Objective: To investigate the association between expression levels of potassium voltage-gated channel subfamily Q member 1 (KCNQ1) gene and gout, so as to provide the basis for diagnosis, prevention and treatment of gout. Methods: A total of 179 patients diagnosed with gout at the outpatient department of Guangdong Second Provincial General Hospital were enrolled in the case group, while 179 healthy individuals matched by age (within 5 years) were selected as the control group. Demographic information, lifestyle, dietary intake and biochemical blood indicators were collected through questionnaires and laboratory tests. The relative expression levels of KCNQ1 gene mRNA were quantified using real-time fluorescence quantitative PCR. A receiver operating characteristic (ROC) curve was constructed to evaluate the diagnostic performance of KCNQ1 gene mRNA levels in distinguishing gout. The association between the relative expression level of KCNQ1 gene mRNA and gout, the interaction effects of the relative expression levels of KCNQ1 gene mRNA with dietary intake and biochemical blood indicators on gout were analyzed using a multivariate conditional logistic regression model. Results: There were 112 males (62.57%) and 67 females (37.43%) in the case group, with a mean age of (41.32±10.12) years. There were 98 males (54.75%) and 81 females (45.25%) in the control group, with a mean age of (40.24±7.62) years. The mRNA expression levels of the KCNQ1 gene were higher in the case group compared to the control group (P<0.05). The area under the ROC curve was 0.897 (95%CI: 0.865-0.928). Multivariate conditional logistic regression analysis revealed that KCNQ1 gene mRNA expression levels were positively associated with gout risk (OR=1.430, 95%CI: 1.171-1.747). Significant interactions were observed between KCNQ1 mRNA expression and seafood intake (OR=2.107, 95%CI: 1.175-3.779), KCNQ1 gene mRNA expression and uric acid levels (OR=2.373, 95%CI: 1.366-4.119), as well as between uric acid levels and seafood intake (OR=2.321, 95%CI: 1.159-4.678). Conclusion The expression levels of the KCNQ1 gene may increase the risk of gout and further increase the risk through interaction with seafood intake and uric acid levels.

ObjectiveTo elucidate the critical role of rehabilitation medical records (including electronic records) in rehabilitation medicine's clinical practice and management, comprehensively analyzed the structure, core content and data standards of rehabilitation medical records, to develop a standardized medical record data architecture and core dataset suitable for rehabilitation medicine and to explore the application of rehabilitation data in performance evaluation and payment. MethodsBased on the regulatory documents Basic Specifications for Medical Record Writing and Basic Specifications for Electronic Medical Records (Trial) issued by National Health Commission of China, and referencing the World Health Organization (WHO) Family of International Classifications (WHO-FICs) classifications, International Classification of Diseases (ICD-10/ICD-11), International Classification of Functioning, Disability and Health (ICF), and International Classification of Health Interventions (ICHI Beta-3), this study constructed the data architecture, core content and data standards for rehabilitation medical records. Furthermore, it explored the application of rehabilitation record summary sheets (home page) data in rehabilitation medical statistics and payment methods, including Diagnosis-related Groups (DRG), Diagnosis-Intervention Packet (DIP) and Case Mix Index. ResultsThis study proposed a systematic standard framework for rehabilitation medical records, covering key components such as patient demographics, rehabilitation diagnosis, functional assessment, rehabilitation treatment prescriptions, progress evaluations and discharge summaries. The research analyzed the systematic application methods and data standards of ICD-10/ICD-11, ICF and ICHI Beta-3 in the fields of medical record terminology, coding and assessment. Constructing a standardized data structure and data standards for rehabilitation medical records can significantly improve the quality of data reporting based on the medical record summary sheet, thereby enhancing the quality control of rehabilitation services, effectively supporting the optimization of rehabilitation medical insurance payment mechanisms, and contributing to the establishment of rehabilitation medical performance evaluation and payment based on DRG and DIP. ConclusionStructured rehabilitation records and data standardization are crucial tools for quality control in rehabilitation. Systematically applying the three reference classifications of the WHO-FICs, and aligning with national medical record and electronic health record specifications, facilitate the development of a standardized rehabilitation record architecture and core dataset. Standardizing rehabilitation care pathways based on the ICF methodology, and developing ICF- and ICD-11-based rehabilitation assessment tools, auxiliary diagnostic and therapeutic systems, and supporting terminology and coding systems, can effectively enhance the quality of rehabilitation records and enable interoperability and sharing of rehabilitation data with other medical data, ultimately improving the quality and safety of rehabilitation services.

