ObjectiveTo address the challenge of balancing efficiency and purity in isolating MSCs from rat thoracic aorta using traditional methods and to establish and validate an optimized isolation and culture system based on the dual-phase harvesting method. MethodsThoracic aortas were aseptically isolated from 3 to 4-week-old SD rats and minced. Three experimental groups were included： simple enzymatic digestion， pre-explant-enzymatic digestion sequential method， and the dual-phase harvesting method. Growth kinetic parameters， including time to initial cell migration， time to primary confluence， and cell yield， were systematically compared among the three groups. Passage‑4 cells were examined for surface marker expression （CD73， CD44， CD29， CD45， CD34， and CD11b/c） by flow cytometry. Their stemness was evaluated through osteogenic， adipogenic， and chondrogenic trilineage differentiation assays. ResultsThe time to primary confluence in the dual-phase harvesting method group （8.5 ± 1.0 ） d was significantly shorter than that of the simple enzymatic digestion group （11.2±1.2） d and the pre‑explant enzymatic digestion sequential group （12.8±1.5） d. Flow cytometric analysis revealed that the harvested cells consistently showed high expression of CD73 （87.2±1.5）%， CD44 （87.1±0.8）%， and CD29 （96.8±1.3）%， with low expression of CD45 （3.22±0.2）%， CD34 （2.46±0.5）%， and CD11b/c （2.53±2.1）%. Upon directed induction， the cells formed Alizarin Red‑positive calcium nodules， Oil Red O‑positive lipid droplets， and Alcian Blue-positive glycosaminoglycans， confirming complete trilineage differentiation potential. The cells could be stably expanded to passage 8 while maintaining a uniform elongated spindle-shaped morphology. ConclusionThe dual‑phase harvesting method established in this study is an efficient and stable optimized protocol for isolating MSCs from the rat thoracic aorta media. This approach synergistically enhances cell yield， purity， and stemness through its temporally designed two‑step process， thereby providing a high-quality in situ cell source for vascular biology research and regenerative medicine.

Objective:To analyze the medication law of Traditional Chinese Medicine (TCM) fumigation and washing to promote postoperative healing of hemorrhoids by data mining technology.Methods:The clinical literature about TCM fumigation and washing to promote postoperative healing of hemorrhoids was retrieved from the databases of CNKI, Wanfang, VIP, PubMed from the establishment of the databases to March 10, 2022. The frequency efficacy attributes, core medicinal pairs and core prescriptions of TCM were analyzed by using the Ancient and Modern Medical Records Cloud Platform (V2.3.5).Results:A total of 299 articles were included, involving 200 kinds of Chinese materia medica. The drugs used at high frequency ≥40 were Sophorae Flavescentis Radix, Phellodendri Chinensis Cortex, Natrii Sulfas, Galla Chinensis and Rhei Radix et Rhizoma and so on. The main efficacy was to clear heat and reduce dampness; cold, warm and slightly cold were the main medicinal properties, and the tastes were mainly bitter, pungent, sweet and sour, and most of the drugs return to the liver meridian, stomach meridian, heart meridian, large intestine meridian and so on. A total of 22 rules were obtained by correlation analysis. Five groups of drugs were obtained by clustering analysis. The core prescription drugs obtained by complex network analysis included Sophorae Flavescentis Radix, Phellodendri Chinensis Cortex, Natrii Sulfas, Galla Chinensis, Rhei Radix et Rhizoma, Taraxaci Herba, Borneolum Syntheticum, Sanguisorbae Radix, Atractylodis Rhizoma, Carthami Flos, Scutellariae Radix, Olibanum, Myrrha, and Lonicerae Japonicae Flos. Conclusion:TCM fumigation and washing can promote the postoperative healing of hemorrhoids mainly by clearing heat and reducing dampness and detoxification, as well as promoting blood circulation and removing blood stasis, reducing swelling and relieving pain, restraining sore and generating muscle.

Objective:To investigate the correlation between white matter hyperintensities (WMHs) and the outcomes after reperfusion therapy in patients with acute ischemic stroke (AIS).Methods:Patients with AIS treated with reperfusion therapy (intravenous thrombolysis, endovascular mechanical thrombectomy or bridging therapy) in the Stroke Center of Zhongshan Hospital of traditional Chinese Medicine from January 2014 to December 2019 were retrospectively enrolled. The clinical baseline data of the patients were collected. The Fazekas scale was used to evaluate the severity of WMHs according to the MRI images. At 90 d after discharge, the modified Rankin Scale was used to evaluate the outcomes. A score of ≤ 2 was defined as good outcome, and a score of >2 was defined as poor outcome. Binary multivariate logistic regression analysis was used to determine the independent risk factors for hemorrhagic transformation (HT), symptomatic intracranial hemorrhage (sICH), and poor outcomes. Results:A total of 676 patients with AIS treated with reperfusion therapy were enrolled. Among them, 506 patients (74.9%) were complicated with WMHs, and 80 (11.8%) had severe WMHs. One hundred and thirty-two patients (19.5%) had HT, 34 (5.0%) had sICH, and 306 (45.3%) had a poor outcome. Multivariate logistic regression analysis showed that severe WMHs was an independent risk factor for the occurrence of HT (odds ratio [ OR] 1.890, 95% confidence interval [ CI] 1.047-3.413; P=0.035) and poor outcomes ( OR 3.366, 95% CI 1.567-7.232; P=0.002) after reperfusion treatment in patients with AIS, but there was no independent correlation with sICH ( OR 8.403, 95% CI 0.891-79.294; P=0.063). Conclusion:Severe WMHs is an independent risk factor for the occurrence of HT and poor outcomes in patients with AIS after reperfusion treatment, but it has no independent correlation with sICH.

Reperfusion therapy has become a standard treatment for acute ischemic stroke, which can effectively improve the outcomes of patients and reduce the mortality. Some studies have found that reperfusion therapy may increase the incidence of post-stroke seizures and post-stroke epilepsy, but this view is still controversial. This article reviews the recent studies on reperfusion therapy and post-stroke seizures and post-stroke epilepsy.