Objective To assess the intervention effect of nicotinamide mononucleotide (NMN) on the mouse model of hematotoxicity induced by subacute benzene exposure. Methods Benzene exposure and NMN intervention were adopted in a 2×2 factorial design, as benzene exposure and non-exposure, and NMN intervention and non-intervention. Male specific pathogen-free C57BL/6J mice were randomly assigned to negative control group, NMN control group, simple benzene exposure group and NMN intervention group, with 12 mice in each group. Benzene exposure of mice in simple benzene exposure group and NMN intervention group was conducted by dynamic inhalation of benzene at a concentration of 325 mg/m³ for six hours per day, five days per week for four weeks (28 days). Mice in the negative control and NMN control group inhaled clean air. During benzene exposure, mice in the NMN control group and NMN intervention group received NMN in drinking water at a dose of 300 mg/kg body weight. Peripheral blood samples of mice were collected for complete blood count analysis and calculation of composite inflammatory indices after 28 days. Results Interaction analysis showed that the counts of peripheral white blood cell, neutrophil, lymphocyte, and platelet of mice in the simple benzene exposure group were lower than those in the negative control group (all P<0.05). Neutrophil and platelet counts in the NMN intervention group were higher than those in the simple benzene exposure group (all P<0.05). The results of main effect analysis showed that the monocyte count of peripheral blood, systemic inflammatory index, systemic inflammatory response index, neutrophil/lymphocyte ratio and platelet/lymphocyte ratio of mice in the benzene exposure group increased (all P<0.05), and the basophil count and lymphocyte/monocyte ratio decreased (all P<0.05), compared with the control group. Conclusion Oral NMN alleviates subacute benzene-induced decreases in peripheral neutrophil and platelet counts in mice. This protective effect may be related to the targeted intervention of NMN on mitochondrial energy metabolism disorder and oxidative damage induced by benzene exposure in male mice.

Objective To investigate the epidemiological characteristics and influencing factors of Femoral Artery Compression-Related Skin Complications Around the Puncture Site(FACR-SCAPS)in patients with primary hepatocellular carcinoma(HCC)undergoing transarterial chemoembolization(TACE).Methods A multicenter cross-sectional study was conducted using convenience sampling.A total of 1 573 HCC patients who underwent TACE between April 2023 and October 2024 were recruited from interventional radiology departments,oncology units,and specialized centers across 10 hospitals in Beijing,Tianjin,Shandong,Hebei,Qinghai,and Inner Mongolia.Descriptive statistics,univariate analysis,and multivariate logistic regression were used to explore the epidemiological characteristics and influencing factors of FACR-SCAPS in this population.Results Among the 1 573 primary HCC patients undergoing TACE interventional therapy,FACR-SCAPS occurred in 28.99％(456/1 573),with a total of 476 complication instances recorded(30.26 per 100 patients).Patients with a single complication accounted for 96.93％,whereas those with multiple complications constituted 3.07％.The most prevalent types of complications were skin erythema,skin ecchymosis,and hard lumps formation,collectively accounting for 96.49％of all complications.Hematoma,blisters,and rupture complications collectively accounted for only 4.61％.Logistic regression analysis revealed that peak diastolic blood pressure during compression(OR=1.024,95％CI:1.013-1.035,P＜0.001),use of rotary compression hemostasis devices(OR=3.220,95％CI:2.120-4.891,P＜0.001),elevated PT-INR(OR=19.630,95％CI:6.039-63.810,P＜0.001),and anticoagulant use within the last three months(OR=1.909,95％CI:1.064-3.427,P=0.030)were significant influencing factors associated with FACR-SCAPS post-TACE.Conclusion FACR-SCAPS is commonly seen among primary HCC patients after TACE,its risk factors include peak diastolic blood pressure during compression,use of rotary compression devices,elevated PT-INR,and recent anticoagulant use.

