Objective:To explore the effect of the combination of robot-assisted total knee arthroplasty and pressure sensor on early postoperative outcomes.Methods:Twenty patients who underwent YUANHUA robot-assisted TKA between September 2024 and December 2024 were prospectively enrolled. After randomization and exclusion of one patient lost to follow-up, 10 patients were included in the pressure-sensor group and 10 in the control (no-pressure) group. In the pressure-sensor group, surgeons used a pressure sensor to assist in soft tissue balancing after osteotomy, whereas in the control group, balancing was performed empirically. In both groups, medial and lateral compartment pressures of the knee at 10°, 45°, 90°, and 120° of flexion were recorded using a pressure sensor prior to component implantation. Clinical outcomes were evaluated preoperatively and at 1 day, 3 days, 1 week, 2 weeks, 6 weeks, and 3 months postoperatively using the visual analog scale (VAS), Knee Society Score (KSS), Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), and Forgotten Joint Score (FJS).Results:All 20 procedures were completed successfully, and patients were followed for at least 3 months. At 10° of knee flexion, the difference between medial and lateral compartment pressures was significantly smaller in the pressure-sensor group (0.99±59.44 N) than in the control group (97.18±84.34 N; t=-2.948, P=0.009). At 45° of flexion, the corresponding differences were -12.99±36.20 N and 51.48±76.40 N, respectively ( t=-2.411, P=0.032). No significant differences in VAS, KSS, or WOMAC scores were observed between groups preoperatively ( P>0.05). At 3 months postoperatively, the KSS was significantly higher in the pressure-sensor group (174.40±16.39) compared with the control group (138.50±38.35, t=2.722, P=0.014). The WOMAC and VAS scores were significantly lower in the pressure-sensor group (12.70±11.00 and 1.20±0.92, respectively) than in the control group (27.30±18.54 and 2.70±2.00; t=-2.142, P=0.046; t=-2.153, P=0.045). At 2 weeks and 3 months postoperatively, the FJS scores in the pressure-sensor group [70.00±26.06 and 88.07(83.52, 95.83)] were significantly higher than those in the control group [37.92±32.42 and 50.00(32.50, 67.75); t=2.439, P=0.025; Z=-2.466, P=0.014]. Conclusion:The combination of precise osteotomy using robot-assisted TKA and soft tissue balancing guided by a pressure sensor provided more accurate medial-lateral compartment balance and significantly enhanced early postoperative clinical outcomes.

Objective:To explore the effect of the combination of robot-assisted total knee arthroplasty and pressure sensor on early postoperative outcomes.Methods:Twenty patients who underwent YUANHUA robot-assisted TKA between September 2024 and December 2024 were prospectively enrolled. After randomization and exclusion of one patient lost to follow-up, 10 patients were included in the pressure-sensor group and 10 in the control (no-pressure) group. In the pressure-sensor group, surgeons used a pressure sensor to assist in soft tissue balancing after osteotomy, whereas in the control group, balancing was performed empirically. In both groups, medial and lateral compartment pressures of the knee at 10°, 45°, 90°, and 120° of flexion were recorded using a pressure sensor prior to component implantation. Clinical outcomes were evaluated preoperatively and at 1 day, 3 days, 1 week, 2 weeks, 6 weeks, and 3 months postoperatively using the visual analog scale (VAS), Knee Society Score (KSS), Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), and Forgotten Joint Score (FJS).Results:All 20 procedures were completed successfully, and patients were followed for at least 3 months. At 10° of knee flexion, the difference between medial and lateral compartment pressures was significantly smaller in the pressure-sensor group (0.99±59.44 N) than in the control group (97.18±84.34 N; t=-2.948, P=0.009). At 45° of flexion, the corresponding differences were -12.99±36.20 N and 51.48±76.40 N, respectively ( t=-2.411, P=0.032). No significant differences in VAS, KSS, or WOMAC scores were observed between groups preoperatively ( P>0.05). At 3 months postoperatively, the KSS was significantly higher in the pressure-sensor group (174.40±16.39) compared with the control group (138.50±38.35, t=2.722, P=0.014). The WOMAC and VAS scores were significantly lower in the pressure-sensor group (12.70±11.00 and 1.20±0.92, respectively) than in the control group (27.30±18.54 and 2.70±2.00; t=-2.142, P=0.046; t=-2.153, P=0.045). At 2 weeks and 3 months postoperatively, the FJS scores in the pressure-sensor group [70.00±26.06 and 88.07(83.52, 95.83)] were significantly higher than those in the control group [37.92±32.42 and 50.00(32.50, 67.75); t=2.439, P=0.025; Z=-2.466, P=0.014]. Conclusion:The combination of precise osteotomy using robot-assisted TKA and soft tissue balancing guided by a pressure sensor provided more accurate medial-lateral compartment balance and significantly enhanced early postoperative clinical outcomes.

Rolling circle amplification(RCA)is an efficient isothermal enzymatic reaction using a circular DNA single strand as a template and catalyzed by DNA polymerases.It is simple,fast,and efficient,and can rapidly produce ultra-long single-stranded DNA with a large number of tandem repeats in a short time.Due to the codability of DNA,template DNA sequences can be designed according to needs to result in a wide range of functional products,displaying a unique advantage in the fields of biological detection and drug delivery.This review has summarized the basic principle and classification of RCA technology,focused on the applications of RCA technology in tumor detection and drug delivery in recent years,and finally discussed the application prospects and potential challenges of this technology.

