Objective To investigate the anti-inflammatory and antioxidant effects of berberine for sepsis rats based on kelch-like ECH-associated protein 1(Keap1)/nuclear factor erythroid 2-relat-ed factor 2(Nrf2)/antioxidant response element(ARE)signaling pathway.Methods Adult health-y rats were randomly divided into normal group(20 rats)and sepsis group(80 rats).The sepsis model in rats of the sepsis group was established by cecal ligation and puncture.According to differ-ent doses of berberine administered by gavage,the sepsis group was further divided into model group(0 mg/kg,without berberine administration),low-dose group(25 mg/kg),medium-dose group(50 mg/kg)and high-dose group(100 mg/kg),with 20 rats in each group.Both the model group and the normal group were given an equal volume of pure water by gavage.Ten rats were randomly selected from each group for a survival test,and they were continuously observed for 5 days to com-pare the survival rates among the groups.Lung,kidney,and liver tissues of rats in each group were collected for hematoxylin-eosin(HE)staining,enzyme-linked immunosorbent assay(ELISA),Western blot,and real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)detection.The pathological tissue changes,serum inflammatory cytokine levels,serum biochemical indicator levels,and the relative expression levels of pathway-related proteins and their mRNAs in each group were observed and compared.Results The survival rates of rats in the medium-dose and high-dose groupsat various time points were all higher than those in the model group,and the differences were statistically significant(P＜0.05).Compared with the normal group,the model group showed in-creased levels of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),serum creatinine(SCr),blood urea nitrogen(BUN),interleukin-6(IL-6),interleukin-1 β(IL-β)and tumor necrosis factor-α(TNF-α).There were obvious pathological injuries in lung,liver,and kid-ney tissues.The relative expression levels of Nrf2 and heme oxygenase-1(HO-1)proteins and their mRNAs in lung tissues were decreased,while the relative expression levels of Keap1 mRNA and Keap1 protein were increased,and the differences were statistically significant(P＜0.05).Com-pared with the model group,the serum SCr and BUN levels in the medium-dose group,and the ser-um ALT,AST,SCr and BUN levels in the high-dose group were all decreased,with statistically sig-nificant differences(P＜0.05).Compared with the model group,the pathological injuries in lung,liver,and kidney tissues of rats in the low-dose,medium-dose,and high-dose groups were all alle-viated.The relative expression levels of Nrf2 mRNA and HO-1 mRNA as well as Nrf2 and HO-1 proteins in the lung tissues showed a dose-dependent increase,while the relative expression levels of Keap1 mRNA and Keap1 protein exhibited a dose-dependent decrease.The serum levels of IL-6 and TNF-α in the low-dose group,as well as the serum levels of IL-6,IL-1 β and TNF-α in the medium-dose and high-dose groups were all reduced(P＜0.05).Conclusion Berberine can alleviate the inflammatory response and activate the antioxidant response in sepsis rats by regulating the Keap1/Nrf2/ARE signaling pathway.

Objective To study the application effect of quality control circle(QCC)in reducing the dissatisfaction rate of physical examination clients in health management center.Methods To establish QCC,selected the health check-up popula-tion in our hospital in September-2019 and March-2020,through the questionnaire investigation and analysis,compare the dis-satisfaction of the clients before and after the quality control circle.Results After carrying out QCC activities,the dissatisfaction of physical examination clients was significantly lower than that before QCC,and the difference was statistically significant(P＜0.05).Conclusion The activities of QCC in the health management center can effectively improve the quality of the physical examination work and reduce the dissatisfaction of the customers in the physical examination.It is of great significance to the health management.

