Objective:To explore the impact of severe ovarian hyperstimulation syndrome (OHSS) after fresh embryo transfer on the maternal and neonatal outcomes of patients.Methods:A respective cohort study was performed. Totally 155 patients with severe OHSS (OHSS group) treated in the Department of Obstetrics and Gynecology of Chongqing Health Center for Women and Children and received fresh embryo transfer in the Reproductive Center from January 2018 to December 2018 were selected, and 366 clinical pregnant patients without OHSS after transplantation served as control group (non-OHSS group). According to Mathur's OHSS time classification standard, OHSS patients were divided into early-onset subgroup, occurring within 9 d (including 9 d) after oocytes retrieved, and late-onset subgroup, occurring within 10 d or more after oocytes retrieved; according to the number of pregnancies, patients were divided into singleton subgroup and twin subgroup. The differences in maternal and neonatal outcomes (primary indicators) and clinical indicators (secondary indicators) were compared between the two groups.Results:In OHSS group, 34 early-onset and 121 late-onset, 80 singletons and 75 twins were included and in non-OHSS group 194 singletons and 172 twins were included. Compared with non-OHSS group, patients in OHSS group had lower maternal age [(29.99±4.02) years vs. (31.85±3.62) years, P<0.001] and bigger number of oocytes retrieved (11.62±4.17 vs. 9.48±4.39, P<0.001). For OHSS patients, compared with late-onset group, the ovarian size [(7.95±1.46) cm vs. (7.26±1.41) cm], depth of chest water [(5.83±4.57) cm vs. (3.69±4.20) cm], hematocrit (HCT) [(44.59±4.85)% vs. (42.03±4.53)%], D-dimer [(2.87±1.84) mg/L FEU vs. (2.01±1.09) mg/L FEU], percentage of patients undergoing abdominal puncture and hospitalization days [(12.91±6.64) d vs. (10.12±6.18) d] were higher in early-onset group ( P=0.013, P=0.012, P=0.005, P<0.001, P=0.023, P=0.024), while the number of oocytes retrieved, serum albumin (ALB), level of liver enzyme and percentage of patients undergoing pleural puncture were not significantly different between the two groups (all P>0.05). The clinical data had no significant differences between singleton subgroup and twin subgroup (all P>0.05). The twins rate, the miscarriage rate, the ectopic pregnancy rate and the live birth rate were not significantly different between OHSS group and non-OHSS group (all P>0.05). There were no statistically significant differences in twin rate, live birth rate, obstetric complications, cesarean section rate, postpartum hemorrhage rate or neonatal outcomes between the early and late OHSS patients (all P>0.05). Whether in singleton pregnancy or twin pregnancy, the obstetric complications, preterm birth rate, postpartum bleeding rate and neonatal outcome were not significantly different between OHSS group and non-OHSS group (all P>0.05). Whether in OHSS or non-OHSS patients, the preterm birth rate [54.46% (116/213)], the cesarean section rate [96.71% (206/213)] and the low birth weight rate [59.39% (253/426)] were higher in twin subgroup than in singleton subgroup [11.60% (29/250), P<0.001; 74.00% (185/250), P<0.001; 5.20% (13/250), P<0.001]. Conclusion:The presentation and onset time of severe OHSS after fresh embryo transplantation had no adverse effect on the long-term pregnancy complications and pregnancy outcomes. Early-onset severe OHSS increased adverse effects on maternal in early pregnancy. Multiple pregnancies significantly increase the neonatal adverse outcomes.

