Objective: To investigate the clinical efficacy and safety of pudendal nerve modulation (PNM) in the treatment of female pudendal neuralgia (PN)，so as to promote the clinical application of this technique. Methods: A retrospective analysis was conducted on 20 female PN patients who failed conservative treatment at Gongli Hospital during Nov.2020 and Oct.2023.All patients underwent simultaneous PNM and sacral nerve modulation (SNM) with the assistance of 3D printing navigation.Dual-stage test electrodes for PNM and SNM were implanted，followed by alternate therapeutic trial for each modality.Secondary conversion rates and longitudinal outcomes，including visual analogue score (VAS)，patient health questionnaire-9 (PHQ-9)，and quality of life (QoL) scores were compared preoperatively，post-stage Ⅰ，and at 3，6，and 12 months post-stage Ⅱ. Results: All operations were successful.After the trial phase，the secondary conversion rate for PNM was significantly higher than that for SNM; 16 patients (16/20，80%) chose the second-phase PNM implantation surgery，3 (3/20，15%) chose second-phase SNM implantation，and 1 (1/20，5%) had electrodes removed due to ineffective results from both trials.Further assessment revealed that the improvements in VAS，PHQ-9，and QoL scores for PNM patients were significantly better than those for SNM patients after the first phase of surgery and at 3，6 and 12 months after the second-phase conversion (P<0.05).No complications such as electrode migration or infection were observed during the follow-up of 12-15 months. Conclusion: PNM provides more effective relief of pain symptoms and improvements in depressive states for female PN patients compared to SNM.With the assistance of 3D printing navigation，the operation is simple and safe，and offers stable therapeutic effects.It is worthy of clinical promotion and application.

Objective:To identify the relevant factors for the first-course remission of acute myeloid leukemia (AML) and to develop a predictive model as well as assess its predictive capability.Methods:Clinical data of 749 patients newly diagnosed with AML admitted to the Department of Hematology, the First Affiliated Hospital, Zhejiang University, School of Medicine from January 1, 2019, to April 30, 2023, were collected and randomly divided into training and validation sets. Multivariate logistic regression analysis was conducted to determine variables associated with complete remission in the first course of induction therapy, and a predictive model was established based on these variables. The receiver operating characteristic (ROC) curve of the predictive model was plotted, and the area under the curve (AUC) was calculated.Results:The indicators predicting the first remission course included peripheral blood white blood cell count during onset, CBF::MYH11 fusion gene, CEBPA bZIP region mutation, myelodysplastic syndrome-related gene mutation, and induction chemotherapy regimen selection as independent factors for the first remission course. The model’s area under the training and validation curves was 0.738 (95% CI: 0.696-0.780) and 0.726 (95% CI: 0.650-0.801), respectively. The Hosmer-Lemeshow test results yielded P-values of 0.993 and 0.335, respectively. Conclusion:In this study, the developed model demonstrates a strong predictive capability for the efficacy of the first course of patients with AML, providing valuable guidance to clinicians in assessing patient prognosis and selecting appropriate treatment strategies.

Objective:To identify the relevant factors for the first-course remission of acute myeloid leukemia (AML) and to develop a predictive model as well as assess its predictive capability.Methods:Clinical data of 749 patients newly diagnosed with AML admitted to the Department of Hematology, the First Affiliated Hospital, Zhejiang University, School of Medicine from January 1, 2019, to April 30, 2023, were collected and randomly divided into training and validation sets. Multivariate logistic regression analysis was conducted to determine variables associated with complete remission in the first course of induction therapy, and a predictive model was established based on these variables. The receiver operating characteristic (ROC) curve of the predictive model was plotted, and the area under the curve (AUC) was calculated.Results:The indicators predicting the first remission course included peripheral blood white blood cell count during onset, CBF::MYH11 fusion gene, CEBPA bZIP region mutation, myelodysplastic syndrome-related gene mutation, and induction chemotherapy regimen selection as independent factors for the first remission course. The model’s area under the training and validation curves was 0.738 (95% CI: 0.696-0.780) and 0.726 (95% CI: 0.650-0.801), respectively. The Hosmer-Lemeshow test results yielded P-values of 0.993 and 0.335, respectively. Conclusion:In this study, the developed model demonstrates a strong predictive capability for the efficacy of the first course of patients with AML, providing valuable guidance to clinicians in assessing patient prognosis and selecting appropriate treatment strategies.

OBJECTIVE: To prepare customized porous silicone orbital implants using embedded 3D printing and assess the effect of surface modification on the properties of the implants. METHODS: The transparency, fluidity and rheological properties of the supporting media were tested to determine the optimal printing parameters of silicone. The morphological changes of silicone after modification were analyzed by scanning electron microscopy, and the hydrophilicity and hydrophobicity of silicone surface were evaluated by measuring the water contact angle. The compression modulus of porous silicone was measured using compression test. Porcine aortic endothelial cells (PAOECs) were co-cultured with porous silicone scaffolds for 1, 3 and 5 days to test the biocompatibility of silicone. The local inflammatory response to subcutaneous porous silicone implants was evaluated in rats. RESULTS: The optimal printing parameters of silicone orbital implants were determined as the following: supporting medium 4% (mass ratio), printing pressure 1.0 bar and printing speed 6 mm/s. Scanning electron microscopy showed that the silicone surface was successfully modified with polydopamine and collagen, which significantly improved hydrophilicity of the silicone surface (P < 0.05) without causing significant changes in the compression modulus (P > 0.05). The modified porous silicone scaffold had no obvious cytotoxicity and obviously promoted adhesion and proliferation of PAOECs (P < 0.05). In rats bearing the subcutaneous implants, no obvious inflammation was observed in the local tissue. CONCLUSION Poprous silicone orbital implants with uniform pores can be prepared using embedded 3D printing technology, and surface modification obviously improves hydrophilicity and biocompatibility of the silicone implants for potential clinical application.
Animals ; Rats ; Swine ; Silicon ; Orbital Implants ; Endothelial Cells ; Porosity ; Silicones ; Printing, Three-Dimensional

