Objective : To investigate the role of endoplasmic reticulum stress in the occurrence and development of fatty liver induced by high fat. Methods : In the high-fat Drosophila model, the high-fat group was fed with high-fat medium, while the control group was fed with normal medium; in the mouse fatty liver model, the high-fat group was fed with high-fat diet, and the control group was fed with normal diet; in the HepG2 cell steatosis model, the high-fat group was induced by palmitic acid(PA), and the control group was cultured with DMEM. The fat body size of the third instar larvae of Drosophila melanogaster was photographed. Steatosis in mice liver and HepG2 cells was observed by H&E and Oil Red staining. The expression levels of ATF6, Bip and CHOP in the third instar larvae, liver tissues of mice and HepG2 cells were analyzed by quantitative real-time polymerase chain reaction(qPCR) and Western blot. Results : In Drosophila model, fat body and fat storage were obviously increased in high fat fed flies when compared with control group. The formation of liver fat droplets and cells vacuolation were confirmed by H&E and Oil Red staining in mice livers fed with high fat and HepG2 cells with palmitic acid treatment. The expression levels of ATF6, Bip and CHOP were significantly increased in third instar larvae and mice livers fed with high fat and palmitic acid treated HepG2 cells with palmitic acid treatment. Conclusion High fat may induce the occurrence and development of hepatic steatosis by activating endoplasmic reticulum stress.

Objective:To study the effects of hyperbaric oxygen (HBO) treatment on partial pressure of oxygen (PaO 2) and malignant biological behavior of gastric cancer cell line SGC7901. Methods:After culture, the gastric cancer cell line SGC7901 was divided into HBO group and control group. The control group did not receive any treatment, while the SGC7901 in the HBO group was treated with HBO. The PaO 2 in cell culture medium was measured by blood gas analyzer. Morphological changes of SGC7901 cells before and after HBO treatment were observed by an inverted microscope. The cell apoptosis after HBO treatment was detected by Hoechst33258 staining. The expression level of HIF-1α protein in SGC7901 cells after HBO treatment was detected by Western blotting. Results:The blood gas analyzer can accurately detect the PaO 2 of cell culture medium, and the PaO 2 of cell culture medium in the HBO group was significantly higher than that in the control group ( P<0.05); moreover, the PaO 2 of cell culture medium in the HBO group decreased slowly at different time points. After HBO treatment, the morphology of SGC7901 cells became round and the intercellular connection was loose. The Hoechst33258 staining showed that, after HBO treatment, no significant cell apoptosis was observed in SGC7901 cells in the HBO group as compared with the control group ( P>0.05). Western blotting results showed that the expression level of HIF-1α in the HBO group was significantly lower than that in the control group ( P<0.05). Conclusion:HBO treatment can obviously increase the PaO 2 in the culture medium of gastric cancer cell line SGC7901, effectively relieve the hypoxia state of SGC7901 cells, and down-regulate the expression of HIF-1α protein.

Objective:To discuss the effect of hyperbaric oxygen (HBO) combined with melatonin (MLT) on the migration ability of lung adenocarcinoma A549 cells and its possible molecular mechanism.Methods:After the cell culture, lung adenocarcinoma A549 cells were divided into cell control group (control group), hyperbaric oxygen treatment group (HBO group), melatonin treatment group (MLT group), and hyperbaric oxygen combined with melatonin group (MLT+ HBO group). Wound healing assay was used to assess the migration ability of A549 cells, and Western blotting was used to detect the expression of migration-related protein matrix metalloproteinase 9 (MMP-9) in each group.Results:The migration distances of lung adenocarcinoma A549 cells in the MLT group and the MLT+ HBO group after 48 h were shorter than that in the control group, with statistically significant differences ( P<0.01); and the migration distance of lung adenocarcinoma A549 cells in the MLT+ HBO group was reduced even more obvious than that in the MLT group, and the difference was statistically significant ( P<0.01). The expression levels of MMP-9 in the HBO group and the MLT group were lower than that of the control group ( P<0.05); the expression level of MMP-9 in the MLT+ HBO group was even lower than those in the HBO group and the MLT group, and the difference was statistically significant ( P<0.05). Conclusion:Melatonin can inhibit the migration ability of lung adenocarcinoma A549 cells, and hyperbaric oxygen can enhance such inhibitory effect. The mechanism of action may be related to the reduced expression of migration-related protein MMP-9.

