Depression, a mental health disorder, has emerged as one of the significant challenges in the global public health domain. Investigating the pathogenesis of depression and accurately assessing the symptomatic changes are fundamental to formulating effective clinical diagnosis and treatment strategies. Utilizing non-invasive brain imaging technologies such as functional magnetic resonance imaging and scalp electroencephalography, existing studies have confirmed that the onset of depression is closely associated with abnormal neural activities and altered functional connectivity in multiple brain regions. Magnetoencephalography, unaffected by tissue conductivity and skull thickness, boasts high spatial resolution and signal-to-noise ratio, offering unique advantages and significant value in revealing the abnormal brain mechanisms and neural characteristics of depression. This review, starting from the rhythmic characteristics, nonlinear dynamic features, and connectivity characteristics of magnetoencephalography in depression patients, revisits the research progress on magnetoencephalography features related to depression, discusses current issues and future development trends, and provides insights for the study of pathophysiological mechanisms, as well as for clinical diagnosis and treatment of depression.
Humans ; Magnetoencephalography/methods* ; Brain/physiopathology* ; Depression/diagnosis* ; Electroencephalography ; Magnetic Resonance Imaging

Objective To investigate the relationship between different body position fixation tech-niques,umbilical plane volume change(ΔV),body weight change rate(ΔW%),and radiotherapy setup errors in cervical cancer patients,and to provide recommendations for determining the margin of planning target vol-ume(MPTV).Methods A retrospective analysis was performed on the clinical data of 57 cervical cancer pa-tients who underwent radiotherapy at this hospital from June 2022 to May 2023.Patients were divided into the observation group(fixed with body membrane+thermoplastic pad,n=24)and the control group(fixed with body membrane alone,n=33)based on different positioning fixation methods.They were also further strati-fied by median BMI into BMI≥23.82 kg/m2 and BMI<23.82 kg/m2 patients.Setup errors in the left-right(X),cranio-caudal(Y),and anterior-posterior(Z)directions were recorded.Meanwhile,the umbilical plane volume on the first CT positioning image and the patient's body weight before positioning were recorded,as well as umbilical plane volume of cone-beam CT(CBCT)verification images during weekly radiotherapy and body weight before scan,the ΔV and ΔW%were calculated.Setup errors were compared between two groups,and correlations between ΔV,ΔW%and setup errors were analyzed in all patients,in two groups(the obser-vation group and the control group)and in two BMI subgroups.MPTV values in X,Y,and Z directions were calculated,and receiver operating characteristic(ROC)curve determined the cut off values of ΔV and ΔW%when setup errors met the department's MPTV criteria.Results Compared with the control group,the ob-servation group showed significantly smaller setup errors in X,Y,and Z directions(P<0.05).Both ΔV and ΔW%were positively correlated with setup errors in X and Y direction in all patients,patients in the control group,and patients with BMI≥23.82 kg/m2(P<0.05).In the observation group,ΔW%was positively corre-lated with setup errors in Z direction in patients with BMI<23.82 kg/m2(P<0.05);In the control group,ΔV and ΔW%were positively correlated with setup errors in X and Y direction in patients with BMI≥23.82 kg/m2.ROC curve analysis showed that when setup errors in Y direction reached the department's MPTV criteria(8.41 mm),the cutoff values ΔV and ΔW%were 8.045 cm2 and 4.12%,respectively.Conclusion The body membrane+thermoplastic pad fixation technique reduces setup errors and mitigates the impact of ΔV and ΔW%on setup errors in X and Y directions.When ΔV or ΔW%exceeds the cutoff values,increasing CBCT verification frequency and re-fabricating the body membrane are recommended.

Objective:To explore the relationship between stress perception and problematic short video use among college students, as well as the chain mediating role of mind wandering and self-control.Methods:From October to November 2023, a cross-sectional survey of 434 college students was conducted by the stress perception scale, the problematic short video media use scale, the mind wandering scale and the self-control scale. Common method bias test, descriptive statistics and Pearson correlation analysis were performed using SPSS 27.0 statistical software, and Bootstrap method of PROCESS 4.0 macro program was used for chain mediation effect test.Results:The scores of stress perception, mind wandering, self-control and problematic short video use were (38.03±7.28), (16.08±4.71), (64.27±10.34), and (36.80±8.70).Pearson correlation analysis showed that stress perception, mind wandering and problematic short video use were significantly positively correlated ( r=0.35, 0.43, 0.57, all P<0.01). Stress perception, mind wandering and problematic short video use were significantly negatively correlated with self-control ( r=-0.55, -0.59, -0.54, all P<0.01). The mediating effect results showed that direct effect was not significant ( P>0.05), and mind wandering and self-control played complete mediating effects.Stress perception affected the problematic short video use through the following three pathways: the mediating effect size of mind wandering was 0.202 (95% CI=0.134-0.276). The effect size of self-control was 0.133 (95% CI=0.078-0.198). The chain mediating effect size of mind wandering and self-control was 0.069 (95% CI=0.040-0.104). Conclusion:Stress perception can indirectly affect the problematic short video use through the complete chain mediation of mind wandering and self-control.