Objective:To evaluate the hemostatic efficacy of N-carboxyethylchitosan fiber gauze (numbered NWL-K) in a leporine bleeding wounds of intraperitoneal parenchymal visceral.Methods:Sixty New Zealand rabbits were divided into two groups according to the randomized digital number method, with 30 rabbits per group. The leporine bleeding models of hepatic or splenic wound were made respectively. The two groups were subdivided into three groups: common gauze group, SURGICEL group and NWL-K group, with 10 rabbits per group. By analyzing the weight of excised liver tissue and amount of bleeding, the model stability was measured. The time to hemostasis and bleeding score in each group were analyzed every (20±5)seconds after compression for 30 seconds in the hepatic bleeding models or every (30±5)seconds after compression for 3 minutes in the splenic bleeding models. The adhesion between wound and gauze was evaluated at the same time.Results:There was no significant difference in the weight of excised liver tissue and amount of bleeding when the hepatic or splenic bleeding models were made ( P>0.05). It showed that the model was made stably and the hemostasis experiment would not be affected. In the splenic wound model experiment, the time to hemostasis was 255(233, 300)seconds in SURGICEL group and 210(180, 248)seconds in NWL-K group, both of which were significantly shorter than 465(383, 660)seconds in common guaze group ( P<0.05). NWL-K achieved shorter time to hemostasis than SURGICEL ( P<0.05). In the hepatic wound model experiment, the time to hemostasis was 90(85, 110)seconds in SURGICEL group and 70(70, 95)seconds in NWL-K group, both of which were significantly shorter than 250(225 290)seconds in common gauze group ( P<0.05). In the splenic wound model experiment, the bleeding score in NWL-K group and SURGICEL group decreased faster than that in common gauze group ( P<0.05). The difference of bleeding score was significant between NWL-K group and SURGICEL group at 180 seconds ( P<0.05). In the hepatic wound model experiment, the bleeding score in NWL-K group and SURGICEL group decreased faster than that in common gauze group at 50 seconds, 70 seconds and 90 seconds ( P<0.05). The bleeding score in common gauze group and NWL-K group showed significant difference at 30 seconds, 110 seconds and 130 seconds ( P<0.05). For the adhesion evaluation, both the water-absorbency and adhesion to tissue of NWL-K were better than common gauze and SURGICEL. Conclusions:For hepatic and splenic bleeding wounds, compared with other types of gauze, the application of NWL-K can effectively shorten the time to hemostasis and reduce the blood loss. The NWL-K shows high water-absorbency and firm adhesion to bleeding wound.

At present, most of the common medical clothes in clinic are uniform medical clothes, but there is no special clothes for patients in intensive care unit (ICU). In recent years, with the extensive application of critical ultrasound in the field of critical medicine, it is obviously difficult to meet the clinical needs on traditional patients' clothes. Guangdong Provincial People's Hospital designed a patient's clothing for critical ultrasound examination. The left/right chest, lateral chest, groin and abdomen of the patient's clothing body were covered with cloth and marked areas. When critically ill patients need to be examined by ultrasound and electrocardiogram, the site can be quickly located only by removing the cloth. At the same time, it can protect patients' privacy, avoid aggravating the condition due to cold, increase patients' comfort in clothing, and also facilitate medical care. It is worthy popularizing in clinic because of its practicability and novel design.

Objective To explore the association of apoB gene polymorphisms with dyslipidemia and lipid levels in Xinjiang Shihezi Han Chinese. Methods 150 dyslipidemia patients and 150 normal pople were involved in this study. EcoRI and XbaI polymorphisms of apolipoprotein B was analyzed by PCR-restriction fragment length polymorphism. The levels of plasma total cholesterol, triglyceride, low density lipoprotein cholesterol, high density lipoprotein cholesterol, apoAI and apoB were determined. Results The frequency of E + E -/E - E - genotype and E-Allele(37.3% and 19% ) in dyslipidemia group was significantly higher than that in controls( 12. 7% and 6. 3% ). The levels of TC, TG and LDL-C in the E +E -/E - E - gene type were significantly higher than those of the E + E + gene type in each group ( P ＜0. 01 ). The frequency of X + X -/X + X + genotype and X + Allele( 20. 7% and 1 1% ) in dyslipidemia group was significantly higher than that in controls (8% and 4% ). The levels ofTC, TG, LDL-C and apoB in the X + X -/X + X + gene type patients were significantly higher than those in the X - X - gene type patients in every group ( P ＜ 0. 05 ). Conclusion The EcoRI and XbaI polymorphism of ApoB gene was related to dyslipidemia in population of Xinjiang Shihezi Han Chinese, and the E - and X + Allele may be the genetic risk factors for dyslipidemia.