Objective: This study aimed to evaluate the prognostic value of [ 18F]ALF-NOTA-FAPI-04 PET/CT-derived parameters, including the maximum standardized uptake value (SUVmax), FAPI-avid tumor volume (FTV), and total lesion FAP expression (TLF), in patients with recurrent gastric cancer undergoing combined chemoimmunotherapy. We sought to establish a noninvasive imaging biomarker framework to optimize patient stratification and therapeutic decision-making. Materials and Methods: This retrospective cohort study analyzed 51 patients with recurrent gastric cancer who received programmed cell death protein 1 (PD-1) inhibitors combined with chemotherapy after gastrectomy. All patients underwent [ 18F]ALF-NOTA-FAPI-04 PET/CT within 14 days of chemoimmunotherapy. Semi-quantitative parameters (SUVmax, FTV, and TLF) were derived using semi-automated tumor segmentation. The primary endpoint of this study was the assessment of the clinical efficacy of chemoimmunotherapy, categorized as a durable or nondurable clinical benefit. The secondary endpoints included progression-free survival (PFS) and overall survival (OS). In a subgroup of 16 patients who underwent sequential [ 18F]FDG PET/CT within seven days of [ 18F]ALF-NOTA-FAPI-04 PET/CT, tracer uptake values were compared between the two PET/CT examinations. Results: 51 patients were included. The median PFS and OS were 7 and 10 months, respectively. Durable clinical benefit (DCB) was observed in 30 patients and showed significantly lower SUVmax, FTV, and TLF values than non-DCB. TLF demonstrated the highest diagnostic accuracy for DCB (area under the receiver operating characteristic curve [AUC] = 0.80). Multivariable analysis identified TLF ≥ 188.88 SUVbw·cm 3 as an independent factor associated with PFS (hazard ratio [HR] = 7.29, P = 0.001) and FTV ≥ 44.17 cm 3 as an independent factor associated with OS (HR = 5.16, P = 0.010). In the subgroup analysis of 16 patients, semi-quantitative analysis demonstrated consistently higher values of [ 18F]ALF-NOTA-FAPI-04-derived parameters than [ 18F]FDG-derived parameters (all P < 0.001). Conclusion [ 18F]ALF-NOTA-FAPI-04 PET/CT may be a useful imaging tool for predicting clinical outcomes in patients with recurrent gastric cancer undergoing treatment with PD-1 inhibitors and chemotherapy.

Objective To provide a comprehensive review of the modeling method of the empty bottle stimulation(EBS)anxiety model,including commonly used experimental animal strains and genders,animal grouping,modeling procedures,modeling duration,primary behavioral evaluation method,and the underlying pathological mechanisms.This aims to offer a reference for the application of the EBS anxiety model in anxiety disorder research.Methods Searches were conducted in databases such as CNKI and PubMed to collect all literature related to the EBS anxiety model,which were then systematically summarized and organized.Results(1)Male adult SD or Wistar rats are predominantly used as experimental animals;(2)The optimal modeling period is 2 weeks;(3)Behavioral evaluations primarily utilize the open field test,elevated plus maze test,and light-dark box test;(4)Pathological mechanisms involve abnormal neurotransmitter metabolism in brain regions such as the hippocampus,prefrontal cortex,and amygdala.Conclusions The EBS anxiety model exhibits an anxiety-like behavioral phenotype and associated neurobiological mechanisms,validating its utility as an animal model for the study of anxiety disorders.However,further exploration and refinement are required for its standardized construction protocol and the understanding of its mechanistic underpinnings.

