Objective To investigate the mechanism of luteolin(LUT)in inhibiting inflammatory aging for treating diabetic retinopathy(DR).Methods Through preliminary experiments,high glucose(HG)and LUT concentrations were established to induce ARPE-19 cells for the experiment.Cells were divided into:Control group(5.5 mmol·L-1 glu-cose),HG group(35 mmol·L-1 glucose),HG+low LUT group(35 mmol·L-1 glucose+20 μmol·L-1 LUT),HG+high LUT group(35 mmol·L-1 glucose+40 μmol·L-1 LUT).Cell viability was measured by CCK-8 assay;Cell migration abil-ity was assessed by scratch assay;ELISA detected inflammatory aging factors[senescence-associated β-galactosidase(SA-β-Gal),interleukin(IL)-1β,IL-6];DCFDA measured intracellular ROS levels;β-galactosidase activity staining identi-fied cellular senescence;TUNEL assay determined apoptosis rate;Western blot and RT-PCR analyzed protein and mRNA expression of silent information regulator 1(SIRT1),nuclear factor(NF)-κB,NOD-like receptor family pyrin domain con-taining 3(NLRP3),and Thioredoxin.Results Compared with the Control group,the cell viability and migration ability of the HG group decreased,and the difference was statistically significant(both P＜0.01);Compared with the HG group,both the HG+low LUT group and the HG+high LUT group showed an increase in cell viability and migration ability,and the differences were statistically significant(both P＜0.05).Compared with the Control group,the expression levels of SA-β-Gal,IL-1 β,and IL-6 in HG group cells were all increased,and the differences were statistically significant(all P＜0.01);Compared with the HG group,the expression levels of SA-β-Gal in the HG+low LUT group and SA-β-Gal,IL-1β,and IL-6 in the HG+high LUT group decreased,and the differences were statistically significant(all P＜0.05).Compared with the Control group,the HG group showed an increase in the number of senescent cells,apoptosis rate,and ROS content in the cells,with statistically significant differences(all P＜0.01);Compared with the HG group,the number of senescent cells,apoptosis rate,and ROS content in the HG+low LUT group and HG+high LUT group were all reduced,and the differences were statistically significant(all P＜0.05).Compared with the Control group,the expression levels of SIRT1 protein and mRNA in HG group cells decreased,while the expression levels of NF-κB,NLRP3,and Thioredoxin protein and mRNA in-creased,and the differences were statistically significant(all P＜0.01);Compared with the HG group,the expression lev-els of SIRT1 protein and mRNA in the HG+low LUT group and HG+high LUT group increased,while the expression levels of NF-κB,NLRP3,and Thioredoxin protein and mRNA decreased,and the differences were statistically significant(all P＜0.05).Conclusion LUT improves cell viability,migration,senescence and apoptosis levels induced by HG while sup-pressing the expression of inflammatory aging factors,which may be related to its regulation of the SIRT1/NF-κB/NLRP3 signaling pathway.

BACKGROUND: The potential impact of pre-existing coronary artery stenosis (CAS) on right ventricular (RV) function during acute pulmonary embolism (PE) episodes remains underexplored. This study aimed to investigate the association between pre-existing CAS and RV dysfunction in patients with acute PE. METHODS: In this multicenter, case-control study, 89 cases and 176 controls matched for age were enrolled at three study centers (Peking Union Medical College Hospital, Fuwai Hospital, and the Second Affiliated Hospital of Harbin Medical University) from January 2016 to December 2020. The cases were patients with acute PE with CAS, and the controls were patients with acute PE without CAS. Coronary artery assessment was performed using coronary computed tomographic angiography. CAS was defined as ≥50% stenosis of the lumen diameter in any coronary vessel >2.0 mm in diameter. Conditional logistic regression analysis was used to evaluate the association between CAS and RV dysfunction. RESULTS: The percentages of RV dysfunction (19.1% [17/89] vs. 44.6% [78/176], P <0.001) and elevated systolic pulmonary artery pressure (sPAP) (19.3% [17/89] vs. 39.5% [68/176], P = 0.001) were significantly lower in the case group than those in the control group. In the multivariable logistic regression model, CAS was independently and negatively associated with RV dysfunction (adjusted odds ratio [OR]: 0.367; 95% confidence interval [CI]: 0.185-0.728; P = 0.004), and elevated sPAP (OR: 0.490; 95% CI: 0.252-0.980; P = 0.035), respectively. CONCLUSIONS Pre-existing CAS was significantly and negatively associated with RV dysfunction and elevated sPAP in patients with acute PE. This finding provides new insights into RV dysfunction in patients with acute PE with pre-existing CAS.
Humans ; Pulmonary Embolism/complications* ; Case-Control Studies ; Male ; Ventricular Dysfunction, Right/physiopathology* ; Female ; Middle Aged ; Aged ; Coronary Stenosis/complications* ; Logistic Models ; Adult

