Objective:To explore the mechanism of Xuanfei Dahe Decoction in treating asthmatic mice through network pharmacology and animal experiments.Methods:The active components and their targets of Xuanfei Dahe Decoction were retrieved from TCMSP, and the asthma targets were obtained by searching the GeneCards, DisGeNet, OMIM, TTD and DrugBank databases. PPI network of intersection targets was constructed using string database and Cytoscape 3.9.0 software. Go function and KEGG pathway enrichment were analyzed by metascape database. The mice were divided into a blank group of 6 mice and a model group of 30 mice according to the random number table method. The asthma model was prepared in the model group. Totally 30 successfully modeled mice were divided into the model group, the dexamethasone group, and Xuanfei Dahe Decoction low-, medium- and high-dosage groups according to the random number table method, with 6 mice in each group. On the 5th day, gavage was initiated. Xuanfei Dahe Decoction low-, medium- and high-dosage groups were respectively gavaged with Xuanfei Dahe Decoction liquid at concentrations of 6.84, 13.68 and 27.36 g/kg. The dexamethasone group was gavaged with dexamethasone acetate tablets at concentrations of 2.73 mg/kg. The blank group and the model group were gavaged with the same volume of sterile normal saline once a day for 14 consecutive days. The pathological changes of lung tissue were observed by HE staining, the level of serum IL-17 was detected by ELISA, and the expression of IL-17 in lung tissue was detected by immunohistochemistry.Results:120 asthma targets and 13 key targets were obtained from Xuanfei Dahe Decoction. Pathway enrichment analysis suggested that IL-17 signaling pathway was one of the key pathways. Compared with the model group, the levels of IL-17 in the serum of the low-dose, medium-dose and high-dose Xuanfei Dahe Decoction groups and the expression of IL-17A in the lung tissue of the medium-dose Xuanfei Dahe Decoction group decreased ( P<0.05). Conclusion:Xuanfei Dahe Decoction may treat asthma by restrain airway inflammation mediated by Th17/IL-17.

Objective To investigate the anti-inflammatory and antioxidant effects of berberine for sepsis rats based on kelch-like ECH-associated protein 1(Keap1)/nuclear factor erythroid 2-relat-ed factor 2(Nrf2)/antioxidant response element(ARE)signaling pathway.Methods Adult health-y rats were randomly divided into normal group(20 rats)and sepsis group(80 rats).The sepsis model in rats of the sepsis group was established by cecal ligation and puncture.According to differ-ent doses of berberine administered by gavage,the sepsis group was further divided into model group(0 mg/kg,without berberine administration),low-dose group(25 mg/kg),medium-dose group(50 mg/kg)and high-dose group(100 mg/kg),with 20 rats in each group.Both the model group and the normal group were given an equal volume of pure water by gavage.Ten rats were randomly selected from each group for a survival test,and they were continuously observed for 5 days to com-pare the survival rates among the groups.Lung,kidney,and liver tissues of rats in each group were collected for hematoxylin-eosin(HE)staining,enzyme-linked immunosorbent assay(ELISA),Western blot,and real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)detection.The pathological tissue changes,serum inflammatory cytokine levels,serum biochemical indicator levels,and the relative expression levels of pathway-related proteins and their mRNAs in each group were observed and compared.Results The survival rates of rats in the medium-dose and high-dose groupsat various time points were all higher than those in the model group,and the differences were statistically significant(P＜0.05).Compared with the normal group,the model group showed in-creased levels of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),serum creatinine(SCr),blood urea nitrogen(BUN),interleukin-6(IL-6),interleukin-1 β(IL-β)and tumor necrosis factor-α(TNF-α).There were obvious pathological injuries in lung,liver,and kid-ney tissues.The relative expression levels of Nrf2 and heme oxygenase-1(HO-1)proteins and their mRNAs in lung tissues were decreased,while the relative expression levels of Keap1 mRNA and Keap1 protein were increased,and the differences were statistically significant(P＜0.05).Com-pared with the model group,the serum SCr and BUN levels in the medium-dose group,and the ser-um ALT,AST,SCr and BUN levels in the high-dose group were all decreased,with statistically sig-nificant differences(P＜0.05).Compared with the model group,the pathological injuries in lung,liver,and kidney tissues of rats in the low-dose,medium-dose,and high-dose groups were all alle-viated.The relative expression levels of Nrf2 mRNA and HO-1 mRNA as well as Nrf2 and HO-1 proteins in the lung tissues showed a dose-dependent increase,while the relative expression levels of Keap1 mRNA and Keap1 protein exhibited a dose-dependent decrease.The serum levels of IL-6 and TNF-α in the low-dose group,as well as the serum levels of IL-6,IL-1 β and TNF-α in the medium-dose and high-dose groups were all reduced(P＜0.05).Conclusion Berberine can alleviate the inflammatory response and activate the antioxidant response in sepsis rats by regulating the Keap1/Nrf2/ARE signaling pathway.

[Purpose]To analyze the incidence,mortality and survival of cancers in Xiamen City in 2020.[Methods]The data relating to the incidence and mortality of cancers in Xiamen was collected from the Tumor Registration Information Direct Reporting Platform of Fujian Province to calculate the crude rate,age-standardized rate by Chinese standard population(ASRC),cumula-tive rate,truncated rate and their rank.The 5-year survival rate was analyzed by Period Analysis method.[Results]In 2020,the reported incidence rate of cancers in Xiamen residents was 350.46/105.The ASRC,the cumulative rate(0～74 years old),and the truncated rate of incidence(35～64 years old)were 275.12/105,29.70％,477.37/105,respectively.The top five cancers of in-cidence were lung cancer,female breast cancer,thyroid cancer,colorectal cancer and liver cancer.The incidence of cancers in men was higher than that in women(ASRC:280.14/105 vs 271.45/105).The curde mortality rate of cancers was 150.62/105.The ASRC,the cumulative rate(0～74 years old),and the truncated rate(35～64 years old)of mortality were 109.62/105,12.27％,160.38/105,respectively.The top five cancer deaths were lung cancer,liver cancer,colorectal cancer,esoph-agus cancer and stomach cancer.The mortality of caners in men was much higher than that in women(ASRC:155.01/105 vs 69.11/105).The 5-year observed survival rate(OSR),relative sur-vival rate(RSR)and age-standardized relative survival rate(ARSR)were 53.09％(95％C1:51.80％～54.36％),56.30％(95％CI:54.94％～57.65％)and 48.14％(95％CI:46.67％～49.64％),respectively.The 5-year ARSR was lower in men than that in women(40.23％vs 57.75％).[Conclusion]The comprehensive diagnosis and treatment level of cancers in Xiamen is in the middle and above level of the country,but the incidence rate is high.Prevention and control should be strengthened,fo-cusing on the growth trend of thyroid cancer,lung cancer,breast cancer,colorectal cancer and so on.