Author:(Yinhao HUANG)
1.Acteoside ameliorates hepatocyte ferroptosis and hepatic ischemia-reperfusion injury via targeting PCBP2.
Kexin JIA ; Yinhao ZHANG ; Fanghong LI ; Runping LIU ; Jianzhi WU ; Jiaorong QU ; Ranyi LUO ; Zixi HUANG ; Zhe XU ; Xiaojiaoyang LI
Acta Pharmaceutica Sinica B 2025;15(4):2077-2094
Hepatic ischemia-reperfusion injury (HIRI) has been considered as an inevitable process of liver transplantation. Hepatocyte ferroptosis is a key factor in HIRI development, yet precise mechanism and potential therapies are still unclear. Here, we demonstrated a strong correlation between hepatocyte ferroptosis and the downregulation of poly(rC)-binding protein (PCBP2), which compromised the stability of antiporter system Xc- (consisted of SL3A2/SLC7A11). Besides, inhibiting PCBP2 contributed to facilitating cofactor p300 to enhance the transcriptional activity of HIF1α, leading to the expression and secretion of HMGB1. Then, released HMGB1 from ferroptotic hepatocytes worsened M1 macrophage recruitment and immune response during HIRI. Additionally, acteoside (ACT) was shown to assist PCBP2 in stabilizing the mRNA stability of Slc3a2 and Slc7a11, as well as enhance the binding affinity of PCBP2-system Xc-. Beyond that, ACT also supported PCBP2 to limit HMGB1-induced M1 macrophage recruitment through imposing restrictions on p300 and HIF1α. Furthermore, specific knockdown of PCBP2 in hepatocytes directly interposed the therapeutic efficacy of ACT on HIRI mice. In conclusion, ACT alleviated hepatocyte ferroptosis and HIRI via promoting PCBP2 to maintain the stability of system Xc- and limit HIF1α/p300-HMGB1 signaling. These findings highlight the therapeutic benefits of ACT in treating HIRI and offer insights into innovative therapeutic strategies.
2.Anthraquinones of Cassiae Semen alleviate lipid accumulation in obesity by regulating brown adipose tissue and liver function.
Yijie LI ; Ruiyu WU ; Xin LI ; Jianan LI ; Yinhao ZHANG ; Yanbo HUANG ; Guifang FAN ; Xiaojiaoyang LI
Chinese Herbal Medicines 2025;17(3):488-499
OBJECTIVE:
Cassiae Semen (CS, Juemingzi in Chinese) is a widely used traditional Chinese medicine with a variety of pharmacological effects. This study aimed to investigate the potential therapeutic effects and molecular mechanisms of anthraquinones of CS (AQS) for adiposity.
METHODS:
The chemical components of the AQS were determined using high-performance liquid chromatography (HPLC). Network pharmacology analysis was used to predict potential anti-obesity targets of action for AQS. We constructed high fat with high sugar water diet-induced obese mice and observed their body weight and whole-body lipid metabolism to evaluate the efficacy of AQS in promoting lipid metabolism. Subsequently, the epidermal temperature at the brown adipose tissue (BAT) before and after cold stimulation was observed and the expression of lipid metabolism-related genes in the liver and BAT tissues was detected to clarify the mechanism of action of AQS.
RESULTS:
Network pharmacology analysis showed that AQS was involved in the regulation of liver and adipose tissue function under obesity. Pathological and biochemical results showed that AQS reduced lipid accumulation in the liver and adipose tissue induced by an unhealthy diet. With the increase of cold tolerance, the volume and weight of BAT were increased by AQS, suggesting that it regulated the body heat production dominated by BAT. After AQS treatment, the levels of genes related to uncoupling protein1 (UCP1)-mediated adaptive thermogenesis in BAT tissues and lipid metabolism in the liver were also increased, which further proved that AQS activated BAT function to promote lipid metabolism in the whole body.
CONCLUSION
This study revealed the pharmacological effects of AQS, thereby providing a scientific basis for regulating BAT thermogenesis and liver lipid metabolism to alleviate obesity and providing clues for further exploring the application of natural active ingredients in the treatment of metabolism-related diseases.
