Objective To establish a nomogram prediction model for the risk of sarcopenia among elderly patients with type 2 diabetes mellitus(T2DM)by LASSO-Logistic regression.Methods From January 2021 to December 2023,2986 elderly T2DM patients who were admitted to The Fifth People's Hospital of Chongqing were enrolled.Based on the 2019 criteria of the Asian Sarcopenia Working Group,they were divided into a T2DM combined with sarcopenia(Sar,n=867)group and simple T2DM group(n=2119).All patients were randomly divided into a model group(n=1990)and a validation group(n=996)in a 2∶1 ratio.Another 528 patients with T2DM admitted to our hospital from January to October 2024 were selected as the external validation group.LASSO regression was used to screen variables.Logistic regression was used to analyze the influencing factors of sarcopenia.R language was used to establish a nomogram model.Receiver operating characteristic(ROC)curve and Hosmer-Lemeshow curve were used to validate the prediction model.Results The age,proportions of drinking,hypertension,hyperlipidemia,DM complications,DM duration,fasting plasma glucose(FPG),2-hour postprandial plasma glucose,hemoglobin A1c(HbA1c),triglycerides,total cholesterol,and low-density lipoprotein cholesterol in the Sar group were higher than those in the T2DM group(P<0.05).The proportion of male individuals,body mass index,and high-density lipoprotein cholesterol in the Sar group were lower than those in the T2DM group(P<0.05).LASSO regression identified 8 factors,including gender,age,drinking,hypertension,DM complications,DM duration,FPG,and HbA1c,as predictive variables for T2DM with sarcopenia.Logistic regression analysis showed that gender,age,DM duration,HbA1c,and DM complications were influencing factors for sarcopenia in elderly T2DM patients.The nomogram prediction model showed that the highest scores corresponding to DM complications,DM duration,HbA1c,age,and gender were approximately 100,70,52,43,and 30 points,respectively,with a maximum total score of approximately 295.The area under the ROC curve of the model group,validation group,and external validation group were 0.914,0.822,and 0.777,respectively.The Hosmer-Lemeshow calibration curve showed that the maximum offsets for the model group,validation group,and external validation group were 0.031,0.049,and 0.056,respectively(P=0.929,0.802,and 0.782,respectively).Conclusions Elderly T2DM patients are prone to developing sarcopenia.A nomogram model based on LASSO-Logistic analysis,which includes DM complications,DM duration,HbA1c,age,and gender,can effectively predict the risk of sarcopenia among elderly T2DM patients.

Atherosclerosis(AS)is the important pathological foundation of cardiovascular and cerebrovascular diseases.There is a high degree of consistency between AS and yin-nature carbuncles in terms of etiology,disease-location,severity of illness,pathogenesis,therapies and progression of disease.Based on the theory of yin-nature carbuncles and after the analysis of the characteristics of zang-fu organs mainly involved in AS and the intricate pathogenesis of AS,this paper put forward the use of warming-eliminating,warming-expulsing and warming-tonifying therapies together for treating AS,and established Chiqilin Powder,the experienced prescription including triplet medicinals,i.e.,Notoginseng Radix et Rhizoma,Draconis Sanguis,and Fermentum Rubrum.In the prescription,Notoginseng Radix et Rhizoma mainly has the warming-tonifying and warming-expulsing actions,and also exerts the warming-eliminating actions;Draconis Sanguis exerts eliminating,expulsing and tonifying actions simultaneously;Fermentum Rubrum mainly has the warming-tonifying and warming-eliminating actions,and also exerts the warming-expulsing actions.In clinical practice,the application of Chiqilin Powder in the treatment of AS patients with yang deficiency and phlegm blended with blood stasis syndrome has achieved remarkable therapeutic effects,which will provide reference for the clinical use of Chinese herbal medicine in the treatment of AS.

Objective:To explore the application of the suspension culture method in pancreatic cancer organoid construction.Methods:Cell suspensions obtained from 8 pancreatic cancer tissue samples at the Second Hospital of Lanzhou University between July 2023 and March 2024, were prepared by digested pancreatic cancer tumor tissues using mixed enzymes, inoculated into ultra-low adsorption culture plates for suspension culture, and when the organoids were cultured to a certain size, passaging and freezing were initiated, and their structural morphology was observed by inverted microscope. Hematoxylin-eosin (HE) staining showed that pancreatic cancer organoids were lumpy or irregularly tubular, with obvious nuclear atypia, and were remarkably similar in tissue structure to pancreatic cancer tissue.Results:Among the 8 pancreatic cancer tissue samples, pancreatic cancer organoids were successfully constructed in three patients, and HE staining showed that pancreatic cancer organoids had a high degree of structural similarity with tumor tissues. Immunohistochemistry suggested that CK7, CK19, P53, and Ki-67 were expressed in the pancreatic cancer organoids and tumor tissues of case origin in more or less the same way.Conclusion:The suspension culture method is able to construct pancreatic cancer-like organs that are approximately the same as the originating tumor tissues at the tissue level.

