The senescence accelerate mouse prone strain was identified by Professor T.Takeda's laboratory in Japan from the offspring produced by the brother-sister inbred line of AKR/J mice donated by Jackson Laboratory in the United States SAMP),and SAMP8 mice showed a variety of aging characteristics,such as:Pathological changes such as memory and learning disorders,behavioral abnormalities,skin roughness,aging,hair loss,eye lesions(visual impairment,cataract,periocular lesions),hearing impairment,weight loss,muscle strength loss,reproductive system aging,liver and fat metabolism disorders,etc.,are widely used in experimental studies of related diseases or pathology,and have made considerable contributions to the research of diseases.However,there are also many problems in its application,such as the selection of month age,the use of intervention methods,the suitability of pathological changes and research content,and the understanding of models under different theoretical backgrounds,which are of great research value.Therefore,this paper systematically analyzes the above problems based on existing literature,in order to provide relevant basis for the application of SAMP8 mice in different fields and different mechanisms.

The senescence accelerate mouse prone strain was identified by Professor T.Takeda's laboratory in Japan from the offspring produced by the brother-sister inbred line of AKR/J mice donated by Jackson Laboratory in the United States SAMP),and SAMP8 mice showed a variety of aging characteristics,such as:Pathological changes such as memory and learning disorders,behavioral abnormalities,skin roughness,aging,hair loss,eye lesions(visual impairment,cataract,periocular lesions),hearing impairment,weight loss,muscle strength loss,reproductive system aging,liver and fat metabolism disorders,etc.,are widely used in experimental studies of related diseases or pathology,and have made considerable contributions to the research of diseases.However,there are also many problems in its application,such as the selection of month age,the use of intervention methods,the suitability of pathological changes and research content,and the understanding of models under different theoretical backgrounds,which are of great research value.Therefore,this paper systematically analyzes the above problems based on existing literature,in order to provide relevant basis for the application of SAMP8 mice in different fields and different mechanisms.

Objective To explore a kind of comfortable medical for ervical plexus anesthesia auxiliary anesthesia .Methods 80 thyroidectomy female patients were randomly divided into normal saline group (group A) ,droperidol-fentanyl group (group B) ,bu-torphanol group (group C) ,dex group (group D) ,20 cases in each group .The change of systolic blood pressure (SBP) ,diastolic blood pressure (DBP) ,heart rate (HR) ,blood oxygen saturation (SpO2 ) ,narcotrend index (NTI) were monitored .The above pa-rameters were recorded at 10 min before cervical plexus(T0 ) ,cervical plexus operation(T1 ) ,skin incision(T2 ) ,separation of thyroid upper extreme(T3 )and suture(T4 ) .Results There was no significant difference between the groups A and B at the same time point (P>0 .05) .In group C ,the SBP、DBP、HR at T3 and T4 were significant higher than at T0 ,and at the same point the A and B group decreased statistically significant (P<0 .05) .In group D ,the SBP、DBP、HR at T2 、T3 、T4 were significant lower than at T1 ,espe-cially at T4 compared to at T0 (P<0 .05) .Compared to group A ,B ,C ,the SBP、DBP、HR in group D were lower at T3 and T4(P<0 .05) .NTI in group D at T1 、T2 had significant difference compared with group A and B (P<0 .05) ,especially at T3 、T4 were sta-tistically significant than the other three groups(P<0 .05) ,sedation was better than the other groups .Conclusion Cervical plexus block supplemented by dex can reduce the patient′s stress response and reach moderate sedation analgesia to comfortable medical purposes .

In order to study the cytocompatibility of nanophase hydroxyapatite ceramic in vitro, we prepared hydroxyapatite by use of the wet chemistry techniques. The grain size of hydroxyapatite of interest to the present study was determined by scanning electron microscopy and atomic force microscopy with image analysis software. Primary culture of osteoblast from rat calvaria was established. Protein content, synthesis of alkaline phosphatase and deposition of calcium-containing mineral by osteoblasts cultured on nanophase hydroxyapatite ceramics and on conventional hydroxyapatite ceramics for 7, 14, 21 and 28 days were examined. The results showed that the average surface grain size of the nanophase and that of the conventional HA compact formulations was 55 (nanophase) and 780 (conventional) nm, respectively. More importantly, compared to the synthesis of alkaline phosphatase and deposition of calcium-containing mineral by osteoblasts cultured on nanophase was significantly greater than that on conventional ceramics after 21 and 28 days. The cytocompatibility was significantly greater on nanophase HA than on conventional formulations of the same ceramic.
Alkaline Phosphatase ; metabolism ; Animals ; Biocompatible Materials ; chemistry ; Cell Adhesion ; Cells, Cultured ; Ceramics ; chemistry ; Durapatite ; chemistry ; Microscopy, Atomic Force ; Microscopy, Electron, Scanning ; Nanostructures ; chemistry ; Osteoblasts ; cytology ; Rats

Objective: To study the cytocompatibility of nanophase alumina ceramics with osteoblasts. Methods: Alumina ceramics were prepared via wet chemistry techniques. The grain size of alumina of interest in the present study was determined by scanning electron microscopy and atomic force microscopy with image analysis software. Primary osteoblast culture was established from rat calvaria. Protein content, synthesis of alkaline phosphatase(ALP) and deposition of calcium-containing mineral by osteoblasts cultured on nanophase alumina ceramics and conventional alumina ceramics for 7, 14, 21 and 28 d were respectively examined. Results: The average surface grain size of the nanophase and conventional alumina compact formulations was 60 nm and 1.80 ?m respectively.Synthesis of ALP and deposition of calcium-containing mineral were significantly greater by osteoblasts cultured on nanophase than those on conventional ceramics after 21- and 28- day culture. Conclusions: Nanophase alumina may stimulate ALP synthesis and calcium deposition of osteoblasts.