Acute lateral ankle sprain (ALAS) is one of the most common sport injuries, with high incidence, recurrence and disability rates. Currently, exercise rehabilitation-based non-surgical treatment is the primary management approach for ALAS. However, there remain improper practices such as excessive immobilization or uncontrolled activity, which contribute to recurrent sprains and chronic ankle instability, significantly impairing patients′ athletic function and quality of life. To standardize the non-surgical management of ALAS, improve the cure rates, and reduce the recurrence and disability rates, Chinese Sports Rehabilitation Medicine Training Project of Chinese Medical Association, Foot and Ankle Basics and Orthopedics Group, Orthopedic Branch of Chinese Medical Doctor Association, and Sports Medicine Branch of Jiangsu Medical Association organized relevant experts to formulate Expert consensus on non-surgical treatment for acute lateral ankle sprain ( version 2025), following the principles of scientific vigor, practicality, and innovation. Thirteen recommendations were proposed for standardized treatment protocols across different healing phases, aiming to provide references for standard management of ALAS and improve the therapeutic outcomes.

High mobility group box 1 (HMGB1), when released extracellularly, plays a pivotal role in the development of spinal cord synapses and exacerbates autoimmune diseases within the central nervous system. In experimental autoimmune encephalomyelitis (EAE), a condition that models multiple sclerosis, the levels of extracellular HMGB1 and interleukin-33 (IL-33) have been found to be inversely correlated. However, the mechanism by which IL-33 deficiency enhances HMGB1 release during EAE remains elusive. Our study elucidates a potential signaling pathway whereby the absence of IL-33 leads to increased binding of P300/CBP-associated factor with HMGB1 in the nuclei of astrocytes, upregulating HMGB1 acetylation and promoting its release from astrocyte nuclei in the spinal cord of EAE mice. Conversely, the addition of IL-33 counteracts the TNF-α-induced increase in HMGB1 and acetylated HMGB1 levels in primary astrocytes. These findings underscore the potential of IL-33-associated signaling pathways as a therapeutic target for EAE treatment.
Animals ; Encephalomyelitis, Autoimmune, Experimental/metabolism* ; Astrocytes/metabolism* ; Interleukin-33/metabolism* ; HMGB1 Protein/metabolism* ; Acetylation ; Mice, Knockout ; Mice, Inbred C57BL ; p300-CBP Transcription Factors/metabolism* ; Mice ; Spinal Cord/metabolism* ; Cells, Cultured ; Female ; Signal Transduction

Acute lateral ankle sprain (ALAS) is one of the most common sport injuries, with high incidence, recurrence and disability rates. Currently, exercise rehabilitation-based non-surgical treatment is the primary management approach for ALAS. However, there remain improper practices such as excessive immobilization or uncontrolled activity, which contribute to recurrent sprains and chronic ankle instability, significantly impairing patients′ athletic function and quality of life. To standardize the non-surgical management of ALAS, improve the cure rates, and reduce the recurrence and disability rates, Chinese Sports Rehabilitation Medicine Training Project of Chinese Medical Association, Foot and Ankle Basics and Orthopedics Group, Orthopedic Branch of Chinese Medical Doctor Association, and Sports Medicine Branch of Jiangsu Medical Association organized relevant experts to formulate Expert consensus on non-surgical treatment for acute lateral ankle sprain ( version 2025), following the principles of scientific vigor, practicality, and innovation. Thirteen recommendations were proposed for standardized treatment protocols across different healing phases, aiming to provide references for standard management of ALAS and improve the therapeutic outcomes.

