To determine the main components of the fishy smell of the Eupolyphaga Steleophaga, and to provide a theoretical basis for deodorizing the Eupolyphaga Steleophaga. METHODS Head space-solid phase microextraction-gas chromatography-mass spectrometry was used to identify the components of 10 batches of Eupolyphaga Steleophaga, and area normalization method and chemometrics method were used to analyze the smelly gas of different batches. Odor activity value(OAV) was used to evaluate the contribution of odor components and identify key odor components. RESULTS A total of 87 volatile odor components were identified, the key fishy smell compounds(OAV≥1) were m-methylphenol, dimethyltrisulfide, 4-methylphenol, 2-methyliso-borneol, 2-etzol, 4-methylvaleric acid, iso-valeric acid, etc. Modified fishy gas composition(0.1

Objective To observe the clinical efficacy of chaizhu liwei yin combined with domperidone in the treatment of functional dyspepsia(FD)with spleen deficiency and qi stagnation stype accompanied by sleep disorders.Methods A total of 80 elderly patients with FD with spleen deficiency and qi stagnation stype accompanied by sleep disorders in the Geriatric Clinic,Nangang Hospital of Hei-longjiang Academy of Chinese Medicine from September 2020 to September 2022 were selected as the research objects,and they were ran-domly divided into the observation group and the control group,with 40 cases in each group.The observation group was treated with domp-eridone tablets combined with chaizhu liwei yin,while the control group was treated with domperidone tablets,the patients in the two groups were given continuous administration for 4 weeks.The clinical efficacy,TCM syndrome efficacy evaluation score,Nepean dyspep-sia index(NDI)and Pittsburgh sleep quality index(PSQI)were observed before and after treatment in the two groups of elderly FD pa-tients.Results During the treatment,1 case was shed in the control group and 2 cases in the observation group.77 patients were includ-ed in the curative effect observation statistics,including 39 cases in the control group and 38 cases in the observation group.After treat-ment,the total effective rate of clinical symptoms in the observation group and the control group was 86.84％and 69.23％,respectively,and the observation group was better than the control group(P＜0.05).the scores of TCM syndrome efficacy evaluation in observation group and control group were lower than before treatment(P＜0.05),and the observation group was lower than the control group(P＜0.05);NDI scores of the observation group(77.13±5.62)and control group(61.03±5.33)were both improved compared with before treatment(P＜0.05),and the improvement of the observation group was higher than that of the control group(P＜0.05);the total scores of PSQI in the observation group(5.23±1.52)and control group(7.74±2.17)were lower than before treatment(P＜0.05),and the total scores of PSQI in the observation group was lower than that in the control group(P＜0.05);During the observation period,there were no obvious adverse reactions in the observation group and the control group,indicating the safety was high.Conclusion Chai liwei yin combined with domperidone can effectively relieve gastrointestinal symptoms in elderly FD patients with spleen deficiency and qi stagnation stype,improve sleep status and improve the quality of life in elderly patients.

OBJECTIVES: Application of ultrashort wave (USW) to rats with cerebral ischemia and reperfusion injury could inhibit the decrease of expression of secretory pathway Ca²⁺-ATPase 1 (SPCA1), an important participant in Golgi stress, reduce the damage of Golgi apparatus and the apoptosis of neuronal cells, thereby alleviating cerebral ischemia-reperfusion injury. This study aims to investigate the effect of USW on oxygen-glucose deprivation/reperfusion (OGD/R) injury and the expression of SPCA1 at the cellular level. METHODS: N2a cells were randomly divided into a control (Con) group, an OGD/R group, and an USW group. The cells in the Con group were cultured without exposure to OGD. The cells in the OGD/R group were treated with OGD/R. The cells in the USW group were treated with USW after OGD/R. Cell morphology was observed under the inverted phase-contrast optical microscope, cell activity was detected by cell counting kit-8 (CCK-8), apoptosis was detected by flow cytometry, and SPCA1 expression was detected by Western blotting. RESULTS: Most of the cells in the Con group showed spindle shape with a clear outline and good adhesion. In the OGD/R group, cells were wrinkled, with blurred outline, poor adhesion, and lots of suspended dead cells appeared; compared with the OGD/R group, the cell morphology and adherence were improved, with clearer outlines and fewer dead cells in the USW group. Compared with the Con group, the OGD/R group showed decreased cell activity, increased apoptotic rate, and down-regulating SPCA1 expression with significant differences (all P<0.001); compared with the OGD/R group, the USW group showed increased cell activity, decreased apoptotic rate, and up-regulating SPCA1 expression with significant differences (P<0.01 or P<0.001). CONCLUSIONS USW alleviates the injury of cellular OGD/R, and its protective effect may be related to its up-regulation of SPCA1 expression.
Animals ; Rats ; Apoptosis ; Brain Ischemia ; Glucose/metabolism* ; Oxygen/metabolism* ; Reperfusion Injury/metabolism* ; Transcriptional Activation ; Up-Regulation ; Calcium-Transporting ATPases/metabolism*

