Objective:To identify key genes involved in LPS-induced acute lung injury(ALI)using GEO database,to eluci-date its pathogenesis and discover potential therapeutic drugs.Methods:Gene expression data from LPS-induced ALI was collected by GEO database,and microbiotics online analysis platform was employed to identify differential expression genes,from which core genes were obtained.Metascape and DAVID were used for GO and KEGG enrichment analysis of these core genes to identify pathways associated with ALI.GSEA and protein interaction analysis were performed for these pathways for identification of core targets.Poten-tial therapeutic drug,kaempferol-3-O-α-L-(4″-E-p-coumaroyl)-rhamnoside(KAE)was validated by animal experiments.Results:Two GEO datasets were incorporated and 261 core genes with differential expression were identified.Data visualization indicated that LPS-induced ALI predominantly involved inflammatory pathways,such as TNF and NF-κB.In vivo validation was conducted in mice on two core targets within this pathway:NF-κB and NLRP3,potential therapeutic drug KAE was administered,which was found to mitigate LPS-induced lung tissue damage.Further investigations demonstrated that KAE could effectively improve ALI by reducing expressions of p-NF-κB,NLRP3 and other proteins.Conclusion:This study markes screening of LPS-induced ALI model within GEO database.Above findings reveal predominant involvement of NF-κB and NLRP3 within TNF and NF-κB signaling pathways in LPS-in-duced ALI.KAE has potential for ALI treatment by down-regulating expressions of p-NF-κB,NLRP3 and other proteins,expecting to be a candidate drug for ALI treatment.

Objective:To identify key genes involved in LPS-induced acute lung injury(ALI)using GEO database,to eluci-date its pathogenesis and discover potential therapeutic drugs.Methods:Gene expression data from LPS-induced ALI was collected by GEO database,and microbiotics online analysis platform was employed to identify differential expression genes,from which core genes were obtained.Metascape and DAVID were used for GO and KEGG enrichment analysis of these core genes to identify pathways associated with ALI.GSEA and protein interaction analysis were performed for these pathways for identification of core targets.Poten-tial therapeutic drug,kaempferol-3-O-α-L-(4″-E-p-coumaroyl)-rhamnoside(KAE)was validated by animal experiments.Results:Two GEO datasets were incorporated and 261 core genes with differential expression were identified.Data visualization indicated that LPS-induced ALI predominantly involved inflammatory pathways,such as TNF and NF-κB.In vivo validation was conducted in mice on two core targets within this pathway:NF-κB and NLRP3,potential therapeutic drug KAE was administered,which was found to mitigate LPS-induced lung tissue damage.Further investigations demonstrated that KAE could effectively improve ALI by reducing expressions of p-NF-κB,NLRP3 and other proteins.Conclusion:This study markes screening of LPS-induced ALI model within GEO database.Above findings reveal predominant involvement of NF-κB and NLRP3 within TNF and NF-κB signaling pathways in LPS-in-duced ALI.KAE has potential for ALI treatment by down-regulating expressions of p-NF-κB,NLRP3 and other proteins,expecting to be a candidate drug for ALI treatment.

The protein production system using a baculovirus Antheraea pernyi nucleopolyhedrovirus (AnpeNPV) as a gene expression vector and its host insect as a natural bioreactor was successful established and its excellent performance in the protein production has been demonstrated. In this paper, the system is used to produce recombinant human epidermal growth factor (rhEGF), which have been widely used in medical and cosmetic treatment. A recombinant AnpehEGF virus has been constructed by replacing the viral polyhedrin gene with the rhEGF gene, and then injected it to Samia cynthia ricini pupae. Amplification and expression of rhEGF gene in the pupae was clearly detected by PCR, Western blot and ELISA analyses. These analyses have also revealed that rhEGF in the pupae was significantly increased at 6 days post-infection, and reached maximum level at the 12th day. The concentrations of rhEGF were 19.77, 24.90, 618.59 and 1 952.46 ng/g pupae at 3, 6, 9 and 12 days post-infection, respectively. However, the rhEGF concentration reduced at later stage (days 15). The rhEGF in the pupae could be purified using ammonium sulfate precipitation and Ni-NTA agrose affinity chromatography. Results demonstrate that Samia cynthia ricini pupae can be used as a bioreactor to produce rhEGF and, if successfully improved, will be a novel method of rhEGF production with lower cost and more efficient.
Amino Acid Sequence ; Animals ; Bombyx ; genetics ; metabolism ; Epidermal Growth Factor ; biosynthesis ; genetics ; Genetic Vectors ; genetics ; Humans ; Molecular Sequence Data ; Nucleopolyhedrovirus ; genetics ; Recombinant Proteins ; biosynthesis ; genetics

Objective To evaluate the best mean for the treatment of varicocele. Methods The clini-cal effects of the high ligation of spermatic vein under the small incisal opening of the poritneum meta abdom-inal membrane and the ligation of spermatic vein under the laparoscope for the varicocele were retrospectively reviewed. The operating time, the postoperative days and the cost of hospitalization were compared of the two methods. Results Comparing with the group of operation and the group of laparoscope. Among the 158 vat-icocele patients with unilateral or bilateral varicocele, there was not statistical difference in the operating time between the two methods (P > 0. 05), whi]e there was significant difference in the postoperative days and the cost of hospitalization(P <0. 05). Conclusions When the bilateral varicoceles were simultaneously ligated, the high ligation of spermatic vein under the laparoscope was the best selection; if the unilateral varicocele was highly ligated or the patients were unconditional or the patients were not fit for laparoscopic operation, the high ligation of spermatic vein under the small incisal opening of peritoneum was the best operating method.