Objectives:To provide a new perspective on the epidemiological characteristics of vertebral column fracture in China and to guide the formulation of targeted public health strategies.Methods:The Global Burden of Disease 2021 database was utilized to analyze the epidemiological trends of vertebral column fracture in China from 1990 to 2021.The study further explored the disease burden characteristics related to age,sex,and etiology.The Joinpoint regression model was employed to calculate the average annual percent change(AAPC)to analyze the temporal trends of age-standardized rates.The Bayesian age-period-cohort(BAPC)model was used to forecast the age-standardized rates for 2035.Results:In 2021,the incidence and prevalence of vertebral column fracture in China were 1194465[95％uncertainty interval(UI):888994-1594120]and 717078(95％UI:619114-835347),respectively,representing increases of 52.28％and 113.66％compared to 1990.From 1990 to 2021,the age-standardized incidence rate(ASIR)in China showed no significant upward trend,with an AAPC of 0.45％[95％confidence interval(CI):-0.29％to 1.20％,P＞0.05],while the age-standardized prevalence rate(ASPR)showed a significant upward trend,with an AAPC of 0.53％(95％CI:0.24％to 0.83％,P＜0.05).The BAPC prediction results for 2022-2035 indicated a downward trend for both ASIR and ASPR.Analysis by age group revealed that the peak age groups for incidence and preva-lence have increased,with the crude incidence rate(CIR)and crude prevalence rate(CPR)increasing with age,reflecting an aging trend.Gender analysis found that the disease burden was more prominent among middle-aged men and elderly women.Etiological analysis revealed that falls were the leading cause of spinal frac-tures in China,followed by road injuries.Conclusions:From 1990 to 2021,the disease burden of spinal fractures in China has significantly increased and shown an aging trend.Although the predicted disease bur-den for 2035 is expected to decline,it is still necessary to remain vigilant.Middle-aged men and elderly women are the key populations of concern,and emphasis should be placed on preventing falls and road in-juries.

Objectives:To provide a new perspective on the epidemiological characteristics of vertebral column fracture in China and to guide the formulation of targeted public health strategies.Methods:The Global Burden of Disease 2021 database was utilized to analyze the epidemiological trends of vertebral column fracture in China from 1990 to 2021.The study further explored the disease burden characteristics related to age,sex,and etiology.The Joinpoint regression model was employed to calculate the average annual percent change(AAPC)to analyze the temporal trends of age-standardized rates.The Bayesian age-period-cohort(BAPC)model was used to forecast the age-standardized rates for 2035.Results:In 2021,the incidence and prevalence of vertebral column fracture in China were 1194465[95％uncertainty interval(UI):888994-1594120]and 717078(95％UI:619114-835347),respectively,representing increases of 52.28％and 113.66％compared to 1990.From 1990 to 2021,the age-standardized incidence rate(ASIR)in China showed no significant upward trend,with an AAPC of 0.45％[95％confidence interval(CI):-0.29％to 1.20％,P＞0.05],while the age-standardized prevalence rate(ASPR)showed a significant upward trend,with an AAPC of 0.53％(95％CI:0.24％to 0.83％,P＜0.05).The BAPC prediction results for 2022-2035 indicated a downward trend for both ASIR and ASPR.Analysis by age group revealed that the peak age groups for incidence and preva-lence have increased,with the crude incidence rate(CIR)and crude prevalence rate(CPR)increasing with age,reflecting an aging trend.Gender analysis found that the disease burden was more prominent among middle-aged men and elderly women.Etiological analysis revealed that falls were the leading cause of spinal frac-tures in China,followed by road injuries.Conclusions:From 1990 to 2021,the disease burden of spinal fractures in China has significantly increased and shown an aging trend.Although the predicted disease bur-den for 2035 is expected to decline,it is still necessary to remain vigilant.Middle-aged men and elderly women are the key populations of concern,and emphasis should be placed on preventing falls and road in-juries.

Animals ; Antigens, Surface/genetics* ; Cell Communication/genetics* ; Copulation/physiology* ; Fallopian Tubes/metabolism* ; Female ; Fertilization/genetics* ; GPI-Linked Proteins/genetics* ; Gene Expression Regulation ; Genes, Reporter ; Green Fluorescent Proteins/metabolism* ; Litter Size ; Luminescent Proteins/metabolism* ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Mitochondria/metabolism* ; Reproduction/genetics* ; Signal Transduction ; Sperm Count ; Sperm Motility/genetics* ; Spermatozoa/metabolism* ; Uterus/metabolism*

Objective To investigate the effect of PTEN gene in the mouse uterus during embryo implantation.Methods Real-time fluorescence quantitative PCR(FQ-PCR) was used to detect PTEN mRNA expressed in the endometria of the nonpregnant mice and the late pregnant mice(day 1,day 3,day 4,day 5 and day 7),with 20 mice sacrificed at each fixed day.Out of another 20 3-day pregnant mice,ten received PTEN antisense oligonucleotide at the horn of uterus and ten received normal saline to count the blastocysts at pregnant day 8.Results The PTEN mRNA/?-actin mRNA in pregnant mice was higher than that of nonpregnant mice,gradually hoisted as days passed by,and reached the highest at pregnant day 5.The number of blastocysts in the mice that received PTEN antisense oligonucleotide was fewer than that received normal saline.Conclusion PTEN persistently expresses in mouse endometria during the early pregnancy and maybe participate in the regulation process of mouse blastodyst implantation.