Objective:To automatically and rapidly detect mild cognitive impairment (MCI) in an objective manner using natural language processing (NLP).Methods:A total of 215 participants (half female) aged 50 to 80 were recruited for the study′s normal cognition and MCI groups. Speech tasks and the mini mental state examination (MMSE-2) were used to collect audio data and quantify cognitive functioning. Altogether 162 acoustic features were extracted including the speaking speed, syllable number, syllable duration, number of pauses, duration of pauses, the standard deviation of formant frequency and sound pressure variation. They were compared between the two groups and genders. Multiple regression analysis was used to formulate a model predicting MCI. The sensitivity, specificity and accuracy of its predictions were used to evaluate its predictive power.Results:There were significant differences between the two groups in 50 acoustic features including their pronunciation rhythm and pronunciation accuracy. Univariate correlation analysis revealed that the pronunciation rhythm was significantly associated with cognitive functioning. The sensitivity, specificity and accuracy of the model were 0.54, 0.80 and 0.69 for males and 0.00, 0.86 and 0.63 for females.Conclusion:MCI greatly affects pronunciation rhythm. Acoustic analysis based on NLP can detect MCI rapidly and objectively.

Objective: To analyze the association between overweight, obesity and high blood pressure among middle school students in Kaifeng city. Methods: A multi stage stratified cluster sampling method was used to select 4 058 middle school students in Kaifeng City to measure blood pressure, height and weight, and to analyze the relationship between overweight and obesity and high blood pressure from September to October 2019. Results: The prevalence of high blood pressure in middle school students in Kaifeng was 11.78%. Compared with normal weight group, overweight ( OR =1.65) and obesity ( OR =3.32) were significantly correlated with the risk of high blood pressure ( P <0.01). The risk of high blood pressure in overweight and obese boys was 1.87 and 4.16 times higher than that in normal weight boys, respectively. The risk of high blood pressure for overweight and obese girls was 1.55 and 2.69 times higher than that for normal weight girls, respectively. The risk of high blood pressure in high school and normal weight group, middle school and overweight/obesity group, and high school and overweight/obesity group were 1.90, 2.85 and 3.39 times higher than that in middle school and normal weight group, respectively ( P =0.02). Conclusion The prevention of high blood pressure in middle school students should start from the prevention of overweight and obesity, especially among overweight and obese boys. Risk of high blood pressure increases with grade among overweight/obese middle school students.

Objective:To translate Fear of Progression Questionnaire (FoP-Q) into Chinese and test its reliability and validity.Methods:After translation, back translation and cultural adaptation, the items of the simplified Chinese version of FoP-Q were determined. Using the convenient sampling method, a total of 269 patients with hematological malignancies from two Class Ⅲ Grade A hospitals in Beijing were selected as the research objects from August to December 2020. The general information questionnaire and the simplified Chinese version of FoP-Q were used to investigate the patients. Exploratory factor analysis was used to evaluate the structural validity of the simplified Chinese version of FoP-Q. Cronbach 's α coefficient and half-fold reliability coefficient were used to evaluate the reliability of the scale. Pearson correlation analysis method was used to analyze the correlation between each dimension and between each dimension and the total scale. A total of 269 questionnaires were distributed, and 256 valid questionnaires were returned. The effective response rate was 95.167% (256/269) . Results:The simplified Chinese version of FoP-Q extracted 5 factors through factor analysis, with a total of 41 items, and the cumulative variance contribution rate was 64.201%. The content validity index of each item of the scale was 0.857-1.000, the Kappa value was 0.850-1.000, the content validity index of the overall consistency scale was 0.814 and the content validity index of the average scale was 0.973. The total Cronbach 's α coefficient of the scale was 0.969, and the Cronbach 's α coefficient of the five factors was 0.815-0.941. The half-fold reliability coefficient of the total scale was 0.955. Conclusions:The simplified Chinese version of Fear of Progression Questionnaire has good reliability and validity, which can be used to evaluate the degree of fear of disease progression in patients with malignant tumors in China

Objective: To understand the prevalence and influencing factors of depressive of middle school students in Kaifeng City, and to provide evidence for the prevention and control of depressive symptoms among middle school students. Methods: A multistage stratified cluster random sampling method was used to select 4 058 middle school students in Kaifeng City from September to November 2019. A questionnaire survey was conducted using the Center for Epidemiologic Studies Depression Scale (CES-D). Results: The prevalence of depressive symptoms among middle school students in Kaifeng City was 14.51%. The results of multivariable Logistic analysis showed that the risk of depressive symptoms in high school students was significantly higher than that of middle school students( OR =1.47). Call in sick, leaving school due to illness, serious injury, school bullying, fighting, domestic violence, smoking and internet addiction were positively associated with depressive symptoms( OR =1.37，1.67，1.91，2.69， 1.65 ，2.29，1.47，2.58， P <0.05). In addition, the interaction of fighting and smoking was associated with more depressive symptoms in middle school students. Conclusion The depression of middle school students is closely related to grade, family and campus environment. Developing a positive family and school environment, preventing adverse events in daily life are necessary measures to reduce the risk of depression symptoms for students.