Objective To explore the CT diagnosis and differential diagnosis of perivascular epithelioid cell neoplasms (PEComa), improve the accuracy of PEComa diagnosis, and reduce misdiagnosis. Methods CT findings of 8 cases of PEComa confirmed by pathology in Jining First People’s Hospital from January 2020 to April 2024 were retrospectively analyzed for the location, shape, size, boundary, plain scan density, and enhancement characteristics of the lesions. Results All 8 tumors were solitary, with 5 located in the kidney, 1 in the liver, 1 in the extraperitoneal space, and 1 in the retroperitoneal space. The tumors were round in 3 cases, oval in 1 case, and irregular in 4 cases. Seven cases were benign with clear boundaries and 1 case was malignant with unclear boundaries. On plain CT, 2 cases showed slightly low density, 3 cases showed slightly high density, and 3 cases showed low density. One tumor had uniform density, and 7 tumors had nonuniform density with internal necrosis and cystic changes. Contrast-enhanced CT revealed diverse enhancement patterns. Four cases showed a “fast in and fast out” enhancement pattern, with significant arterial-phase enhancement and reduced portal venous-phase enhancement. Three cases showed a “fast-in and slow-out” enhancement pattern, with significant enhancement in the arterial phase, persistent enhancement in the portal venous phase, and slightly reduced density in the delayed phase. One case showed mild enhancement in the arterial phase and significant enhancement in the portal venous phase. In 3 cases, multiple tortuous and thickened blood vessels were observed around the tumors, while 3 cases showed tortuous vascular shadows within the tumors. Conclusion PEComa demonstrates characteristic CT features, predominantly with “fast in and fast out” or “fast in and slow out” enhancement patterns. When thickened and tortuous blood vessels are observed within or around the tumor, PEComa should be considered in combination with clinical findings.

Objective:To analyze the correlation of ultrasonic shear wave elastography (SWE) and ultrasonic contrast with different molecular types of breast cancer was analyzed.Methods:A total of 100 patients with breast cancer admitted to our hospital from Jun. 2019 to Jun. 2024 were included, and general data of patients were collected. Patients were divided into lumen type, human epidermal growth factor receptor 2 (HER2) positive type and triple-negative type according to molecular classification. SWE imaging parameters [mean elastic modulus (Emean), maximum elastic modulus (Emax), standard deviation (Esd) ], quantitative parameters of contrast-enhanced ultrasound [peak intensity (PI), time to peak (TTP) ], qualitative parameters of ultrasonic contrast (enhancement speed, enhancement intensity, lesion expansion after enhancement, lesion margin, perfusion defect, vascular penetration) were compared. The correlation of ultrasonic SWE parameters, ultrasonic contrast parameters with different molecular types of breast cancer was analyzed.Results:Among 100 breast cancer patients, 48 were luminal type, 34 were HER2 overexpression type, and 18 were triple-negative type. There was no statistically significant difference in age, tumor diameter, tumor location, case cytology type, Emean, Esd, TTP, lesion expansion after enhancement, lesion margin, perfusion defect, or vascular penetration among three groups ( F=0.21, 1.28, 2.47, 0.91, x2=2.29, 0.62, 0.03, 4.88, 0.96, 0.64, 3.10, P > 0.05). Pathological grade, Emax, enhancement speed, enhancement intensity and PI among the three groups showed statistically significant differences, and the Emax and PI of luminal patients were significantly lower than those of HER2 overexpression type and triple-negative type ( χ2=13.28, 24.06, 17.90, F=11.65, 5.75, P < 0.05) ; Emax, enhancement velocity, enhancement intensity and PI in ultrasonic SWE parameters of breast cancer patients were correlated with different molecular types ( r=0.37, 0.40, 0.52, 0.32, P < 0.05) . Conclusions:Ultrasonic SWE and ultrasonic contrast have differences in different molecular types of breast cancer. There were significant differences in Emax, enhancement speed, enhancement intensity and PI among patients with different molecular types. The Emax and PI of HER2 overexpression type and triplenegative type are significantly higher, and Emax, enhancement speed, enhancement intensity and PI are significantly correlated with molecular typing of breast cancer.

