Objective To investigate the expressions of long non-coding ribonucleic acid OPA-interacting protein 5 antisense transcript 1(lncRNA OIP5-AS1)and microribonucleic acid 22-3p(miR-22-3p)in gastric cancer tissue and their relationships with clinicopathological features and prognosis.Methods A total of 106 patients diagnosed with gastric cancer in Zhuji Central Hospital from June 2017 to November 2020 were retrospectively enrolled as the study subjects.Cancer tissue and adjacent tissue(＞5cm from the lesion)were collected during surgery and assigned into the gastric cancer group and adjacent group,respectively.Quantitative reverse transcription polymerase chain reaction(qRT-PCR)was applied to detect the levels of lncRNA OIP5-AS1 and miR-22-3p in various tissues.The prognoses were poor(death)in 40 patients(poor prognosis group)and good in 66 patients(good prognosis group).Pearson method was applied to analyze the relationship between lncRNA OIP5-AS1 and miR-22-3p levels in cancer tissues.Kaplan-Meier method was applied to analyze the relationships of the expression levels of lncRNA OIP5-AS1 and miR-22-3p with prognosis.COX re-gression analysis was performed to analyze the factors influencing the prognosis.Results Compared with the adjacent group,the gastric cancer group had significantly increased lncRNA OIP5-AS1 level and significantly reduced miR-22-3p level(all P＜0.05).Lucifer-ase assay showed that lncRNA OIP5-AS1had a targeting relationship with miR-22-3p.The expression level of lncRNA OIP5-AS1 was negatively correlated with that of miR-22-3p in cancer tissues(rp=-0.426,P＜0.001).The expression levels of lncRNA OIP5-AS1 and miR-22-3p were closely related to tumor staging and differentiation(all P＜0.05).Compared with the good prognosis group,the poor prognosis group had significantly increased lncRNA OIP5-AS1 level and significantly reduced miR-22-3p level in cancer tissues(all P＜0.05).Kaplan-Meier method reveled that the 3-year survival rate was significantly lower in the high lncRNA OIP5-AS1 expression group than in the low lncRNA OIP5-AS1 expression group(x2=27.667,P＜0.001)but was significantly higher in the high miR-22-3p expression group than in the low miR-22-3p expression group(x2=19.876,P＜0.001).COX regression showed that the expressions of lncRNA OIP5-AS1(HR=3.652)and miR-22-3p(HR=0.748)were the prognostic factors for gas-tric cancer patients(all P＜0.05).Conclusion LncRNA OIP5-AS1 is highly expressed in gstric cancer tissues,while miR-22-3p is lowly expressed.The two are negatively correlated,and both of them are closely related to the clinicopathological features and prognosis of gastric cancer.

Objective To investigate the expressions of long non-coding ribonucleic acid OPA-interacting protein 5 antisense transcript 1(lncRNA OIP5-AS1)and microribonucleic acid 22-3p(miR-22-3p)in gastric cancer tissue and their relationships with clinicopathological features and prognosis.Methods A total of 106 patients diagnosed with gastric cancer in Zhuji Central Hospital from June 2017 to November 2020 were retrospectively enrolled as the study subjects.Cancer tissue and adjacent tissue(＞5cm from the lesion)were collected during surgery and assigned into the gastric cancer group and adjacent group,respectively.Quantitative reverse transcription polymerase chain reaction(qRT-PCR)was applied to detect the levels of lncRNA OIP5-AS1 and miR-22-3p in various tissues.The prognoses were poor(death)in 40 patients(poor prognosis group)and good in 66 patients(good prognosis group).Pearson method was applied to analyze the relationship between lncRNA OIP5-AS1 and miR-22-3p levels in cancer tissues.Kaplan-Meier method was applied to analyze the relationships of the expression levels of lncRNA OIP5-AS1 and miR-22-3p with prognosis.COX re-gression analysis was performed to analyze the factors influencing the prognosis.Results Compared with the adjacent group,the gastric cancer group had significantly increased lncRNA OIP5-AS1 level and significantly reduced miR-22-3p level(all P＜0.05).Lucifer-ase assay showed that lncRNA OIP5-AS1had a targeting relationship with miR-22-3p.The expression level of lncRNA OIP5-AS1 was negatively correlated with that of miR-22-3p in cancer tissues(rp=-0.426,P＜0.001).The expression levels of lncRNA OIP5-AS1 and miR-22-3p were closely related to tumor staging and differentiation(all P＜0.05).Compared with the good prognosis group,the poor prognosis group had significantly increased lncRNA OIP5-AS1 level and significantly reduced miR-22-3p level in cancer tissues(all P＜0.05).Kaplan-Meier method reveled that the 3-year survival rate was significantly lower in the high lncRNA OIP5-AS1 expression group than in the low lncRNA OIP5-AS1 expression group(x2=27.667,P＜0.001)but was significantly higher in the high miR-22-3p expression group than in the low miR-22-3p expression group(x2=19.876,P＜0.001).COX regression showed that the expressions of lncRNA OIP5-AS1(HR=3.652)and miR-22-3p(HR=0.748)were the prognostic factors for gas-tric cancer patients(all P＜0.05).Conclusion LncRNA OIP5-AS1 is highly expressed in gstric cancer tissues,while miR-22-3p is lowly expressed.The two are negatively correlated,and both of them are closely related to the clinicopathological features and prognosis of gastric cancer.