Objective To establish a quality indicator system of interventional nursing.Methods Taking Donabedian quality structure model as the theoretical basis and through literature analysis,expert meeting and summarizing the clinical problems,the first draft about interventional nursing care quality indicators was composed,which included 3 first-level indicators,12 second-level indicators and 55 third-level indicators.A total of 27 experts on interventional nursing and nursing administration were selected from 25 grade-3A level hospitals in 13 provinces and cities of China.The Delphi method was used to conduct two rounds of expert correspondence consultation,and the quality indicator system of interventional nursing was established.Results Finally,a quality indicator system of interventional nursing,which included 3 first-level indicators,7 second-level indicators and 29 third-level indicators,was formulated.In both the two rounds of expert letter inquiry,the positive coefficient and authority coefficient were 100％and 0.95 respectively.The coefficient of variation of each index in the first and the second round of expert letter inquiry was 0-0.25 and 0-0.21 respectively.In the first round of expert letter inquiry,the Kendall harmony coefficients of the first-level,second-level and third-level indexes were 0.672,0.271 and 0.341 respectively,while in the second round of expert letter inquiry,the Kendall harmony coefficients of the first-level,second-level and third-level indexes were 0.462,0.475 and 0.330 respectively,the differences were statistically significant(all P＜0.01).Conclusion The quality indicator system of interventional nursing established in this study carries a relatively high reliability and scientific nature,and it can provide reference for evaluating and monitoring the safety and quality control in interventional nursing.

Objective:To explore the clinical outcomes of locking compression plating (LCP) in the treatment of periprosthetic fracture (PPF) of the distal femur in the aged patients.Methods:A retrospective study was performed to analyze the 31 aged patients who had been treated at Department of Orthopedic Surgery, The Affiliated Hospital to Nantong University for PPF of the distal femur with LCP between June 2012 and May 2023. There were 27 females and 4 males with an age of (80.2±6.1) years. According to the Unified Classification System (UCS), 18 PPFs were classified as type Ⅴ.3B1 and 6 PPFs as type Ⅴ.3B2 after total knee arthroplasty and 7 PPFs as type Ⅳ.3C after total hip arthroplasty. The patients were fixated with a lateral single plate in 25 cases, and with lateral and medial dual plates in 6 cases. The surgical time, intraoperative blood loss, hospitalization time, postoperative weight-bearing time, fracture healing time, and knee joint function and complications during follow-up were recorded.Results:For the 25 patients undergoing fixation with a lateral single plate, the surgical time was (58.7±7.9) minutes, the intraoperative blood loss (78.0±15.1) mL, the hospitalization time (6.9±1.6) days, the postoperative weight-bearing time (5.9±1.4) days, and the follow-up time 37 (15, 51) months. For the 6 patients undergoing fixation with lateral and medial dual plates, the surgical time was (186.6±9.8) minutes, the intraoperative blood loss (1,256.7±231.2) mL, the hospitalization time (17.8±3.3) days, the postoperative weight-bearing time (3.6±0.6) days, and the follow-up time 17 (16, 21) months. The fracture healing time was (14.9±2.0) and (18.7±2.6) weeks, respectively, for patients fixed with single and double steel plates. By the scoring criteria of the American Hospital for Special Surgery (HSS), the knee joint function was evaluated at the last follow-up as excellent in 10 cases and as good in 15 cases for the 25 patients undergoing fixation with a lateral single plate, and as good for all the 6 patients undergoing fixation with lateral and medial dual plates. No patient experienced such complications as incision infection, bone nonunion, or internal fixation failure during the follow-up period.Conclusions:LCP fixation can achieve satisfactory outcomes in the treatment of PPF of the distal femur in the aged patients. As fixation with a single lateral femoral plate is suitable for most of the aged patients with PPF of the distal femur, it can be used as the first choice. Fixation with dual plates can provide stronger stability, but its indications should be strictly controlled.