Objective:To analyze the correlation of ultrasonic shear wave elastography (SWE) and ultrasonic contrast with different molecular types of breast cancer was analyzed.Methods:A total of 100 patients with breast cancer admitted to our hospital from Jun. 2019 to Jun. 2024 were included, and general data of patients were collected. Patients were divided into lumen type, human epidermal growth factor receptor 2 (HER2) positive type and triple-negative type according to molecular classification. SWE imaging parameters [mean elastic modulus (Emean), maximum elastic modulus (Emax), standard deviation (Esd) ], quantitative parameters of contrast-enhanced ultrasound [peak intensity (PI), time to peak (TTP) ], qualitative parameters of ultrasonic contrast (enhancement speed, enhancement intensity, lesion expansion after enhancement, lesion margin, perfusion defect, vascular penetration) were compared. The correlation of ultrasonic SWE parameters, ultrasonic contrast parameters with different molecular types of breast cancer was analyzed.Results:Among 100 breast cancer patients, 48 were luminal type, 34 were HER2 overexpression type, and 18 were triple-negative type. There was no statistically significant difference in age, tumor diameter, tumor location, case cytology type, Emean, Esd, TTP, lesion expansion after enhancement, lesion margin, perfusion defect, or vascular penetration among three groups ( F=0.21, 1.28, 2.47, 0.91, x2=2.29, 0.62, 0.03, 4.88, 0.96, 0.64, 3.10, P > 0.05). Pathological grade, Emax, enhancement speed, enhancement intensity and PI among the three groups showed statistically significant differences, and the Emax and PI of luminal patients were significantly lower than those of HER2 overexpression type and triple-negative type ( χ2=13.28, 24.06, 17.90, F=11.65, 5.75, P < 0.05) ; Emax, enhancement velocity, enhancement intensity and PI in ultrasonic SWE parameters of breast cancer patients were correlated with different molecular types ( r=0.37, 0.40, 0.52, 0.32, P < 0.05) . Conclusions:Ultrasonic SWE and ultrasonic contrast have differences in different molecular types of breast cancer. There were significant differences in Emax, enhancement speed, enhancement intensity and PI among patients with different molecular types. The Emax and PI of HER2 overexpression type and triplenegative type are significantly higher, and Emax, enhancement speed, enhancement intensity and PI are significantly correlated with molecular typing of breast cancer.

Objective:To observe the clinical effect of Traditional Chinese Medicine (TCM) collapsibility combined with pressure therapy in the treatment of eczema.Methods:From January 2021 to August 2021, 60 patients with lower extremity venous ulcers who were treated in Wangjing Hospital, China Academy of Chinese Medical Sciences were selected and randomly divided into the control group (30 patients) and observation group (30 patients). The control group was treated by the routine pressure therapy, and the observation group was by the TCM nursing intervention on the basis of the control group. Both groups were treated for 2 weeks. The visual analogy scale (VAS),chronic Venous Insufficiency Questionnaire (CIVIQ), Self-rating Anxiety Acale (SAS), and Self-rating Depression Scale (SDS) were measured and evaluated at baseline and after treatment.Results:The total response rate was 100.0% (30/30) in the observation group and 86.7% (26/30) in the control group, and the difference was statistically significant ( χ2=4.29, P=0.038). The VAS (2.47±0.68 vs. 2.97±0.14, t=3.95), SAS (40.31±11.93 vs. 55.87±4.53, t=4.73), SDS (44.30±5.38 vs. 47.61±5.33, t=2.39) scores were significantly lower than those in the control group ( P<0.05), CIVIQ score (86.63±2.74 vs. 84.67±3.23, t=2.54) was significantly higher than that of the control group ( P<0.05). Conclusion:TCM combined with pressure therapy can promote the healing of ulcers in patients with eczema, relieve pain, and improve the quality of life of patients.