3.Analysis of media information monitoring results of COVID-19 in Shanghai in 2022 based on an internet surveillance system
Yinhao LU ; Jin WANG ; Fang ZHANG ; Fangfang TAO ; Xiaoyan HUANG
Shanghai Journal of Preventive Medicine 2023;35(7):644-649
ObjectiveTo understand the internet media information of COVID-19 in Shanghai in 2022， to provide evidence for health administration departments to respond to public health emergencies， and to establish a comprehensive public health media surveillance system. MethodsData on internet media information and the local COVID-19 epidemic data in Shanghai from January 1 to June 30， 2022 were collected and analyzed. ResultsA total of 90.197 3 million pieces of internet media information were monitored. The top four sources of the information were Weibo （36.84%）， short videos （26.19%）， internet media （14.47%）， and forums （13.31%）. The variation trend of daily internet media surveillance information was identified with the number of newly reported cases per day， and a correlation was found （r＝0.770. After the seven-day moving average，r＝0.796）. Frequent keywords included Shanghai， cheer up， nucleic acid， new cases， local， asymptomatic， prevention and control， gratitude， isolation， epidemic prevention， supplies， pandemic response， patient， seeking help， and clearing the cases. Within the information on Weibo， in addition to netizens in Shanghai （7 823 100） who were highly concerned about the pandemic， netizens from Beijing （2 749 100）， Guangdong Province （2 352 300）， Jiangsu Province （2 189 100） and Zhejiang Province（1 532 100） also posted a relatively high amount of information related to COVID-19 in Shanghai. ConclusionThe outbreak of major infectious diseases triggers significant online activity， and platforms such as Weibo and short video platforms play crucial roles in disseminating internet media information. Weibo， short videos etc. are important platforms for current internet media information. Strengthening media information monitoring and analysis， paying close attention to hot events， taking timely response measures and actively engaging in risk communication contribute to the response and management of public health emergencies.
4.Investigation of public health emergency core capacity of district-level disease prevention and control institutions under normalized epidemic prevention and control
Wenjun WU ; Fang XU ; Han WU ; Rong CHEN ; Yinhao LU ; Xiaoyan HUANG
Shanghai Journal of Preventive Medicine 2022;34(4):303-308
ObjectiveBased on the investigation of the core capacity development of health emergency response of Shanghai disease prevention and control institutions after the COVID-19 pandemic， to analyze the shortcomings of health emergency response capacity of Shanghai disease prevention and control institutions， and to put forward suggestions to improve the core capacity of Shanghai's disease prevention and control system in the face of public health emergencies. MethodsUsing questionnaire surveys， investigations and evaluations were conducted on the emergency system development， epidemic monitoring and risk assessment， emergency team manpower and equipment， and actual response to COVID-19 in 16 district-level disease prevention and control institutions in Shanghai. ResultsThe overall public health emergency response capacity building of the district-level disease control agencies in Shanghai is relatively good， and the effect of capacity building has been proven during the handling of the new coronavirus. But at the same time， it also exposed some specific problems such as imperfect system construction， lack of emergency personnel and materials， insufficient on-site handling capabilities of personnel， and uneven allocation of emergency personnel and materials among different agencies. ConclusionShanghai district-level disease control should focus on the specific problems， such as strengthening the development of health emergency system， improving the human resources and material reserves of the emergency teams， and optimizing the laboratory testing ability， to further improve the core health emergency capacity of Shanghai disease control system.
5.Epidemiological analysis of registered tuberculosis cases in Kashgar District， Xinjiang Uygur Autonomous Region from 2011 to 2020
Tusun DIERMULATI ; Xiaoyan HUANG ; Abulimiti MAIWEILANJIANG ; Yimamu MAIWULAJIANG ; Xiaowang PENG ; Abudureyimu TUERHONG ; Yinhao LU ; Yi HE
Shanghai Journal of Preventive Medicine 2022;34(11):1090-1095
ObjectiveTo determine the current status and characteristics of tuberculosis （TB） registration and treatment in Kashgar， and to provide scientific evidence for targeted prevention and control measures in future. MethodsKashgar registered TB cases information in 2011 to 2020 was exported from the National Tuberculosis Management Information System. Descriptive epidemiological analysis was conducted using Stata 12.0. ResultsFrom 2011 to 2020， number of Kashgar registered TB patients showed rising trend， followed by a falling one. Average proportion of annual decline in registered TB incidence was 40.48% from 2018 to 2020. From 2011 to 2016， number of registered TB patients in women was always higher than that in men， with a gender ratio （male ： female） of about 0.90. In 2017， the gender ratio was 1.00. From 2018 to 2020， the gender ratios were 1.05， 1.20， and 1.12， respectively. Moreover， number of registered TB cases increased with age （χ2=547.79， P<0.001）. Proportion of registered TB cases was relatively large in Shache County （16.43%‒23.64%）， Yengisar County （9.51%‒13.87%） ， Kashgar City （8.11%‒11.40%）， Yecheng County （6.98%‒13.40%） and Bachu County（4.92%‒16.65%）. Proportion of recurrent TB cases in Kashgar had increased to 27.29%， 20.77% and 28.39% in 2018， 2019 and 2020， respectively. Multivariate analysis showed that age， drug resistance， calendar year and etiological diagnosis were significantly correlated with the proportion of recurrent cases （all P<0.05）. ConclusionSince 2018， TB incidence has decreased significantly due to the increasing efforts for identification and treatment of TB cases. However， Kashgar remains facing a high TB incidence. TB cases that are elderly， drug-resistant and positive for pathogen are susceptible to recurrent treatment. In future， targeted prevention and control measures should be improved for these groups.
