A successful pregnancy in patients accepting assisted reproductive technologies needs a good endometrial receptivity. But how to assess endometrial receptivity precisely is a major issue. Evaluating endometrial receptivity through ultrasound is not reliable. The endometrial receptivity array is more accurate to find the window of implantation, but its invasiveness restricts its application. Uterine fluid changes with the menstrual cycle, which can reflect endometrial receptivity to some extent. The development of a wealth of omics technologies promote a further research of uterine fluid. This paper will review latest research progress of uterine fluid as a non-invasive medium for endometrial receptivity detection.

There are various types of chromatin remodeling factors in eukaryotes, which can be divided into four categories: SWI/SNF, ISWI, CHD and INO80, according to the differences of ATPase and other constituent protein subunits. These remodeling factors affect gene transcription regulation through changes in chromatin structure to ensure the accurate operation of various biological processes in cells. At present, the role of chromatin remodeling factors in mammalian gametogenesis and early embryonic development is not widely studied. In this paper, we will make a brief review to provide theoretical basis for further understanding of the functions about the chromatin remodeling factors in reproductive development.

There are various types of chromatin remodeling factors in eukaryotes, which can be divided into four categories: SWI/SNF, ISWI, CHD and INO80, according to the differences of ATPase and other constituent protein subunits. These remodeling factors affect gene transcription regulation through changes in chromatin structure to ensure the accurate operation of various biological processes in cells. At present, the role of chromatin remodeling factors in mammalian gametogenesis and early embryonic development is not widely studied. In this paper, we will make a brief review to provide theoretical basis for further understanding of the functions about the chromatin remodeling factors in reproductive development.

A successful pregnancy in patients accepting assisted reproductive technologies needs a good endometrial receptivity. But how to assess endometrial receptivity precisely is a major issue. Evaluating endometrial receptivity through ultrasound is not reliable. The endometrial receptivity array is more accurate to find the window of implantation, but its invasiveness restricts its application. Uterine fluid changes with the menstrual cycle, which can reflect endometrial receptivity to some extent. The development of a wealth of omics technologies promote a further research of uterine fluid. This paper will review latest research progress of uterine fluid as a non-invasive medium for endometrial receptivity detection.

Anti-Müllerian hormone (AMH), as a member of the transforming growth factor-β superfamily, changes with age in women and plays an important role in sexual differentiation. Up to now, AMH has been maturely used in the field of assisted reproduction, but it is less used in the field of non-assisted reproduction. This article briefly reviews the latest clinical application progress of AMH in the field of non-assisted reproduction, including assisted diagnosis of polycystic ovary syndrome, prediction of menopause age, assisted diagnosis and treatment of female tumors, preservation of fertility, contraception and prediction of fertility prognosis.

Anti-Müllerian hormone (AMH), as a member of the transforming growth factor-β superfamily, changes with age in women and plays an important role in sexual differentiation. Up to now, AMH has been maturely used in the field of assisted reproduction, but it is less used in the field of non-assisted reproduction. This article briefly reviews the latest clinical application progress of AMH in the field of non-assisted reproduction, including assisted diagnosis of polycystic ovary syndrome, prediction of menopause age, assisted diagnosis and treatment of female tumors, preservation of fertility, contraception and prediction of fertility prognosis.

Objective Previous studies regarding the effects of tamoxifen ( TAM) on the thin endometrium are rare .The aim of this study was to explore the effects of TAM on patients with thin endometrium undergoing frozen thawed embryo transfer ( FET ) . Methods One hundred and thirty three patients with thin endometri-um undergoing FET treatment were recruited from January 2014 to June 2016, who canceled embryo transfer ( ET) or after FET due to thin endometrium in natural cycle or hormone replacement therapy cycle .Patients were randomly divided into letrozole ( LE,n=72) group or tamoxifen (TAM,n=61) group.All of the patients started to have oral pills of Estradiol Valerate 4 mg/d on the third day of menstruating cycles , then 6mg/d on the eighth day ,after 10~12 days then having ultrasonic monitoring of endometrial thickness and blood estradiol (E2), progesterone levels, It′s called endometrial preparation for hormone replacement cycle .To letrozole, tamoxifen group,the way of endometrial preparation were as follows:patients started to have oral pills of LE 2.5mg/d,TAM 40 mg/d on the third day of menstruating cycles for 5 days, then having ultrasonic monitoring and used drug of human chorionic gonadotropic hormone ,It′s called HCG day .After the dominant follicle ovulation then took progesterone intramuscular injection 40 mg/d, oral progesterone 20 mg/d to change endometrium ,then to transplant cleavage embryos or blastocysts after taking 3 or 5 days of progesterone , It′s called embryo transplanting day .The way of TAM endometrium preparation was called TAM cycle .The general data , hormone levels and clinical out-come between two groups were analyzed . Results The serum estradiol level of LE group both on HCG and transfer day [(1193.80± 629.64)ng/L vs (2776.30±157.34)ng/L;(1195.90±820.30)ng/L vs (2129.40±1208.71) ng/L,P=0.000] were statistically lower, serum luteinizing hormone level were statistically higher than TAM group [(20.48±15.50)IU/L vs (10.59±8.34)IU/L,P<0.05];im-plantation rate of LE group were statistically lower than TAM (39.32%vs 45.83%,P=0.001).The endometrial thickness and serum E 2 and P levels in TAM cycles were significantly higher compared with those in hormone replacement therapy cycle [(8.49±1.36)mm vs (6.43±0.96)mm,P=0.018]. Conclusion Tam compared LE with patients of thin endometrium undergoing FET can increased en -dometrial thickness and improve implantation rate ,thus Providing a new solution to thin endometrium .