OBJECTIVE: To analyze the relationship between hemoglobin(Hb) level on admission and survival prognosis of patients with hip fracture. METHODS: From February 2016 to October 2018, 249 elderly patients with hip fracture were surgically treated including 62 males and 187 females;the age ranged from 60 to 91(73.67±10.52) years;the time from injury to operation was (6.79±2.27) d. The clinical and laboratory examination results were collected. The Hb level at admission and the mortality at 30, 90, 180 and 360 days after operation were observed. According to the Hb level at admission, the patients were divided into Hb<120 g/L and Hb≥120 g/L groups. The survival conditions of the two groups at 30, 90, 180 and 360 days after operation were compared and analyzed. Logistic regression was used to analyze the effect of Hb level on death 30, 90, 180 and 360 days after operation. RESULTS: The mortality rates at 30, 90, 180 and 360 days after operation were 5.22%, 9.24%, 16.87% and 20.48% respectively. The level of Hb at admission was a risk factor for prognosis and death 30, 90, 180 and 360 days after operation(P<0.05). The OR(95% CI) were 2.431(1.475-4.006), 2.625(1.468-4.695), 2.276(1.320-3.925) and 2.082(1.221-3.551) respectively. CONCLUSION The level of Hb at admission can affect the survival and prognosis of elderly patients with hip fracture. We should further study how to manage the level of Hb before operation.
Aged ; Aged, 80 and over ; Female ; Hemoglobins/analysis* ; Hip Fractures/surgery* ; Hospitalization ; Humans ; Male ; Middle Aged ; Prognosis ; Retrospective Studies ; Risk Factors

Objective:To screen the biomarkers in the exhaled breath of mice exposed to benzene by using exhaled breath online analysis system.Methods:Thirty 8-week-old male C57BL/6 mice were randomly divided into six groups (0, 3, 32, 324, 648, and 1 296 mg/m 3) and treated with benzene vapour for 28 days. At the end of the exposure, the peripheral blood cell counts and blood glutathione (GSH) were detected. The content of malondialdehyde (MDA) in HL60 cells treated by mice plasma was examined. Exhaled breath data from mice were collected by Secondary electrospray ionization source high resolution mass spectrometry (SESI-HRMS). Targeted analysis underlying benzene metabolites and oxidative stress metabolites was performed to screen the biomarkers in exhaled breath. Results:After benzene exposure, the number of peripheral blood cells was decreased in different degrees, particularly in the white blood cells (WBC) number. The WBC in 32 and 324 mg/m 3 groups was declined by 27.76% and 52.87%, respectively compared to that in control group ( P<0.05). Meanwhile, compared with the control group, the GSH content of peripheral blood cells from 324 mg/m 3 group decreased by 13.16% ( P<0.05). In addition, MDA content was increased by 18.11% in HL60 cells treated with plasma from 324 mg/m 3 group mice ( P<0.05). The phenol, hydroquinone/catechol, benzenetriol and trans, trans-Muconic acid ( t,t-MA) in the exhaled gas of mice could be used as biomarkers for benzene exposure ( R 2>0.8, P<0.001). The peak intensity of five small molecular metabolites related to oxidative stress (ω-carboxylic fatty acid C 5H 10O 3, ω-carboxylic fatty acid C 6H 12O 3, glutamate, cysteine and MDA) increased with the increase of benzene concentration ( P<0.05), which was negatively correlated with WBC decline ( P<0.001), suggesting that these molecules mignt be used as biomarkers of benzene-induced toxicity. Conclusions:Phenol, hydroquinone/catechol, benzenetriol and trans, trans-Muconic acid ( t,t-MA) in exhaled breath of mice could be used as biomarkers for benzene exposure; ω-carboxylic fatty acid C 5H 10O 3, ω-carboxylic fatty acid C 6H 12O 3, glutamate, cysteine and MDA might be used as markers of benzene-induced toxicity.