OBJECTIVES: To examine the changes of coenzyme Q10 (CoQ10) and β-galactosyl transferase specific chaperone 1 (C1GALT1C1) in brain of rats with ischemic injury at different time points and to explore the protective mechanism of ultrashort wave (USW) on ischemic brain injury. METHODS: Fifty SD rats were randomly divided into 5 groups (=10 per group): a sham group (control group) and 4 experimental group (ischemia for 2 h). The 4 experimental groups were set as a model 1 d group, a USW 1 d group, a model 3 d group and a USW 3 d group, respectively. Five rats were randomly selected for 2,3,5-triphenyltetrazoliumchloride (TTC) staining in each experimental group, and the remaining 5 rats were subjected to Western blotting and real-time PCR. The percentage of cerebral infarction volume and the relative expression level of CoQ10 and C1GALT1C1 in the brain were examined and compared. RESULTS: The infarct volume percentage after TTC staining was zero in the sham group. With the progress of disease and USW therapy, the infarct volume percentage was decreased in the experimental groups (all <0.05); Western blotting and real-time PCR showed that the relative expression level of CoQ10 in the sham group was the highest, while in the experimental groups, the content of CoQ10 showed a upward trend with the extension of disease and USW therapy, with significant difference (all <0.05). The relative expression level of C1GALT1C1 in the sham group was the lowest, but in the experimental groups, they showed a downward trend with the extension of disease and USW therapy, with significant difference (all <0.05). CONCLUSIONS Non-caloric USW therapy may upregulate the expression of CoQ10 to suppress the expression of C1GALT1C1 in rats, leading to alleviating cerebral ischemic reperfusion injury.
Animals ; Brain ; Brain Ischemia ; Molecular Chaperones ; Rats ; Rats, Sprague-Dawley ; Reperfusion Injury ; Ubiquinone ; analogs & derivatives

Objective:To observe the changes of gut flora in mice, and explore the outcome of fecal microbiota transplantation combined with probiotics in the intervention of severe acute liver injury.Methods:Forty male BALB/c mice were selected and randomly divided into blank control group (10 mice), model group (10 mice), ordinary fecal microbiota transplantation group (10 mice), and fecal microbiota + probiotics transplantation group (10 mice). An intraperitoneal injection of d-galactosamine (D-GalN 3.0g/kg) was given to every group except the blank control group to induce severe acute liver injury model. Simultaneously, ordinary fecal microbiota transplantation group and fecal microbiota + probiotics transplantation group and modeling group were given enema solutions (once a day). After 48 hours, fetched serum was taken to detect alanine transaminase, aspartate transaminase and total bilirubin, and liver tissue was taken for pathological detection. The colonic content was used to extract DNA for 16S V3-V4 high-throughput sequencing. The results of sequencing were analyzed by using bioinformatics analysis; including OTU cluster analysis, α diversity analysis, β diversity analysis, and linear discriminant analysis effect size (Lefse) to find the bacteria with different colonic content characteristics in different groups of mice. Differences in clinical biochemical indicators between groups were compared using t-test, and the differences between 16S V3-V4 region sequencing results were compared using Wilcoxon test.Results:Model group mice serum biochemical parameters were higher than the other three groups, and the difference was statistically significant ( P < 0.05). HE staining of liver sections showed severe inflammatory changes under the microscope in the model group. Ordinary fecal microbiota transplantation group and fecal microbiota + probiotic microbiota transplantation group had low levels of inflammation than the model group. The analysis results of 16S rRNA high-throughput sequencing showed that there was no statistically significant difference in Shannon’s index between the blank control and the other three groups. Observed Species difference was statistically significant, and the gut flora composition varied greatly. Species number in the mice gut flora was increased with fecal microbiota transplantation. The results of β - diversity analysis showed that the difference between the blank control group and the other three groups was greater than that between the disease groups. The difference in the structure of the gut flora of the diseased mice in the fecal microbiota + probiotic transplantation group was mostly butyrate-producing bacteria. Conclusion:Fecal microbiota + probiotics enhance the therapeutic effect of fecal microbiota transplantation, improve liver inflammation, and increase the number of butyrate-producing bacteria in the gut.