Objective To investigate the mechanism of luteolin(LUT)in inhibiting inflammatory aging for treating diabetic retinopathy(DR).Methods Through preliminary experiments,high glucose(HG)and LUT concentrations were established to induce ARPE-19 cells for the experiment.Cells were divided into:Control group(5.5 mmol·L-1 glu-cose),HG group(35 mmol·L-1 glucose),HG+low LUT group(35 mmol·L-1 glucose+20 μmol·L-1 LUT),HG+high LUT group(35 mmol·L-1 glucose+40 μmol·L-1 LUT).Cell viability was measured by CCK-8 assay;Cell migration abil-ity was assessed by scratch assay;ELISA detected inflammatory aging factors[senescence-associated β-galactosidase(SA-β-Gal),interleukin(IL)-1β,IL-6];DCFDA measured intracellular ROS levels;β-galactosidase activity staining identi-fied cellular senescence;TUNEL assay determined apoptosis rate;Western blot and RT-PCR analyzed protein and mRNA expression of silent information regulator 1(SIRT1),nuclear factor(NF)-κB,NOD-like receptor family pyrin domain con-taining 3(NLRP3),and Thioredoxin.Results Compared with the Control group,the cell viability and migration ability of the HG group decreased,and the difference was statistically significant(both P＜0.01);Compared with the HG group,both the HG+low LUT group and the HG+high LUT group showed an increase in cell viability and migration ability,and the differences were statistically significant(both P＜0.05).Compared with the Control group,the expression levels of SA-β-Gal,IL-1 β,and IL-6 in HG group cells were all increased,and the differences were statistically significant(all P＜0.01);Compared with the HG group,the expression levels of SA-β-Gal in the HG+low LUT group and SA-β-Gal,IL-1β,and IL-6 in the HG+high LUT group decreased,and the differences were statistically significant(all P＜0.05).Compared with the Control group,the HG group showed an increase in the number of senescent cells,apoptosis rate,and ROS content in the cells,with statistically significant differences(all P＜0.01);Compared with the HG group,the number of senescent cells,apoptosis rate,and ROS content in the HG+low LUT group and HG+high LUT group were all reduced,and the differences were statistically significant(all P＜0.05).Compared with the Control group,the expression levels of SIRT1 protein and mRNA in HG group cells decreased,while the expression levels of NF-κB,NLRP3,and Thioredoxin protein and mRNA in-creased,and the differences were statistically significant(all P＜0.01);Compared with the HG group,the expression lev-els of SIRT1 protein and mRNA in the HG+low LUT group and HG+high LUT group increased,while the expression levels of NF-κB,NLRP3,and Thioredoxin protein and mRNA decreased,and the differences were statistically significant(all P＜0.05).Conclusion LUT improves cell viability,migration,senescence and apoptosis levels induced by HG while sup-pressing the expression of inflammatory aging factors,which may be related to its regulation of the SIRT1/NF-κB/NLRP3 signaling pathway.

Background::The potential impact of pre-existing coronary artery stenosis (CAS) on acute pulmonary embolism (PE) episodes remains underexplored. This study aimed to investigate the association between pre-existing CAS and the elevation of high-sensitivity cardiac troponin I (hs-cTnI) levels in patients with PE.Methods::In this multicenter, prospective case-control study, 88 cases and 163 controls matched for age, sex, and study center were enrolled. Cases were patients with PE with elevated hs-cTnI. Controls were patients with PE with normal hs-cTnI. Coronary artery assessment utilized coronary computed tomographic angiography or invasive coronary angiography. CAS was defined as ≥50% stenosis of the lumen diameter in any coronary vessel >2.0 mm in diameter. Conditional logistic regression was used to evaluate the association between CAS and hs-cTnI elevation.Results::The percentage of CAS was higher in the case group compared to the control group (44.3% [39/88] vs. 30.1% [49/163]; P = 0.024). In multivariable conditional logistic regression model 1, CAS (adjusted odds ratio [OR], 2.680; 95% confidence interval [CI], 1.243–5.779), heart rate >75 beats/min (OR, 2.306; 95% CI, 1.056–5.036) and N-terminal pro-B type natriuretic peptide (NT-proBNP) >420 pg/mL (OR, 12.169; 95% CI, 4.792–30.900) were independently associated with elevated hs-cTnI. In model 2, right CAS (OR, 3.615; 95% CI, 1.467–8.909) and NT-proBNP >420 pg/mL (OR, 13.890; 95% CI, 5.288–36.484) were independently associated with elevated hs-cTnI. Conclusions::CAS was independently associated with myocardial injury in patients with PE. Vigilance towards CAS is warranted in patients with PE with elevated cardiac troponin levels.