Objective:To explore the relationship between stress perception and problematic short video use among college students, as well as the chain mediating role of mind wandering and self-control.Methods:From October to November 2023, a cross-sectional survey of 434 college students was conducted by the stress perception scale, the problematic short video media use scale, the mind wandering scale and the self-control scale. Common method bias test, descriptive statistics and Pearson correlation analysis were performed using SPSS 27.0 statistical software, and Bootstrap method of PROCESS 4.0 macro program was used for chain mediation effect test.Results:The scores of stress perception, mind wandering, self-control and problematic short video use were (38.03±7.28), (16.08±4.71), (64.27±10.34), and (36.80±8.70).Pearson correlation analysis showed that stress perception, mind wandering and problematic short video use were significantly positively correlated ( r=0.35, 0.43, 0.57, all P<0.01). Stress perception, mind wandering and problematic short video use were significantly negatively correlated with self-control ( r=-0.55, -0.59, -0.54, all P<0.01). The mediating effect results showed that direct effect was not significant ( P>0.05), and mind wandering and self-control played complete mediating effects.Stress perception affected the problematic short video use through the following three pathways: the mediating effect size of mind wandering was 0.202 (95% CI=0.134-0.276). The effect size of self-control was 0.133 (95% CI=0.078-0.198). The chain mediating effect size of mind wandering and self-control was 0.069 (95% CI=0.040-0.104). Conclusion:Stress perception can indirectly affect the problematic short video use through the complete chain mediation of mind wandering and self-control.

Objective:To investigate the effect of DNA methylation of laminin α3 (LAMA3) on the prognosis of platinum-resistant epithelial ovarian cancer (EOC) and its possible mechanism.Methods:(1) The relationship between DNA methylation of LAMA3 and platinum resistance in EOC was evaluated by bioinformatics. (2) A total of 67 EOC patients treated at Guangxi Medical University Cancer Hospital from January 2000 to December 2012 were selected to detect the levels of LAMA3 DNA methylation in EOC tissues using pyrophosphate sequencing technology to explore its diagnostic efficacy for platinum resistance and prognosis in EOC patients. Furthermore, its impact on chemotherapy efficacy and prognosis of platinum resistant EOC patients were also analyzed.Results:(1) Ten proteins highly interacting with LAMA3 were screened from the Gene Interaction Retrieval Platform (STRING) database, including laminin β (LAMB) 3, laminin γ (LAMC) 3, integrin α (ITGA) 6, intestine protein β4 (ITGB4), ITGA3, LAMC1,LAMB2, dystrophin associated glycoprotein 1 (DAG1), LAMB1 and cytochrome P450c17α (COL17A1) protein; kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis showed that LAMA3 and its related interacting proteins participate in the regulation of malignant tumor occurrence and development through signaling pathways such as apoptosis, cell cycle, DNA damage response, epithelial mesenchymal transition (EMT), androgen receptor (AR), estrogen receptor (ER), phosphatidylinositol 3 kinase (PI3K)/protein kinase B (Akt), RAS/mitogen activated protein kinase (MAPK), receptor tyrosine kinase (RTK), tuberous sclerosis protein complex (TSC)/mammalian target of rapamycin (mTOR), and their expression levels were related to the sensitivity of chemotherapy drugs such as cisplatin in EOC. (2) Our clinical data analysis found that the LAMA3 DNA methylation level in EOC tissue of the platinum-sensitive group (35 cases) was 71% (25/35), which was higher than 69% (22/32) in the platinum-resistant group (32 cases), with statistically insignificant difference ( χ2=0.057, P=0.811). The area under the curve (AUC) of LAMA3 DNA methylation level for assessing platinum resistance in EOC was 0.601, and the AUC for predicting EOC patient prognosis was 0.686. The chemotherapy efficacy of EOC patients with high methylation of LAMA3 DNA was worse than that of patients with low methylation, 50% (12/24) vs 15/15, with statistically significant difference ( χ2=10.833, P=0.001). The level of LAMA3 DNA methylation had a significant impact on the progression free survival and overall survival of EOC patients (both P<0.05). Conclusion:The level of LAMA3 DNA methylation has certain diagnostic and predictive value for platinum resistance and prognosis in EOC patients, which may be closely related to the regulatory mechanism, platinum resistance and prognosis of EOC.