Objective The efficacy of Zang Bi Formula(ZBF)in cardiac complications of rheumatoid arthritis(RA)was observed through animal experiments.Ultra-high performance liquid chromatography tandem time-of-flight mass spectrometry(UHPLC-TOF-MS)and network pharmacology was used to analyze the active ingredients,targets,and pathways of ZBF for the treatment of cardiac hypertrophy,and molecular docking was applied to verify the binding ability of the ingredients to pathway-related proteins.Methods TNF transgenic(TNF-Tg)mice were used as RA and RA-complexed cardiac hypertrophy models,and the left ventricular hypertrophy of mice was observed by echocardiography after 8 weeks of continuous gavage with ZBF,and WGA staining,HE staining,and Masson staining were used to observe and analyze the pathological changes of cardiac tissues.The chemical composition of ZBF was identified by UHPLC-TOF-MS technique.The public database was then used to screen the active ingredients and component targets and disease targets,construct interaction networks,and analyze the bioconcentration data as well as the docking ability of the active ingredients and proteins.Results In animal experiments,ZBF reduced cardiac weight index and cardiac weight tibia length ratio in TNF-Tg mice(P＜0.05,P＜0.01),attenuated cardiac hypertrophy(P＜0.001)and cardiomyocyte hypertrophy(P＜0.01),and alleviated inflammatory infiltration and fibrosis in the heart(P＜0.0001).A total of 24 compounds were identified from ZBF by UHPLC-TOF-MS.A total of 105 active ingredients of ZBF were screened by network pharmacological analysis,and 187 potential targets of ZBF for cardiac hypertrophy were constructed,and the enriched pathways with significance included PI3K-AKT signaling pathway,TNF signaling pathway,and MAPK signaling pathway.Conclusion ZBF attenuated myocardial hypertrophy and cardiomyocyte hypertrophy in TNF-Tg mice and alleviated inflammatory infiltration and fibrosis in the heart,and ZBF may be a potential drug for the treatment of RA and its cardiac complications in the clinic.

Objective The efficacy of Zang Bi Formula(ZBF)in cardiac complications of rheumatoid arthritis(RA)was observed through animal experiments.Ultra-high performance liquid chromatography tandem time-of-flight mass spectrometry(UHPLC-TOF-MS)and network pharmacology was used to analyze the active ingredients,targets,and pathways of ZBF for the treatment of cardiac hypertrophy,and molecular docking was applied to verify the binding ability of the ingredients to pathway-related proteins.Methods TNF transgenic(TNF-Tg)mice were used as RA and RA-complexed cardiac hypertrophy models,and the left ventricular hypertrophy of mice was observed by echocardiography after 8 weeks of continuous gavage with ZBF,and WGA staining,HE staining,and Masson staining were used to observe and analyze the pathological changes of cardiac tissues.The chemical composition of ZBF was identified by UHPLC-TOF-MS technique.The public database was then used to screen the active ingredients and component targets and disease targets,construct interaction networks,and analyze the bioconcentration data as well as the docking ability of the active ingredients and proteins.Results In animal experiments,ZBF reduced cardiac weight index and cardiac weight tibia length ratio in TNF-Tg mice(P＜0.05,P＜0.01),attenuated cardiac hypertrophy(P＜0.001)and cardiomyocyte hypertrophy(P＜0.01),and alleviated inflammatory infiltration and fibrosis in the heart(P＜0.0001).A total of 24 compounds were identified from ZBF by UHPLC-TOF-MS.A total of 105 active ingredients of ZBF were screened by network pharmacological analysis,and 187 potential targets of ZBF for cardiac hypertrophy were constructed,and the enriched pathways with significance included PI3K-AKT signaling pathway,TNF signaling pathway,and MAPK signaling pathway.Conclusion ZBF attenuated myocardial hypertrophy and cardiomyocyte hypertrophy in TNF-Tg mice and alleviated inflammatory infiltration and fibrosis in the heart,and ZBF may be a potential drug for the treatment of RA and its cardiac complications in the clinic.