Objective:To study the effects of hyperbaric oxygen (HBO) treatment on partial pressure of oxygen (PaO 2) and malignant biological behavior of gastric cancer cell line SGC7901. Methods:After culture, the gastric cancer cell line SGC7901 was divided into HBO group and control group. The control group did not receive any treatment, while the SGC7901 in the HBO group was treated with HBO. The PaO 2 in cell culture medium was measured by blood gas analyzer. Morphological changes of SGC7901 cells before and after HBO treatment were observed by an inverted microscope. The cell apoptosis after HBO treatment was detected by Hoechst33258 staining. The expression level of HIF-1α protein in SGC7901 cells after HBO treatment was detected by Western blotting. Results:The blood gas analyzer can accurately detect the PaO 2 of cell culture medium, and the PaO 2 of cell culture medium in the HBO group was significantly higher than that in the control group ( P<0.05); moreover, the PaO 2 of cell culture medium in the HBO group decreased slowly at different time points. After HBO treatment, the morphology of SGC7901 cells became round and the intercellular connection was loose. The Hoechst33258 staining showed that, after HBO treatment, no significant cell apoptosis was observed in SGC7901 cells in the HBO group as compared with the control group ( P>0.05). Western blotting results showed that the expression level of HIF-1α in the HBO group was significantly lower than that in the control group ( P<0.05). Conclusion:HBO treatment can obviously increase the PaO 2 in the culture medium of gastric cancer cell line SGC7901, effectively relieve the hypoxia state of SGC7901 cells, and down-regulate the expression of HIF-1α protein.

Objective:To discuss the effect of hyperbaric oxygen (HBO) combined with melatonin (MLT) on the migration ability of lung adenocarcinoma A549 cells and its possible molecular mechanism.Methods:After the cell culture, lung adenocarcinoma A549 cells were divided into cell control group (control group), hyperbaric oxygen treatment group (HBO group), melatonin treatment group (MLT group), and hyperbaric oxygen combined with melatonin group (MLT+ HBO group). Wound healing assay was used to assess the migration ability of A549 cells, and Western blotting was used to detect the expression of migration-related protein matrix metalloproteinase 9 (MMP-9) in each group.Results:The migration distances of lung adenocarcinoma A549 cells in the MLT group and the MLT+ HBO group after 48 h were shorter than that in the control group, with statistically significant differences ( P<0.01); and the migration distance of lung adenocarcinoma A549 cells in the MLT+ HBO group was reduced even more obvious than that in the MLT group, and the difference was statistically significant ( P<0.01). The expression levels of MMP-9 in the HBO group and the MLT group were lower than that of the control group ( P<0.05); the expression level of MMP-9 in the MLT+ HBO group was even lower than those in the HBO group and the MLT group, and the difference was statistically significant ( P<0.05). Conclusion:Melatonin can inhibit the migration ability of lung adenocarcinoma A549 cells, and hyperbaric oxygen can enhance such inhibitory effect. The mechanism of action may be related to the reduced expression of migration-related protein MMP-9.