Objective:To comprehensively evaluate the tricuspid valve, right heart anatomical characteristics and related dynamic parameters in patients with different degrees of functional tricuspid regurgitation (FTR) using four-dimensional auto tricuspid valve quantitative(4D Auto TVQ), four-dimensional auto right ventricle quantitative(4D Auto RVQ), and four-dimensional auto left atrium quantitative(4D Auto LAQ), and to investigate the structural and functional changes of the tricuspid valve and right heart in them.Methods:Sixty-three patients with FTR diagnosed by echocardiography at the First Affiliated Hospital of Guangxi Medical University from February to July 2022 were prospectively selected as the case group, including 30 patients with mild FTR and 33 patients with moderate or above FTR, and 30 healthy subjects were selected as the control group. Transthoracic echocardiography was used for two-dimensional and three-dimensional image acquisition of the heart. The tricuspid regurgitation volume, left ventricular ejection fraction (LVEF), right ventricular global strain (RVGS) were measured by 2D images, and pulmonary artery systolic pressure (PASP) were measured from the tricuspid regurgitation pressure difference. The 3D images were imported into EchoPAC 204 to obtain the tricuspid valve, right heart structure and related dynamic parameters. The annulus area (AA), annulus perimeter(AP), spherical index (SI), annulus area change fraction (AC), coaptation point height (CPH), and tenting volume (TV) were measured by 4D Auto TVQ. The right atrial maximum volume (RAVmax) and right atrial minimum volume (RAVmin) were measured by 4D Auto LAQ. Right ventricular end-diastolic volume (RVEDV), right ventricular end-systolic volume (RVESV), right ventricular fractional area change (RVFAC) and tricuspid annular plane systolic excursion (TAPSE) were measured by 4D Auto RVQ. After standardizing the dimension parameters with body surface area (BSA), the differences in the above parameters were compared between the three groups, the correlation between regurgitant volume and each parameter was compared by correlation analysis, and the independent factors of increased tricuspid regurgitant volume were investigated by univariate and multivariate linear regression analysis.Results:There were statistically significant differences in PASP, AA/BSA, AP/BSA, AC, TV, RAVmax/BSA, RAVmin/BSA, RVFAC, RVGS, and TAPSE between the three groups (all P<0.05). There were statistically significant differences in LVEF, CPH, RVEDV/BSA, and RVESV/BSA in the moderate and above FTR group compared with the control and mild FTR groups (all P<0.05). Correlation analysis showed that RAVmin was the most highly correlated with tricuspid regurgitant volume ( r=0.875, P<0.001) and TV and end-systolic annulus area(ESAA) were highly correlated with tricuspid regurgitant volume ( r=0.747, 0.683; both P<0.001) in patients with FTR. Multifactorial linear regression showed that RAVmin, TV and regurgitant volume were independently positively correlated (β=0.721, 0.205; both P<0.05). Conclusions:The four quantification technique can provide valid structural and functional information by quantifying the tricuspid valve as well as the right heart in patients with FTR, and RAVmin and TV are independent correlates of increased tricuspid regurgitant volume.

Oral potentially malignant disorders (OPMDs) are precursors of oral squamous cell carcinoma (OSCC). Deregulated cellular energy metabolism is a critical hallmark of cancer cells. Peroxisome proliferator-activated receptor-gamma coactivator-1 alpha (PGC1α) plays vital role in mitochondrial energy metabolism. However, the molecular mechanism of PGC1α on OPMDs progression is less unclear. Therefore, we investigated the effects of knockdown PGC1α on human dysplastic oral keratinocytes (DOKs) comprehensively, including cell proliferation, cell cycle, apoptosis, xenograft tumor, mitochondrial DNA (mtDNA), mitochondrial electron transport chain complexes (ETC), reactive oxygen species (ROS), oxygen consumption rate (OCR), extracellular acidification rate (ECAR), and glucose uptake. We found that knockdown PGC1α significantly inhibited the proliferation of DOKs in vitro and tumor growth in vivo, induced S-phase arrest, and suppressed PI3K/Akt signaling pathway without affecting cell apoptosis. Mechanistically, downregulated of PGC1α decreased mtDNA, ETC, and OCR, while enhancing ROS, glucose uptake, ECAR, and glycolysis by regulating lactate dehydrogenase A (LDHA). Moreover, SR18292 (an inhibitor of PGC1α) induced oxidative phosphorylation dysfunction of DOKs and declined DOK xenograft tumor progression. Thus, our work suggests that PGC1α plays a crucial role in cell proliferation by reprograming energy metabolism and interfering with energy metabolism, acting as a potential therapeutic target for OPMDs.
Humans ; Carcinoma, Squamous Cell/metabolism* ; Cell Proliferation ; DNA, Mitochondrial ; Energy Metabolism ; Glucose ; Mouth Neoplasms/metabolism* ; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha/metabolism* ; Phosphatidylinositol 3-Kinases ; Reactive Oxygen Species

