The Janus kinase/signal transducers and activators of transcription (JAK-STAT) control natural killer (NK) cells development and cytotoxic functions, however, whether long non-coding RNAs (lncRNAs) are involved in this pathway remains unknown. We found that miR155HG was elevated in activated NK cells and promoted their proliferation and effector functions in both NK92 and induced-pluripotent stem cells (iPSCs)-derived NK (iPSC-NK) cells, without reliance on its derived miR-155 and micropeptide P155. Mechanistically, miR155HG bound to miR-6756 and relieved its repression of JAK3 expression, thereby promoting the JAK-STAT pathway and enhancing NK cell proliferation and function. Further investigations disclosed that upon cytokine stimulation, STAT3 directly interacts with miR155HG promoter and induces miR155HG transcription. Collectively, we identify a miR155HG-mediated positive feedback loop of the JAK-STAT signaling. Our study will also provide a power target regarding miR155HG for improving NK cell generation and effector function in the field of NK cell adoptive transfer therapy against cancer, especially iPSC-derived NK cells.

Objective To develop a full system to monitor G-induced loss of consciousness(G-LOC)of pilots caused by continuous positive acceleration and wake up them in time,so as to shorten the airborne unconsciousness time of pilots.Methods The system used MPU6050 sensor and SICHIRAY muscle sensor to obtain the head posture and electromyogram(EMG)characteristics of the pilot's environment in real time.After system hardware design,main program design,head posture monitoring module design,EMG signal acquisition and processing module and upper computer interface display design,the system test and centrifuge test were carried out.The system test set the threshold of the EMG signal and the head posture angle by collecting the EMG signals of the subjects 2 min at rest and under muscle contraction and the head posture angle of the human body looking forward and bowing as hard as possible,and the acceleration threshold was the gravity acceleration in the test environment.The centrifuge test simulated the process of raising and bowing of the human body by placing the position of the attitude angle on the animal centrifuge.The acceleration threshold was set at 3 G,and the positive acceleration of this test environment was 4,6,and 8 G.Results The system could capture the head posture and the physiological characteristics of EMG signals in real time,send out voice reminders in time,and could immediately enter the alarm wake-up mode when failed to receive feedback(head up,EMG recovery)within fixed time.And it could be used on centrifuges.Conclusion This system has complete function,complete logic and strong practicability.It is possible for practical application after further experimental improvement.

With the development of space technology,the health risks for astronauts in space have also increased,which has made the application value of telemedicine technology in space exploration more prominent.In the outer space environment,various exogenous and endogenous factors may affect human health in multiple ways.As deep space exploration advances and space technology develops,the risks of astronauts'health issues in space increase,and telemedicine technology has important application value in space exploration,which is related to astronauts'lives,health,and the success of missions.This article summarizes the research progress of telemedicine technology in recent years,analyzes remote medical diagnosis and treatment,intelligent surgical robots,and the application of artificial intelligence,discusses the latest progress in related fields at home and abroad,and provides a reference for the medical support of long-term space flight.

In the field of space exploration,the importance of microorganisms to human health cannot be ignored,and they will play a crucial role in maintaining astronaut health,ensuring habitat sustainability,and ensuring the success of the entire mission.Microorganisms may have negative impacts on space missions,but they also have practical value.This article reviews research progress in recent years on the effects of space environments on microorganisms,the current status of microbial contamination in space environments,detection methods,and the application of microorganisms in the field of space health.It clarifies that research on the impact of microorganisms and microbial ecosystems in space exploration is not only helpful in safeguarding the survival and health of astronauts,but also provides valuable reference for future space exploration projects.

Aortic dissection (AD) is a life-threatening vascular disease due to the tearing of aortic intimal layer, leading to the formation of pseudocavity. Once the acute progression of dissection happens, serious complications such as rupture and stroke may occur. The current imaging examinations for AD are invasive and may cause adverse effects related to contrast medium, which cannot be used for large-scale screening of AD. The latest studies have found that metabolic processes and metabolites of lipids,saccarides and proteins are involved in the pathogenesis and development of AD. In this article, we review the research progress in the caracteristics of AD related metabolism,summarize changes of specific metabolites in AD,and explore the clinical implication of studies on AD related metaboliome..

