BackgroundNegative emotions are core predictors of adolescent mental health. While existing research has predominantly focused on the impact of risk factors such as negative life events and rumination on adolescents' emotions， the protective role of the family， particularly parental involvement， has received less attention. Consequently， the integrated mechanism connecting negative life events， rumination， parental involvement and negative emotions remains insufficiently clarified. ObjectiveTo elucidate the mechanisms linking negative life events to negative emotions in adolescents， and to verify the mediating role of rumination and the moderating role of parental involvement， so as to provide a theoretical basis for relevant prevention and intervention. MethodsA cross-sectional study was conducted in June 2025. A total of 600 adolescents from a middle school in Liangshan Yi Autonomous Prefecture， Sichuan Province， were recruited through cluster random sampling. Participants completed the Adolescent Self-rating Life Events Checklist （ASLEC）， the Rumination Response Scale （RRS）， parental involvement subscale of the Perception of Parents Scales （POPS）， and the Chinese short version of Depression Anxiety and Stress Scale （DASS-21）. Pearson correlation analysis was performed to examine the correlations among all variables. Model 4 and model 7 embedded in Process macro 4.1 were employed to separately test the mediating effect of rumination between negative life events and negative emotions of adolescents， as well as the moderating effect of parental involvement. ResultsUltimately， 534 valid questionnaires were obtained， yielding a valid response rate of 89.00%. ASLEC score and RRS score were positively correlated with DASS-21 scores （r=0.580， 0.706， P<0.01）， and ASLEC score was also positively correlated with RRS score （r=0.583， P<0.01）. Rumination acted as a partial mediator between negative life events and negative emotions， with an indirect effect of 0.531 （95% CI： 0.438–0.630）， accounting for 55.60% of the total effect. Parental involvement significantly moderated the first stage of the mediating pathway （β=-0.109， P<0.05）. ConclusionIn adolescents， rumination serves as a partial mediator in the relationship between negative life events and negative emotions， and parental involvement further moderates the predictive relationship from negative life events to rumination.

Objective To explore the correlation between serum iron levels and granulomatous lobular mastitis(GLM).Methods A total of 102 patients with GLM were admitted from May 2022 to May 2023.According to the disease course stage at the time of the patients' visit,they were divided into the mass stage group(29 cases),the abscess stage group(48 cases),and the remission stage group(25 cases).Compare the serum iron levels of patients with GLM at different stages.Spearman correlation analysis was used to analyze the correlation between serum iron levels and inflammatory indicators,and the changes in serum iron levels of patients before and after treatment remission were compared through follow-up.Results All the 102 GLM patients were female.The serum iron levels of patients in the abscess stage group,the mass stage group and the remission stage group were 12.8(10.20,17.50)μmol/L,12.8(10.20,17.50)μmol/L and 15.4(13.65,18.50)μmol/L,respectively.The abscess stage group was lower than the mass stage group and the remission stage group,and the difference was statistically significant(P＜0.001).The results of bivariate Spearman correlation analysis showed that the serum iron levels were moderately negatively correlated with white blood cell count,neutrophil count and the percentage of neutrophils(r=-0.336,P=0.001;r=-0.361,P=0.001;r=-0.334,P＜0.01),low intensity negative correlation with monocyte count(r=-0.214,P=0.031),moderately positively correlated with the percentage of lymphocytes,the levels of interleukin(IL)-2,IL-4,IL-10(r=0.328,P＜0.01;r=0.494,P=0.023;r=0.514,P=0.017;r=0.478,P=0.028).Serum iron levels in 45 GLM follow-up patients after treatment remission were higher than those before remission[(15.09±4.78)μmol/L vs.(10.64±4.47)μmol/L,P＜0.001].Conclusion Serum iron level is associated with disease severity,inflammatory markers such as immune cells and cytokines,and disease outcome of GLM.

