OBJECTIVE To explore the effects of Tianma xiongling zhixuan tablet on autophagy in vascular endothelial cells of rats and its potential mechanism. METHODS The rat aortic endothelial cells （RAECs） were divided into normal group， model group， blank serum group， traditional Chinese medicine （TCM） medicated serum group， autophagy blocker group， autophagy agonist group， and TCM combined with autophagy agonist group. Except for normal group， other groups were given 10 μg/mL lipopolysaccharide for 24 hours to induce RAECs inflammation injury model. Blank serum group was treated with 10% blank serum； TCM medicated serum group received 10% medicated serum derived from Tianma xiongling zhixuan tablet； autophagy blocker group was treated with 20 μmol/L of PD98059； autophagy agonist group was administered 50 μmol/L Honokiol. Lastly， the TCM combined with autophagy agonist group was given both 10% medicated serum derived from Tianma xiongling zhixuan tablet and 50 μmol/L Honokiol. The morphological characteristics of RAECs in each group were observed. The cell viability of each group， the contents of endothelin-1 （ET-1） and nitric oxide （NO）， mitochondrial reactive oxygen species， mitochondrial membrane potential， and the expression levels of PTEN-induced kinase 1 （PINK1）， Parkin， ubiquitin-binding protein （p62）， and microtubule-associated protein 1 light chain 3 （LC3） were detected. RESULTS Compared with model group， the levels of ET-1， mitochondrial reactive oxygen species， and the relative expressions of PINK1， Parkin， and LC3 proteins in the autophagy blocker group and TCM medicated serum group were decreased or down-regulated significantly （P＜0.05 or P＜0.01）； the cell viability rate （only autophagy blocker group）， NO level， mitochondrial membrane potential， and the E-mail：46164660@qq.com relative expression level of p62 protein were increased or up-regulated significantly （P＜0.05 or P＜0.01）； the pathological damage of RAECs was significantly improved， the number of cells increased significantly， and the typical paving stone-like characteristics were restored. The levels of ET-1， mitochondrial reactive oxygen species， and the relative expression levels of Parkin and LC3 proteins in the autophagy agonist group were increased or up-regulated significantly （P＜0.05 or P＜0.01）， while cell viability rate was decreased significantly （P＜0.05）， the damage of RAECs was aggravated. Compared with the autophagy agonist group， the cell viability rate and the relative expression level of p62 protein in TCM combined autophagy agonist group were increased or up-regulated significantly （P＜0.05 or P＜0.01）， while the levels of ET-1， the relative expression levels of PINK1， Parkin， and LC3 proteins were down-regulated significantly （P＜ 0.01）， the damage of RAECs was reversed to a certain extent. CONCLUSIONS Tianma xiongling zhixuan tablet protects vascular endothelial function by regulating mitochondrial autophagy， the mechanism of which may be associated with the regulation of PINK1/Parkin signaling pathway and the inhibition of mitochondrial autophagy.

Objective To study the immunomodulatory mechanism of lianqin jiedu formula based on network pharmacology meth-ods.Methods Using Troditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)and GeneCards da-tabases,the active ingredients and potential targets of lianqin jiedu formula was obtained,as well as immune regulation related targets,and the intersection targets was analyzed.The protein-protein interaction(PPI)network and compound target network was constructed,key active ingredients and core action targets were screened.Molecular docking technology was used to verify the binding activity between key active components and core targets,and the intersection targets were analyzed for Gene Ontology(GO)function and Kyoto Encyclope-dia of Genes and Genomes(KEGG)pathway enrichment using the The Database for Annotation,Visualization,and Integrated Discovery(DAVID)database.Results The Lianqin jiedu formula consists of 143 components and 164 targets,of which 130 components and 79 targets were related to immunoregulation,accounting for 90.91％and 48.17％respectively;among them,there were 13 key compounds and 18 core target genes.The top 4 core targets with the highest degree values showed good binding affinity with the top 3 key compounds.The lianqin jiedu formula was involved in immunoregulation and mainly affects 272 biological processes and 58 pathways.Conclusion The lianqin jiedu formula may exert its immunoregulation effects by targeting core targets such as tumor necrosis factor(TNF)and inter-leukin-6(IL-6)through key compounds such as quercetin,kaempferol,and β-sitosterol,affecting biological processes such as pro-tein phosphorylation,cytoplasm,and nucleus,and regulating key pathways such as metabolic pathways.This study provides a reference for the treatment of subsequent immune diseases.

Objective To study the immunomodulatory mechanism of lianqin jiedu formula based on network pharmacology meth-ods.Methods Using Troditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)and GeneCards da-tabases,the active ingredients and potential targets of lianqin jiedu formula was obtained,as well as immune regulation related targets,and the intersection targets was analyzed.The protein-protein interaction(PPI)network and compound target network was constructed,key active ingredients and core action targets were screened.Molecular docking technology was used to verify the binding activity between key active components and core targets,and the intersection targets were analyzed for Gene Ontology(GO)function and Kyoto Encyclope-dia of Genes and Genomes(KEGG)pathway enrichment using the The Database for Annotation,Visualization,and Integrated Discovery(DAVID)database.Results The Lianqin jiedu formula consists of 143 components and 164 targets,of which 130 components and 79 targets were related to immunoregulation,accounting for 90.91％and 48.17％respectively;among them,there were 13 key compounds and 18 core target genes.The top 4 core targets with the highest degree values showed good binding affinity with the top 3 key compounds.The lianqin jiedu formula was involved in immunoregulation and mainly affects 272 biological processes and 58 pathways.Conclusion The lianqin jiedu formula may exert its immunoregulation effects by targeting core targets such as tumor necrosis factor(TNF)and inter-leukin-6(IL-6)through key compounds such as quercetin,kaempferol,and β-sitosterol,affecting biological processes such as pro-tein phosphorylation,cytoplasm,and nucleus,and regulating key pathways such as metabolic pathways.This study provides a reference for the treatment of subsequent immune diseases.

Primary hepatic carcinoma (PHC) is a common malignant tumor in China.Cancer is comprehensively treated with various therapeutic regimes,including traditional Chinese medicine (TCM).TCM has been widely used to improve the quality of life,delay the time of cancer progression,and prolong the median survival time.This systematic review with meta-analysis aimed to assess the effect of TCM combined with Western therapy on primary hepatic carcinoma.A comprehensive literature search was conducted in six databases,including CNKI,VIP,Wan-Fang Database,CBM,PubMed,and Cochrane library.A total of 44 randomized controlled trials (RCTs) involving 3429 participants suffering from PHC were selected.Meta-analysis results indicated that the overall effect of TCM and Western integrative treatment on PHC was higher than that of Western intervention alone,which can postpone tumor recurrence and metastasis and prolong the overall survival time of patients with PHC.Although the obtained evidence remained weak because of the poor methodological quality of the included studies,this review provided relevant data supporting the efficacy and safety of TCM combined with Western therapies.In future research,individual RCT studies should incorporate accepted standards for trial design and reporting,proper outcome indicators according to international standards,blinding in allocation concealment,and valid follow-up periods.

Literature,achievements and other materials on acupuncture-moxibustion standardization research in recent years were collected and summarized domestically and overseas. The process of acupuncture-moxibustion standardization in China was reviewed through two phases. Also,we compared domestic acupuncture-moxibustion standardization study with the foreign one. It is considered that its domestic development is sound but challenged by world competition in its internationalization. Going forward,acupuncture-moxibustion standardization system is needed to be completed and become international. Contemporary techniques should be used including big data,artificial intelligence,internet of things,and cloud computing,etc., and its application engineering research will be improved so as to drive Chinese medicine modernization.