in women of childbearing age was-0.642,and the difference was statistically significant(P<0.001).The distribution of AMH levels among women in six age groups was compared,and the difference was statistically significant(H=28 392.655,P<0.05),and AMH levels showed a decreasing trend with age.The reference range of serum AMH at ages 21～25,26～30,31～35,36～40,41～45 and 46～50 yesrs were 0.92～11.30,0.68～9.43,0.38～7.51,0.12～6.93,<4.42 and<1.83ng/ml,respectively,and all reference intervals in each group had been validated.Compared to the age groups provided by the manufacturer,the age groups in this study are more refined,the reference range was narrower,and the interpretation of clinical and laboratory data was more accurate.Conclusion This study used Hoffmann's indirect method for the first time to establish a reference range for AMH in women aged 21～50 years in Shenzhen.The reference interval established is more in line with the actual situation and is a simple and reliable acquisition mode,suitable for promotion and wide application in clinical labo-ratories.

Genomic alterations are commonly found in the signaling pathways of fibroblast growth factor receptors (FGFRs). Although there is no selective FGFR inhibitors in market, several promising inhibitors have been investigated in clinical trials, and showed encouraging efficacies in patients. By designing a hybrid between the FGFR-selectivity-enhancing motif dimethoxybenzene group and our previously identified novel scaffold, we discovered a new series of potent FGFR inhibitors, with the best one showing sub-nanomolar enzymatic activity. After several round of optimization and with the solved crystal structure, detailed structure-activity relationship was elaborated. Together with metabolic stability tests and pharmacokinetic profiling, a representative compound () was selected and tested in xenograft mouse model, and the result demonstrated that inhibitor was effective against tumors with FGFR genetic alterations, exhibiting potential for further development.

Objective To investigate the influence of clinical results of early low frequency training for acute Achilles tendon rupture after anchors repair.Methods A total of 35 patients with acute Achilles tendon rupture were treated with early low frequency training after anchors repair,including 28 males and 7 females.Aged 18 to 37 years, mean 26 years old.Duration 2h to 5d from injury to therapy.All patients palpated the discontinuity of Achilles tendon, limitative activity of ankle plantar flexion,Thompson test was positive,and MRI examination was taken.Results 35 patients were followed up for 12 to 18 months,with an average follow -up of 14.5 months.According to the Amer-Lindholm score,excellent in 30 cases,good in 5 cases,the good rate was 100%.All the wounds were healed, without infection,dehiscence,sural nerve injury and re -rupture occurred.Conclusion Early low frequency training after anchors repair for acute Achilles tendon rupture is safe and reliable,patients can receive a better functional outcome.

A series of 17 human specimens including different ages of embryos,fetuses and adults were selected for the study of developmental changes ofthe digestive epithelium ranging from the esophagus to the large intestine.Each embryo was sectioned in series and stained with H.E.,while the partsof the digestive tract of the fetus and adult were obtained segmentally andstained with H.E.,I.H.,as well as Mallory's triple staining method.Com-plete sets of sheep and pig embryo were also utilized in comparison with theearly stage of human embryo.It was found that the development of the mucous folds,villi and glands,the morphological changes of the epithelium and the differentiation of thecytoarchitecture of the epithelial cells consecutively started from the fetalmonth.Consequently,the morphological constitution of the epithelial cells ofthe digestive tract of the adult was found to be specifically differentiatedfrom that of the embryonic condition.A great variation of the differentiation of the esophageal epithelium wasobserved during the fetal period.Patches of the different kinds of epithe-lium were intermingled in the same section of the prepared slides.The oc-curence of the various kinds of epithelium is considered as an instability ofthe life span,and the typical form of the stratified squamous epitheliumwould not make its appearance until the full-term stage.No complete occlusion was observed in the esophagus and small intestineduring the embryonic period as remarked by some authors.This may be as-cribed to the individual deviation of the specimens during embryonic develop-ment.A comparative study of the digestive tracts of animals of different feedinghabits,such as monkey,rabbit,bat,weasel and domestic cat,were also madein the course of the present study in order to elucidate their histologicaldifferences.Segments of different parts of the digestive tract obtained fromeach animal were fixed and sectioned by routine procedure.Sections of eachsegment of the specimens were specifically stained with H.E.,I.H.,Mallory'striple staining method,Masson's method for argentaftin cells,and phosphc-tungstic acid and hematoxylin method for Paneth's cells.In general the morphological constitutions of the epithelium of the di-gestive tract of the corresponding parts were found to be fundamentallysimilar in the following aspects:the presence of the stratified squamousepithelium of the esophagus,the simple columnar epithelium of the stomach and intestine,the villi of the intestine,the intestinal glands and the gobletcells of the small and large intestines,and the striated border of the smallintestine,etc.The following differences were noted among the specimens studied;theparietal cells were comparatively few in number in the fundus of the monkey'sstomach and the goblet cells were absent in the rabbit's large intestine.Paneth's cells were quite common in the fundus of the intestinal glandsof man,cat and bat;but they were not found in the monkey,rabbit andweasel.The argentaffin cells were often found in scattered manner in theepithelium of the intestinal gland of the small and large intestines in man,bat and weasel,and they were also found in the bottom of the gastric glandin the monkey,but were absent in the entire digestive tract of the rabbit.