[Objective]To explore the general laws of the occurrence and development of the main prescription classification methods.[Methods]Based on the classification concepts of various formulas and the era background of the corresponding periods,explore the research for the emergence and development of disease classification,function classification,species classification and stroke classification.[Results]Disease classification was the main classification approach employed in medical books of past dynasties,which originated from the need of physicians to popularize their medication experience.Function classification and species classification were mainly refined during the Ming and Qing Dynasties.The common material basis for their emergence and development was the substantial number of proven prescriptions accumulated during the course of the development of traditional Chinese medicine.Among them,the function classification was used for organizing and researching current prescriptions,while the species classification was more frequently applied in the study of classical prescriptions from Treatise on Febrile Diseases.[Conclusion]Disease classification constitutes the main thread in the development of formula classification,profoundly mirroring the evolution of the overall theory of traditional Chinese medicine,which serves as the principal vehicle for the inheritance of the medicinal application experience in traditional Chinese medicine and directly caters to clinical practice.Function classification and species classification are further derivations of the classification of diseases and syndromes in the two trajectories of current prescriptions and classical prescriptions,both leaning towards theory to a greater extent.

AIM:To investigate the mechanism of reduced tear secretion in depression-related dry eye rats based on 5-HT/TRPV4/AQP5.METHODS:Healthy SD male rats were established with chronic unpredictable mild stress(CUMS)method to establish a depression-induced dry eye model(n=8), and the control group was blank rats(n=8). The ELISA method was used to compare the 5-hydroxytryptamine(5-HT)in serum and hippocampal tissue of the two groups, and the HE sections of lacrimal gland and AQP5 immunohistochemistry were observed. Western blot and RT-PCR were used to detect the expression of 5-HT3R, TRPV4 and AQP5 in the lacrimal gland tissue of the two groups of rats.RESULTS:The tear secretion in the depression-induced group was significantly reduced(P=0.001), the serum and hippocampal 5-HT levels were significantly reduced(all P<0.05), the expression of AQP5 antibody in the lacrimal gland immunohistochemistry was significantly lower than that in the control group(P<0.001), the expression of 5-HT3R, TRPV4 and AQP5 in the lacrimal gland was significantly reduced(all P<0.05), and no obvious inflammatory cells were found in the lacrimal gland tissue sections.CONCLUSION:Depression-related dry eye may occur through a non-inflammatory 5-HT/TRPV4/AQP5 mechanism.

AIM:To investigate the mechanism of reduced tear secretion in depression-related dry eye rats based on 5-HT/TRPV4/AQP5.METHODS:Healthy SD male rats were established with chronic unpredictable mild stress(CUMS)method to establish a depression-induced dry eye model(n=8), and the control group was blank rats(n=8). The ELISA method was used to compare the 5-hydroxytryptamine(5-HT)in serum and hippocampal tissue of the two groups, and the HE sections of lacrimal gland and AQP5 immunohistochemistry were observed. Western blot and RT-PCR were used to detect the expression of 5-HT3R, TRPV4 and AQP5 in the lacrimal gland tissue of the two groups of rats.RESULTS:The tear secretion in the depression-induced group was significantly reduced(P=0.001), the serum and hippocampal 5-HT levels were significantly reduced(all P<0.05), the expression of AQP5 antibody in the lacrimal gland immunohistochemistry was significantly lower than that in the control group(P<0.001), the expression of 5-HT3R, TRPV4 and AQP5 in the lacrimal gland was significantly reduced(all P<0.05), and no obvious inflammatory cells were found in the lacrimal gland tissue sections.CONCLUSION:Depression-related dry eye may occur through a non-inflammatory 5-HT/TRPV4/AQP5 mechanism.

The incidence of age-related macular degeneration(AMD)is rising with the intensifying aging trend,be-coming a critical challenge that demands urgent solutions.Dry AMD(dAMD)accounts for approximately 80％of all AMD cases,and there is currently a lack of highly effective treatment options.In recent years,traditional Chinese medicine(TCM)has been proven to effectively treat dAMD through actions such as antioxidation,anti-apoptosis,anti-inflammation,and lipid-lowering.By reviewing domestic and international literature,this article discusses TCM monomers,extracts,and compound formulations for dAMD,analyzing their various mechanisms.Based on traditional TCM efficacy theories and in-tegrated with modern mechanisms of action,it targets the active components of TCM to elucidate the connection between effective medicinal targets of TCM and dAMD,thereby clarifying the efficacy and scientific basis of TCM monomers,com-pounds,and extracts in treating dAMD.The aim is to provide new perspectives for the prevention and clinical treatment of dAMD.

The incidence of age-related macular degeneration(AMD)is rising with the intensifying aging trend,be-coming a critical challenge that demands urgent solutions.Dry AMD(dAMD)accounts for approximately 80％of all AMD cases,and there is currently a lack of highly effective treatment options.In recent years,traditional Chinese medicine(TCM)has been proven to effectively treat dAMD through actions such as antioxidation,anti-apoptosis,anti-inflammation,and lipid-lowering.By reviewing domestic and international literature,this article discusses TCM monomers,extracts,and compound formulations for dAMD,analyzing their various mechanisms.Based on traditional TCM efficacy theories and in-tegrated with modern mechanisms of action,it targets the active components of TCM to elucidate the connection between effective medicinal targets of TCM and dAMD,thereby clarifying the efficacy and scientific basis of TCM monomers,com-pounds,and extracts in treating dAMD.The aim is to provide new perspectives for the prevention and clinical treatment of dAMD.

[Objective]To explore the general laws of the occurrence and development of the main prescription classification methods.[Methods]Based on the classification concepts of various formulas and the era background of the corresponding periods,explore the research for the emergence and development of disease classification,function classification,species classification and stroke classification.[Results]Disease classification was the main classification approach employed in medical books of past dynasties,which originated from the need of physicians to popularize their medication experience.Function classification and species classification were mainly refined during the Ming and Qing Dynasties.The common material basis for their emergence and development was the substantial number of proven prescriptions accumulated during the course of the development of traditional Chinese medicine.Among them,the function classification was used for organizing and researching current prescriptions,while the species classification was more frequently applied in the study of classical prescriptions from Treatise on Febrile Diseases.[Conclusion]Disease classification constitutes the main thread in the development of formula classification,profoundly mirroring the evolution of the overall theory of traditional Chinese medicine,which serves as the principal vehicle for the inheritance of the medicinal application experience in traditional Chinese medicine and directly caters to clinical practice.Function classification and species classification are further derivations of the classification of diseases and syndromes in the two trajectories of current prescriptions and classical prescriptions,both leaning towards theory to a greater extent.