Tryptophan 2,3-dioxygnease 2 (TDO2) is specific for metabolizing tryptophan to kynurenine (KYN), which plays a critical role in mediating immune escape of cancer. Although accumulating evidence demonstrates that TDO2 overexpression is implicated in the development and progression of multiple cancers, its tumor-promoting role in esophageal squamous cell carcinoma (ESCC) remains unclear. Here, we observed that TDO2 was overexpressed in ESCC tissues and correlated significantly with lymph node metastasis, advanced clinical stage, and unfavorable prognosis. Functional experiments showed that TDO2 promoted tumor cell proliferation, migration, and colony formation, which could be prevented by inhibition of TDO2 and aryl hydrocarbon receptor (AHR). Further experimentation demonstrated that TDO2 could promote the tumor growth of KYSE150 tumor-bearing model, tumor burden of C57BL/6 mice with ESCC induced by 4-NQO, enhance the expression of phosphorylated AKT, with subsequent phosphorylation of GSK3

OBJECTIVE: To investigate the maximum dose of continuous mivacurium infusion for intraoperative neuromonitoring (IONM) and observe the adverse reactions during thyroid surgery under total intravenous anesthesia (TIVA). METHODS: Thirty patients undergoing IONM during thyroid surgery received continuous infusion of mivacurium at the initial rate of 14.97 μg · kg RESULTS: The EC CONCLUSIONS In patients undergoing thyroid surgery under TIVA, the EC
Anesthesia, Intravenous ; Humans ; Mivacurium ; Propofol ; Remifentanil ; Thyroid Gland

Objective Toexploreasimpleandaccuratemethodfordeterminingthequantitativeevaluationandthevolumedegree divisionofpleuraleffusionbyMDCTtissuesegmentationtechnology.Methods Thevolumeofwaterinjectedintothreecontainersof differentshapeswasmeasuredby MDCTtissuesegmentationtechnique,andwerefurthercomparedwiththevolumeoftheactual waterinjection.Byusingthesamemethod,theliquidvolumeofpleuraleffusionbeforeandafterpumpingfrompatientswasmeasured andthedifferencebetweentheanteriorandtheposteriorwascalculated.Thenthedifferencebeforeandafterthepumpingwascomparedwiththe actualvolumeofclinicalfluidpumping.Atthesametime,thespecificmethodtodeterminethedegreedivisionofpleuraleffusionwas established.Results Forcontainersofdifferentshapes,theresultfromtheCTscanningliquidmeasurementwasthesameasactualwater injection,andhadahighcorrelation.Thedifferencebetweenbeforeandafterfluidextractionandclinicalactualfluidextractionwas linearcorrelated(positivecorrelation,r=0.986,P<0.001).Accordingtothemethodfordegreedivisionofadulthemothorax,MDCT tissuesegmentationtechnologydividedthevolumeofpleuraleffusionbeforethepumpingintosmall,mediumandlargeamounts.There were44caseswithsmallpleuraleffusion,11caseswithmoderatepleuraleffusionand7caseswithlargepleuraleffusion.Conclusion The volumeofpleuraleffusion measuredby MDCTtissuesegmentationtechnologycanreflecttheactualvolumeofpleuraleffusionin clinictoacertainextent,and MDCTtissuesegmentationtechnologycanbeusedtodividethevolumedegreeofpleuraleffusion.

Objective To investigate the effect of intracarotid administration of bone marrow stem cells (BMSCs) on learning and memory abilities of vascular dementia (VD) rats and the variability of expressions of neuron-specific enolase (NSE) and S-100 protein in the serum.Methods BMNCs collected from clean juvenile male Wistar rat femur were isolated; after adherent culture,the 3-4th generation BMSCs were performed induced differentiation.Ninety Wistar rats (age:18 to 24 months old) were randomly divided into three groups:control group,model group and treatment group,and each group was further classified into three subgroups by means of survival times of 2,4 and 8 weeks,which means that every subgroup consisted often rats.All VD rats,which were modeled by modified Pulsinellis 4-vessel occlusion (4VO); and those in the treatment group were injected 0.5 mL 1.2×107/mL BMSCs into the internal carotid artery 24 after operation.The learning and memory abilities of each rat in all subgroups were analyzed by shuttle box testing and the NSE leves and S-100 protein in serum were detected by enzyme-linked immuno sorbent assay (ELISA).Results The mean active avoidance response (AAR) ratios of the rats in the three subgroups (survival time of 2,4,8 weeks) of the model group,were,respectively,lower than those of the control group (P＜0.05),and those of the treatment group were significantly higher than those of the model group (P＜0.05).The serum levels of NSE in the treatment group was significantly lower than that in the model group two weeks after operation (P＜0.05),but it reversed 4 and 8 weeks after operation.The S-100 protein expressions in the treatment group was statistically lower than that in the model group (P＜0.05),however,there is no significant difference between treatment group and control group (P＞0.05).Conclusion Intracarotid administration of BMSCs obviously improves the learning and memory abilities of the VD rats,remarkably reduces the serum levels of NSE and S-100 protein,and efficiently relieves the injury degree of neurons and glial cells,which indicates that BMSCs therapy has effective protection on neural regeneration,neuronal proliferation and synaptic connectivity.