Objective:To explore the care needs of primary caregivers of children with acute lymphoblastic leukemia (ALL) developed chemotherapy-induced oral mucositis (CTOM) during hospitalization and to provide a basis for improving the caregivers′ ability to care for the children and promoting their recovery.Methods:This study adopted the phenomenological design of a qualitative approach, using purposive sampling to select 10 primary caregivers of children with ALL who developed CTOM in Zhongshan People′s Hospital from September 2022 to August 2023. A semi-structured, in-depth interview was conducted to collect the data. Colaizzi analysis was used to analyze the qualitative data.Results:Among the 10 children, 5 were males and 5 were females, aged 3-13 years old; among the 10 primary caregivers, 3 were males and 7 were females, aged 31-44 years old. The primary caregivers′ care needs were categorized into four themes: wound care needs (active oral cleaning and hygiene maintenance, lack of ability to observe wounds, and anticipation of new and more effective medications); the need for knowledge of diseases (lack of knowledge related to the causes of CTOM, lack of knowledge about care and medication, seeking information from multiple sources); nutritional knowledge and dietary care needs (active supplementation awareness, limited dietary choices, lack of specific nutritional knowledge);and social network and psychological support needs (experience sharing in primary caregiver networks, psychosocial support in primary caregiver networks).Conclusions:Primary caregivers have particular problems in caring for children with ALL developed CTOM, including the need for nursing knowledge and practical skills, social support, etc. Healthcare professionals should provide the necessary support and assistance in terms of professional resources, hospital management resources and social resources.

Objective:To explore the care needs of primary caregivers of children with acute lymphoblastic leukemia (ALL) developed chemotherapy-induced oral mucositis (CTOM) during hospitalization and to provide a basis for improving the caregivers′ ability to care for the children and promoting their recovery.Methods:This study adopted the phenomenological design of a qualitative approach, using purposive sampling to select 10 primary caregivers of children with ALL who developed CTOM in Zhongshan People′s Hospital from September 2022 to August 2023. A semi-structured, in-depth interview was conducted to collect the data. Colaizzi analysis was used to analyze the qualitative data.Results:Among the 10 children, 5 were males and 5 were females, aged 3-13 years old; among the 10 primary caregivers, 3 were males and 7 were females, aged 31-44 years old. The primary caregivers′ care needs were categorized into four themes: wound care needs (active oral cleaning and hygiene maintenance, lack of ability to observe wounds, and anticipation of new and more effective medications); the need for knowledge of diseases (lack of knowledge related to the causes of CTOM, lack of knowledge about care and medication, seeking information from multiple sources); nutritional knowledge and dietary care needs (active supplementation awareness, limited dietary choices, lack of specific nutritional knowledge);and social network and psychological support needs (experience sharing in primary caregiver networks, psychosocial support in primary caregiver networks).Conclusions:Primary caregivers have particular problems in caring for children with ALL developed CTOM, including the need for nursing knowledge and practical skills, social support, etc. Healthcare professionals should provide the necessary support and assistance in terms of professional resources, hospital management resources and social resources.

Objective To analyze the thyroid hormone levels in patients with acute ischemic stroke (AIS) and non-valvular atrial fibrillation (NVAF). Methods A total of 121 patients with AIS were selected, and were divided into NVAF group (AIS patients with NVAF) and control group (AIS patients without atrial fibrillation). Serum levels of triiodothyronine (T₃), free triiodothyronine (FT₃), thyroxine (T₄), free thyroxine (FT₄) and thyroid stimulating hormone (TSH) in two groups were measured and compared. The survival of the two groups was compared. Results The serum T₃ level in the NVAF group was significantly lower than that in the control group (P < 0.05). There were no significant differences in serum FT₃, T₄, FT₄ and TSH levels between the two groups (P>0.05). There was no significant difference in survival between the two groups (P>0.05). Conclusion NVAF is associated with serum T₃ levels in AIS patients. There is no correlation between the risk of death and NVAF in AIS patients.

