Background: Alpha-fetoprotein (AFP) and its isoform AFP-L3 are well-established serum biomarkers for hepatocellular carcinoma (HCC), a common malignancy and a leading cause of cancer-related mortality worldwide. Current methods for measuring these biomarkers are primarily lectin-based assays including the liquid-phase binding assay (LiBA) and liquid chromatography–tandem mass spectrometry (LC-MS/MS), both of which have limitations in diagnostic sensitivity and clinical utility for samples with low AFP concentrations. We aimed to develop a lectin-independent LC-MS/MS method for quantifying fucosylated AFP proteins (AFP-Fuc%). Methods: We conducted analytical validation, including method comparisons, over 2 months. The analytical sensitivity and diagnostic performance of this method were evaluated using 525 human serum samples—235 from HCC patients and 290 from non-HCC individuals—and compared with those of LiBA, which measured AFP-L3 levels. Results: The LC-MS/MS method demonstrated acceptable within-laboratory imprecision (CVs < 17.1%) without detectable bias, carryover, or matrix effects. Our method exhibited a broader linear dynamic range (spanning five orders of magnitude) and 10-fold higher analytical sensitivity than LiBA. The diagnostic performance of our method was significantly superior to that of LiBA, particularly in patients with low AFP concentrations ( < 7 ng/mL, P < 0.001), with improved accuracy, sensitivity, and precision at a specificity of 96.2%. Conclusions The validated LC-MS/MS method demonstrated robust analytical performance and superior diagnostic accuracy over LiBA for HCC diagnosis while avoiding the inherent limitations of lectin-based assays. Our LC-MS/MS assay shows promise for early HCC detection and may contribute to enhanced patient care.

Purpose: We aimed to compare the final adult height (FAH) of male patients with central precocious puberty (CPP) after treatment with a gonadotropin-releasing hormone agonist (GnRHa). Specifically, we compared FAH with the target height (TH) and the predicted adult height (PAH) before and after GnRHa treatment to quantify height gain and identify predictive factors. Methods: We retrospectively reviewed the medical records of 92 male patients with CPP and known FAH after GnRHa treatment at the Department of Pediatrics of Severance Children’s Hospital between January 2000 and June 2024. Results: The mean duration of GnRHa treatment was 2.7±1.3 years. A significant 1.1±0.9 years narrowing was observed in the difference between bone age (BA) and chronological age (CA) during treatment (P<0.001). TH was 172.4±3.4 cm. FAH was 173.6±6.4 cm. FAH was greater than TH by 1.2±5.9 cm (P=0.047). PAH before and after treatment was 179.9±8.1 and 181.2±7.4 cm, respectively. PAH was increased by 1.3±4.9 cm (P=0.012) after treatment. As the PAH standard deviation score (SDS) before GnRHa treatment increased, FAH tended to exceed TH. In contrast, higher testosterone levels before treatment are associated with FAH falling below TH. A longer duration of treatment and taller TH are associated with an FAH SDS greater than height SDS before treatment. Conversely, a greater weight SDS, BA–CA difference, and testis size before treatment are associated with FAH SDS being less than height SDS before GnRHa treatment. Conclusion GnRHa treatment improved FAH and inhibited bone maturation in male patients with CPP.

BACKGROUND/OBJECTIVES: Dietary supplements are popular among Korean consumers in the cross-border market. This study examined the cross-border shopping trends of dietary supplements in Korea, focusing on the major health functional foods.MATERIALS/METHODS: This study analyzed the customs clearance data provided by the Korea Customs Service, spanning 2019–2021. The term ‘health functional foods,’ which are manufactured with functional ingredients specified by law, was distinguished from the broader concept of ‘dietary supplements.’ The trends in dietary supplements and the major types of health functional foods in cross-border shopping were analyzed. The top 10 most purchased health functional foods were identified and analyzed by consumer gender and age group. RESULTS: Dietary supplements constituted 19.1% of all cross-border items, with the primary consumers being women aged 30 and 40 yrs. The most popular type of health functional foods was the ‘multi-nutrient product,’ comprising 14.5% of all health functional food items.‘Protein’ was popular among men and younger age groups, while ‘probiotics’ was favored by women. In particular, 32.3% of health functional food items were related to blood health. CONCLUSION The cross-border market for dietary supplements in Korea is experiencing growth, with consumer preferences varying by gender and age. These findings can assist in the development of regulatory policies that align with market trends and consumer needs in cross-border shopping for dietary supplements.

