Objective:To investigate disease characteristics of patients with psoriatic arthritis (PsA) based on the PsA cohort in West China Hospital, so as to provide a reference for clinicians in its diagnosis, treatment, and evaluation strategy formulation.Methods:A cross-sectional study was carried out, and a descriptive analysis was conducted on clinical data from PsA patients who were treated at the Department of Dermatology, West China Hospital, Sichuan University between April 2, 2020, and January 21, 2025. Demographic characteristics, clinical manifestations, laboratory and imaging findings, and treatment modalities were analyzed.Results:A total of 530 PsA patients were included, of whom 332 (62.6%) were males and 198 (37.4%) were females, with ages of 44.1 ± 12.4 years. Skin lesions preceded joint symptoms in 452 patients (85.3%), with time intervals ( M [ Q1, Q3]) of 8.0 (3.0, 15.0) years. Overweight or obesity was observed in 319 patients (60.2%), and 188 (35.5%) had comorbid fatty liver. Peripheral joint involvement was common (485 cases, 91.5%), with the proximal interphalangeal joints being most frequently affected by tenderness (172 cases, 35.5%) and swelling (119 cases, 24.5%) ; the number of enthesitis cases identified by ultrasonography (116 cases, 23.9%) was significantly higher than that by clinical examination (82 cases, 15.5%) ; axial joint involvement was observed in 258 patients (48.7%), with the sacroiliac joints most commonly affected (201 cases, 77.9%). Regarding treatment, conventional systemic drugs were predominant in the treatment of psoriasis prior to the diagnosis of PsA; after the diagnosis of PsA, the number of patients receiving targeted therapies increased to 334 (63.0%), with interleukin-17 inhibitors being the most common (140 cases, 26.4%), followed by tumor necrosis factor-α inhibitors (106 cases, 20.0%) and Janus kinase inhibitors (39 cases, 7.4%) . Conclusions:PsA predominantly affects males over 40 years old and is characterized by preceding skin lesions, delayed diagnosis, and multiple comorbidities. High-frequency ultrasound has advantages in the early detection of peripheral enthesitis. Further attention is needed for managing comorbidities such as fatty liver and obesity-related metabolic conditions.

Fatty liver is common disease of nutritional metabolism in the perinatal period,character-ized by elevated levels of NEFA in the blood and disorders of lipid metabolism.High concentra-tions of NEFA damage mitochondria and promote the release of reactive oxygen species(ROS).At the same time,lipid peroxidation occurs in lipid accumulation in hepatocytes,producing free radi-cals such as ROS,which leads to oxidative stress in the liver.When the level of intracellular ROS increases,thioredoxin-interacting protein(TXNIP)activates nucleotide-binding oligomerization structure-like protein 3(NLRP3)inflammasomes,and oxidative stress can regulate pyroptosis,but it is unclear whether reactive oxygen species(ROS)produced by oxidative stress in hepatocytes can mediate pyroptosis and induce liver injury in dairy cows through the TXNIP/NLRP3 pathway.In this study,liver tissue samples from healthy dairy cows and fatty liver cows were collected,and NEFA was used to construct a fatty liver cell model,and triglyceride(TG)content and oxidative stress related indicators were detected by kit.Western blot was used to detect the activation of NL-RP3 inflammasomes,the inflammatory pathway of NF-κB and the expression levels of pyroptosis-related proteins.Fluorescence quantitative PCR was used to detect the relative expression level of inflammatory factor mRNA.The results showed that compared with the healthy(control)group,the TG content of fatty liver tissue and fatty liver cells was significantly increased(P＜0.05),the activities of SOD and GSH-Px were significantly decreased(P＜0.05),and the protein expression levels of TXNIP,NLRP3,GSDMD-N and Caspase-1 were significantly up-regulated(P＜0.05).The results of the antioxidant model of fatty hepatocytes using NEFA and antioxidants(NAC)showed that compared with the fatty hepatocyte model,the content of ROS in hepatocytes was sig-nificantly reduced,and oxidative stress,NLRP3 inflammasome activation and pyroptosis were alle-viated.In summary,this study found that when fatty liver disease occurs in dairy cows,ROS pro-duced by oxidative stress in the liver can mediate pyroptosis through the TXNIP/NLRP3 path-way,which can lead to liver injury in fatty liver cows.