Objective To evaluate the relative safety of different ventilation methods regarding mortality and rates of complication,on neonatal respiratory distress syndrome (NRDS).Methods Network Meta-analysis was used to collect data on randomized controlled trials of pulmonary ventilation strategies in preterm infants with a mean gestational age of less than 32 weeks.Diagnostic criteria on NRDS were published in the PubMed,Cochrane,Web of Science,EBSCO,and Springer Link databases from January 1986 to June 2018.Revman 5.3 software was used to evaluate the quality of studies,based on the Cochrane quality assessment tool.Data were analyzed by Bayesian and frequency methods,using both Win BUGS 1.4.3 and STATA 13.0 software.Safety of different ventilation strategies for NRDS mortality and complications would include intraventficular hemorrhage (IVH),patent ductus arteriosus (PDA) and retinopathy of prematurity (ROP) and were evaluated.Counted data was displayed by OR and 95％CI.Results A total of 31 RCTs were included in this paper,including 5 827 preterm infants and 11 ventilation strategies.There were no statistically significant differences appearing in 11 ventilation strategies on mortality,PDA or ROE IVH results were reported in 28 studies.Compared with nasal intermittent positive pressure ventilation (NIPPV),both high-frequency oscillation ventilation (HFOV) (OR=3.33,95％ CI:1.08-16.67,P＜0.05) and synchronized intermittent mechanical ventilation (SIMV) (OR=8.22,95％CI:1.25-29.44,P＜0.05) schemes seemed to have increased the risk of IVH in preterm infants with NRDS.NIPPV appeared the optimal ventilation strategy in the rankings of cumulative probability.Results on clustering showed that NIPPV was probably the best ventilation strategy for children with NRDS after considering the orders of IVH,PDA and ROP on mortality,respectively.However,HFOV,IMV,and SIMV did not seem to be the ideal ventilated strategies.Conclusions Most of the clinical decision makers might prefer using NIPPV in the treatment of children with NRDS through mechanical ventilation systems to reduce both the incidence and death caused by IVH,PDA and ROP.It was not recommended to use HFOV,SIMV and IMV in treating NRDS with gestational less than 32 weeks.We suggested that larger numbers of multi-center RCTs ba carried out to make the above conclusions more convincing.

Objective:To determine the content of dracorhodin and curcumin in Dieda pills by UPLC .Methods:A UPLC method was adopted.The determination was performed on a Waters Acquity UPLC BEH C 18 column(100 mm ×2.1 mm,1.7 μm) with the mobile phase consisting of acetonitrile-0.05 mol· L-1 NaH2PO4(50 ∶50).The detection wavelength was 440 nm for dracorhodin and 431 nm for curcumin, the column temperature was 30℃and the flow rate was 0.1 ml· min-1 .Results:There was a good linear range of 0.001 8-0.036 4 μg(r=0.999 9)for dracorhodin and 0.000 8-0.015 6 μg(r=0.999 9) for curcumin.The average recovery for dracorhodin was 97.94%(RSD=0.89%) and that for curcumin was 98.45%(RSD=0.91%).Conclusion:The method is simple, rapid and reproducible ,which can be used for the determination of dracorhodin and curcumin in Dieda pills .

AIM: To study the action of Yuyang Powder(Herba Swertiae, Corium Elephantis, Rhizoma Bletillae, etc.) on the experimental gastric ulcer. METHODS: The rat gastric ulcer models were induced by burned acetic acid, irritabillity stimulus, ethanol injure gastric mucosa and pyloric ligation, respectively. The ulcer was obsered and the content of gastric juice, gastric acidity and pepsin were measured. RESULTS: Yuyang Powder could significantly reduce the areas of ulcers, restrain gastric ulcer, promote gastric ulcer heal, reduce gastric juice volume, restrain gastric secretion (in pyloric ligation). CONCLUSION: Yuyang Powder has the preventive and therapeutic in gastric ulcer.

AIM: To study the chemical constituents of Isodon.excia(Maxin.) Hara in Changbai Mountain. METHODS: Chromatograms column was used to separate and purify the chemical constituents.The structures were elucidated on the basis of physic-chemical properties and spectrum data. RESULTS: Two diterpenoids compounds exciain(Ⅰ) and exciain(Ⅱ),were separated and identified. CONCLUSION: The compound(Ⅰ) is obtained from this plant for the first time.

Objective: To study the optimum extracting factors of Bushenjiangu Capsule. Methods: Uniform Design was used to arrange experiments, Ursolic acid content was used to evaluate the factor levels, and the optimum extracting factors were determined according experiments and realities. The orthogonal design was used to study three factors including crude drug size extracting time and amount of water. water extracted rate and alcohol extracted substance were used to evaluate the result. Results: The optimum alcohol-extracting factors were 8 times of 70% alcohol, percatating 48 hours. The optimum water-extracting factors were 1cm or smaller the crude drug size, boiling 3 times: 3h,2h,1h, each time, the amount of water was 10,8,6 times. Conclusion: According the optimum extracting factors, the effective substance can be extracted abundantly.