Objective:To explore the impact of severe ovarian hyperstimulation syndrome (OHSS) after fresh embryo transfer on the maternal and neonatal outcomes of patients.Methods:A respective cohort study was performed. Totally 155 patients with severe OHSS (OHSS group) treated in the Department of Obstetrics and Gynecology of Chongqing Health Center for Women and Children and received fresh embryo transfer in the Reproductive Center from January 2018 to December 2018 were selected, and 366 clinical pregnant patients without OHSS after transplantation served as control group (non-OHSS group). According to Mathur's OHSS time classification standard, OHSS patients were divided into early-onset subgroup, occurring within 9 d (including 9 d) after oocytes retrieved, and late-onset subgroup, occurring within 10 d or more after oocytes retrieved; according to the number of pregnancies, patients were divided into singleton subgroup and twin subgroup. The differences in maternal and neonatal outcomes (primary indicators) and clinical indicators (secondary indicators) were compared between the two groups.Results:In OHSS group, 34 early-onset and 121 late-onset, 80 singletons and 75 twins were included and in non-OHSS group 194 singletons and 172 twins were included. Compared with non-OHSS group, patients in OHSS group had lower maternal age [(29.99±4.02) years vs. (31.85±3.62) years, P<0.001] and bigger number of oocytes retrieved (11.62±4.17 vs. 9.48±4.39, P<0.001). For OHSS patients, compared with late-onset group, the ovarian size [(7.95±1.46) cm vs. (7.26±1.41) cm], depth of chest water [(5.83±4.57) cm vs. (3.69±4.20) cm], hematocrit (HCT) [(44.59±4.85)% vs. (42.03±4.53)%], D-dimer [(2.87±1.84) mg/L FEU vs. (2.01±1.09) mg/L FEU], percentage of patients undergoing abdominal puncture and hospitalization days [(12.91±6.64) d vs. (10.12±6.18) d] were higher in early-onset group ( P=0.013, P=0.012, P=0.005, P<0.001, P=0.023, P=0.024), while the number of oocytes retrieved, serum albumin (ALB), level of liver enzyme and percentage of patients undergoing pleural puncture were not significantly different between the two groups (all P>0.05). The clinical data had no significant differences between singleton subgroup and twin subgroup (all P>0.05). The twins rate, the miscarriage rate, the ectopic pregnancy rate and the live birth rate were not significantly different between OHSS group and non-OHSS group (all P>0.05). There were no statistically significant differences in twin rate, live birth rate, obstetric complications, cesarean section rate, postpartum hemorrhage rate or neonatal outcomes between the early and late OHSS patients (all P>0.05). Whether in singleton pregnancy or twin pregnancy, the obstetric complications, preterm birth rate, postpartum bleeding rate and neonatal outcome were not significantly different between OHSS group and non-OHSS group (all P>0.05). Whether in OHSS or non-OHSS patients, the preterm birth rate [54.46% (116/213)], the cesarean section rate [96.71% (206/213)] and the low birth weight rate [59.39% (253/426)] were higher in twin subgroup than in singleton subgroup [11.60% (29/250), P<0.001; 74.00% (185/250), P<0.001; 5.20% (13/250), P<0.001]. Conclusion:The presentation and onset time of severe OHSS after fresh embryo transplantation had no adverse effect on the long-term pregnancy complications and pregnancy outcomes. Early-onset severe OHSS increased adverse effects on maternal in early pregnancy. Multiple pregnancies significantly increase the neonatal adverse outcomes.

Objective:To analyze the high risk factors of intramural ectopic pregnancy, and to improve the clinical diagnosis and treatment of intramural ectopic pregnancy.Methods:From January 2015 to July 2019, the clinical data of 9 patients with intramural pregnancy in Chongqing Maternal and Child Health Hospital were retrospectively analyzed. The high risk factors, clinical manifestations, treatment outcomes of intramural pregnancy were summarized.Results:Nine patients with intramural pregnancy, 6 cases had previous history of abortion, uterine or abdominal cavity surgery. Five cases had history of embryo transfer. The uterine integrity was preserved in 9 patients after treatment with medication, hysteroscopy, laparoscopy or transabdominal intramural pregnancy clearance. Pregnancy continued after laparoscopic treatment in a patient with early pregnancy complicated with intramural pregnancy.Conclusion:Patients with high risk factors should be treated at the early stage of pregnancy. Hysteroscopy combined with laparoscopic exploration is an effective method for the treatment of intramural pregnancy, which can make a quick and definite diagnosis and perform minimally invasive surgery, and preserve uterine integrity and fertility.