Objective To establish a nomogram prediction model for the risk of sarcopenia among elderly patients with type 2 diabetes mellitus(T2DM)by LASSO-Logistic regression.Methods From January 2021 to December 2023,2986 elderly T2DM patients who were admitted to The Fifth People's Hospital of Chongqing were enrolled.Based on the 2019 criteria of the Asian Sarcopenia Working Group,they were divided into a T2DM combined with sarcopenia(Sar,n=867)group and simple T2DM group(n=2119).All patients were randomly divided into a model group(n=1990)and a validation group(n=996)in a 2∶1 ratio.Another 528 patients with T2DM admitted to our hospital from January to October 2024 were selected as the external validation group.LASSO regression was used to screen variables.Logistic regression was used to analyze the influencing factors of sarcopenia.R language was used to establish a nomogram model.Receiver operating characteristic(ROC)curve and Hosmer-Lemeshow curve were used to validate the prediction model.Results The age,proportions of drinking,hypertension,hyperlipidemia,DM complications,DM duration,fasting plasma glucose(FPG),2-hour postprandial plasma glucose,hemoglobin A1c(HbA1c),triglycerides,total cholesterol,and low-density lipoprotein cholesterol in the Sar group were higher than those in the T2DM group(P<0.05).The proportion of male individuals,body mass index,and high-density lipoprotein cholesterol in the Sar group were lower than those in the T2DM group(P<0.05).LASSO regression identified 8 factors,including gender,age,drinking,hypertension,DM complications,DM duration,FPG,and HbA1c,as predictive variables for T2DM with sarcopenia.Logistic regression analysis showed that gender,age,DM duration,HbA1c,and DM complications were influencing factors for sarcopenia in elderly T2DM patients.The nomogram prediction model showed that the highest scores corresponding to DM complications,DM duration,HbA1c,age,and gender were approximately 100,70,52,43,and 30 points,respectively,with a maximum total score of approximately 295.The area under the ROC curve of the model group,validation group,and external validation group were 0.914,0.822,and 0.777,respectively.The Hosmer-Lemeshow calibration curve showed that the maximum offsets for the model group,validation group,and external validation group were 0.031,0.049,and 0.056,respectively(P=0.929,0.802,and 0.782,respectively).Conclusions Elderly T2DM patients are prone to developing sarcopenia.A nomogram model based on LASSO-Logistic analysis,which includes DM complications,DM duration,HbA1c,age,and gender,can effectively predict the risk of sarcopenia among elderly T2DM patients.

Objective To explore and study the new nationwide centralized volume-based procurement model of Chinese patent medicines(CPM),aiming further to promote the normalization and institutionalization of such procurement practices.Methods This study conducts a retrospective analysis of the entire 2023 national CPM procurement process,comparing it with the 19-province alliance procurement,the national chemical drugs procurement,and the national insulin special procurement and highlighting its similarities and differences.Results In contrast to the 19-province alliance procurement,the national CPM alliance procurement introduced a daily average cost rule to reduce the price selection deviation.It also improved and optimized the criteria for qualification,bidding unit formation principles,selection rules,resurrection mechanisms,and principles for allocating procurement volumes under agreements.A comprehensive analysis of the results from two rounds of CPM procurement indicates statistically significant differences in resurrection rates,brand substitution rates,and reduction in selected prices,with the national procurement outperforming the 19-province alliance procurement.Compared with the national chemical drugs and special insulin procurements,this procurement distinctly showcases its unique characteristics.Conclusion The new mode of the national CPM alliance procurement is innovative and has contributed valuable experiences towards the sustained advancement of CPM procurement and establishing a medical insurance payment standard system.

Objective:To explore the application of the suspension culture method in pancreatic cancer organoid construction.Methods:Cell suspensions obtained from 8 pancreatic cancer tissue samples at the Second Hospital of Lanzhou University between July 2023 and March 2024, were prepared by digested pancreatic cancer tumor tissues using mixed enzymes, inoculated into ultra-low adsorption culture plates for suspension culture, and when the organoids were cultured to a certain size, passaging and freezing were initiated, and their structural morphology was observed by inverted microscope. Hematoxylin-eosin (HE) staining showed that pancreatic cancer organoids were lumpy or irregularly tubular, with obvious nuclear atypia, and were remarkably similar in tissue structure to pancreatic cancer tissue.Results:Among the 8 pancreatic cancer tissue samples, pancreatic cancer organoids were successfully constructed in three patients, and HE staining showed that pancreatic cancer organoids had a high degree of structural similarity with tumor tissues. Immunohistochemistry suggested that CK7, CK19, P53, and Ki-67 were expressed in the pancreatic cancer organoids and tumor tissues of case origin in more or less the same way.Conclusion:The suspension culture method is able to construct pancreatic cancer-like organs that are approximately the same as the originating tumor tissues at the tissue level.