Objective To explore the correlation between serum iron levels and granulomatous lobular mastitis(GLM).Methods A total of 102 patients with GLM were admitted from May 2022 to May 2023.According to the disease course stage at the time of the patients' visit,they were divided into the mass stage group(29 cases),the abscess stage group(48 cases),and the remission stage group(25 cases).Compare the serum iron levels of patients with GLM at different stages.Spearman correlation analysis was used to analyze the correlation between serum iron levels and inflammatory indicators,and the changes in serum iron levels of patients before and after treatment remission were compared through follow-up.Results All the 102 GLM patients were female.The serum iron levels of patients in the abscess stage group,the mass stage group and the remission stage group were 12.8(10.20,17.50)μmol/L,12.8(10.20,17.50)μmol/L and 15.4(13.65,18.50)μmol/L,respectively.The abscess stage group was lower than the mass stage group and the remission stage group,and the difference was statistically significant(P＜0.001).The results of bivariate Spearman correlation analysis showed that the serum iron levels were moderately negatively correlated with white blood cell count,neutrophil count and the percentage of neutrophils(r=-0.336,P=0.001;r=-0.361,P=0.001;r=-0.334,P＜0.01),low intensity negative correlation with monocyte count(r=-0.214,P=0.031),moderately positively correlated with the percentage of lymphocytes,the levels of interleukin(IL)-2,IL-4,IL-10(r=0.328,P＜0.01;r=0.494,P=0.023;r=0.514,P=0.017;r=0.478,P=0.028).Serum iron levels in 45 GLM follow-up patients after treatment remission were higher than those before remission[(15.09±4.78)μmol/L vs.(10.64±4.47)μmol/L,P＜0.001].Conclusion Serum iron level is associated with disease severity,inflammatory markers such as immune cells and cytokines,and disease outcome of GLM.

Objective To analyze the interaction between PML protein and TAB1 protein using bioinformatic approaches and experimentally verify the results.Methods Using Rosetta software,a 3D model of TAB1 protein was constructed through a comparative modeling approach;the secondary structure of PML protein was retrieved in the PDB database and its crystal structure and 3D structure were resolved.Zdock 3.0.2 software was used to perform protein-protein docking of PML and TAB1,and the best conformation was extracted for molecular structure analysis of the docking model.The interaction between the two proteins was detected using immunoprecipitation in α-MMC-treated M1 inflammatory macrophages.Results When 6IMQ of PML was used as the docking site,PML protein formed 3 salt bridges,6 hydrogen bonds and 6 hydrophobic interactions with TAB1 proteins;when 5YUF of PML was used as the docking site,PML protein formed 1 hydrogen bond,3 electrostatic interactions and 9 hydrophobic interactions with TAB1 proteins,and both of the docking modes formed good molecular docking and interactions.In the M1 inflammatory macrophages treated with α-MMC for 4 h,positive protein bands of PML and TAB1 were detected in the cell lysates in PML-IP group.Conclusion PML protein can interact strongly with TAB1 protein.

Objective To analyze the interaction between PML protein and TAB1 protein using bioinformatic approaches and experimentally verify the results.Methods Using Rosetta software,a 3D model of TAB1 protein was constructed through a comparative modeling approach;the secondary structure of PML protein was retrieved in the PDB database and its crystal structure and 3D structure were resolved.Zdock 3.0.2 software was used to perform protein-protein docking of PML and TAB1,and the best conformation was extracted for molecular structure analysis of the docking model.The interaction between the two proteins was detected using immunoprecipitation in α-MMC-treated M1 inflammatory macrophages.Results When 6IMQ of PML was used as the docking site,PML protein formed 3 salt bridges,6 hydrogen bonds and 6 hydrophobic interactions with TAB1 proteins;when 5YUF of PML was used as the docking site,PML protein formed 1 hydrogen bond,3 electrostatic interactions and 9 hydrophobic interactions with TAB1 proteins,and both of the docking modes formed good molecular docking and interactions.In the M1 inflammatory macrophages treated with α-MMC for 4 h,positive protein bands of PML and TAB1 were detected in the cell lysates in PML-IP group.Conclusion PML protein can interact strongly with TAB1 protein.

