Objective:To examine the effects of preimplantation genetic testing for aneuploidies (PGT-A) using blastocyst biopsy on maternal and perinatal outcomes for women of advanced age.Methods:A retrospective cohort study was conducted during January 2016 to December 2018 at Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital. Women who were aged ≥35 years, underwent intracytoplasmic sperm injection (ICSI, control group) or PGT-A after ICSI (PGT-A group) with single frozen-thawed blastocyst transferred were eligible in this study. They were further divided into 35-37 years old subgroup and ≥38 years old subgroup according to age. The primary outcome was live birth, and the secondary outcomes were human chorionic gonadotropin (hCG) positivity, clinical pregnancy, pregnancy loss, hypertension in pregnancy, gestational diabetes mellitus, gestational age, preterm birth, caesarean section, low birth weight, small gestational age, and large gestational age.Results:For women aged ≥35 years, the live birth rate in PGT-A group was significantly higher than that in control group [38.0% (89/234) vs. 26.8% (237/885), OR(95% CI)=1.49(1.13-1.97), P=0.047], the miscarriage rate was significantly lower than that in control group [17.6% (19/108) vs. 29.0% (116/443), OR(95% CI)=0.45(0.24-0.85), P=0.013]. We found that for women who aged ≥38 years, the live birth rate [ OR(95% CI)=3.01(1.67-5.44), P<0.001], hCG positivity rate [ OR(95% CI)=2.08(1.25-3.47), P=0.005], clinical pregnancy rate [ OR(95% CI)=2.39(1.40-4.07), P=0.001] in PGT-A group were significantly higher than those in control group, and the miscarriage rate in PGT-A group was significantly lower than that in control group [ OR(95% CI)=0.34(0.13-0.85), P=0.022]; for women aged 35-37 years, there were no statistically significant differences in pregnancy outcomes between the two groups (all P>0.05). Moreover, there were no statistically significant differences in the rates of obstetric complications and perinatal outcomes for women aged ≥35 years between PGT-A group and control group (all P>0.05), and similar results were found in the subgroup analyses for women who aged 35-37 years or ≥38 years. Conclusion:PGT-A using blastocyst stage biopsy strategy in single blastocyst thaw transferred cycles could significantly improve the rates of hCG positivity, clinical pregnancy, and live birth, and significantly reduce the miscarriage rate for women aged ≥38 years, but could not improve the pregnancy outcomes for women aged 35-37 years. In addition, the use of PGT-A does not increase the risk of obstetric complications and perinatal outcomes for women of advanced age.

Objective:To examine the effects of preimplantation genetic testing for aneuploidies (PGT-A) using blastocyst biopsy on maternal and perinatal outcomes for women of advanced age.Methods:A retrospective cohort study was conducted during January 2016 to December 2018 at Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital. Women who were aged ≥35 years, underwent intracytoplasmic sperm injection (ICSI, control group) or PGT-A after ICSI (PGT-A group) with single frozen-thawed blastocyst transferred were eligible in this study. They were further divided into 35-37 years old subgroup and ≥38 years old subgroup according to age. The primary outcome was live birth, and the secondary outcomes were human chorionic gonadotropin (hCG) positivity, clinical pregnancy, pregnancy loss, hypertension in pregnancy, gestational diabetes mellitus, gestational age, preterm birth, caesarean section, low birth weight, small gestational age, and large gestational age.Results:For women aged ≥35 years, the live birth rate in PGT-A group was significantly higher than that in control group [38.0% (89/234) vs. 26.8% (237/885), OR(95% CI)=1.49(1.13-1.97), P=0.047], the miscarriage rate was significantly lower than that in control group [17.6% (19/108) vs. 29.0% (116/443), OR(95% CI)=0.45(0.24-0.85), P=0.013]. We found that for women who aged ≥38 years, the live birth rate [ OR(95% CI)=3.01(1.67-5.44), P<0.001], hCG positivity rate [ OR(95% CI)=2.08(1.25-3.47), P=0.005], clinical pregnancy rate [ OR(95% CI)=2.39(1.40-4.07), P=0.001] in PGT-A group were significantly higher than those in control group, and the miscarriage rate in PGT-A group was significantly lower than that in control group [ OR(95% CI)=0.34(0.13-0.85), P=0.022]; for women aged 35-37 years, there were no statistically significant differences in pregnancy outcomes between the two groups (all P>0.05). Moreover, there were no statistically significant differences in the rates of obstetric complications and perinatal outcomes for women aged ≥35 years between PGT-A group and control group (all P>0.05), and similar results were found in the subgroup analyses for women who aged 35-37 years or ≥38 years. Conclusion:PGT-A using blastocyst stage biopsy strategy in single blastocyst thaw transferred cycles could significantly improve the rates of hCG positivity, clinical pregnancy, and live birth, and significantly reduce the miscarriage rate for women aged ≥38 years, but could not improve the pregnancy outcomes for women aged 35-37 years. In addition, the use of PGT-A does not increase the risk of obstetric complications and perinatal outcomes for women of advanced age.