Objective:To analyze the high risk factors of intramural ectopic pregnancy, and to improve the clinical diagnosis and treatment of intramural ectopic pregnancy.Methods:From January 2015 to July 2019, the clinical data of 9 patients with intramural pregnancy in Chongqing Maternal and Child Health Hospital were retrospectively analyzed. The high risk factors, clinical manifestations, treatment outcomes of intramural pregnancy were summarized.Results:Nine patients with intramural pregnancy, 6 cases had previous history of abortion, uterine or abdominal cavity surgery. Five cases had history of embryo transfer. The uterine integrity was preserved in 9 patients after treatment with medication, hysteroscopy, laparoscopy or transabdominal intramural pregnancy clearance. Pregnancy continued after laparoscopic treatment in a patient with early pregnancy complicated with intramural pregnancy.Conclusion:Patients with high risk factors should be treated at the early stage of pregnancy. Hysteroscopy combined with laparoscopic exploration is an effective method for the treatment of intramural pregnancy, which can make a quick and definite diagnosis and perform minimally invasive surgery, and preserve uterine integrity and fertility.

Objective: To explore the association between dust exposure and the incidence of hypertension in male coal miners. Methods: Using the method of retrospective cohort study,a hypertension cohort of colliery in Henan Province was established in January 2006. From 2006 to 2017,all the male coal miners in a colliery who were exposed to dust were selected into the exposure group including tunneling, mining,auxiliary and combining workers, and workers from administrative logistics departments who were not exposed to dust were selected into the control group. The eligible participants should satisfy following conditions: working more than one year, with clear and complete record of occupation change, and with complete archives and reliable diagnosis of occupational health surveillance. The exclusion criteria of participants were with hypertension at the baseline of study or with heart,liver,kidney diseases and malignant tumors. A total of 12 647 participants were enrolled in this study (11 663 in the exposure group and 984 in the control group). The follow-up period was from January 2006 to December 2017,with a total follow-up of 89 259.75 person-years. Questionnaires and physical measurements were used to collect general demographic characteristics, occupational exposure history and occupational health surveillance data of all participants. The Cox proportional hazards regression model was used to estimate the association between the dust exposure and the incidence of hypertension. Results: During the follow-up period, 2 549 new-onset hypertension patients were identified with an incidence density of hypertension about 2 855.71 per 100 000 person-years. The incidence density of hypertension was 2 967.58 per 100 000 person-years in the exposure group, and 1 643.85 per 100 000 person-years in the control group. The results of multivariate Cox proportional hazards regression model showed that after the adjustment of marriage, age, smoking, alcohol drinking and body mass index,the risk of hypertension was higher in the exposure group compared with the control group (HR=1.692, 95%CI: 1.410-2.032). Further analysis showed that compared with workers from administrative logistics departments,the risk of hypertension in tunneling,mining and auxiliary working was 1.629(1.345-1.973),1.677(1.374-2.046) and 1.782(1.475-2.151),respectively. Conclusion Dust exposure may increase the risk of hypertension in male coal miners.

Objective: To investigate the prevalence and influencing factors of lower respiratory tract disease. Methods: The health physical examination data of 4000 coal dust exposed workers who had physical examination in the Institute of Occupational Disease Prevention and Treatment in 2016 were collected and analyzed with SPSS 20.0 software. Results: Out of the 4000 coal workers, the Prevalence rate of pulmonary tuberculosis, chronic bronchitis, CWP were 1.00% (40/4000) , 0.63% (25/4000) , 0.43% (17/4000) . 17 coal workers suffered from coal workers. The prevalence of workers both exposed to silicon and coal dust was 1.15% (2/174) , which was higher than that of other the job type. The prevalence of CWP among age groups, length of service and occupational category were found significant difference (P<0.05) , raising with the increase of duration of dust exposure and age increasing (χ²_trend=17.171, 5.344, P<0.05) . The prevalence of emphysema and chronic bronchitis between different working years and different ages are all statistically significant (P<0.05) , increasing with duration of dust exposure (χ²_trend=9.906, 10.118, P<0.05) . Conclusion The prevalence rate of CWP is not high, and closely related with duration of dust exposure and dust species. The prevalence of lower respiratory tract disease is higher in the tunneling and mining workers and raising with the duration of dust exposure.