Background Identification and analysis of influencing factors of occupational injury is an important research content of feature selection. In recent years, with the rise of machine learning algorithms, feature selection combined with Boosting algorithm provides a new analysis idea to construct occupational injury prediction models. Objective To evaluate applicability of Boosting algorithm-based model in predicting severity of miners' non-fatal occupational injuries, and provide a basis for rationally predicting the severity level of miners' non-fatal occupational injuries. Methods The publicly available data of the US Mine Safety and Health Administration (MSHA) from 2001 to 2021 on metal miners' non-fatal occupational injuries were used, and the outcome variables were lost working days < 105 d (minor injury) and ≥ 105 d (serious injury). Four different feature sets were screened out by four feature selection methods including least absolute shrinkage and selection operator (Lasso) regression, stepwise regression, single factor + Lasso regression, and single factor + stepwise regression. Logistic regression, gradient boosting decision tree (GBDT), and extreme gradient boosting (XGBoost) were selected to construct prediction models by training with the four feature sets. A total of 12 prediction models of severity of miners' non-fatal occupational injuries were built and their area under the curve (AUC), sensitivity, specificity, and Youden index were calculated for model evaluation. Results According to the results of four feature selection methods, age, time of accident occurrence, total length of service, cause of injury, activities that triggered injury occurrence, body part of injury, nature of injury, and outcome of injury were identified as influencing factors of non-fatal occupational injury severity in miners. Feature set 4 was the optimal set screened out by single factor+stepwise regression and the GBDT model presented the best predictive performance in predicting the severity of non-fatal occupational injuries. The associated specificity, sensitivity, and Youden index were 0.7530, 0.9490, and 0.7020, respectively. The AUC values of logistic regression, GBDT, and XGBoost models trained by feature set 4 were 0.8526 (95%CI: 0.8387, 0.8750), 0.8640 (95%CI: 0.8474, 0.8806), and 0.8603 (95%CI: 0.8439, 0.8773), respectively, higher than the AUC values trained by feature set 2 [0.8487 (95%CI: 0.8203, 0.8669), 0.8110 (95%CI: 0.8012, 0.8344), and 0.8439 (95%CI: 0.8245, 0.8561), respectively] . The AUC values of GBDT and XGBoost models trained by feature set 4 were higher than that of logistic regression model. Conclusion The performance of the prediction models constructed by predictors screened out by two feature selection methods is better than those by single feature selection methods. At the same time, under the condition of optimal feature set, the performance of model prediction based on Boosting is better than that of traditional logistic regression model.

Background Occupational injuries are one of the leading causes of death or disability in occupational populations. According to the World Health Organization and the International Labour Organization, occupational injuries were the occupational contributor responsible for the largest loss of disability-adjusted life years (DALY) globally in 2016. Objective To analyze the burden of deaths attributed to occupational injuries in Chinese population from 1990 to 2019, and provide a reference for further construction of occupational injury surveillance system. Methods Using the results and data of the Global Burden of Disease 2019 (GBD 2019), this study estimated the burden of deaths attributable to occupational injuries by year, sex, and age groups, and the indicators included deaths, years of life lost (YLL), mortality, and YLL rates. Age-standardized rates of deaths and YLL rates were calculated using a world standard population presented by GBD 2019. Annualized rate of change (ARC) was use to evaluate changes in the indicators over time. All results were presented as point estimates with 95% uncertainty intervals (95%UI). Results In 2019, the deaths attributable to occupational injuries among women in China accounted for 33.16% of that among world's women, their YLL accounted for 31.88%, and the two indicators among Chinese men accounted for 17.98% and 17.09%, respectively. Compared with 1990, the standardized mortality rate and the standardized YLL rate attributable to occupational injuries in China in 2019 decreased, among which the ARCs of the standardized mortality rate in the whole population, men, and women were −0.68 (95%UI: −0.78, −0.51), −0.68 (95%UI: −0.80, −0.47), and −0.68 (95%UI: −0.82, −0.46), respectively. The ARCs of the standardized YLL rate in the whole population, men, and women were −0.68 (95%UI: −0.78, −0.51), −0.67 (95%UI: −0.80, −0.48), and −0.68 (95%UI: −0.81, −0.44), respectively. Absolute values of the ARCs of the standardized mortality rate and the standardized YLL rate attributable to occupational injuries from 1990 to 2010 were higher than those from 2010 to 2019. The ARCs of the standardized YLL rate for road injuries, falls, and drowning from 1990 to 2010 were −0.55 (95%UI: −0.67, −0.36), −0.57 (95%UI: −0.73, −0.38), −0.77 (95%UI: −0.84, −0.63), and the ARCs from 2010 to 2019 were −0.27 (95%UI: −0.46, −0.02), −0.07 (95%UI: −0.34, −0.26), −0.06 (95%UI: −0.32, −0.29), respectively. In 2019, the standardized mortality rate attributable to occupational injuries among Chinese men was 5.68/100000 (95%UI: 3.89/100000, 8.23/100000), and the standardized YLL rate was 286.27/100000 (95%UI: 197.58/100000, 411.38/100000); the standardized mortality rate attributable to occupational injuries among Chinese women was 1.55/100000 (95%UI: 0.99/100000, 2.36/100000), and the standardized YLL rate was 80.85/100000 (95%UI: 51.61/100000, 122.07/100000). Conclusion From 1990 to 2019, the burden of deaths attributable to occupational injuries in China is declined, but the rate of decline is slowed down in the last decade. The burden of deaths attributable to occupational injuries among women in China still accounts for a high proportion of the global burden among women. The burden of deaths attributable to occupational injuries among Chinese men is higher than that among Chinese women.