Objective:To investigate the current status of cognitive frailty among elderly patients in Urumqi and to identify its influencing factors.Methods:From March to December 2019, the elderly from 3 tertiary hospitals′ geriatrics centers in Urumqi were recruited using the general information questionnaire, FRAIL Scale, Montreal Cognitive Assessment and Clinical Dementia Rating.Results:A total of 1 006 elderly patients were surveyed, among which, 131(13.0%) cases were deemed to have developed cognitive frailty. The results of multivariate logistic regression analysis showed that age, depression, Instrumental Activities of Daily Living Scale score and diabetes were influencing factors of cognitive frailty ( P<0.001). Conclusion:The prevalence rate of cognitive frailty in elderly patients is relatively high. Medical staff should attach great importance to the assessment of cognitive frailty in elderly patients and take targeted intervention in time to prevent, slow down or reverse the onset and development of cognitive frailty.

Objective:To analyze the clinical effect of micronutrient-assisted treatment for pulmonary tuberculosis.Methods:The databases including China National Knowledge Infrastructure (CNKI) , Wanfang Data, Chinese Biomedical Literature Database, PubMed, Web of Science and The Cochrane Library were searched by computer. The literature search time limit was from the establishment of the database to December 2019. Two researchers strictly followed the inclusion and exclusion criteria to evaluate the quality of the literatures, and then used RevMan 5.3 software to perform Meta-analysis.Results:A total of 12 randomized controlled trials (RCT) were included. Meta-analysis results showed that the effect of vitamin A on the hemoglobin level of patients was statistically significantly different from that of placebo [ MD=0.46, 95% CI (0.14, 0.77) , P=0.01]. Compared with placebo, the effect of zinc on hemoglobin level was statistically significantly different [ MD=0.32, 95% CI (0.04, 0.61) , P=0.03]. Compared with placebo, the effect of zinc on mortality was statistically significantly different [ RD=0.06, 95% CI (0.01, 0.11) , P=0.02]. Compared with the control group, there was no statistically significant difference in micronutrients in negative conversion rates of tuberculosis sputum smear ( P> 0.05) . Conclusions:The intake of micronutrients can increase the hemoglobin level and reduce the fatality rate of patients with pulmonary tuberculosis, but it has little influence on the negative conversion rate of tuberculosis sputum smear.

Objective: To establish a new standard for the quality evaluation of Schisandrae chinensis Fructus. Methods: On the basis of the 2015 edition pharmacopoeia standard of Schisandrae chinensis Fructus, some testing items including the characteristic chro-matogram (HPLC) and total lignans content (UV) were established. Results: With schizandrin as the reference material, the HPLC specific chromatograms of Schisandrae chinensis Fructus including 7 characteristic peaks were established. The content of total lignans in Schisandrae chinensis Fructus was not less than 1. 8% with schizandrin as the standard. Conclusion: The proposed quality evalua-tion standard is more comprehensive and reproducible. It can provide a more scientific evaluation tool for the quality evaluation of Schisandrae chinensis Fructus and its related preparations.