Objective To explore the association between dust exposure and the incidence of hypertension in male coal miners. Methods Using the method of retrospective cohort study,a hypertension cohort of colliery in Henan Province was established in January 2006. From 2006 to 2017,all the male coal miners in a colliery who were exposed to dust were selected into the exposure group including tunneling, mining,auxiliary and combining workers,and workers from administrative logistics departments who were not exposed to dust were selected into the control group. The eligible participants should satisfy following conditions: working more than one year, with clear and complete record of occupation change, and with complete archives and reliable diagnosis of occupational health surveillance. The exclusion criteria of participants were with hypertension at the baseline of study or with heart,liver,kidney diseases and malignant tumors. A total of 12 647 participants were enrolled in this study (11 663 in the exposure group and 984 in the control group). The follow?up period was from January 2006 to December 2017,with a total follow?up of 89 259.75 person?years. Questionnaires and physical measurements were used to collect general demographic characteristics, occupational exposure history and occupational health surveillance data of all participants. The Cox proportional hazards regression model was used to estimate the association between the dust exposure and the incidence of hypertension. Results During the follow?up period, 2 549 new?onset hypertension patients were identified with an incidence density of hypertension about 2 855.71 per 100 000 person?years. The incidence density of hypertension was 2 967.58 per 100 000 person?years in the exposure group, and 1 643.85 per 100 000 person?years in the control group. The results of multivariate Cox proportional hazards regression model showed that after the adjustment of marriage, age, smoking, alcohol drinking and body mass index,the risk of hypertension was higher in the exposure group compared with the control group ( HR=1.692, 95%CI : 1.410-2.032). Further analysis showed that compared with workers from administrative logistics departments,the risk of hypertension in tunneling,mining and auxiliary working was 1.629(1.345-1.973),1.677(1.374-2.046) and 1.782(1.475-2.151),respectively. Conclusion Dust exposure may increase the risk of hypertension in male coal miners.

Objective To explore the association between dust exposure and the incidence of hypertension in male coal miners. Methods Using the method of retrospective cohort study,a hypertension cohort of colliery in Henan Province was established in January 2006. From 2006 to 2017,all the male coal miners in a colliery who were exposed to dust were selected into the exposure group including tunneling, mining,auxiliary and combining workers,and workers from administrative logistics departments who were not exposed to dust were selected into the control group. The eligible participants should satisfy following conditions: working more than one year, with clear and complete record of occupation change, and with complete archives and reliable diagnosis of occupational health surveillance. The exclusion criteria of participants were with hypertension at the baseline of study or with heart,liver,kidney diseases and malignant tumors. A total of 12 647 participants were enrolled in this study (11 663 in the exposure group and 984 in the control group). The follow?up period was from January 2006 to December 2017,with a total follow?up of 89 259.75 person?years. Questionnaires and physical measurements were used to collect general demographic characteristics, occupational exposure history and occupational health surveillance data of all participants. The Cox proportional hazards regression model was used to estimate the association between the dust exposure and the incidence of hypertension. Results During the follow?up period, 2 549 new?onset hypertension patients were identified with an incidence density of hypertension about 2 855.71 per 100 000 person?years. The incidence density of hypertension was 2 967.58 per 100 000 person?years in the exposure group, and 1 643.85 per 100 000 person?years in the control group. The results of multivariate Cox proportional hazards regression model showed that after the adjustment of marriage, age, smoking, alcohol drinking and body mass index,the risk of hypertension was higher in the exposure group compared with the control group ( HR=1.692, 95%CI : 1.410-2.032). Further analysis showed that compared with workers from administrative logistics departments,the risk of hypertension in tunneling,mining and auxiliary working was 1.629(1.345-1.973),1.677(1.374-2.046) and 1.782(1.475-2.151),respectively. Conclusion Dust exposure may increase the risk of hypertension in male coal miners.