Objective: To explore the effects and mechanisms of interfering with transient receptor potential melastatin subfamily member 7(TRPM7) on biological behaviors(proliferation, apoptosis, invasion) of laryngeal carcinoma cells. Methods: Human laryngeal carcinoma cells TU212 were culturedin vitro. TRPM7-shRNA plasmid vectors(TRPM7-shRNA1 group, TRPM7-shRNA2 group, TRPM7-shRNA3 group) and negative control shRNA-NC(shRNA-NC group) were constructed. TU212 cells were transfected by liposome transfection method. The cells transfected with empty vector were enrolled as Control group. The level of lactic dehydrogenase(LDH) in TU212 supernatant was detected by ELISA. The level of malondialdehyde(MDA) and activity of superoxide dismutase(SOD) in supernatant were detected by colorimetry. The effects of knocking-down TRPM7 on proliferation and invasion of TU212 cells were detected by CCK-8, clone formation assay and Transwell assay. The expressions of invasion and apoptosis-related proteins were detected by Western blot. Results: After transfection, expression levels of TRPM7 mRNA and protein were down-regulated in TRPM7-shRNA1 group, TRPM7-shRNA2 group and TRPM7-shRNA3 group compared with Control group(P<0.05), and the decrease was the most significant in TRPM7-shRNA1 group(P<0.05). In functional experiments, SOD level in TRPM7-shRNA1 group decreased compared with Control group(P<0.05), while MDA and LDH levels increased(P<0.05). The cells proliferation rate and clone formation rate were decreased(P<0.05), the number of invasion cells was reduced(P<0.05), the expressions of N-cadherin and Vimentin proteins were down-regulated(P<0.05), mitochondrial membrane potentials were reduced(P<0.05), Bax/Bcl-2 and cleaved caspase-3/caspase-3 increased(P<0.05). Conclusion Knocking-down TRPM7 can increase oxidative stress level in laryngeal carcinoma cells TU212, inhibit their proliferation and invasion, and induce their apoptosis by mitochondrial pathways.