Objective To establish a UHPLC method for the determination of zolpidem tartrate tablets after radiation, and to investigate the effect of different radiation doses on the content of zolpidem tartrate tablets. Methods Ultra high performance liquid chromatography was used. The content of zolpidem tartrate tablets irradiated by γ-ray was determined. Using C₁₈ column, acetonitrile methanol-0.05 mol/L phosphoric acid solution (the pH value as 5.5 with triethylamine) (18∶26∶56) was used as the mobile phase. The flow rate was 0.7 ml/min, and the detection wavelength was 254 nm. Results The method validation showed good linearity in the concentration range of 5-80 μg/ml (r=0.999 6); The average recovery was 98.2%, RSD was 1.72%, and the repeatability was 0.87%. The contents of zolpidem tartrate were 105.1%, 106.4%, 102.7% and 105.4% under 0, 8, 25 and 80 kGy radiation. Conclusion UHPLC has accurate results with short analysis cycle in this study. It is suitable for the determination of zolpidem tartrate tablets after radiation. The content of zolpidem tartrate tablets remained basically unchanged after radiation.

Objective:To investigate the risk factors of acute kidney injury (AKI) in patients with severe drug eruption.Methods:Clinical data including basic information (gender, age, body mass index), type of severe drug eruption, allergenic drugs, co-existing diseases, first laboratory findings after admission, renal function during hospitalization, etc. in patients with severe drug eruption admitted to Department of Dermatology, Guangdong Provincial People′s Hospital from January 2014 to December 2020 were collected and analyzed retrospectively. Patients were divided into AKI group and non-AKI group and the clinical data were compared between the 2 groups. The risk factors of AKI in patients with severe drug eruption were analyzed by a binary logistic regression model and the odds ratio ( OR) and its 95% confidence interval ( CI) were calculated. Results:A total of 91 patients were enrolled in the study, including 50 males and 41 females, aged 54 (40, 65) years with a range of 9-85 years. The drugs that induced severe drug eruptions were allopurinol in 48 patients, antiepileptic drugs in 23 patients, antibacterial drugs in 6 patients, and other drugs in 14 patients. There were 21 patients (AKI incidence: 23.1%) in the AKI group and 70 patients in the non-AKI group. The proportion of patients sensitized to antiepileptic drugs, baseline estimated glomerular filtration rate, levels of serum albumin and hemoglobin in the AKI group were lower than those in the non-AKI group [0 (0/21) vs. 32.9% (23/70), (70±12) ml/(min·1.73 m 2) vs. (103±6) ml/(min·1.73 m 2), 26(23, 30) g/L vs. 36(34, 38) g/L, (116±17) g/L vs. (129±15) g/L], the proportions of patients sensitized to allopurinol, with diabetes mellitus, and with chronic kidney diseases, baseline serum urea nitrogen level, serum creatinine level, and proportion of urinary albumin positive patients were all higher than those in the non-AKI group [81.0%(17/21) vs. 44.3%(31/70), 28.6%(6/21) vs. 7.1%(5/70), 28.6%(6/21) vs. 5.7%(4/70), 7.2 (4.6, 12.2) mmol/L vs. 5.0 (3.8, 6.4) mmol/L, 103 (63, 134) μmol/L vs. 67 (56, 79) μmol/L, 47.6%(10/21) vs. 17.1%(12/70)], and the differences were statistically significant (all P<0.05). Binary logistic regression analysis showed that allopurinol sensitization ( OR=6.588, 95 %CI: 1.006-43.123, P=0.049) was the independent risk factor for AKI in patients with severe drug eruption and serum albumin ( OR=0.595, 95 %CI: 0.471-0.752, P<0.001) was a protective factor. Conclusions:Allopurinol sensitization was the independent risk factor for AKI in patients with severe drug eruption. Patients with low serum albumin were more likely to develop AKI.