Objective To screen and identify specific monoclonal antibodies targeting the surface envelope protein E8L of the monkeypox intracellular mature virus based on mRNA immunization and single-cell sequencing.Methods E8L-mRNA was synthesized in vitro and encapsulated into lipid nanoparticles(LNPs)for immunization to induce immune responses.The antibody level in blood serum was detected to find out when the mice had produced sufficient antibodies.B cells in the spleen of the mice were isolated by flow cytometry sorting,followed by library construction and sequencing.Based on the analysis of light and heavy chain sequences of the antibodies from the sequencing data,E8L candidate monoclonal antibodies were screened according to the abundance ranking and expressed.The affinity of these monoclonal antibodies was detected by enzyme-linked immunosorbent assay(ELISA).Candidate antibodies with high affinity for E8L were applied to immunochromatographic assays to detect the monkeypox virus(MPXV)E8L protein.Results Mice were immunized by lipid nanoparticles encapsulating E8L-mRNA.Approximately 1.5 million B cells were selected by flow cytometry.After single-cell sequencing,17 candidate monoclonal antibodies for E8L were identified and expressed.Two high affinity monoclonal antibodies were obtained for monkeypox virus E8L protein by ELISA.These two antibodies were used as the basis for immunochromatographic assays to detect E8L protein,with a sensitivity of 0.5 ng/mL.Conclusion Two monoclonal antibodies with high affinity for the MPXV E8L protein are obtained,which can be potentially used for detecting monkeypox virus.

Objective To explore the status quo of head nurses' empowerment behavior perceived by nurses and the solidarity among nurses, and to discuss the effects of head nurses' empowerment behavior on clinical nurses' solidarity. Methods Totally 500 on-the-job clinical nurses from three ClassⅢ Grade A hospitals in Wenzhou were investigated from October to December 2016 by convenient sampling. Empowering Ledership Behavior Questionnaire and Colleague Solidarity of Nurses' Scale (CSNS) were used to investigate head nurses' empowerment behavior perceived by nurses and the colleague solidarity of nurses, respectively. The data thus collected were entered into SPSS 19.0, and t test or single factor analysis of variance were conducted on the measurement data; the relationship between head nurses' empowerment behavior and colleague solidarity of nurses were analyzed by Pearson correlation analysis; and the influencing factors of colleague solidarity of nurses were analyzed by hierarchical regression analysis. Results Totally 500 questionnaires were handed out, with 432 valid questionnaires collected. The total average score of the 432 nurses for head nurses' empowerment behavior perceived by nurses was (4.05±0.46), and the total score in head nurses' empowerment behavior perceived by nurses was positively correlated with the total score in colleague solidarity of nurses (r=0.31, P<0.01); and according to the regression analysis, when controlling the demographic variables, the dimension of empowerment and personal development support could account for 34.2% of the colleague solidarity of nurses. Conclusions Head nurses' empowerment behavior is a favorable condition to improving the colleague solidarity of clinical nurses, but in the process of empowerment, the group of nurses with low annual income and poor educational background should be paid attention to so as to improve the overall solidarity.

Objective To evaluate the diagnostic valu e of ~131I-MIBG adrenomedullary scintigraphy for high blood cat echolamine. Methods 96 cases of high blood catecholamine and 197 cases of other diseases were screened with ~131I-metaiodo benzylguanidine(MIBG) adrenomedullary scintigraphy. Results 56 cases of the total 60 cases of pheochromocytoma and 33 cases of the total 36 cases of adrenomedullary hyperplasia showed positive image. The positiv e rate were 93.3% and 91.7% respectively. 138 cases of primary hypertension, 4 9 cases of adrenal and other organic tumor, 4 cases of pheochromocytoma befor e operation, 10 cases of cured pheochromocytoma after resection and 3 cases of adrenomedullary hyperplasia all showed negative image. The total positive rate for high blood catecholamine was 92.7%, which was much higher than other method s, such as biochemical assay, type B ultrasound, computer tomography and magneti c resonance image. Conclusions ~131I-MIBG w as of great value for the locative and qualitative diagnosis of high blood catec holamine,especially for the silent, ectopic and multiple pheochromocytoma, the m etastatic malignant pheochromocytoma and adrenomedullary hyperplasia. It could be used for the differential diagnosis of high blood catecholamine from primary hypertension and other kinds of tumors.