Objective:To retrospectively analyze the treatment status of tyrosine kinase inhibitors (TKI) in newly diagnosed patients with chronic myeloid leukemia (CML) in China.Methods:Data of chronic phase (CP) and accelerated phase (AP) CML patients diagnosed from January 2006 to December 2022 from 77 centers, ≥18 years old, and receiving initial imatinib, nilotinib, dasatinib or flumatinib-therapy within 6 months after diagnosis in China with complete data were retrospectively interrogated. The choice of initial TKI, current TKI medications, treatment switch and reasons, treatment responses and outcomes as well as the variables associated with them were analyzed.Results:6 893 patients in CP ( n=6 453, 93.6%) or AP ( n=440, 6.4%) receiving initial imatinib ( n=4 906, 71.2%), nilotinib ( n=1 157, 16.8%), dasatinib ( n=298, 4.3%) or flumatinib ( n=532, 7.2%) -therapy. With the median follow-up of 43 ( IQR 22-75) months, 1 581 (22.9%) patients switched TKI due to resistance ( n=1 055, 15.3%), intolerance ( n=248, 3.6%), pursuit of better efficacy ( n=168, 2.4%), economic or other reasons ( n=110, 1.6%). The frequency of switching TKI in AP patients was significantly-higher than that in CP patients (44.1% vs 21.5%, P<0.001), and more AP patients switched TKI due to resistance than CP patients (75.3% vs 66.1%, P=0.011). Multi-variable analyses showed that male, lower HGB concentration and ELTS intermediate/high-risk cohort were associated with lower cytogenetic and molecular responses rate and poor outcomes in CP patients; higher WBC count and initial the second-generation TKI treatment, the higher response rates; Ph + ACA at diagnosis, poor PFS. However, Sokal intermediate/high-risk cohort was only significantly-associated with lower CCyR and MMR rates and the poor PFS. Lower HGB concentration and larger spleen size were significantly-associated with the lower cytogenetic and molecular response rates in AP patients; initial the second-generation TKI treatment, the higher treatment response rates; lower PLT count, higher blasts and Ph + ACA, poorer TFS; Ph + ACA, poorer OS. Conclusion:At present, the vast majority of newly-diagnosed CML-CP or AP patients could benefit from TKI treatment in the long term with the good treatment responses and survival outcomes.

Rotavirus (RV) is one of the leading causes of acute gastroenteritis in infants and young animals worldwide. Rotavirus infection has obvious species specificity and mainly causes diarrhea in infants and young animals. The host innate responses suppress the infection and replication of rotavirus through activating multiple signaling pathways. Meanwhile, rotavirus also antagonizes the innate immune responses in various ways. This article reviewed the mechanisms of host innate immune responses to rotavirus infection and the antagonistic mechanism of rotavirus against host innate immunity with a view to providing reference for the development of therapeutic drugs and the prevention of rotavirus infection.