Objective:To explore the relationship between stress perception and problematic short video use among college students, as well as the chain mediating role of mind wandering and self-control.Methods:From October to November 2023, a cross-sectional survey of 434 college students was conducted by the stress perception scale, the problematic short video media use scale, the mind wandering scale and the self-control scale. Common method bias test, descriptive statistics and Pearson correlation analysis were performed using SPSS 27.0 statistical software, and Bootstrap method of PROCESS 4.0 macro program was used for chain mediation effect test.Results:The scores of stress perception, mind wandering, self-control and problematic short video use were (38.03±7.28), (16.08±4.71), (64.27±10.34), and (36.80±8.70).Pearson correlation analysis showed that stress perception, mind wandering and problematic short video use were significantly positively correlated ( r=0.35, 0.43, 0.57, all P<0.01). Stress perception, mind wandering and problematic short video use were significantly negatively correlated with self-control ( r=-0.55, -0.59, -0.54, all P<0.01). The mediating effect results showed that direct effect was not significant ( P>0.05), and mind wandering and self-control played complete mediating effects.Stress perception affected the problematic short video use through the following three pathways: the mediating effect size of mind wandering was 0.202 (95% CI=0.134-0.276). The effect size of self-control was 0.133 (95% CI=0.078-0.198). The chain mediating effect size of mind wandering and self-control was 0.069 (95% CI=0.040-0.104). Conclusion:Stress perception can indirectly affect the problematic short video use through the complete chain mediation of mind wandering and self-control.

Objective To provide a comprehensive review of the modeling method of the empty bottle stimulation(EBS)anxiety model,including commonly used experimental animal strains and genders,animal grouping,modeling procedures,modeling duration,primary behavioral evaluation method,and the underlying pathological mechanisms.This aims to offer a reference for the application of the EBS anxiety model in anxiety disorder research.Methods Searches were conducted in databases such as CNKI and PubMed to collect all literature related to the EBS anxiety model,which were then systematically summarized and organized.Results(1)Male adult SD or Wistar rats are predominantly used as experimental animals;(2)The optimal modeling period is 2 weeks;(3)Behavioral evaluations primarily utilize the open field test,elevated plus maze test,and light-dark box test;(4)Pathological mechanisms involve abnormal neurotransmitter metabolism in brain regions such as the hippocampus,prefrontal cortex,and amygdala.Conclusions The EBS anxiety model exhibits an anxiety-like behavioral phenotype and associated neurobiological mechanisms,validating its utility as an animal model for the study of anxiety disorders.However,further exploration and refinement are required for its standardized construction protocol and the understanding of its mechanistic underpinnings.

Objective:To explore the relationship between stress perception and problematic short video use among college students, as well as the chain mediating role of mind wandering and self-control.Methods:From October to November 2023, a cross-sectional survey of 434 college students was conducted by the stress perception scale, the problematic short video media use scale, the mind wandering scale and the self-control scale. Common method bias test, descriptive statistics and Pearson correlation analysis were performed using SPSS 27.0 statistical software, and Bootstrap method of PROCESS 4.0 macro program was used for chain mediation effect test.Results:The scores of stress perception, mind wandering, self-control and problematic short video use were (38.03±7.28), (16.08±4.71), (64.27±10.34), and (36.80±8.70).Pearson correlation analysis showed that stress perception, mind wandering and problematic short video use were significantly positively correlated ( r=0.35, 0.43, 0.57, all P<0.01). Stress perception, mind wandering and problematic short video use were significantly negatively correlated with self-control ( r=-0.55, -0.59, -0.54, all P<0.01). The mediating effect results showed that direct effect was not significant ( P>0.05), and mind wandering and self-control played complete mediating effects.Stress perception affected the problematic short video use through the following three pathways: the mediating effect size of mind wandering was 0.202 (95% CI=0.134-0.276). The effect size of self-control was 0.133 (95% CI=0.078-0.198). The chain mediating effect size of mind wandering and self-control was 0.069 (95% CI=0.040-0.104). Conclusion:Stress perception can indirectly affect the problematic short video use through the complete chain mediation of mind wandering and self-control.

