OBJECTIVETo obtain geranylgeranyl diphosphate synthase gene of Salvia miltiorrhiza, and conduct bioinformatic and transcript expression analysis of the cloned SmGGPS1 gene.

METHODThe degenerate primers were designed based on the conservative regions of GGPS protein sequences from public databases. The target gene was obtained from root of S. miltiorrhiza by use of homologous cDNA amplification and RACE technologies. The sequence alignment was performed using BLAST. The open reading frame was identified by use of the ORF Finder. The protein domains were defined by use of Prosite software and the signal peptide sequence was predicted by Target P1.1. MEGA4.0 was used to conduct multiple amino acid sequence alignment and construct the phylogenetic tree. Roots and leaves at the seedlings stage and roots, stems, leaves, buds and flowers in the flowering stage were sampled for transcript analysis. Semi-quantitative RT-PCR was used to detect the gene expression level. The complete gene of GGPS was obtained from S. miltiorrhiza genomic DNA by PCR using the cDNA-derived specific primer. The gene structure of GGPS was analyzed by comparison of the genomic DNA and its cDNA.

RESULTThe obtained 1 298 bp SmGGPS1 cDNA sequence contains an 1095 bp ORF, encoding 364 amino acids. It is predicted that it has a plastid targeting signal peptide of approximately 52 amino acid at the N-terminal end. It is to believe that this is the polyprenyl synthetase signature, and nucleic acid sequence comparison revealed that SmGGPS1 ORF has more than 60% identity to the reported GGPS. RT-PCR semi-quantitative analysis showed that the gene expresses in the all tested tissues, and with much higher level of expression in the leaves in the flowering stage. SmGGPS1 has a 397 bp intron.

CONCLUSIONFor the first time the cloning of geranylgeranyl diphosphate synthase gene from S. miltiorrhiza was reported, and it provides a good basis for further functional study of SmGGPS1.

Amino Acid Sequence ; Base Sequence ; Cloning, Molecular ; Farnesyltranstransferase ; chemistry ; genetics ; metabolism ; Gene Expression Regulation, Enzymologic ; Molecular Sequence Data ; Open Reading Frames ; Phylogeny ; Plant Proteins ; genetics ; metabolism ; Plants ; classification ; enzymology ; genetics ; Salvia miltiorrhiza ; classification ; enzymology ; genetics

Objective: To observe the effects of nutrients-compound supplementation (NS) on cognitive function. Method: Forty six young male volunteers were randomly divided into 2 groups, NS and control (CT). NS group was supplemented with nutrients-compound while CT group received placebo for 60 d.The cognitive function of all volunteers were examined by 《CHANG-AN Handbook of Group Intelligence Test》 and Wechsler Memory Scale(WMS). Their vitamin status was assessed by 4-h load test at the end of experiment. Results : The vitamin nutriture of NS group was significantly better than that of CT. Many achievements of cognitive test of NS group were obviously increased compared to that before supplementation or that of CT. The visual responding time was shortened in 3 tests and the error number was decreased in 1 test of NS group, while no significant change was observed in CT group. Conclusion: Supplementation with the nutrients-compound is beneficial to improvement of cognitive function such as learning, memory and understanding.

Objective: To investigate the dietary nutritional status of the intellectuals in three cities in China. Methods: The intellectuals aged over 45, with senior academic title, working in universities or research institutes in Tianjin, Guangzhou and Chengdu, were selected as volunteers. Food frequency questionair was used to conduct the diretary survey. And the results were compared between the intellectuals of nutrition and non-nutrition speciality. Results: Compared with Dietary Guideline and Balance Diet Pagoda for Chinese Residents, the intakes of cereals, milk, fruits,and egg of the vulunteers were adequate, but the intakes of meat and oils were more, while those of fish and shrimp, legume,and vegetables were less than the suggested levels in Pagoda. The food pattern of the intellectuals in nutrition speciality was more advantageous than that in non-nutrition. Conclusion: The dietary pattern of the intellectuals should be improved, according to the Dietary Guideline and Balanced Diet Pagoda for Chinese Residents.

Objective To teach the intellectuals how to adapt psychological stress and improve health by rational nutrition and health education. Method The propaganda and education on the role of nutrients in increasing the adaptive ability to psychological stress and ameliorating related diseases were used, mainly in the way of seminar and consultation, and the psychological status was evaluated by testing A-type behavior, anxiety and depression, and determining the concentration of saliva cortisol, serum homocysteine and lipid peroxides. Results After education, A type behavior, SDS total scores and the concentration of saliva cortisol, serum homocysteine and lipid peroxides were decreased dramatically, indicating that the psychological stress of intellectuals was effectively reliveved. Conclusion Nutritional propaganda and education can significantly improve intellectuals’ psychological health status.