Fatty liver is common disease of nutritional metabolism in the perinatal period,character-ized by elevated levels of NEFA in the blood and disorders of lipid metabolism.High concentra-tions of NEFA damage mitochondria and promote the release of reactive oxygen species(ROS).At the same time,lipid peroxidation occurs in lipid accumulation in hepatocytes,producing free radi-cals such as ROS,which leads to oxidative stress in the liver.When the level of intracellular ROS increases,thioredoxin-interacting protein(TXNIP)activates nucleotide-binding oligomerization structure-like protein 3(NLRP3)inflammasomes,and oxidative stress can regulate pyroptosis,but it is unclear whether reactive oxygen species(ROS)produced by oxidative stress in hepatocytes can mediate pyroptosis and induce liver injury in dairy cows through the TXNIP/NLRP3 pathway.In this study,liver tissue samples from healthy dairy cows and fatty liver cows were collected,and NEFA was used to construct a fatty liver cell model,and triglyceride(TG)content and oxidative stress related indicators were detected by kit.Western blot was used to detect the activation of NL-RP3 inflammasomes,the inflammatory pathway of NF-κB and the expression levels of pyroptosis-related proteins.Fluorescence quantitative PCR was used to detect the relative expression level of inflammatory factor mRNA.The results showed that compared with the healthy(control)group,the TG content of fatty liver tissue and fatty liver cells was significantly increased(P＜0.05),the activities of SOD and GSH-Px were significantly decreased(P＜0.05),and the protein expression levels of TXNIP,NLRP3,GSDMD-N and Caspase-1 were significantly up-regulated(P＜0.05).The results of the antioxidant model of fatty hepatocytes using NEFA and antioxidants(NAC)showed that compared with the fatty hepatocyte model,the content of ROS in hepatocytes was sig-nificantly reduced,and oxidative stress,NLRP3 inflammasome activation and pyroptosis were alle-viated.In summary,this study found that when fatty liver disease occurs in dairy cows,ROS pro-duced by oxidative stress in the liver can mediate pyroptosis through the TXNIP/NLRP3 path-way,which can lead to liver injury in fatty liver cows.

Objective:To investigate disease characteristics of patients with psoriatic arthritis (PsA) based on the PsA cohort in West China Hospital, so as to provide a reference for clinicians in its diagnosis, treatment, and evaluation strategy formulation.Methods:A cross-sectional study was carried out, and a descriptive analysis was conducted on clinical data from PsA patients who were treated at the Department of Dermatology, West China Hospital, Sichuan University between April 2, 2020, and January 21, 2025. Demographic characteristics, clinical manifestations, laboratory and imaging findings, and treatment modalities were analyzed.Results:A total of 530 PsA patients were included, of whom 332 (62.6%) were males and 198 (37.4%) were females, with ages of 44.1 ± 12.4 years. Skin lesions preceded joint symptoms in 452 patients (85.3%), with time intervals ( M [ Q1, Q3]) of 8.0 (3.0, 15.0) years. Overweight or obesity was observed in 319 patients (60.2%), and 188 (35.5%) had comorbid fatty liver. Peripheral joint involvement was common (485 cases, 91.5%), with the proximal interphalangeal joints being most frequently affected by tenderness (172 cases, 35.5%) and swelling (119 cases, 24.5%) ; the number of enthesitis cases identified by ultrasonography (116 cases, 23.9%) was significantly higher than that by clinical examination (82 cases, 15.5%) ; axial joint involvement was observed in 258 patients (48.7%), with the sacroiliac joints most commonly affected (201 cases, 77.9%). Regarding treatment, conventional systemic drugs were predominant in the treatment of psoriasis prior to the diagnosis of PsA; after the diagnosis of PsA, the number of patients receiving targeted therapies increased to 334 (63.0%), with interleukin-17 inhibitors being the most common (140 cases, 26.4%), followed by tumor necrosis factor-α inhibitors (106 cases, 20.0%) and Janus kinase inhibitors (39 cases, 7.4%) . Conclusions:PsA predominantly affects males over 40 years old and is characterized by preceding skin lesions, delayed diagnosis, and multiple comorbidities. High-frequency ultrasound has advantages in the early detection of peripheral enthesitis. Further attention is needed for managing comorbidities such as fatty liver and obesity-related metabolic conditions.