Objective: To translate the Menopause Perception Scale (MPS) into Chinese, and evaluate the reliability and validity of the Chinese version of the MPS. Methods: The MPS was translated back-translated, culturally adapted and pre-tested according to the Brislin translation model to develop the Chinese version of MPS. The menopausal women from five communities were selected using simple random sampling to assess the reliability and validity of the Chinese version of MPS. Content validity was evaluated based on expert ratings, criterion-related validity was evaluated using the Chinese version of the Menopause Rating Scale (MRS) as the criterion. Structural validity was evaluated using exploratory factor analysis and confirmatory factor analysis. Internal consistency was evaluated using Cronbach's α, and split-half reliability and test-retest reliability were calculated. Results: Totally 430 questionnaires were allocated, and 414 valid questionnaires were recovered, with an effective recovery rate of 96.28%. The Chinese version of the MPS consisted of 18 items across four dimensions: acceptance, sexual perception, normalization, and support perception. The item-level content validity index ranged from 0.833 to 1.000, and the scale-level content validity index average was 0.924. The correlation coefficients between the scores of each dimension and the total scores of the Chinese version of the MPS and the Chinese version of the MRS ranged from 0.529 to 0.790 (all P<0.05). Exploratory factor analysis extracted four common factors, with a cumulative variance contribution rate of 64.502%. Confirmatory factor analysis showed good model fit, with a root mean square error of approximation of 0.052, root mean square residual of 0.053, comparative fit index of 0.958, Tucker-Lewis index of 0.950, goodness of fit index of 0.908, incremental fit index of 0.958, and relative fit index of 0.884. The Cronbach's α of the Chinese version of the MPS was 0.916, the split-half reliability coefficient was 0.845, and the test-retest reliability was 0.906. Conclusion The Chinese version of the MPS demonstrates good reliability and validity, and can be used to assess the perceptions and attitudes of menopausal women in China toward menopause.

BACKGROUND: Arsenic trioxide (ATO) is indicated as a broad-spectrum medicine for a variety of diseases, including cancer and cardiac disease. While the role of ATO in hepatic ischemia/reperfusion injury (HIRI) has not been reported. Thus, the purpose of this study was to identify the effects of ATO on HIRI. METHODS: In the present study, we established a 70% hepatic warm I/R injury and partial hepatectomy (30% resection) animal models in vivo and hepatocytes anoxia/reoxygenation (A/R) models in vitro with ATO pretreatment and further assessed liver function by histopathologic changes, enzyme-linked immunosorbent assay, cell counting kit-8, and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay. Small interfering RNA (siRNA) for extracellular signal-regulated kinase (ERK) 1/2 was transfected to evaluate the role of ERK1/2 pathway during HIRI, followed by ATO pretreatment. The dynamic process of autophagic flux and numbers of autophagosomes were detected by green fluorescent protein-monomeric red fluorescent protein-LC3 (GFP-mRFP-LC3) staining and transmission electron microscopy. RESULTS: A low dose of ATO (0.75 μmol/L in vitro and 1 mg/kg in vivo ) significantly reduced tissue necrosis, inflammatory infiltration, and hepatocyte apoptosis during the process of hepatic I/R. Meanwhile, ATO obviously promoted the ability of cell proliferation and liver regeneration. Mechanistically, in vitro  studies have shown that nontoxic concentrations of ATO can activate both ERK and phosphoinositide 3-kinase-serine/threonine kinase (PI3K-AKT) pathways and further induce autophagy. The hepatoprotective mechanism of ATO, at least in part, relies on the effects of ATO on the activation of autophagy, which is ERK-dependent. CONCLUSION Low, non-toxic doses of ATO can activate ERK/PI3K-AKT pathways and induce ERK-dependent autophagy in hepatocytes, protecting liver against I/R injury and accelerating hepatocyte regeneration after partial hepatectomy.
Animals ; Arsenic Trioxide ; Autophagy/physiology* ; Reperfusion Injury/prevention & control* ; Mice ; Male ; Proto-Oncogene Proteins c-akt/physiology* ; Arsenicals/therapeutic use* ; Oxides/therapeutic use* ; Liver/metabolism* ; Extracellular Signal-Regulated MAP Kinases/metabolism* ; Mice, Inbred C57BL

