ObjectiveTo explore the mechanism of Huoxue Rongluo prescription （HXRLP） in repairing the blood-brain barrier （BBB） after ischemic stroke （IS）. MethodsMale C57BL/6 mice were randomly divided into sham operation （Sham） group， cerebral infarction model （MCAO） group， environmental circadian disruption with cerebral infarction model （ECD-MCAO） group， low-， medium-， and high-dose HXRLP （HXRLP-L， M， and H） groups （8.5， 17， 34 g·kg^-1·d^-1， respectively）， and positive drug butylphthalide （NBP） group （0.23 mL·d^-1）. In the Sham group， only the exposed blood vessels were isolated without suture insertion. In the other groups， the middle cerebral artery occlusion （MCAO） model of mice was prepared. In the ECD-MCAO group， HXRLP groups， and NBP group， the environmental circadian disruption （ECD） model was prepared. The mice in the Sham group， MCAO group， and ECD-MCAO group were given the same volume of soybean oil by gavage， while those in the other groups were given the corresponding drugs by gavage. Samples were collected after 7 consecutive days of administration. The mNSS score was used to evaluate the repair effect of HXRLP on neurological deficits after IS. Hematoxylin-eosin （HE） staining was used to assess the impact of HXRLP on the pathological damage of brain tissue after IS. 2，3，5-Triphenyltetrazolium chloride （TTC） staining and cerebral blood perfusion status were used to evaluate the repair effect of HXRLP on brain tissue damage after IS. Evans blue staining and transmission electron microscopy were used to evaluate the improvement effect of HXRLP on the permeability injury of BBB after IS. Immunofluorescence （IF） staining was used to observe the expression of von Willebrand Factor （vWF）， brain and muscle Arnt-like 1 （Bmal1）， and Occludin in brain tissue. Western blot was used to detect the protein expression of Bmal1， Occludin， tight junction protein （Claudin-5）， vascular endothelial growth factor （VEGF）， and angiopoietins（Ang）， and related analysis was conducted. ResultsCompared with the Sham group， the MCAO group exhibited significantly aggravated neurological deficits， cerebral infarction volume， brain pathological damage， and BBB leakage （P0.01） and significantly reduced cerebral blood perfusion （P0.01）. The expression of Bmal1， vWF， vascular endothelial growth factor A （VEGFA）， and Ang in brain tissue was significantly enhanced （P0.01）， while the expression of Occludin and Claudin-5 was significantly weakened （P0.01）. Compared with the MCAO group， the ECD-MCAO group showed significantly aggravated neurological deficits， cerebral infarction volume， and BBB leakage （P0.01）， obviously worsened brain pathological damage （P0.05）， significantly reduced cerebral blood perfusion （P0.01）， and significantly decreased expression of Bmal1， vWF， VEGFA， Ang， Occludin， and Claudin-5 in brain tissue （P0.01）. Compared with the ECD-MCAO group， the HXRLP groups of all doses presented significantly improved neurological deficits， cerebral infarction volume， brain pathological damage， and BBB leakage （P0.01）， significantly increased cerebral blood perfusion （P0.01）， and enhanced expression levels of Bmal1， vWF， VEGFA， Ang， Occludin， and Claudin-5 in brain tissue （P0.01）. ConclusionHXRLP can regulate the clock protein Bmal1 and promote the expression of VEGFA， Ang， Occludin， and Claudin-5， thereby improving BBB damage after IS.

Objective:To investigate the feasibility of the ultrasound "triangular method" in evaluating the morphology of the aortic arch in normal mid-to-late trimester fetuses，establish the gestational age-specific reference ranges for its morphological parameters，and to analyze their correlations with gestational age（GA）and cardiac valve annulus diameters.Methods:A prospective study was conducted on 317 normal singleton fetuses at 20-38 +6 weeks of gestation from the Second Xiangya Hospital of Central South University. Morphological parameters including arch length（a+b），arch width（w），arch height（h）and arch angle（θ）were measured using the "triangular method" in the aortic arch long-axis view. Arch tortuosity（a+b/w）and height-to-width ratio（h/w）were calculated. Linear regression was used to analyze correlations between arch length，width，height and GA，the reference intervals were established. Pearson correlation was applied to assess relationships between angle，tortuosity，height-to-width ratio and GA，cardiac valve annulus diameters（aortic，pulmonary，mitral，tricuspid）. Results:Aortic arch length，width and height showed strong positive correlations with GA（ r = 0.88，0.88，0.85；all P < 0.001）. The arch angle，tortuosity，and height-to-width ratio were independent of GA（all P > 0.05），with reference values of（69.2 ± 6.1）°，1.7 ± 0.1，and 0.7 ± 0.1，respectively. These parameters also showed no significant correlations with cardiac valve annulus diameters（all P > 0.05）. Conclusions:The ultrasound "triangular method" systematically quantifies fetal aortic arch morphology. The stability of arch angle，tortuosity and height-to-width ratio during mid-to-late gestation may provide a morphological benchmark for prenatal screening of aortic arch anomalies.

Objective:To investigate the feasibility of the ultrasound "triangular method" in evaluating the morphology of the aortic arch in normal mid-to-late trimester fetuses，establish the gestational age-specific reference ranges for its morphological parameters，and to analyze their correlations with gestational age（GA）and cardiac valve annulus diameters.Methods:A prospective study was conducted on 317 normal singleton fetuses at 20-38 +6 weeks of gestation from the Second Xiangya Hospital of Central South University. Morphological parameters including arch length（a+b），arch width（w），arch height（h）and arch angle（θ）were measured using the "triangular method" in the aortic arch long-axis view. Arch tortuosity（a+b/w）and height-to-width ratio（h/w）were calculated. Linear regression was used to analyze correlations between arch length，width，height and GA，the reference intervals were established. Pearson correlation was applied to assess relationships between angle，tortuosity，height-to-width ratio and GA，cardiac valve annulus diameters（aortic，pulmonary，mitral，tricuspid）. Results:Aortic arch length，width and height showed strong positive correlations with GA（ r = 0.88，0.88，0.85；all P < 0.001）. The arch angle，tortuosity，and height-to-width ratio were independent of GA（all P > 0.05），with reference values of（69.2 ± 6.1）°，1.7 ± 0.1，and 0.7 ± 0.1，respectively. These parameters also showed no significant correlations with cardiac valve annulus diameters（all P > 0.05）. Conclusions:The ultrasound "triangular method" systematically quantifies fetal aortic arch morphology. The stability of arch angle，tortuosity and height-to-width ratio during mid-to-late gestation may provide a morphological benchmark for prenatal screening of aortic arch anomalies.