Objective:To explore the effects of hyperbaric oxygen treatment on the serum chemokines and immune T cells in the patients with bronchial asthma, and to analyze the correlation between the treatment and the activity of cytochrome oxidase (COX).Methods:A total of 96 patients with bronchial asthma treated from January 2017 to December 2018 were selected as the research subjects, and were randomly divided into control group and observation group ( n=48). The control group was given the conventional treatment, while the observation group was additionally treated with the hyperbaric oxygen therapy. The changes of serum chemokines and the changes of Th1 and Th2 (subsets of CD 4+ T cells) in the two groups were compared before and after the treatment. The correlation between the hyperbaric oxygen therapy and the COX activity was also analyzed. Results:In the observation group, 38 cases were markedly improved, while only 27 cases in the control group were markedly improved. And the total effective rate of the observation group (95.83%) was significantly higher than that of the control group (85.42%) ( P<0.05). After the treatment, the partial pressure of oxygen (PaO 2), FEV1, FEV1/FVC of the patients in the observation group were significantly increased as compared with those in the control group with statistically significant differences ( P<0.05). The serum eotaxin and monocyte chemoattractant protein-1 (MCP-1) in both two groups after treatment were significantly decreased as compared with those before the treatment, and the serum chemokines of the observation group were sharply reduced compared with that of the control group ( P<0.05). After the treatment, the Th1 in the peripheral blood and the ratio of Th1/Th2 of patients in the two groups increased significantly, while the Th2 decreased significantly. Moreover, the distribution of immune T cell subgroups in the observation group was better than that in the control group. According to person correlation analysis, the levels of eotaxin, MCP-1, and Th2 were negatively correlated with COX activity ( r=-0.635, P=0.004; r=-0.673, P=0.002; r=-0.737, P<0.001), while the Th1 was positively correlated with COX activity ( r=0.684, P=0.001). Conclusion:Hyperbaric oxygen therapy can strengthen the immune function of the patients with bronchial asthma and improve the prognosis by enhancing COX activity and active aerobic metabolism, thus reducing the expression of serum chemokines.

AIM: To investigate the role of ribonucleotide reductase M2 (RRM2) in 5-fluorouracil (5-FU) resistance of HCC cells and to develop potential strategies to enhance the sensitivity of HCC cells to 5-FU. METHODS: The expression of RRM2 was examined by Western blot in BEL/5-FU cells and BEL7402 cells. The expression of RRM2 was down-regulated through RNA interference (RNAi) technology and the activity of RRM2 was inhibited by the RRM2 inhibitor 3-AP. The cell proliferation ability was detected by CCK-8 assay and colony formation assay, and the apoptosis was analyzed by Confocal high-content system. RESULTS: The expression level of RRM2 was increased by 2.5-fold in BEL/5-FU cells compared with BEL7402 cells. Compared with control siRNA, the half maximum inhibitory concentration IC

Objective:To explore the effects of hyperbaric oxygen treatment on the serum chemokines and immune T cells in the patients with bronchial asthma, and to analyze the correlation between the treatment and the activity of cytochrome oxidase (COX).Methods:A total of 96 patients with bronchial asthma treated from January 2017 to December 2018 were selected as the research subjects, and were randomly divided into control group and observation group ( n=48). The control group was given the conventional treatment, while the observation group was additionally treated with the hyperbaric oxygen therapy. The changes of serum chemokines and the changes of Th1 and Th2 (subsets of CD 4+ T cells) in the two groups were compared before and after the treatment. The correlation between the hyperbaric oxygen therapy and the COX activity was also analyzed. Results:In the observation group, 38 cases were markedly improved, while only 27 cases in the control group were markedly improved. And the total effective rate of the observation group (95.83%) was significantly higher than that of the control group (85.42%) ( P<0.05). After the treatment, the partial pressure of oxygen (PaO 2), FEV1, FEV1/FVC of the patients in the observation group were significantly increased as compared with those in the control group with statistically significant differences ( P<0.05). The serum eotaxin and monocyte chemoattractant protein-1 (MCP-1) in both two groups after treatment were significantly decreased as compared with those before the treatment, and the serum chemokines of the observation group were sharply reduced compared with that of the control group ( P<0.05). After the treatment, the Th1 in the peripheral blood and the ratio of Th1/Th2 of patients in the two groups increased significantly, while the Th2 decreased significantly. Moreover, the distribution of immune T cell subgroups in the observation group was better than that in the control group. According to person correlation analysis, the levels of eotaxin, MCP-1, and Th2 were negatively correlated with COX activity ( r=-0.635, P=0.004; r=-0.673, P=0.002; r=-0.737, P<0.001), while the Th1 was positively correlated with COX activity ( r=0.684, P=0.001). Conclusion:Hyperbaric oxygen therapy can strengthen the immune function of the patients with bronchial asthma and improve the prognosis by enhancing COX activity and active aerobic metabolism, thus reducing the expression of serum chemokines.