Objective::Cardiac damage is commonly reported in patients with coronavirus disease 2019 (COVID-19) but its prevalence and impact on the long-term survival of patients remain uncertain. This study aimed to explore the prevalence of myocardial injury and assess its prognostic value in patients with COVID-19.Methods::A single-center, retrospective cohort study was performed at the Affiliated Hospital of Jianghan University. Data from 766 patients with confirmed COVID-19 who were hospitalized from December 27, 2019 to April 25, 2020 were collected. Demographic, clinical, laboratory, electrocardiogram, treatment data and all-cause mortality during follow-up were collected and analyzed.Results::Of the 766 patients with moderate to critically ill COVID-19, 86 (11.2%) died after a mean follow-up of 72.8 days. Myocardial injury occurred in 94 (12.3%) patients. The mortality rate was 64.9% (61/94) and 3.7% (25/672) in patients with and without myocardial injury, respectively. Cox regression showed that myocardial injury was an independent risk factor for mortality (hazard ratio: 8.76, 95% confidence interval: 4.76-16.11, P < 0.001). Of the 90 patients with myocardial injury with electrocardiogram results, sinus tachycardia was present in 29, bundle branch block in 26, low voltage in 10, and abnormal T-wave in 53. Conclusions::COVID-19 not only involves pneumonia but also cardiac damage. Myocardial injury is a common complication and an independent risk factor for mortality in COVID-19 patients.

Objective::Cardiac damage is commonly reported in patients with coronavirus disease 2019 (COVID-19) but its prevalence and impact on the long-term survival of patients remain uncertain. This study aimed to explore the prevalence of myocardial injury and assess its prognostic value in patients with COVID-19.Methods::A single-center, retrospective cohort study was performed at the Affiliated Hospital of Jianghan University. Data from 766 patients with confirmed COVID-19 who were hospitalized from December 27, 2019 to April 25, 2020 were collected. Demographic, clinical, laboratory, electrocardiogram, treatment data and all-cause mortality during follow-up were collected and analyzed.Results::Of the 766 patients with moderate to critically ill COVID-19, 86 (11.2%) died after a mean follow-up of 72.8 days. Myocardial injury occurred in 94 (12.3%) patients. The mortality rate was 64.9% (61/94) and 3.7% (25/672) in patients with and without myocardial injury, respectively. Cox regression showed that myocardial injury was an independent risk factor for mortality (hazard ratio: 8.76, 95% confidence interval: 4.76-16.11, P < 0.001). Of the 90 patients with myocardial injury with electrocardiogram results, sinus tachycardia was present in 29, bundle branch block in 26, low voltage in 10, and abnormal T-wave in 53. Conclusions::COVID-19 not only involves pneumonia but also cardiac damage. Myocardial injury is a common complication and an independent risk factor for mortality in COVID-19 patients.

OBJECTIVE: To study the change of endogenous hydrogen sulfide (hydrogen sulfide, H2S) and its rate-limiting enzyme Cystathionine-gamma-lyase (CSE) in allergic rhinitis through guinea pigs with intervention treatment. METHOD: Twenty-four guinea pigs were divide into 4 groups at random, one group were models of allergic rhinitis (AR) which were established by using ovalbumin, the second group were treated with NaHS after sensitized, the third group were treated with Propargylglycine (PPG) which was suppression of CSE after sensitized, and the last group were treated with saline for control. The concentration of eotaxin of nasal lavage and H2S in plasma were recorded, and then the expression of CSE in nasal mucosa was determined by real-time fluorescence RT-PCR. RESULT: The concentration of eotaxin in nasal lavage of sensitized group were higher than those of control (P < 0.01), and concentration of H2S in plasma and expression of CSE in nasal mucosa were lower than control (P < 0.05). The concentration of eotaxin decreased when treated with NaHS and increased when treated with PGG (P < 0.05). Level of H2S in plasma and expression of CSE increased when treated with NaHS and decreased when treated with PGG (P < 0.05), and the level of H2S was positive linear correlate with the expression of CSE. CONCLUSION Endogenous H2S perhaps plays a significant role in the pathogenesis of allergic rhinitis, and it was mainly regulated by CSE.
Animals ; Cystathionine gamma-Lyase ; metabolism ; Guinea Pigs ; Hydrogen Sulfide ; metabolism ; Male ; Nasal Mucosa ; metabolism ; Rhinitis ; metabolism