Objective To explore and study the new nationwide centralized volume-based procurement model of Chinese patent medicines(CPM),aiming further to promote the normalization and institutionalization of such procurement practices.Methods This study conducts a retrospective analysis of the entire 2023 national CPM procurement process,comparing it with the 19-province alliance procurement,the national chemical drugs procurement,and the national insulin special procurement and highlighting its similarities and differences.Results In contrast to the 19-province alliance procurement,the national CPM alliance procurement introduced a daily average cost rule to reduce the price selection deviation.It also improved and optimized the criteria for qualification,bidding unit formation principles,selection rules,resurrection mechanisms,and principles for allocating procurement volumes under agreements.A comprehensive analysis of the results from two rounds of CPM procurement indicates statistically significant differences in resurrection rates,brand substitution rates,and reduction in selected prices,with the national procurement outperforming the 19-province alliance procurement.Compared with the national chemical drugs and special insulin procurements,this procurement distinctly showcases its unique characteristics.Conclusion The new mode of the national CPM alliance procurement is innovative and has contributed valuable experiences towards the sustained advancement of CPM procurement and establishing a medical insurance payment standard system.

To isolate bone marrow mesenchymal stem cells (BM-MSCs) and establish the model of chronic kidney disease (CKD) of Wuzhishan (WZS) mini-pig, and to study the repairment effect of BM-MSCs on CKD-induced renal fibrosis in vitro.
 Methods: Density gradient method was used to isolate and culture BM-MSCs. The cells were verified by morphology, phenotype, differentiation and so on. The left partial ureteral obstruction (LPUUO) was used to establish the CKD model, which was evaluated by B-ultrasound, single-photon emission computed tomography (SPECT), HE and Masson staining. The cells were divided into 3 groups, the tissue plus BM-MSCs group, the tissue group, and the BM-MSCs group, respectively. Seven days later, the supernatants were collected to observe the changes of hepatocyte growth factor (HGF) cumulative release. HE and Masson staining was used to observe the changes of renal tissue.
 Results: The isolated BM-MSCs possessed the features as follow: fibroblast-like adherent growth; positive in CD29 and CD90 expression while negative in CD45 expression; osteogenic induction and alizarin red staining were positive; alcian blue staining were positive after chondrogenic induction. Twelve weeks after the operation of LPUUO, B-ultrasound showed the thin renal cortical with pelvis effusion; SPETCT showed the left kidney delayed filling and renal impairment. The accumulation of HGF in the tissue plus BM-MSCs group was significantly higher than that in the tissue alone group at the 1st, 5th, 6th, 7th day, respectively (P<0.05). HE staining showed the different degree of renal lesions between the tissue plus BM-MSCs+CKD group and the tissue alone group, which was aggravated with the time going. Masson staining showed that the cumulative optical density of blue-stained collagen fibers in tissue plus BM-MSCs group was significantly lower than that in the tissue group at the 5th to 7th day (P<0.05).
 Conclusion: BM-MSCs from WZS mini-pig can inhibit or delay the progress of CKD-induced renal fibrosis through autocrine HGF in vitro.
Animals ; Autocrine Communication ; physiology ; Bone Marrow Cells ; Cells, Cultured ; Fibrosis ; physiopathology ; prevention & control ; Hepatocyte Growth Factor ; metabolism ; Kidney ; drug effects ; pathology ; physiopathology ; Mesenchymal Stem Cells ; drug effects ; Renal Insufficiency, Chronic ; complications ; physiopathology ; Swine ; Swine, Miniature ; Ureteral Obstruction ; complications

Objective To study the chemical constituents from the exocarp of Juglans mandshurica.Methods The compounds were isolated by Diaion HP-20,Sephadex LH-20,MCI CHP-20,and silica gel column chromatography.Their structures were identified by physicochemical properties and spectroscopic analysis.Results Twelve compounds were elucidated as pinostrobin (Ⅰ),quercetin (Ⅱ),quercetin-3-O-?-D-glucoside (Ⅲ),chrysophanol (Ⅳ),gallic acid (Ⅴ),4-hydroxyl-cinnamic acid methyl ester (Ⅵ),vanillin (Ⅶ),caffeic acid (Ⅷ),4-hydroxylcinnamic acid (Ⅸ),?-sitosterol (Ⅹ),daucosterol (Ⅺ),and 5-hydroxyl-1,4-naphthoquinone (ⅩⅡ).Conclusion The compounds Ⅰ,Ⅳ,and Ⅵ—Ⅸ are isolated from J.mandshurica for the first time.