Objective To study the expression of key regulators of iron metabolism,iron death inhibitor and ferririn heavy chain 1(FTH1),in bladder cancinoma and their relationship with immune invasion and prognosis.Methods Sixty patients diagnosed with bladder cancer at Jiamusi Central Hospital from January 2016 to January 2018 were chosen as the subjects of this study.Collected clinical tissue samples from patients and analyzed the protein expression of FTH1 and the infiltration abundance of three types of immune cells[CD8+T cells,CD4+T cells,natural killer(NK)cells]in bladder tumor samples through immunohistochemistry(IHC).Pearson analyzed the correlation between FTH1 and the expression of immune cell marker.Kaplan-Meier(KM)survival curve was used to analyze the relationship between FTH1 and overall survival(OS),disease free survival(DFS)in bladder cancer patients.COX proportional hazards regression model analyzed risk factors affecting the prognosis and survival of bladder cancer patients.Results The positive expression rate of FTH1 protein in bladder cancer tissue was significantly higher than that in normal adjacent tissues(37.50％vs 12.50％),with a statistically significant difference(χ2=36.391,P<0.05).The positive rate of FTH1 in cancer tissues with female gender,non papillary tumor histological subtype,and T1～T2 lesion infiltration degree,American joint committee on cancer(AJCC)stage I～Ⅱ,was lower than that in cancer tissues with male gender,papillary tumor histological subtype,T3～T4 lesion infiltration degree,and AJCC stage Ⅲ～Ⅳ,and the differences were statistically significant(χ2=4.156～13.846,all P<0.05).The five-year cumulative OS and cumulative DFS of the FTH1 high expression group were significantly lower than those of the FTH1 low expression group,and the differences were statistically significant(Log-rank χ2=25.35,33.67,all P<0.0001).The results of the multivariate COX regression analysis indicated that tumor histological subtype and high expression of FTH1 were identified as independent prognostic risk factors for bladder cancer(all P<0.01).Additionally,a positive correlation was observed between high FTH1 expression in bladder cancer and the IHC score of forkhead box protein P3+(Foxp3+)tumor-infiltrating lymphocytes(TILs)(r=0.580,P<0.05),while negative correlations were found between the IHC scores of CD8+TILs CD56+TILs,and CD4+TILs(r=-0.532,-0.533,-0.452,all P<0.05).Conclusion FTH1 as a biomarker potentiates the prediction of bladder cancer prognosis and the immune micro-environmental(IME).

Objective To study the expression of key regulators of iron metabolism,iron death inhibitor and ferririn heavy chain 1(FTH1),in bladder cancinoma and their relationship with immune invasion and prognosis.Methods Sixty patients diagnosed with bladder cancer at Jiamusi Central Hospital from January 2016 to January 2018 were chosen as the subjects of this study.Collected clinical tissue samples from patients and analyzed the protein expression of FTH1 and the infiltration abundance of three types of immune cells[CD8+T cells,CD4+T cells,natural killer(NK)cells]in bladder tumor samples through immunohistochemistry(IHC).Pearson analyzed the correlation between FTH1 and the expression of immune cell marker.Kaplan-Meier(KM)survival curve was used to analyze the relationship between FTH1 and overall survival(OS),disease free survival(DFS)in bladder cancer patients.COX proportional hazards regression model analyzed risk factors affecting the prognosis and survival of bladder cancer patients.Results The positive expression rate of FTH1 protein in bladder cancer tissue was significantly higher than that in normal adjacent tissues(37.50％vs 12.50％),with a statistically significant difference(χ2=36.391,P<0.05).The positive rate of FTH1 in cancer tissues with female gender,non papillary tumor histological subtype,and T1～T2 lesion infiltration degree,American joint committee on cancer(AJCC)stage I～Ⅱ,was lower than that in cancer tissues with male gender,papillary tumor histological subtype,T3～T4 lesion infiltration degree,and AJCC stage Ⅲ～Ⅳ,and the differences were statistically significant(χ2=4.156～13.846,all P<0.05).The five-year cumulative OS and cumulative DFS of the FTH1 high expression group were significantly lower than those of the FTH1 low expression group,and the differences were statistically significant(Log-rank χ2=25.35,33.67,all P<0.0001).The results of the multivariate COX regression analysis indicated that tumor histological subtype and high expression of FTH1 were identified as independent prognostic risk factors for bladder cancer(all P<0.01).Additionally,a positive correlation was observed between high FTH1 expression in bladder cancer and the IHC score of forkhead box protein P3+(Foxp3+)tumor-infiltrating lymphocytes(TILs)(r=0.580,P<0.05),while negative correlations were found between the IHC scores of CD8+TILs CD56+TILs,and CD4+TILs(r=-0.532,-0.533,-0.452,all P<0.05).Conclusion FTH1 as a biomarker potentiates the prediction of bladder cancer prognosis and the immune micro-environmental(IME).

Objective To investigate the multi -slice CT angiography(MSCTA)findings and explore the clinical value of cavernous transformation of the portal vein(CTPV).Methods CT and clinical materials of 29 cases CTPV were retrospectively analyzed.Results Portal vein obstruction and surrounding fine dialated portoportal collat-erals were found in all 29 cases,Gastroesophageal varices and abnormal hepatic perfusion signs were detected in 25 patients and 8 cases respectively.Pericholedochal venous plexus and cystic vein were dilated and varicose in 9 cases. Conclusion Multi -slice CT has an important clinical role in CTPV diagnosis and treatment,and it can be used to evaluate the the portal vein obstructed status,the collateral vessels,and the accompanied complications of CTPV.

ObjectiveTo evaluate the effect of N-acetylcysteine (NAC) on alleviating hepatorenal damage caused by combined chemotherapy using cisplatin-based regiments in elderly patients. MethodsAll 40 elderly patients with malignant tumors were randomly divided into AB and BA group in cross-over pattern. In AB group, combination of chemotherapy and NAC was administrated for 10 days first, and then combination of chemotherapy, carnine and vitamin C was given for 10 days. In BA group, combination of chemotherapy, earnine and vitamin C was administrated for 10 days first, and then combination of chemotherapy and NAC was given for 10 days, a cycle was 21 days. The hepatorenal damage degree was observed and the curative effect of NAC on hepatorenal damage was evaluated. ResultsThere were no differences in the levels of alanine aminotransferase(ALT), aspartate aminotransferase(AST) and serum creatinine(Cr) between pre chemotherapy and post chemotherapy in A cycle[(25.32±5.23) U/L vs. (29.18±5.43) U/L,(29.21±6.51)U/L vs. (32.37±7. 13)U/L, (89.87±19.56)Mmol/L vs. (95.22±20. 60)μmol/L,all P>0. 05] . In B cycle, the levels of ALT,AST and Cr were (56.76±5.53) U/L, (48.83±6.64)U/L and (137.33±21.16)μmol/L post chemotherapy, respectively, which were evidently higher than pre chemotherapy[(26.19 ± 5.51) U/L, (29.95±6.56) U/L and (88.66±18.27)μmol/L,respectively] (all P<0.01) . ConclusionsNAC has better preventive and therapeutic effects on hepatorenal damage caused by the chemotherapeutic drugs in elderly patients with malignant cancer.