Objective:To investigate the risk factors for anastomotic leakage after laparo-scopic lower anterior resection (LAR) of rectal cancer, and the application value of its risk assess-ment scoring model.Methods:The retrospective case-control study was conducted. The clinico-pathological data of 539 patients who underwent laparoscopic LAR of rectal cancer in 13 medical centers, including 248 cases in Renji Hospital of Shanghai Jiaotong University School of Medicine, 35 cases in Ningbo First Hospital, 35 cases in Changzhou Second People's Hospital, 32 cases in the First People's Hospital of Nantong, 32 cases in Linyi People's Hospital, 31 cases in Changzhou Wujin People's Hospital, 28 cases in Jiading District Hospital of Traditional Chinese Medicine, 27 cases in the First Hospital of Taizhou, 26 cases in Shanghai Pudong Gongli Hospital, 21 cases in the People's Hospital of Rugao, 11 cases in Central Hospital of Fengxian District, 7 cases in Ningbo Hangzhou Bay Hospital and 6 cases in Jiangsu jianhu People's Hospital, from January 2016 to November 2020 were collected. There were 157 males and 382 females, aged (62.7±0.5)years. Observation indicators: (1) follow-up; (2) risk factors for anastomotic leakage after laparoscopic LAR; (3) establishment of risk assessment scoring model for anastomotic leakage after laparoscopic LAR. Follow-up was conducted by outpatient examination or telephone interview. Patients were followed up at 1 week after discharge or 1 month after the operation to detect the anastomotic leakage. Measurement data with normal distribution were represented as Mean± SD, and measurement data with skewed distribution were represented as M(range). Count data were represented as absolute numbers or percentages, and comparison between groups was analyzed using the chi-square test. Univariate analysis was conducted using the chi-square test and multivariate analysis was conducted usong the Logistic regression model. The area under curve of receiver operating characteristic curve was used to estimate the efficiency of detecton methods. The maximum value of the Youden index was defined as the best cut-off value. Results:(1) Follow-up: 539 patients were followed up at postoperative 1 week and 1 month. During the follow-up, 79 patient had anastomotic leakage, with an incidence of 14.66%(79/539). Of the 79 patients, 39 cases were cured after conservative treatment, 40 cases were cured after reoperation (ileostomy or colostomy). (2) Risk factors for anastomotic leakage after laparoscopic LAR. Results of univariate analysis showed that sex, age, body mass index, smoking and/or drinking, tumor diameter, diabetes mellitus, hemoglobin, albumin, grade of American Society of Anesthesio-logists (ASA), neoadjuvant chemoradiotherapy, distance from anastomotic level to dentate line, the number of pelvic stapler, reinforced anastomosis, volume of intraoperative blood loss, placement of decompression tube, preservation of left colic artery, operation time and professional doctors were related factors for anastomotic leakage after laparoscopic LAR ( χ2=14.060, 4.387, 5.039, 4.094, 17.488, 33.485, 25.066, 28.959, 34.973, 34.207, 22.076, 13.208, 16.440, 17.708, 17.260, 4.573, 5.919, 5.389, P<0.05). Results of multivariate analysis showed that male, tumor diameter ≥3.5 cm, diabetes mellitus, hemoglobin <90 g/L, albumin <30 g/L, grade of ASA ≥Ⅲ, neoadjuvant chemoradiotherapy, distance from anastomotic level to dentate line <1 cm, the number of pelvic stapler ≥3, non-reinforced anastomosis, volume of intraoperative blood loss ≥100 mL and no placement of decom-pression tube were independent risk factors for anastomotic leakage after laparoscopic LAR ( odds ratio=2.864,3.043,12.556,7.178,8.425,12.895,8.987,4.002,3.084,4.393,3.266,3.224,95% confidence interval as 1.279?6.411, 1.404?6.594, 4.469?35.274, 2.648?19.459, 2.471?28.733, 4.027?41.289, 3.702?21.777, 1.746?9.171, 1.365?6.966, 1.914?10.083, 1.434?7.441, 1.321?7.867, P<0.05). (3) Establishment of risk assessment scoring model for anastomotic leakage after laparoscopic LAR. based on the results of univariate analysis, clinicopathological factors with χ2>20, χ2>10 and ≤20 or χ2≤10 were defined as scoring of 3, 2, 1, respectively. The cumulative clinicopatho-logical factors scoring ≥6 was defined as an effective evaluating indicator for postoperative anastomotic leakage. The risk assessment scoring model (6-321) for anastomotic leakage after laparoscopic LAR was established. The cumulative value ≥6 indicated high incidence of anastomotic leakage, and the cumulative value <6 indicated low incidence of anastomotic leakage. Conclusions:Male, tumor diameter ≥3.5 cm, diabetes mellitus, hemoglobin <90 g/L, albumin <30 g/L, grade of ASA ≥Ⅲ, neo-adjuvant chemoradiotherapy, distance from anastomotic level to dentate line <1 cm, the number of pelvic stapler ≥3, non-reinforced anastomosis, volume of intraoperative blood loss ≥100 mL and no placement of decompression tube are independent risk factors for anastomotic leakage after laparoscopic LAR. The risk assessment scoring model (6-321) is established according to the above results.The cumulative value ≥6 indicates high incidence of anastomotic leakage and the cumulative value <6 indicates low incidence of anastomotic leakage.