BACKGROUND/OBJECTIVES: Dietary supplements are popular among Korean consumers in the cross-border market. This study examined the cross-border shopping trends of dietary supplements in Korea, focusing on the major health functional foods.MATERIALS/METHODS: This study analyzed the customs clearance data provided by the Korea Customs Service, spanning 2019–2021. The term ‘health functional foods,’ which are manufactured with functional ingredients specified by law, was distinguished from the broader concept of ‘dietary supplements.’ The trends in dietary supplements and the major types of health functional foods in cross-border shopping were analyzed. The top 10 most purchased health functional foods were identified and analyzed by consumer gender and age group. RESULTS: Dietary supplements constituted 19.1% of all cross-border items, with the primary consumers being women aged 30 and 40 yrs. The most popular type of health functional foods was the ‘multi-nutrient product,’ comprising 14.5% of all health functional food items.‘Protein’ was popular among men and younger age groups, while ‘probiotics’ was favored by women. In particular, 32.3% of health functional food items were related to blood health. CONCLUSION The cross-border market for dietary supplements in Korea is experiencing growth, with consumer preferences varying by gender and age. These findings can assist in the development of regulatory policies that align with market trends and consumer needs in cross-border shopping for dietary supplements.

BACKGROUND/OBJECTIVES: Dietary supplements are popular among Korean consumers in the cross-border market. This study examined the cross-border shopping trends of dietary supplements in Korea, focusing on the major health functional foods.MATERIALS/METHODS: This study analyzed the customs clearance data provided by the Korea Customs Service, spanning 2019–2021. The term ‘health functional foods,’ which are manufactured with functional ingredients specified by law, was distinguished from the broader concept of ‘dietary supplements.’ The trends in dietary supplements and the major types of health functional foods in cross-border shopping were analyzed. The top 10 most purchased health functional foods were identified and analyzed by consumer gender and age group. RESULTS: Dietary supplements constituted 19.1% of all cross-border items, with the primary consumers being women aged 30 and 40 yrs. The most popular type of health functional foods was the ‘multi-nutrient product,’ comprising 14.5% of all health functional food items.‘Protein’ was popular among men and younger age groups, while ‘probiotics’ was favored by women. In particular, 32.3% of health functional food items were related to blood health. CONCLUSION The cross-border market for dietary supplements in Korea is experiencing growth, with consumer preferences varying by gender and age. These findings can assist in the development of regulatory policies that align with market trends and consumer needs in cross-border shopping for dietary supplements.

BACKGROUND/OBJECTIVES: Dietary supplements are popular among Korean consumers in the cross-border market. This study examined the cross-border shopping trends of dietary supplements in Korea, focusing on the major health functional foods.MATERIALS/METHODS: This study analyzed the customs clearance data provided by the Korea Customs Service, spanning 2019–2021. The term ‘health functional foods,’ which are manufactured with functional ingredients specified by law, was distinguished from the broader concept of ‘dietary supplements.’ The trends in dietary supplements and the major types of health functional foods in cross-border shopping were analyzed. The top 10 most purchased health functional foods were identified and analyzed by consumer gender and age group. RESULTS: Dietary supplements constituted 19.1% of all cross-border items, with the primary consumers being women aged 30 and 40 yrs. The most popular type of health functional foods was the ‘multi-nutrient product,’ comprising 14.5% of all health functional food items.‘Protein’ was popular among men and younger age groups, while ‘probiotics’ was favored by women. In particular, 32.3% of health functional food items were related to blood health. CONCLUSION The cross-border market for dietary supplements in Korea is experiencing growth, with consumer preferences varying by gender and age. These findings can assist in the development of regulatory policies that align with market trends and consumer needs in cross-border shopping for dietary supplements.

BACKGROUND/OBJECTIVES: Dietary supplements are popular among Korean consumers in the cross-border market. This study examined the cross-border shopping trends of dietary supplements in Korea, focusing on the major health functional foods.MATERIALS/METHODS: This study analyzed the customs clearance data provided by the Korea Customs Service, spanning 2019–2021. The term ‘health functional foods,’ which are manufactured with functional ingredients specified by law, was distinguished from the broader concept of ‘dietary supplements.’ The trends in dietary supplements and the major types of health functional foods in cross-border shopping were analyzed. The top 10 most purchased health functional foods were identified and analyzed by consumer gender and age group. RESULTS: Dietary supplements constituted 19.1% of all cross-border items, with the primary consumers being women aged 30 and 40 yrs. The most popular type of health functional foods was the ‘multi-nutrient product,’ comprising 14.5% of all health functional food items.‘Protein’ was popular among men and younger age groups, while ‘probiotics’ was favored by women. In particular, 32.3% of health functional food items were related to blood health. CONCLUSION The cross-border market for dietary supplements in Korea is experiencing growth, with consumer preferences varying by gender and age. These findings can assist in the development of regulatory policies that align with market trends and consumer needs in cross-border shopping for dietary supplements.