Aim To investigate the effect of ASX (trans-astaxanthin)on the expressions of NF-κB p65 , iNOS and TNF-αin the hippocampus and the prefron-tal cortex of chronic alcohol mice.Methods 40 mice were randomly divided into control group,7 d,14 d, 21 d,28 d alcohol-treated group,the mice were given alcohol preference testing on day of 6,13,20,27. Mice were subjected to alcohol withdrawal for one day after testing.In order to determine the exact time point of cognitive memory impairment in mice after alcohol consumption,they were given morris water maze test after alcohol preference testing. The other 40 mice were randomly divided into control group, alcohol group and ASX group (20,40,80 mg·kg-1 ).After chronic ASX administration, mice were given one probe trial of 60 s in which the platform was removed from the pool to evaluate escape latency,the number of times the animal crossed the previous location of the platform,time spent in the target quadrant,and swim-ming speed.The expressions of NF-κB p65 ,iNOS and TNF-αwere detected by western blotting after behav-ioral testing.Results The mice showed an obvious al-cohol-related phenomenon on 2 1 and 28 days after al-cohol treatment,and escape latency significantly in-creased,entries in target quadrant and duration in tar-get quadrant significantly decreased with increasing drinking days and withdrawal times.The results also suggested that 2 1 days chronic ASX treatment reversed this learning deficit.Moreover,the expression of NF-κB p65 ,iNOS and TNF-αin the hippocampus were significantly increased after 2 1 d alcohol treatment (P<0.001),and pretreatment with ASX (40,80 mg· kg-1 ) could obviously inhibit these changes (P <0.001);Parallel to these changes in the hippocam-pus,the level of NF-κB p65 ,iNOS and TNF-αwere also increased in the prefrontal cortex (P<0.001 ), however,only ASX (80 mg · kg-1 ) administration could inhibit the increase (P<0.05 ).Conclusion These results indicate that ASX pretreatment can pro-tect against alcohol-induced memory impairment via the inhibition of NF- κB p65 ,iNOS and TNF-αexpres-sions in hippocampus and prefrontal cortex.

OBJECTIVE To investigate the effect of ferulic acid(FA)on motor function of rats with a spinal cord injury model(SCI)and its possible effects on expression of microtubule-associated protein light chain 3(LC3),Beclin1,Bcl-2 and Bax. METHODS SD rats were randomly divided into 3 groups:sham group,SCI group,FA(100 mg·kg-1,po)group. Rats were subjected to moderate contusion inju?ries using a vascular clip for 2 min to establish an SCI animal model beforfe they were given BBB scores and inclined plate scoring function test on 6 h,1,3,7 and 14 d after SCI. After the test,rats were sacrificed. Spinal cords were observed by hematoxylin eosin(HE)staining. Furthermore,the expressions of LC3,Beclin1,Bcl-2 and Bax were detected by Western blotting. RESULTS Compared with the sham group,BBB scores and inclined plate function scores significantly decreased in model group. The BBB scores decreased from 21 in sham group to(0.5±0.5)in SCI group,and inclined plate function scores decreased from 70° in sham group to(5.8±2.0)° in SCI group. However,this was reversed by FA treatment. BBB scores and inclined plate function scores increased from 3.0 ± 1.7 to 6.2 ± 3.6(P<0.05)and from (13.3 ± 4.1)° to(26.7 ± 8.7)°(P<0.05)after FA was po given for 7 d,respectively. HE staining showed the gradual emergence of internal spinal cord edema,while the structural changes associated with spinal cord injury could be significantly reversed by administration of FA. Moreover,the expression of LC3-Ⅱ/LC3-Ⅰand Beclin1 was significantly increased in SCI 1 d group(P<0.01),but was decreased in 14 d group when compared with SCI 1 d group(P<0.05). The expression of Bcl-2 was increased in SCI 14 d group,and the ratio of Bcl-2/Bax was increased on 14 d after SCI(P<0.05). Compared with the SCI group,LC3-Ⅱ/LC3-Ⅰand Beclin1 expression was increased in FA-treated 1 d group(P<0.05),Bcl-2 expression was increased in FA-treated 14 d group and the ratio of Bcl-2/Bax was significantly increased on 14 d after SCI(P<0.05). CONCLUSION FA has a therapeutic effect on SCI,which may be related to the impact of neuronal autophagy and apoptosis. Meanwhile,autophagy of SCI may be a process of gradual enhancement followed by weakening,and the anti-apoptosis effect may gradually increase with autophagy.