Objective To explore the effect and safety of right unilateral modified electroconvulsive therapy(RUL-MECT)for major depressive disorder patients(MDD).Methods A randomized controlled trial was conducted on 70 patients with MDD who were randomly divided into a study group and a control group.The study group underwent age-based RUL-MECT,while the control group underwent bitemporal MECT.The participants were evaluated using the 17-item Hamilton depression scale(HAMD-17),MATRICS consensus cognitive battery(MCCB)and orientation recovery tests(ORT).Any adverse reactions that occurred during each intervention process were recorded.Results Before treatment,there were no significant differences in the HAMD-17(32.89±5.68 vs.33.54±4.78)between the two groups(P>0.05).The HAMD-17 score of the intervention group was 6.83±4.68,while the control group was 7.20±4.60 after 8 interventions,repeated measures analysis of variance showed the time effect(P<0.001)was significant.The intergroup effect and interaction effect was not significant(P>0.05).In terms of MCCB scores,there were significant main effect(P<0.001)in connectivity tests,symbol coding,language memory,spatial breadth,number sequence,maze test,visual memory,emotional management and the duration of continuous operation.The intergroup effects of language memory,number sequence,visual memory,speech fluency,and continuous operation were significant(P<0.05).The interaction effect of language memory and continuous operation were significant(P<0.05).After the intervention,the recovery of orientation time was significantly shorter in the study group than that in the control group[(508.57±104.48)s vs.(631.66±212.27)s](P<0.05).The incidence of adverse reactions between two groups(28.6% vs.40.0% )has no significance(P>0.05).Conclusions Compared with bitemporal MECT,RUL-MECT has comparable efficacy in treating depressant and better performance in improving cognitive function and recovery of orientation.

Rolling circle amplification(RCA)is an efficient isothermal enzymatic reaction using a circular DNA single strand as a template and catalyzed by DNA polymerases.It is simple,fast,and efficient,and can rapidly produce ultra-long single-stranded DNA with a large number of tandem repeats in a short time.Due to the codability of DNA,template DNA sequences can be designed according to needs to result in a wide range of functional products,displaying a unique advantage in the fields of biological detection and drug delivery.This review has summarized the basic principle and classification of RCA technology,focused on the applications of RCA technology in tumor detection and drug delivery in recent years,and finally discussed the application prospects and potential challenges of this technology.

The ongoing pandemic of Coronavirus disease 19 (COVID-19) is caused by a newly discovered β Coronavirus named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). How long the adaptive immunity triggered by SARS-CoV-2 can last is of critical clinical relevance in assessing the probability of second infection and efficacy of vaccination. Here we examined, using ELISA, the IgG antibodies in serum specimens collected from 17 COVID-19 patients at 6-7 months after diagnosis and the results were compared to those from cases investigated 2 weeks to 2 months post-infection. All samples were positive for IgGs against the S- and N-proteins of SARS-CoV-2. Notably, 14 samples available at 6-7 months post-infection all showed significant neutralizing activities in a pseudovirus assay, with no difference in blocking the cell-entry of the 614D and 614G variants of SARS-CoV-2. Furthermore, in 10 blood samples from cases at 6-7 months post-infection used for memory T-cell tests, we found that interferon γ-producing CD4
Adaptive Immunity/physiology* ; Adult ; Aged ; Antibodies, Neutralizing/blood* ; COVID-19/immunology* ; Cohort Studies ; Female ; Humans ; Immunoglobulin G/blood* ; Male ; Middle Aged ; SARS-CoV-2/immunology* ; T-Lymphocytes/physiology* ; Time Factors ; Viral Proteins/immunology*

The human Shwachman-Diamond syndrome (SDS) is an autosomal recessive disease caused by mutations in a highly conserved ribosome assembly factor SBDS. The functional role of SBDS is to cooperate with another assembly factor, elongation factor 1-like (Efl1), to promote the release of eukaryotic initiation factor 6 (eIF6) from the late-stage cytoplasmic 60S precursors. In the present work, we characterized, both biochemically and structurally, the interaction between the 60S subunit and SBDS protein (Sdo1p) from yeast. Our data show that Sdo1p interacts tightly with the mature 60S subunit in vitro through its domain I and II, and is capable of bridging two 60S subunits to form a stable 2:2 dimer. Structural analysis indicates that Sdo1p bind to the ribosomal P-site, in the proximity of uL16 and uL5, and with direct contact to H69 and H38. The dynamic nature of Sdo1p on the 60S subunit, together with its strategic binding position, suggests a surveillance role of Sdo1p in monitoring the conformational maturation of the ribosomal P-site. Altogether, our data support a conformational signal-relay cascade during late-stage 60S maturation, involving uL16, Sdo1p, and Efl1p, which interrogates the functional P-site to control the departure of the anti-association factor eIF6.
Crystallography, X-Ray ; GTP Phosphohydrolases ; chemistry ; metabolism ; Humans ; Protein Domains ; Ribosome Subunits, Large, Eukaryotic ; chemistry ; metabolism ; Saccharomyces cerevisiae ; chemistry ; metabolism ; Saccharomyces cerevisiae Proteins ; chemistry ; metabolism