Objective:To screen the biomarkers in the exhaled breath of mice exposed to benzene by using exhaled breath online analysis system.Methods:Thirty 8-week-old male C57BL/6 mice were randomly divided into six groups (0, 3, 32, 324, 648, and 1 296 mg/m 3) and treated with benzene vapour for 28 days. At the end of the exposure, the peripheral blood cell counts and blood glutathione (GSH) were detected. The content of malondialdehyde (MDA) in HL60 cells treated by mice plasma was examined. Exhaled breath data from mice were collected by Secondary electrospray ionization source high resolution mass spectrometry (SESI-HRMS). Targeted analysis underlying benzene metabolites and oxidative stress metabolites was performed to screen the biomarkers in exhaled breath. Results:After benzene exposure, the number of peripheral blood cells was decreased in different degrees, particularly in the white blood cells (WBC) number. The WBC in 32 and 324 mg/m 3 groups was declined by 27.76% and 52.87%, respectively compared to that in control group ( P<0.05). Meanwhile, compared with the control group, the GSH content of peripheral blood cells from 324 mg/m 3 group decreased by 13.16% ( P<0.05). In addition, MDA content was increased by 18.11% in HL60 cells treated with plasma from 324 mg/m 3 group mice ( P<0.05). The phenol, hydroquinone/catechol, benzenetriol and trans, trans-Muconic acid ( t,t-MA) in the exhaled gas of mice could be used as biomarkers for benzene exposure ( R 2>0.8, P<0.001). The peak intensity of five small molecular metabolites related to oxidative stress (ω-carboxylic fatty acid C 5H 10O 3, ω-carboxylic fatty acid C 6H 12O 3, glutamate, cysteine and MDA) increased with the increase of benzene concentration ( P<0.05), which was negatively correlated with WBC decline ( P<0.001), suggesting that these molecules mignt be used as biomarkers of benzene-induced toxicity. Conclusions:Phenol, hydroquinone/catechol, benzenetriol and trans, trans-Muconic acid ( t,t-MA) in exhaled breath of mice could be used as biomarkers for benzene exposure; ω-carboxylic fatty acid C 5H 10O 3, ω-carboxylic fatty acid C 6H 12O 3, glutamate, cysteine and MDA might be used as markers of benzene-induced toxicity.

Objective:To evaluate the clinical value of three-dimensional ultrasound imaging in the localization and shaping of intrauterine contraceptive device.Methods:A total of 46 patients with abnormal intrauterine contraceptive device positions who admitted to Xin'an International Hospital from May 2018 to May 2019 were selected.All patients were examined by two-dimensional ultrasound and three-dimensional ultrasound, and the results were compared with the surgical results of the patients.The application value of three-dimensional ultrasound in the localization and shaping of intrauterine contraceptive device was analyzed.Results:By surgery pathology diagnosed with synthetic in palace in 2 cases, synthetic down in 29 cases, synthetic part or security in 15 cases.The two-dimensional ultrasonic diagnosis of patients with synthetic in the palace in 2 cases, synthetic down in 30 cases, synthetic part or security embedded in 11 cases, abnormal suspicious uncertain in 3 cases, 3 cases of missed diagnosis, 1 case of misdiagnosis.The three-dimensional ultrasound diagnosis in patients with synthetic in the palace in 2 cases, synthetic down in 29 cases, synthetic part or security embedded in 14 cases, abnormal suspicious uncertain in 1 case, no misdiagnosis, missed diagnosis in 1 case, pure synthetic down patients can be removed under hysteroscopy.Patients with IUD outside the uterus were removed laparoscopically without IUD rupture or residual fragments.And synthetic guided by hysteroscopy in patients with incarcerated during extraction, 3 cases of synthetic fracture or residual fragments occurring and compared with synthetic down the difference was statistically significant (χ 2=4.982, P<0.05), compared with the synthetic located outside the uterus, the difference was statistically significant (χ 2=4.634, P<0.05), the incidence of completely bypassed economizer fracture or residual fragments was higher than partial incarceration patients (χ 2=4.845, P<0.05). Conclusion:Three-dimensional ultrasound has higher accuracy in positioning and shaping the intrauterine contraceptive device of patients, which can guide clinicians to choose the treatment program.