Objective: To explore the influencing factors of rapid postoperative recovery in young(≤40 years old) lung cancer patients. Methods: Retrospective analysis was performed on 82 young patients with lung cancer diagnosed by postoperative pathology admitted to the department of thoracic surgery of the first affiliated hospital of Zhengzhou University from March 2013 to March 2019, the patients were divided into two groups according to their postoperative hospitalization time(hospitalization time≤7d, hospitalization time >7d). The preoperative medical history and examination data, intraoperative(operative method, embedding materials), postoperative complications and postoperative treatment and other data of the enrolled patients were collected to analyze the relationship between various factors and postoperative hospitalization time.Univariate analysis used t test or Fisher exact probability method, multivariate analysis used logistic regression model to analyze the data . Results: All 82 patients successfully completed the operation, and no death occurred during the perioperative period. There were no significant differences(P>0.05)according to the two groups of patients in the preoperative pulmonary function(FEV1) operation history, history of hypertension, diabetes, history of preoperative chemotherapy and surgery in the patients' position, blood transfusion, pleural adhesion, Czech, nai d, the use of xanthan gum, operation time, the maximum diameter and postoperative tumor thermal perfusion, fever, vomiting, choking cough, abdominal distension, etc.And it has significant differences(P<0.05). In the preoperative antibiotic use(P=0.002), the improvement of lung function(P=0.018), smoking history(P=0.024), medical reasons(P=0.011) and the operation(P<0.001), the lymph node excision scope(P<0.001), the lymph node dissection(P=0.017), hemostatic material use(P=0.023), blood loss(P=0.001) and postoperative average white blood cell count(P=0.033). Conclusion Preoperative and postoperative prophylactic use of antibiotics and drugs to improve pulmonary function were beneficial to postoperative recovery.Smoking was an independent risk factor for prolonged postoperative hospital stay.Minimally invasive operation and application of hemostatic materials can effectively shorten the postoperative hospitalization time of patients.

Objective To investigate the effects of hyperbaric oxygen (HBO) on the melatonin enhancement of the differentiation of gastric cancer cells SGC7901 and also to explore the possible mechanism involved.Methods The SGC7901 cells at logarithmic differentiation stage were divided into the control group,the melatonin treatment group and the HBO coupled with melatonin group.The rate of cell differentiation was detected by flow cytemctry,and endocan expression was measured by using Western blot assay.Results SGC7901 cells treated with melatonin could enhance the rate of the cells at differential stage (the rate of the melatonin treatment group was 74.35％ ±0.88％,while that of the control group was 73.12％ ± 1.01％).Melatonin treatment could also up-regulate the expression level of endocan cells (the expression level of the melatonin treatment group was 2.14 ± 0.12,while that of the control group was 1.99 ± 0.04).However,when treated with HBO + melatonin,both the rate of the cells at the differential stage (the rate of the cells of the HBO coupled with melatonin group was 76.86％ ± 1.13％) and the expression of endocan cells (the rate of endocan cells of the HBO coupled with melatonin group was 2.57 ± 0.03) were further enhanced,and statistical significance could be noted,when compared with those of the control group (P ＜ 0.05).Conclusions HBO treatment could obviously enhance the effect of melatonin on the differentiation of gastric cancer cells SGC7901,the mechanism of which might be associated with the up-regulation of endocan cells by melatonin.

Objective To investigate the effects of hyperbaric oxygen (HBO) on the melatonin enhancement of the differentiation of gastric cancer cells SGC7901 and also to explore the possible mechanism involved.Methods The SGC7901 cells at logarithmic differentiation stage were divided into the control group,the melatonin treatment group and the HBO coupled with melatonin group.The rate of cell differentiation was detected by flow cytemctry,and endocan expression was measured by using Western blot assay.Results SGC7901 cells treated with melatonin could enhance the rate of the cells at differential stage (the rate of the melatonin treatment group was 74.35％ ±0.88％,while that of the control group was 73.12％ ± 1.01％).Melatonin treatment could also up-regulate the expression level of endocan cells (the expression level of the melatonin treatment group was 2.14 ± 0.12,while that of the control group was 1.99 ± 0.04).However,when treated with HBO + melatonin,both the rate of the cells at the differential stage (the rate of the cells of the HBO coupled with melatonin group was 76.86％ ± 1.13％) and the expression of endocan cells (the rate of endocan cells of the HBO coupled with melatonin group was 2.57 ± 0.03) were further enhanced,and statistical significance could be noted,when compared with those of the control group (P ＜ 0.05).Conclusions HBO treatment could obviously enhance the effect of melatonin on the differentiation of gastric cancer cells SGC7901,the mechanism of which might be associated with the up-regulation of endocan cells by melatonin.