Objective To study the effect of Zhenpi decoction on mucosal lesions and inflammatory in patients with ulcerative colitis(UC)at remission stage.Methods A total of 90 remission-stage UC patients admitted to Wujin Hospital Affiliated to Jiangsu University(Wujin Clinical College of Xuzhou University)from August 2019 to July 2022 were enrolled.They were divided into observation group and control group by random number table,with 45 cases in each group.The control group was given mesalazine enteric-coated tablet,and the observation group was given Zhenpi decoction,both for 3 consecutive months.Colonoscopy was performed 3 months after treatment.Clinical effect,traditional Chinese medicine(TCM)syndrome score,the severity of mucosal lesions,serum levels of inflammatory factors(interleukin 6[IL-6],interleukin 23[IL-23],tumor necrosis factor-alpha[TNF-α],hypersensitive C-reactive protein[hs-CRP]),the number of intestinal flora(Bifidobacterium,Lactobacillus,Escherichia coli,Enterococcus faecalis),the incidence and recurrence rates of adverse reactions(nausea and vomiting,dizziness and headache,fatigue and belching)during medication were compared between the 2 groups.Results The total effective rate in the observation group was significantly higher than that in the control group(P<0.05).There were no significant differences in the scores of diarrhea,abdominal pain,mucous pus and blood stool,tenesmus or Baron score between the 2 groups before treatment(P>0.05).After 3-month treatment,the above indexes were significantly decreased in both groups(P<0.05),and the above scores in the observation group were significantly lower than those in the control group(P<0.05).There were no significant differences in hs-CPR,IL-6,TNF-α or IL-23 levels between the 2 groups before treatment(P>0.05).The levels of the above indexes were significantly decreased after 3-month treatment in both groups(P<0.05),and the levels of the above indexes in the observation group were significantly lower than those in the control group(P<0.05).There were no significant differences in the numbers of Bifidobacteria,Lactobacillus,Escherichia coli,or Enterococcus faecalis between the 2 groups before treatment(P>0.05).After 3-month treatment,the numbers of Bifidobacteria and Lactobacillus were significantly increased,while the numbers of Escherichia coli and Enterococcus faecalis were significantly decreased in both groups(P<0.05).After 3-month treatment,the numbers of Bifidobacteria and Lactobacillus were significantly increased,the numbers of Escherichia coli and Enterococcus faecalis were significantly decreased(P<0.05);and the observation group had more Bifidobacteria and Lactobacillus and less Escherichia coli and Enterococcus faecalis than the control group(P<0.05).The total incidence of adverse reactions and recurrence rate in the observation group were significantly lower than those in the control group(P<0.05).Conclusion Zhenpi decoction can effectively relieve intestinal mucosal lesions,reduce gastrointestinal inflammation and balance intestinal flora at UC remission stage.

ObjectiveTo observe the effect of Jianpi Yangzheng Xiaozheng decoction （JYXD） on the proliferation and stemness of the human gastric cancer （GC） cell line HGC-27 by inhibiting aerobic glycolysis， and explore the underlying mechanism. MethodMethyl thiazolyl tetrazolium （MTT） assay was employed to determine the survival rate and chemotherapy sensitivity of HGC-27 cells treated with JYXD （0.25， 0.5， 1， 2， 4， 8， 16， 32 g·L^-1）. Colony formation assay was employed to detect the effect of JYXD （2， 4， 8 g·L^-1） on the colony formation of the cells. The aerobic glycolysis level of HGC-27 cells after treatment with JYXD was measured by glucose assay kit and lactic acid assay kit. The proportion of stem cell subsets in HGC-27 cells was detected by flow cytometry. Western blot was employed to determine the expression of glycolysis-associated proteins such as lactate dehydrogenase （LDH）， hexokinase 2 （HK2）， glucose transporter 1 （GLUT1）， and pyruvate kinase isozyme M2 （PKM2）， and the expression of stemness-associated proteins such as octamer-binding transcription factor 4 （OCT4）， SRY-box transcription factor 2 （SOX2）， and Nanog. ResultJYXD （0.5， 1， 2， 4， 8， 16， 32 g·L^-1） inhibited the activity of HGC-27 cells （P<0.05， P<0.01）， with the inhibitory concentration 50（IC₅₀） of 4.83 g·L^-1， and it improved the sensitivity of HGC-27 cells to cisplatin chemotherapy. Compared with the control group， JYXD （2， 4， 8 g·L^-1） reduced the colony formation number of HGC-27 cells （P<0.01） in a concentration-dependent manner. Flow cytometry showed that compared with that in the control group， the proportion of CD44⁺CD24⁺ALDH⁺ population in the cells treated with JYXD （2， 4， 8 g·L^-1） decreased （P<0.05）. In addition， JYXD （2， 4， 8 g·L^-1） inhibited the glucose uptake and lactic acid production of HGC-27 cells. Western blot showed that compared with the control group， JYXD （2， 4， 8 g·L^-1） down-regulated the expression levels of SOX2， Nanog， OCT4， PKM2， LDH， GLUT1， and HK2 （P<0.05， P<0.01） in a concentration-dependent manner. ConclusionJYXD may inhibit the proliferation and reduce the stemness of HGC-27 cells by regulating the aerobic glycolysis.

Glenohumeral joint is the joint with the largest range of motion in human body. The characteristics of ball and socket structure make the joint unstable. The shape of glenoid (glenoid width, glenoid concave depth, glenoid twist angle and inclination angle), as well as the ligaments and tendons around the joint play an important role in maintaining joint stability. This paper describes in detail how the glenoid and its surrounding soft tissue can maintain the stability of the shoulder joint in the non limit range of motion through two mechanisms: intra articular negative pressure and concave compression effect. According to the mathematical principle, the formulas of intra-articular negative pressure and joint stability rate are summarized, which make the two concepts more convenient for clinical application. The joint stability rate reflects the concave compression effect. According to the standard computed tomography (CT) examination, we can calculate the joint stability rate of the glenohumeral joint in the up and down direction or the anteroposterior direction, so as to guide clinical practice.

Objective To explore the effects and mechanisms of periostin overexpression on migration and invasion of nasopharyngeal carcinoma ( NPC) cell line.Methods The recombinant plasmids [ pCMV-neo ( +)-periostin ] and control plasmids [pCMV-neo (+)] were transfected into 6-10B cells using lipofectamine 2000TM reagent.The expression of periostin was detected with PCR and Western blotting .Transwell chamber invasion assay was employed to assay the migration and invasion of 6-10B cells before and after transfection .A gelatin zymogram was used to detect the activity of MMP-2 and MMP-9 in cultivated supernatant of 6-10B cells before and after transfection .The expression of integrin-αvβ5 was detected by immunohistochemistry ( IHC) in 6-10Bperiostin cells, 6-10Bvector cells and 6-10B cells as well as normal nasopharyngeal mucosa ( NNM) and NPC and at the same time periostin also was detected by immumohistochemistry in NNM and NPC, and densitometry analysis using image-pro plus 6.0 software, and the correlation between periostin and integrin-αvβ5 on NPC was assayed with statistics .Results Over expression of periostin promoted cell migration and invasion.The expression levels of integrin-αvβ5 in primary NPC and 6-10Bperiostin cells were significantly higher than those in NNM and 6-10Bvector, 6-10B cells.The expression in NPC of integrin-αvβ5 showed positively correlated with the expression of periostin (r=0.682, P<0.01).Conclusion Periostin plays an important role in regulation of cell migration and invasion probably by combining with integrin-αvβ5 to improve the activities of MMPs .