The function of Medical Engineering profession in military hospitals becomes more and more important,so does the development of the professional person troops.There is an urgent need of a professional person troop with high-tech professional knowledge,rigorous science style and good managerial ability.Therefore,it is necessary to develop the professional person troops in Medical Engineering in military hospital.

0.05). Conclusion After heated, the physical stability of UAE and UAS is reduced, the viscosity become lower, ADM releasing rate is fell. The heated Lipiodol-Adriamycin pharmaceutics had advantage in the interventional embolization chemotherapy of the neoplasm.

AIM: To investigate the effect of 17? estradiol (17?E 2)on atherosclerosis and hemorrheology in ovariectomized (OVX) rabbits. METHODS: Thirty four female rabbits were divided into 4 groups, group A: normal control; group B: sham+CHO; group C: OVX+CHO; group D: OVX+CHO+17? E 2. Cholesterol(CHO) was fed to rabbits for 12 weeks, before and after feeding CHO, the serum lipid (TC, TG, HDL-C, LDL-C, ApoA1, ApoB) and area of aortic atherosclerotic plaques were measured. Blood viscosity, plasma viscosity (?p), aggregation index of RBC (AIRC) and fibrinogen were also determined, respectively. RESULTS: ① The ratio of area of aortic atherosclerotic plaque to total area of aortic intima was 0.02?0.003 (in group A), 0.42?0.15 (group B), 0.67?0.23 (group C), and 0.12?0 11 (group D), respectively. In group D, the ratio of aortic atherosclerotic plaque was markedly decreased compared with group C ( P

Objective To examine the possibility that homocysteine(Hcy) may augment ?-amyloid(A?) neurotoxicity.Method Cultured hippocampal neurons were treatd with Hcy and/or A?42 and it's apoptosis,calcium influx,DNA damage and oxidative injury were examined.Results Combined treatment with 250?mol/L Hcy and 10?mol/L A?42 increased the apoptosis rate of hippocampal neurons significantly more than either agent alone,and even the sum of each agent treated alone.The combined treatment significantly exceeded the cytosolic calcium accumulation and MDA obtained with either alone or the sum of each agent.A significant increase in DNA damage also occurred in neurons exposed to A?42 in the presence of Hcy,but the magnitude of the increase was not greater than that seen with either treatment alone.Conclusion Hcy could potentiate A? neurotoxicity by neuron exitotoxicity,oxidative stress and inducing apoptosis.

Objective:To study the effects of zinc deficiency on bone histomorphometric parameters of femoral distal diaphysis in rats. Methods:Thirty Wistar rats were randomly allocated into three groups:the zinc-deficient group(ZD), the control group(Cont),and the pair-fed group(ZP). After the rats had been fed for eight weeks, the histomorphometric and dynamic parameters of the rats were analysed using bone histomorphometric method. Results:The number, volume and connectivity of trabecular bone, and the mean trabecular plate density of ZD rats were significantly decreased, but their mean trabecular plate space was significantly increased. In addition, the data showed that ZD animals had significantly decreased trabecular osteoid surface, reduced velocity of bone formation as compared with Cont and ZP animals. The results showed that in ZD rats the mineral deposit rate was significantly slow, while the mineralization lag and osteoid maturation period were obviously prolonged. Conclusion: Zinc deficiency reduces velocity of bone formation and prolongs bone mineralization and destroys bone structure.

Objective: To study the effects of different niacin nutritures on behaviors of stressed rats. Methods: 100 Wistar male rats were assigned into 10 groups:control, pair-fed, nicotinamide (NAM) deficiency (ND), low-dose NAM supplement (LNS 40 mg/kg diet) and high-dose NAM supplement (HNS 500 mg/kg diet), and the corresponding restrained groups. 24 h urinary excretion of N1-methylnicotinamide (NMN), body weight changes, behaviors in the open-field test, plasma cortisol, nitric oxide(NO) and liver nitric oxide synthase(NOS) were determined. Results: Compared with the control group, restrained rats had relatively lower urinary NMN. Both ND and restrainted stress retarded the body weight gains . Rats in the control and LNS groups had relatively shorter latency time in the open-field test. Restrained rats had higher plasma cortisol and NO than those in the corresponding control group. However, rats with HNS had the lowest plasma cortisol concentrations. Dietary supplement of NAM increased liver NOS . Conclusions: Restraint stress may increase niacin depletion and appropriate NAM supplement has positive effects on stress adaptation in rats.