Background Occupational injury is one of the important causes of death among the working population and a worldwide hot topic, but there are few relevant studies on the trend and prediction of occupational injury attributable deaths in China. Objective To analyze the trend of occupational injury attributable deaths in China from 2000 to 2019, predict the deaths of occupational injuries in China from 2020 to 2024 by contructing a gray GM(1,1) model, and provid a reference for surveillance and assessment of occupational injuries. Methods Mortality, crude mortality rates, and standardized mortality rates of occupational injuries in China by year, sex, and age groups were calculated using data of the Global Burden of Disease (GBD) 2019 study. Join-point model was used to analyze possible trend of standardized mortality rate from 2000 to 2019, and calculate annual percentage change (APC) and average annual percentage change (AAPC). After a gray model GM(1,1) was established, the accuracy of the model was evaluated by posterior error ratio (C) and small error probability (P) and rated as Level 1 (good, C≤0.35 and P≥0.95) or Level 2 (qualified, 0.35<C≤0.50 and 0.80≤P<0.95). Then the gray model was further used to predict the number of deaths and standardized mortality rates of occupational injuries in China from 2020 to 2024. Results From 2000 to 2019, the deaths due to occupational injuries in China showed a downward trend, the number of deaths decreased from 111557 to 61780, the crude mortality rate decreased from 8.58/100000 to 4.34/100000, the standardized mortality rate decreased from 7.67/100000 to 3.65/100000, and the AAPC of standardized mortality rate was −4.0% (P<0.05); the number of male deaths decreased from 87760 to 49192, and the male standardized mortality rate decreased from 11.78/100000 to 5.68/100000; the number of female deaths decreased from 23797 to 12588, and the female standardized mortality rate decreased from 3.34/100000 to 1.55/100000; the AAPCs of male and female standardized mortality rate were −3.9% and −4.1% respectively. The accuracy of the established gray model for deaths (C=0.09, P=1) was rated as Level 1, and that for standardized mortality rate (C=0.41, P=0.9) was rated as level 2, which allowed for prediction extrapolation. The model showed that from 2020 to 2024, the number of occupational injury attributable deaths would be 76039, 73849, 71721, 69655, and 67649, and the standardized mortality rate would be 4.23/100000, 4.07/100000, 3.92/100000, 3.77/100000, and 3.62/100000, respectively. Conclusion From 2000 to 2019, the standardized mortality rate of occupational injuries in China showed a downward trend, and it is predicted that the standardized mortality rate from 2020 to 2024 will still show a downward trend, but the number of deaths will remain high, so it is necessary to continue to strengthen prevention and control of occupational injuries.