Objective This study aimed to investigate the effects of sleep interruption(SI)cycles on emotional behavior in ICR mice,and to establish a mouse model of comorbid anxiety and depression induced by SI.Methods Seventy-two male ICR mice(4～5 weeks old)were divided randomly into a blank group and a model group.Mice in the model group were subjected to SI stress modeling for 1,2,and 3 weeks,respectively.After modeling,emotional behaviors were evaluated using open-field,elevated plus maze,light-dark box,marble-burying,and forced-swimming tests.Serum corticosterone levels were detected by enzyme-linked immunosorbent assay.Results Mice in the model group buried significantly more marbles after 1 week of SI stress,compared with the blank group(P＜0.05).After 2 weeks of stress,mice in the model group also showed a significant decrease in the number of crossings in the light-dark box(P＜0.05)and a significant increase in the number of marbles buried(P＜0.01)compared with the control group.After 3 weeks of stress,mice in the model group showed a significant increase in the number of marbles buried(P＜0.05),a significant decrease in the number of crossings in the light-dark box(P＜0.05),and a significant increase in immobility time in the forced-swim test(P＜0.01).Conclusions ICR mice exhibited significant anxiety-related behaviors after 2 weeks of SI modeling and significant anxiety-and depressive-related behavioral changes after 3 weeks.Three weeks of SI stress can be used to establish a model of comorbid anxiety and depression.

Objective This study aimed to investigate the effects of sleep interruption(SI)cycles on emotional behavior in ICR mice,and to establish a mouse model of comorbid anxiety and depression induced by SI.Methods Seventy-two male ICR mice(4～5 weeks old)were divided randomly into a blank group and a model group.Mice in the model group were subjected to SI stress modeling for 1,2,and 3 weeks,respectively.After modeling,emotional behaviors were evaluated using open-field,elevated plus maze,light-dark box,marble-burying,and forced-swimming tests.Serum corticosterone levels were detected by enzyme-linked immunosorbent assay.Results Mice in the model group buried significantly more marbles after 1 week of SI stress,compared with the blank group(P＜0.05).After 2 weeks of stress,mice in the model group also showed a significant decrease in the number of crossings in the light-dark box(P＜0.05)and a significant increase in the number of marbles buried(P＜0.01)compared with the control group.After 3 weeks of stress,mice in the model group showed a significant increase in the number of marbles buried(P＜0.05),a significant decrease in the number of crossings in the light-dark box(P＜0.05),and a significant increase in immobility time in the forced-swim test(P＜0.01).Conclusions ICR mice exhibited significant anxiety-related behaviors after 2 weeks of SI modeling and significant anxiety-and depressive-related behavioral changes after 3 weeks.Three weeks of SI stress can be used to establish a model of comorbid anxiety and depression.

Objective To analyze the phonetic features of patients with chronic obstructive pulmonary disease(COPD)by using modern acoustic diagnosis technology,in order to provide an objective basis for the informationized diagnosis of COPD patients in traditional Chinese medicine.Methods The voice signals of COPD group and control group were collected,and the characteristic parameters were extracted by modern speech analysis method to analyze the sound diagnosis characteristics of COPD patients.Results There were significant differences between COPD group and control group(P＜0.05).There were significant differences in the phonetic features of different COPD severity groups(P＜0.05),and showed a certain trend with the change of severity.There were significant differences among different COPD groups(P＜0.05).In pairwise comparison,there were significant differences among the syndrome types(P＜0.05),and they had certain characteristics.Conclusion There are certain rules in the phonetic features of COPD and its different syndromes,which can provide objective reference for the diagnosis of COPD and TCM syndrome differentiation.