Background: The global prevalence of hyperuricemia is steadily increasing, and reports indicate an upward trend in children and adolescents. Using data from the Korean National Health and Nutrition Examination Survey (KNHANES), this study aimed to examine the association of dietary factors with hyperuricemia among Korean children and adolescents in addition to known other risk factors. Methods: This cross-sectional study included 1,268 participants aged 10 to 18 years from the eighth KNHANES 2019–2021. Dietary information was collected using a single 24-hour recall method. The associations among serum uric acid and intake of total energy, protein, fat, sodium, and sugar were analyzed using multiple regression analysis adjusting for confounding variables (age, sex, blood pressure, estimated glomerular filtration rate [eGFR], body mass index, and hemoglobin A1c [HbA1c]). Results: From the 1,268 participants (median age, 13 years; male, 56%), 150 (11.8%) had hyperuricemia. In multiple regression analysis, higher sugar intake was independently associated with hyperuricemia (odds ratio [OR], 1.79; p = 0.01) in addition to obesity (OR, 5.5; p < 0.001), age of 13 to 15 years (OR, 2.02; p = 0.002), higher HbA1c (OR, 1.6; p = 0.04), and lower eGFR (eGFR ≥75 and <90 mL/min/1.73 m2: OR, 1.63 [p = 0.01]; eGFR <75 mL/min/1.73 m2: OR, 3.42 [p = 0.002]). Conclusion The results revealed that the increasing prevalence of hyperuricemia in Korean children and adolescents, and pubertal age, obesity, decreased kidney function, prediabetic state, and high sugar intake are associated with the risk of hyperuricemia in Korean children and adolescents.

Background: The Jr a antigen is a high-prevalence red blood cell (RBC) antigen. Reports on cases of fatal hemolytic disease of the fetus and newborn and acute hemolytic transfusion reactions suggest that antibodies against Jr a (anti-Jra ) have potential clinical significance.Identifying anti-Jra is challenging owing to a lack of commercially available antisera. We developed an alternative approach to rapidly predict the presence of anti-Jra using the TaqMan single-nucleotide polymorphism (SNP)-genotyping method. Methods: Residual peripheral blood samples from 10 patients suspected of having the anti-Jr a were collected. Two samples with confirmed Jr(a–) RBCs and anti-Jra were used to validate the TaqMan genotyping assay by comparing the genotyping results with direct sequencing. The accuracy of the assay in predicting the presence of anti-Jra was verified through crossmatching with in-house Jr(a–) O+ RBCs. Results: The TaqMan-genotyping method was validated with two Jr(a–) RBC- and anti-Jra -confirmed samples that showed concordant Jr a genotyping and direct sequencing results.Jra genotyping for the remaining samples and crossmatching the serum samples with inhouse Jr(a–) O+ RBCs showed consistent results. Conclusions We validated a rapid, simple, accurate, and cost-effective method for predicting the presence of anti-Jra using a TaqMan-based SNP-genotyping assay. Implementing this method in routine practice in clinical laboratories will assist in solving difficult problems regarding alloantibodies to high-prevalence RBC antigens and ultimately aid in providing safe and timely transfusions and proper patient care.

Background: The Jr a antigen is a high-prevalence red blood cell (RBC) antigen. Reports on cases of fatal hemolytic disease of the fetus and newborn and acute hemolytic transfusion reactions suggest that antibodies against Jr a (anti-Jra ) have potential clinical significance.Identifying anti-Jra is challenging owing to a lack of commercially available antisera. We developed an alternative approach to rapidly predict the presence of anti-Jra using the TaqMan single-nucleotide polymorphism (SNP)-genotyping method. Methods: Residual peripheral blood samples from 10 patients suspected of having the anti-Jr a were collected. Two samples with confirmed Jr(a–) RBCs and anti-Jra were used to validate the TaqMan genotyping assay by comparing the genotyping results with direct sequencing. The accuracy of the assay in predicting the presence of anti-Jra was verified through crossmatching with in-house Jr(a–) O+ RBCs. Results: The TaqMan-genotyping method was validated with two Jr(a–) RBC- and anti-Jra -confirmed samples that showed concordant Jr a genotyping and direct sequencing results.Jra genotyping for the remaining samples and crossmatching the serum samples with inhouse Jr(a–) O+ RBCs showed consistent results. Conclusions We validated a rapid, simple, accurate, and cost-effective method for predicting the presence of anti-Jra using a TaqMan-based SNP-genotyping assay. Implementing this method in routine practice in clinical laboratories will assist in solving difficult problems regarding alloantibodies to high-prevalence RBC antigens and ultimately aid in providing safe and timely transfusions and proper patient care.