Objective The radiotherapy is one of main treatments for patients with malignant tumor and leads to ovarian func-tion decreasing in young women .It is very important to protect ovarian function during the process of radiotherapy .The aim of this study is to investigate the effect of melatonin ( MT) agonist on radiotherapy-induced ovarian function damage in female rats . Methods Thirty SD rats were divided into five groups randomly , which received normal saline, 200 cGy radiotherapy+normal saline, 200 cGy radiotherapy+MT(25 mg/kg), 200 cGy radiotherapy+MT(50 mg/kg), and 200 cGy radiotherapy +MT(100 mg/kg), respectively.All rats were de-capitated two weeks after radiotherapy .Levels of serum follicle-stimulating hormone (FSH) and estradiol (E2) were measured by en-zyme-linked immune sorbent assay method;the number of the primordial follicles , the growth follicles and the mature follicle were ob-served;Caspase3 activity was assayed by spectrophotometry . Results In accordance with the normal control group , RT+MT (100 mg/kg) group, RT+MT (50 mg/kg) group,RT group , serum E2 levels decreased, respectively (6.68 ±0.48, 5.73 ±1.36, 4.26 ±0.44, 2.83 ±0.51)pmol/L;FSH levels increased, respectively (0.340 ±0.011, 0.431 ±0.053, 0.479 ±0.023, 0.604 ± 0.028)ng/mL ;the total number of follicles decreased ,respectively (21.67 ±1.97, 18.00 ±2.28, 15.50 ±1.05, 11.50 ±2.43);Caspase3 activity increased,respectively (0.14 ±0.03, 0.26 ±0.06, 0.36 ±0.05, 0.50 ±0.05).Except FSH had no significant difference between RT +MT(50 mg/kg)group and RT+MT(100 mg/kg) group(P>0.05), the rest indexes had significant difference between the two groups(P<0.05). Conclusion MT can diminish radiotherapy-induced ovarian damage in female rats, it may be related to the mechanism that MT inhibits the radiotherapy-induced activation of Caspase 3.

Objective Glycodelin-A is one of glycoproteins secreted from endometrial epithelial cells , and it plays an im-portant role in embryo implantation , inhibition of reject reaction and pregnancy maintenance .Glycodelin-A will become an important potential marker in evaluating the endometrial receptivity and predicting the pregnancy outcome .The aim of this study was to investigate the correlation of glycodelin-A detected on the day of HCG and endometrial receptivity and the effects of glycodelin -A on the pregnancy outcome in the intrauterine insemination (IUI) period. Methods One hundred and seven woman patients with bilateral unobstruct-ed tubes and without endometrial lesions , endometrial polyps or intrauterine adhesions from Oct 2012 to Feb 2014 in our hospital were recruited in this study .Serum glycodelin-A in women receiving IUI on the day of HCG was measured by ELISA .Serum estradiol (E2), progesterone (P), and corpus luteum (LH) levels were measured by immunochemiluminometric assays .The condition of the endometrium was examined by transvaginalultrasonography . Results The serum glycodelin-A level on the day of HCG was higher in pregnant group [(1.47 ±0.38)ng/mL] than that in nonpregnant group ([0.62 ±0.13]ng/mL).The serum glycodelin-A level on the day of HCG was higher in endometrial thickness ≥7 mm group ([1.53 ±0.49]ng/mL) than endometrial thickness <7 mm group ([0.51 ±0.17]ng/mL). Conclusion The serum glycodelin-A level on the day of HCG may reflect endometrial receptivity to a cer-tain extent , which might have prognosis value for pregnancy following IUI .

Objective To observe the effect of pulmonary function training instrument combined with resistance abdominal breathing training on respiratory function recovery of patients with cervical spinal cord injury.Methods Sixty cases of patients with cervical spinal cord injury were randomly divided into the research group(n=30)and the control group(n=30).The control group adopted sputum drainage nursing , abdominal breathing training and the comprehensive rehabilitation treatment such as limbs functional rehabilitation training , while the research group used pulmonary function training instrument combined with resistance abdominal breathing training in addition for eight weeks .Forced vital capacity ( FVC), forced expiratory volume in one second(FEV1.0), maximal voluntary ventilation ( MVV) and the detected rate of pulmonary pathogens were observed and compared.Results The FVC, FEV1.0, MVV was respectively (68.6 ±11.3)%, (75.8 ± 9.3)%and ( 67.4 ±11.6 )% in the research group after eight weeks , and ( 60.1 ±14.9 )%, ( 64.3 ± 13.2)%and (57.8 ±13.5)%in the control group, with statistically significant differences (t=2.78,2.52, 2.61, respectively;P<0.05).The rate of pulmonary pathogens was 6.67%(2/30) in the research group and 30.00%(9/30) in the control group, and the difference was statistically significant (χ2 =21.23,P<0.05). Conclusions Pulmonary function training instrument combined with resistance abdominal breathing training can improve respiratory function recovery and reduce infection rate in patients with cervical spinal cord injury .