Objective To investigate the expressions of minichromosome maintenance protein 5 (MCM5)and P1 6 INK4A gene and protein in cervical squamous cell cancer (SCC),cervical intraepithelial neoplasia (CIN)and normal tissues.Methods Real-time PCR and immunohistochemistry were used respectively to detect the expression levels of mRNA and protein of MCM5 and P1 6 INK4A in 40 cases of cervical cancer,1 1 cases of CIN I,1 5 cases of CIN Ⅱ - Ⅲ and 1 5 cases of normal cervical tissues.Results ① The expression levels of mRNA and protein of MCM5 and P1 6 INK4A in tissues of cervical squamous cell cancer were significantly higher than those in normal cervical tissues,CIN I and CIN Ⅱ-Ⅲ tissues (P <0.05).The expression level of P1 6 INK4A was significantly higher in tissues of cervical squamous cell cancer than in normal cervical tissues and CIN I tissues (P < 0.01 ). However,it did not differ from that in CIN Ⅱ-Ⅲ tissues (P >0.05 ).② The high expression of MCM5 mRNA and protein in cervical squamous cell carcinoma was positively correlated with clinical stage and differentiation grade of cervical cancer (P <0.01),but not correlated with age (P >0.05).P1 6 INK4A expression exhibited no correlation with clinical stage or age of patients (P >0.05),but positive correlation with differentiation grade (P <0.01).③ A positive correlation was found between MCM5 and P1 6 INK4A expressions in cervical cancer (r = 0.538,P <0.01).Conclusion The over-expressions of MCM5 and P1 6 INK4A may play an important role in the carcinogenesis and progression of cervical cancer.MCM5 may be used as a new marker of the proliferation of cervical cancer. Detection of P1 6 INK4A and MCM5 is of great significance to studying the grade and outcome of CIN,which may improve the rate of cervical cancer screening.

Objective To identify the influencing factors of the subjective well-being of geriatric patients with fractures and to provide a theoretical basis for targeted nursing interventions .Methods The Memorial University of Newfoundland Scale of Happiness ( MUNSH ) , Social Support Rating Scale ( SSRS ) , Medical Coping Modes Questionnaire ( MCMQ ) and general questionnaire were used to survey 45 geriatric patients with fractures within one-week after hospitalization .Results The score of subjective well -being was (25.77 ±12.03 ) in the participants.Among the targeted group of geriatric patients with fractures , the subjective well-being was positively correlated with subjective support and objective support (r=0.547, 0.649, respectively;P<0.05), and negatively correlated with the resignation coping style (r=-0.707,P<0.05). The subjective well-being was negatively correlated with the factors of spouse and living conditions ( r =-0.311, -0.347, respectively;P<0.05), while it was positively correlated with income ( r=0.535,P<0.01).Conclusions Favorable social support and coping style , sound family structure and living conditions as well as relatively high income will help to improve subjective well-being of geriatric patients with fractures . Nurses should provide targeted nursing intervention , while the society and families should care the elderly .

The mechanisms leading to the occurrence of seizures in humans are still poorly understood. It is widely accepted, however, that the process of seizure generation is closely associated with an abnormal synchronization of neurons. In order to investigate this process, we have studied synchronization between different regions of the brain from intracranial EEG recordings of Pilocarpine-induced epileptic rat by Synchronization likelihood (SL). It was found that during the state of transition from non-epileptiform discharges to continuous-epileptiform discharges, synchronization between left-ECoG and left-EHG was significantly strengthened, and similar change had taken place between right-ECoG and right-EHGd; left-ECoG; and right-ECoG and left-EHG and right-EHG (P < 0.05). During the state of transition from continuous-epileptiform discharges to period-epileptiform discharges, the synchronization was significantly weakened between left-ECoG and left-EHG, and similar change was noted between left-EHG and right-EHG (P < 0.01). The results showed that SL might be used to assess the dynamics of synchronization and it might be helpful to understanding the mechanisms involving the origin of epileptiform discharge.
Animals ; Cerebral Cortex ; physiopathology ; Electroencephalography ; methods ; Epilepsy ; chemically induced ; physiopathology ; Hippocampus ; physiopathology ; Male ; Pilocarpine ; Rats ; Rats, Sprague-Dawley ; Signal Processing, Computer-Assisted