Objective:To investigate the application value of "four doors of the liver" approach in the laparoscopic anatomical hepatectomy.Methods:The retrospective and descriptive study was conducted. The clinicopathological data of 52 patients with liver cancer who were admitted to West China Hospital of Sichuan University from September 2018 to September 2019 were collected.Patients underwent laparoscopic anatomical hepatectomy by opening "four doors of the liver" approach. There were 36 males and 16 females, aged (53±16)years, with a range from 35 to 78 years. Observation indicators: (1) surgical situations; (2) postoperative situations; (3) follow-up and survival. Follow-up using outpatient examination or telephone interview was conducted to detect the physical situations, liver function and recurrence of liver cancer in patients up to March 2020. Measurement data with normal distribution were represented as Mean± SD, and measurment data with skewed distribution were represented as M (range). Count data were expressed as absolute numbers or percentages. Results:(1) Surgical situations: all the 52 patients underwent laparoscopic anatomical hepatectomy successfully, without perioperative death. Eight and 8 patients underwent laparoscopic left hemihepatectomy and right hemihepatectomy by opening "the first door of the liver" respectively, the operation time of which was (151±31)minutes and (190±43)minutes, the volume of blood loss was (151±20)mL and (361±51)mL. Eight and 8 patients underwent laparoscopic left inner hepatic lobotomy and left outer hepatic lobotomy by opening "the second door of the liver" respectively, the operation time of which was (171±41)minutes and (90±26)minutes, the volume of blood loss was (221±31)mL and (111±21)mL. Eight and 8 patients underwent laparoscopic right posterior hepatic lobotomy and right anterior hepatic lobotomy by opening "the third door of the liver" respectively, the operation time of which was (172±29)minutes and (220±40)minutes, the volume of blood loss was volume of (351±41)mL and (451±47)mL. Four patients underwent laparoscopic hepatic caudate lobotomy by opening "the fourth door of the liver" , the operation time of which was (246±36)minutes, the volume of blood loss was (261±31)mL. None of the 52 patients had blood transfusion. (2) Postoperative situations: all the 52 patients recovered well after surgery, with no complications such as bleeding, biliary fistula, infection or liver failure. The duration of postoperative hospital stay was (7±4)days. (3) Follow-up and survival: all the 52 patients were followed up for 6-17 months, with a median follow-up time of 10 months. At 6 months after operation, all the 52 patients achieved of Eastern Cooperative Oncology Group performance status grade 1, Child-Pugh A of liver function, without tumor recurrence or metastasis. The overall survival rate was 100%(52/52).Conclusion:It is safe and feasible to perform laparoscopic anatomical hepatectomy by the "four doors of the liver" approach.

We report a case of a newborn baby who suffered from hemolytic disease of fetus and newborn (HDFN) caused by anti-Di a. The baby presented with worsening jaundice started at three hours after birth and was transferred to Dongguan Maternal and Child Health Care Hospital. The newborn's hemoglobin (Hb) was 82 and 76 g/L at five and nine hours after birth, and the total bilirubin (TBIL) was 243.2 and 309.8 μmol/L, respectively. Blood samples of the newborn and the parents were collected for HDFN immunohematology test twelve hours after birth. They showed that the newborn and the father's blood type was A and RhDCCee, while the mother was A and RhDCcee. Direct antiglobulin test (DAT) indicateda strong positive for the newborn and negative for the parents. The reaction of the reagent to red blood cells for antibody screening with the patient's plasma, red cells eluate, and the mother's plasma were all negative, but were positive with the father's red blood cells. The newborn was recovered after treating with phototherapy, intravenous immunoglobulins and urgent blood exchange (the exchanged blood was the same ABO and RhD blood type and cross-matched). The newborn's plasma and red cells eluate were collected before blood exchange, and the mother's plasma were used to assess the red blood cells reaction, and IgG anti-Di a was identified in each sample. Di a blood typing was positive for the newborn and the father, and negative for the mother. Therefore, the newborn was diagnosed as HDFN caused by anti-Di a.

Reverse genetics approaches can directly manipulate the genome of virus at the gene level, making it possible to quickly, directly and thoroughly study the mechanisms of virus replication and pathogenesis. At present, many viruses of the family Reoviridae, such as mammalian orthoreovirus ( MRV) and bluetongue virus ( BTV) , have made great progress in basic viral research using the powerful tool of re-verse genetics. However, for members of the genus Rotavirus in the family Reoviridae, progress in the con-struction of reverse genetic systems has been slow. The remarkable reverse genetics system based on helper-viruses was established in 2006, and it was not until 2017 that the entirely plasmid-based reverse genetics system was successfully established. This paper briefly reviewed the development of reverse genetics systems for rotavirus and prospected the direction for future research in order to provide technical support for acceler-ating the basic research on mechanisms of rotavirus infection.