6.Analysis on the surveillance of infectious disease related public health bud-events in Shanghai from 2017 to 2020
Yinhao LU ; Yongchao HE ; Yi HE ; Huanyu WU ; Chunyan LUO ; Xiaoyan HUANG
Shanghai Journal of Preventive Medicine 2022;34(1):17-21
Objective To determine the epidemiological characteristics of infectious disease related public health bud-events in Shanghai and assess the effects of bud-event surveillance， so as to provide scientific evidence for improving the surveillance system. Methods Surveillance data of infectious disease related public health bud-events were collected from 16 districts of Shanghai from 2017 through 2020. Then the data were analyzed and compared with infectious disease related public health emergencies during the same period. Results A total of 6 376 infectious disease related public health bud-events were documented in Shanghai in 2017‒2020， which involved 29 792 cases. There were two seasonal peaks， April through June and November through December. Clustered events accounted for 38.85%， mainly caused by chickenpox （14.10%）， hand，foot and mouth disease （11.17%） and norovirus-associated infectious diarrhea （6.54%）. The 36.73% of the bud-events occurred in school settings， which involved 24 718 cases （accounting for 83.00% of all cases）. Median time duration between onset date of the first cases and report date of the events was 4 days， and median duration of the events was 14 days， demonstrating positive correlation. In addition， all the infectious disease related public health emergencies（
7.Resection of giant neurofibroma in a case of neurofibromatosis type 1 followed by replantation of the skin from tumor surface
Hang LONG ; Jiali XU ; Yinhao HUANG ; Zhu ZHU ; Jian TIAN ; Yubo WANG ; Xiaolin ZHANG
Chinese Journal of Plastic Surgery 2020;36(5):568-570
Neurofibromatosis type 1 combined with giant neurofibroma is very rare, and treatment is quite difficult. October 2019, a 23 year-old female patient of neurofibromatosis type 1 combined with giant neurofibroma at the shoulder and back was treated at the Department of Burn and Plastic surgery, the First People’s Hospital of Bijie Municipal. The giant neurofibroma was resected and the skin from the tumor surface was replanted to cover the wound. During the following period of 3 months, satisfactory results were achieved for both donor site and recipient site.
8.Resection of giant neurofibroma in a case of neurofibromatosis type 1 followed by replantation of the skin from tumor surface
Hang LONG ; Jiali XU ; Yinhao HUANG ; Zhu ZHU ; Jian TIAN ; Yubo WANG ; Xiaolin ZHANG
Chinese Journal of Plastic Surgery 2020;36(5):568-570
Neurofibromatosis type 1 combined with giant neurofibroma is very rare, and treatment is quite difficult. October 2019, a 23 year-old female patient of neurofibromatosis type 1 combined with giant neurofibroma at the shoulder and back was treated at the Department of Burn and Plastic surgery, the First People’s Hospital of Bijie Municipal. The giant neurofibroma was resected and the skin from the tumor surface was replanted to cover the wound. During the following period of 3 months, satisfactory results were achieved for both donor site and recipient site.
9.Epidemiological analysis on bud-events of Norovirus-associated infectious diarrhea in Shanghai, 2018
Yi HE ; Yinhao LU ; Xiaoyan HUANG ; Wenjia XIAO ; Fangfang TAO ; Huanyu WU ; Chunyan LUO ; Zhengan YUAN
Chinese Journal of Epidemiology 2020;41(4):547-551
Objective:To analyze the epidemiological characteristics related to Norovirus-associated infectious diarrhea bud events in Shanghai, and to discuss the value of bud events surveillance, so as to help Norovirus-associated infectious diarrhea control, prevention and to improve the surveillance system on bud events.Methods:Data related to 142 Norovirus-associated infectious diarrhea bud events were gathered from 16 districts of Shanghai areas in 2018 and were analyzed.Results:There were 2 peaks, April and November for 142 Norovirus-associated infectious diarrhea bud events reported in 2018. 98.59 % (140/142) of the bud events occurred in schools and kindergartens. 80.28 % (114/142) of the bud events would involve 6-19 cases per each event. The median time duration between the onset date of the first case and the reporting date of the event was 2 days and the median event duration was 7 days. The bud event scale and regional incidence of bud events as well as the reporting timelines were influential factors on the duration of the bud events. Conclusions:Two peaks, spring and winter bud events of the Norovirus-associated infectious diarrhea were seen in Shanghai in 2018. Schools and kindergartens were to be prioritized locations for control and prevention of Norovirus-associated infectious diarrhea bud events. Surveillance system was proved to have contributed to the early detection, reporting and control of bud events.
10. Correlation between multi-slice spiral CT determined epicardial adipose tissue volume and atrial fibrillation
Yanmin ZHU ; Haixia XU ; Qi LU ; Yinhao HUANG ; Hongmei JING ; Xiang WU
Chinese Journal of Cardiology 2019;47(12):969-973
Objective:
To determine the relationship between volume of epicardial adipose tissue (EAT) and atrial fibrillation (AF) .
Methods:
A total of 207 patients who hospitalized in the Department of Cardiology, Nantong University Affiliated Hospital from January 2016 to June 2018 were included in this study. They were divided into two groups, including AF group (
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