The immune microenvironment plays an important role in the occurrence and development of breast cancer. The infiltrating immune cells and the produced inflammatory cytokines in the tumor microenvironment regulate the growth, proliferation and metastasis of breast cancer. In this article, the roles and related mechanisms of nonspecific immune microenvironment in breast cancer are summarized, focusing on the natural killer cells, dendritic cells, myeloid derived suppressor cells, tumor associated macrophages, interleukins, chemokines, tumor necrosis factor-α, transforming growth factor-β and so on.
Breast Neoplasms ; immunology ; physiopathology ; Chemokines ; immunology ; Dendritic Cells ; immunology ; Humans ; Macrophages ; immunology ; Research ; trends ; Tumor Microenvironment ; immunology

Objective To understand the risk of acute renal injury (AKI)in patients using compound diclofenac sodium injection after surgeries and analyze the influencing factors. Methods Data of patients who had normal renal function before receiving compound diclofenac sodium injection treatment after surgeries in 2015 in the Second Xiangya Hospital of Central South University were collected and studied retrospectively (unmatched case-control study). The patients with AKI after treatment were included in the case group and the patients without AKI were included in the control group. The general condition, postoperative medication,and renal function before and after medication in the 2 groups were compared. The risk and related factors of AKI using compound diclofenac sodium injection were analyzed. Results A total of 821 patients were enrolled into this study,including 63 cases in the case group [43 males and 20 females,average age(51 ± 13)years]and 758 cases in the control group [425 males and 33 females, average age(50 ±14)years]. The proportion of patients with hypertension and liver cirrhosis in the case group was higher than that in the control group [25. 4％ (16/ 63)vs. 13. 1％ (99/ 758),P =0. 009;9. 5％(6 / 63) vs. 2. 8％ (21 / 758),P = 0. 013],the proportion of patients with general surgeries in the case group was higher than that in the control group [42. 9％(27 / 63)vs. 26. 9％ (204 / 758),P = 0. 007],the proportion of patients with neurosurgery in the case group was lower than that in the control group [15. 9％(10 / 63)vs. 33. 5％ (254 / 758),P = 0. 004],the proportion of patients using compound sodium diclofenac injection within 24 h after operation in the case group was higher than that in the control group [20. 6％(13 / 63)vs.10. 7％(81 / 758),P = 0. 017]. The results of binary logistic regression analysis showed that the risk of AKI in patients with hypertension was significantly higher than the other patients with other diseases (OR =2. 847,95％ CI:1. 498-5. 410,P = 0. 001);the risk of AKI in patients with compound diclofenac sodium injection treatment within 24 h after operation was significantly higher than that in patients with compound diclofenac sodium injection treatment 24 h after the surgery(OR =1. 956,95％ CI:1. 154-3. 315,P =0. 013). Conclusion The patients with compound diclofenac sodium injection treatment after surgeries could develop AKI,the combination of hypertension and the use of the drug within 24 hours postoperatively could significantly increase the risk of AKI.

Objective To understand the risk of acute renal injury (AKI)in patients using compound diclofenac sodium injection after surgeries and analyze the influencing factors. Methods Data of patients who had normal renal function before receiving compound diclofenac sodium injection treatment after surgeries in 2015 in the Second Xiangya Hospital of Central South University were collected and studied retrospectively (unmatched case-control study). The patients with AKI after treatment were included in the case group and the patients without AKI were included in the control group. The general condition, postoperative medication,and renal function before and after medication in the 2 groups were compared. The risk and related factors of AKI using compound diclofenac sodium injection were analyzed. Results A total of 821 patients were enrolled into this study,including 63 cases in the case group [43 males and 20 females,average age(51 ± 13)years]and 758 cases in the control group [425 males and 33 females, average age(50 ±14)years]. The proportion of patients with hypertension and liver cirrhosis in the case group was higher than that in the control group [25. 4％ (16/ 63)vs. 13. 1％ (99/ 758),P =0. 009;9. 5％(6 / 63) vs. 2. 8％ (21 / 758),P = 0. 013],the proportion of patients with general surgeries in the case group was higher than that in the control group [42. 9％(27 / 63)vs. 26. 9％ (204 / 758),P = 0. 007],the proportion of patients with neurosurgery in the case group was lower than that in the control group [15. 9％(10 / 63)vs. 33. 5％ (254 / 758),P = 0. 004],the proportion of patients using compound sodium diclofenac injection within 24 h after operation in the case group was higher than that in the control group [20. 6％(13 / 63)vs.10. 7％(81 / 758),P = 0. 017]. The results of binary logistic regression analysis showed that the risk of AKI in patients with hypertension was significantly higher than the other patients with other diseases (OR =2. 847,95％ CI:1. 498-5. 410,P = 0. 001);the risk of AKI in patients with compound diclofenac sodium injection treatment within 24 h after operation was significantly higher than that in patients with compound diclofenac sodium injection treatment 24 h after the surgery(OR =1. 956,95％ CI:1. 154-3. 315,P =0. 013). Conclusion The patients with compound diclofenac sodium injection treatment after surgeries could develop AKI,the combination of hypertension and the use of the drug within 24 hours postoperatively could significantly increase the risk of AKI.