Objective To understand the fluorescence pattern of antinuclear antibody(ANA)and the distribution of target anti-gen and ANA′s impact on rheumatoid arthritis(RA)RA.Methods The data of rheumatoid factor(RF)and anti-cyclic citrullinated peptide antibodies(anti-CCP antibodies)and ANA of 860 RA patients during 2014-2015 were collected and divided into 2 groups according to the ANA levels.The differences of RF and anti-CCP antibodies level between 2 groups were analyzed.Analyzed the fluorescence pattern of RA merger ANA and the distribution of target antigen by statistical.Results Of the 860 RA patients,the positive rate of ANA was 37.6%,the major fluorescence pattern of RA merger ANA was speckled pattern,anti SSA positive rate was 21.7%.The RF and anti-CCP antibodies level had statistical difference between ANA positive group and ANA negative group (P <0.05).Conclusion RA merger ANA positive can show different fluorescence patterns and target antigen,the speckled pattern and anti-SSA positive are the main pattern.The severity of arthritis symptoms and the degree of bone destruction between ANA positive group and ANA negative group were difference.Anti-ANA positive has a certain promoting effect on the progression of RA disease.

Objective To explore the attachment of children with emotional disorder,and provide a theoretical basis for mental intervention.Methods A total of 70 outpatients with emotional disorder and their parents and 140 normal children and their parents were enrolled.All the children and their parents were assessed with Adolescent Attachment Inventory and Experiences in Close Relationships Inventory and General demographic information inventory.Results There were statistical significances in family proximity((12.76 ± 3.69) vs (15.47 ±3.05)),friend proximity ((14.23 ± 3.84) vs (15.82 ± 3.06)) and family negativity ((12.84 ± 3.42) vs (10.46 ± 2.94)) of two groups (all P ＜ 0.05).There were statistical significances in maternal attachment avoidance ((3.60 ± 0.70) vs (3.84 ± 0.63)) and anxiety ((3.23 ± 0.77) vs (3.37 ± 0.79)) of two groups (all P ＜0.05).In emotional disorder group,there was a significantly negative relation (P ＜ 0.01) between parental anxiety dimension and friend dependence factor.Conclusion The attachment of children with emotional disorser is low family and friends proximity,higher family negativity.

In order to obtain salt-tolerant calli of Dandelion (Taraxacum officinale Weber), calli were induced from leaf explants of Dandelion on Murashige and Skoog's medium supplemented with 2.0 mg/L 6-benzyladenine and 0.5 mg/L 2,4-dichlorophen oxyacetic acid With 1.5% NaCl as selection pressure, most calli became brown and dead, whereas some new cell clusters appeared at the edge of the brown calli after 2 to 3 weeks. The survived cells were picked out and sub-cultured every 3 weeks onto the fresh selection medium and salt-tolerant calli were finally obtained through 4 continuous selections on the selection medium supplemented with 1.5% NaCl. Salt-tolerant calli increased steadily under a fixed NaCl stress though their relative growth rate decreased with increased NaCl concentration whereas the control calli which were sub-cultured by 4 continus selections on salt free medium ceased to grow under the same condition. This result indicated that the salt-tolerance of the selected calli is improved and this character is stable. Compared with the control, the SDS-PAGE pattern of the salt-tolerant calli had a unique 34 kD protein band. Its 30 kD and 18 kD protein bands were up-regulated. Further more, within the NaCl stress range up to 1.5%, the activities of antioxidant enzymes such as super oxidase dimutase, peroxidase and catalase, and the proline contents of the salt-tolerant calli were higher than those of the control. The results indicated that the selected calli with improved and stable salt tolerance were cell variants. The accumulation of the organic compatible solutes including proteins and the enhanced antioxidant capabilities in the salt tolerant calli are the two ways for them to regulate their osmotic homeostasis and alleviate the secondary reactive oxygen spexies damage respectively.
Adaptation, Physiological ; Cell Culture Techniques ; methods ; Drug Tolerance ; physiology ; Salt-Tolerant Plants ; genetics ; growth & development ; physiology ; Sodium Chloride ; pharmacology ; Stress, Physiological ; Taraxacum ; genetics ; growth & development ; physiology